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mRNA levels of TLR4 and TLR5 are independent of H pylori

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World Journal of Gastroenterology 2008年第34期14卷 5306-5310页

作者： elvira Garza-González Virgilio Bocanegra-García Francisco Javier Bosques-Padilla Juan Pablo Flores-Gutiérrez Francisco moreno Guillermo Ignacio Perez-Perez Departmento de microbiología Facultad de MedicinaUniversidad Autónoma de Nuevo León Departmento de Biología molecular y Bioingeniería UAM Reynosa Aztlán Universidad Autónoma de Tamaulipas Hospital Universitario "Dr. José Eleuterio González" Universidad Autónoma de Nuevo León Department of medicine and microbiology New York University School of Medicine

AIm:To determine if the presence H pylori or its viru- lence affect toll-like receptor 4 (TLR4) and TLR5 mRNA expression levels. mETHODS:For the in vivo assays, gastric biopsies were obtained from 40 patients and H pylori status was determined. For the in vitro assays, human gastric adenocarcinoma mucosal cells (AGS) were cultured in the presence or absence of twelve selected H pylori strains. H pylori strains isolated from culture-positive patients and selected strains were genotyped for cagA and vacA. The cDNA was obtained from mRNA extracted from biopsies and from infected AGS cells. TLR4 and TLR5 mRNA levels were examined by real-time PCR. RESULTS: The presence of H pylori did not affect the mRNA levels of TLR4 or TLR5 in gastric biopsies. The mRNA levels of both receptors were not influenced by the vacA status (P > 0.05 for both receptors) andthere were no differences in TLR4 or TLR5 mRNA levels among the different clinical presentations/histological fi ndings (P > 0.05). In the in vitro assay, the mRNA levels of TLR4 or TLR5 in AGS cells were not influenced by the vacAs1 status or the clinical condition as-sociated with the strains (P > 0.05 for both TLR4 and TLR5). CONCLUSION: The results of this study show that the mRNA levels of TLR4 and TLR5 in gastric cells, both in vivo and in vitro, are independent of H pylori colonization and suggest that vacA may not be a significant player in the first step of innate immune recognition mediated by TLR4 or TLR5.

关键词： H pylori Toll-like receptor 4 Toll-likereceptor 5 AGS cells mRNA

Effect of genistein on acrosome reaction and zona pellucida binding independent of protein tyrosine kinase inhibition in bull

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Asian Journal of Andrology 2007年第5期9卷 650-658页

作者： Viviana A. menzel elvira Hinsch Wolfgang Haigele Klaus-Dieter Hinsch Center of Dermatology and Andrology Justus Liebig University Giessen Giessen D-35392 Germany

Aim： To investigate if the phytoestrogen, genistein, affects essential functions of cryopreserved bovine spermatozoa. methods： The effect of genistein upon motility was assessed by computer-assisted motion analysis. Hemizona assay was performed to detect the ability of spermatozoa binding to the zona pellucida. The inducibility of the acrosome reaction using progesterone and ZP3-6 peptide was analysed by fluorescein-conjugated Pisum sativum agglutinin （FITC-PSA）/Hoechst 33258 double staining. Capacitation after incubation with genistein was assessed by the chlortetracycline （CTC） assay. Immunoblots showed the pattern of protein tyrosine phosphorylation of cryopreserved bovine spermatozoa. Results： Immunodetection of tyrosine-phosphorylated proteins showed that genistein did not affect tyrosine phosphorylation in cryopreserved bovine spermatozoa. However, genistein significantly reduced the progesterone- and ZP3-6 peptide-mediated induction of the acrosome reaction and led to a dose-dependent inhibition of sperm-zona pellucida binding; while sperm motility and capacitation were not affected by this phytoestrogen, as indicated by computer-assisted sperm motion analysis and the CTC assay, respectively. Conclusion： Our results suggest that in cryopreserved bovine spermatozoa, genistein affects a protein tyrosine phosphorylation-independent signal transduction pathway that is involved in sperm capacitation, the acrosome reaction and sperm-zona pellucida binding.

关键词： genistein sperm motility acrosome reaction capacitation tyrosine phosphorylation cryopreservation zona pellucida

Plasma Cell Neoplasms, Clinicopathological Characteristics and Immunophenotype of 21 Patients

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Open Journal of Pathology 2012年第4期2卷 127-132页

作者： mónica Belinda Romero-Guadarrama Cinthia Adriana meza medina elvira Aguilar martínez Facultad de medicina Universidad Autónoma de Nayarit Tepic Mexico Unidad de Hematología del Hospital General de méxico Mexico City Mexico Unidad de Patología del Hospital General de méxico Mexico City Mexico

Introduction: Plasma cell neoplasms are monoclonal proliferations characterized by the secretion of an immunoglobulin product known as component "m" or monoclonal. The World Health Organization (WHO 2008) defines as plasma cell neoplasms the following: plasma cell myeloma, plasmacytoma and those syndromes defined by immunoglobulin deposits and primary amyloidosis, The objective of the present work was to correlate their clinical, morphological and phenotype characteristics in 21 patients. material and methods: A 2-year retrospective review was performed of the files of the surgical pathology laboratory and of the hematology service of the General Hospital of mexico, searching for patients with a diagnosis of plasma cell neoplasm. We analyzed the following variables: age, gender, clinical symptoms, evolution, localization, laboratory tests, morphology, and expression of immunohistochemical markers. Of the 21 patients, 12 (57.1%) corresponded to plasma cell myelomas and 9 (42.8%) were plasmacytomas (seven extraosseous and two solitary bone plasmacytoma);women predominated with 61.4% and age ranged between 22 and 84 years. mass and epistaxis were observed in the patients with plasmacytomas, and symptoms of medullary compression and anemia were observed in those patients with plasma cell myeloma. The time of symptomatology varied from 3 to 12 months. Laboratory tests revealed that lactate dehydrogenase (LDH), beta 2 microglobulin, C-reactive protein were altered and that hypercalcemia and anemia were present more in the systemic form of the disease. Treatment depended on the clinical staging and laboratory data. mature forms predominated morphologically. Immunohistochemical stain revealed a constant expression for CD 138, six patients expressed CD 56, and expression of the Kappa and Lambda light chains was while.

关键词： Plasma Cell Neoplasms

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汉印时态词对比分析与教学

汉印时态词对比分析与教学

作者： elvira SEPTEVANY （邓捷）南昌大学

学位级别：硕士

对于外国留学生来说,汉语时态词是一个常见的,也是相对来说比较难掌握的语法点。相关调查问卷的结果显示,印尼学生对时态词缺乏基本的认识,在使用过程当中,经常会出现这样或那样的错误。带着这个问题,笔者考察了汉印时态词的共同点和不... 详细信息

对于外国留学生来说,汉语时态词是一个常见的,也是相对来说比较难掌握的语法点。相关调查问卷的结果显示,印尼学生对时态词缺乏基本的认识,在使用过程当中,经常会出现这样或那样的错误。带着这个问题,笔者考察了汉印时态词的共同点和不同点,并通过调查问卷分析印尼学生学习汉语时态词时出现的偏误及原因,最后来探讨针对印尼学生行之有效的教学方法。第一章主要论述汉印时态词的共同点。本文写了汉印时态词“着与sedang”、“了与sudah”、“过与pernah”的共同点。汉语与印尼语时态词有一些相似的地方。例如:汉语的“了”与印尼语的“sudah”都可修饰动词表示一个动作的完成。汉语的“着”与印尼语的“sedang”都可修饰动词表示一个动作的持续。汉语的“过”与印尼语的“pernah”都可修饰动词表示一个动作曾经发生等等。第二章主要论述汉印时态词的不同点。通过表格形式列举汉语时态助词“着”、“了”、“过”和印尼语时态副词“sedang”、“sudah”、“pernah”的不同点。在汉语语法中,“着”、“了”、“过”被划分在时态助词的范畴,而在印尼语语法中,这几个词却被划分在时态副词的范畴。第三章主要论述汉印时态词差异的原因。第四章,通过调查问卷的形式分析印尼学生在习得汉语时态词时出现的偏误。被调查者都是南昌大学的印尼学生,人数为20人,调查问题为18个。印尼学生在学习汉语时态词出现了四种频率较高的偏误:误加、遗漏、误用、错位。第五章从教师和学生角度,分别论述了对印尼学生学习汉语时态词的教学建议。

关键词：汉印时态词对比分析偏误教学法

Effect of Compost Based Substrate and mycorrhizal Inoculum in Potted Geranium Plants

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Journal of Life Sciences 2012年第7期6卷 771-775页

作者： monica Tullio Federico Calviello elvira Rea CRA Agricultural Research Council Research Centre for the Soil-Plant System Via Della Navicella 2-4 Rome 00184 Italy

Two are the critical factors in the commercial production of ornamental plants： the substrate and the fertilization method, since the ＂sustainable floriculture＂ discourages the use of synthetic chemical fertilizers and peat-based substrates. The aim of this paper was to test a peat/compost based substrate and a guano ＋ mycorrhizal inoculum fertilizer mixture, in order to obtain a sustainable quality yield of geranium plants. Geranium cuttings （Pelargonium zonale cv. real polaris and Pelargonium grandiflorum cv. lotus） were grown for two months in a glasshouse. Peat, in the growing substrate, was replaced with high quality compost （20% and 40% as reduction percentages）. The fertilizer mixture was made up of guano （3 g/L） and mycorrhizal inoculum （7.5 L/m^3）. Plants of Pelargonium cv. Real polaris, grown on a substrate made up of peat and 20% of high quality compost, presented the better trade features, so to satisfy the ＂sustainable floriculture＂ need.

关键词： Compost-peat horticultural products pelargonium guano mycorrhizal inoculums.

Comparative study measuring the dilatory effect of a mydriatic device(mydriasert~) versus topical drops

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International Journal of Ophthalmology(English edition) 2013年第6期6卷 801-804页

作者： manuel Saenz-de-Viteri Patricia Fernandez-Robredo Elisa de Nova elvira Bonet-Farriol Alfonso L.Sabater Javier Zarranz-Ventura Josemaria Caire Luis m.Sadaba Angel Salinas-Alaman Alfredo Garcia-Layana Department of Ophthalmology University of Navarra

AIm:To compare the mydriatic efficacy of an ophthalmic insert(mydriasert,mY)versus phenylephrine and tropicamide(PT)eye ***:Two controlled,prospective,randomized,single-blind studies were *** the first study,a total of 80 eyes from 40 outpatient-clinic patients were *** drops were applied to the right eye,and a mY device was inserted in the left eye for *** until maximal pupil dilation for each eye was then *** the second study,80 eyes from 80 patients undergoing cataract surgery were *** dilation was achieved using either PT drops three-times for one hour prior to surgery(40 patients),or a mY device was inserted one hour prior to surgery(40 patients).RESULTS:In the first study,mY achieved superior mydriasis compared to PT eye drops at 90min(9.04±1.33mm vs 8.78±1.37mm,P=0.012).However mY took longer than PT drops to achieve maximal dilation,and mydriasis was inferior in eyes with mY compared to PT drops at 30min(7.21±1.73mm vs 8.22±1.43mm,Pming similar by 60min(8.85±1.44mm vs 8.71±1.27mm,P=0.236).In the second study,both mY and PT achieved similar levels of mydriasis at the beginning of surgery(8.75±0.76mm with mY vs 8.77±0.63mm with PT),and also at the end of surgery(7.96±1.06mm with mY vs 8.32±0.72mm with PT),with no significant difference between groups(P=0.08).mY was well tolerated and cardiovascular effects were not influenced by dilation ***:mY could be a safe and efficacious alternative for *** mydriatic effect of mY is as good as conventional PT eye drops after 60min,and is superior after *** also maintains good pupil dilation during cataract surgery.

关键词： mydriasert~ mydriasis pupil dilation cataract surgery device

Regulation of Genetically modified Food Use in the Russian Federation

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Food and Nutrition Sciences 2016年第9期7卷 743-751页

作者： Nadezhda V. Tyshko elvira O. Sadykova Federal State Budgetary Scientific Institution “Federal Research Centre of Nutrition Biotechnology and Food Safety” Moscow Russian Federation

Practical application of new methods of а plant genome transformation resulted in the need for strict regulation of the safety assessment process of genetically modified organisms of plant origin, intended for use in food. The devise of Genetically modified Organisms (GmO) of plant origin safety assessment system, which is currently valid in the Russian Federation, was initiated in 1995-1996. Not only does the system accumulate all domestic and foreign experience, it also includes the latest scientific approaches, based on achievements of fundamental science. In the present article authors focused on issues of the basic legislative regulations of plant biotechnology in the Russian Federation, and approaches to the safety assessment of Gm food and control over such food. GmO safety assessment is carried out at the stage of State registration. The subject of registration is novel food products derived from GmO, manufactured in the Russian Federation, as well as food products, entering the Russian Federation for the first time. In the system of medical and biological assessment of the GmO safety, along with general toxicological research, the study of specific types of toxicity in in vivo experiments takes a prominent place. In accordance with established research practice, which uses an integrated approach, and provides with the most complete and reliable information on potential reprotoxic, genotoxic, immunotoxic and allergenic effects of GmO, as well as enables to reveal possible unintended effects of genetic modification. From 1999 to 2016 22 Gm lines passed the entire cycle of medical-biological investigations. By 2016 there had been amassed scientific foundation on GmO safety, which includes the analysis of result of research, carried out within the framework of GmO state registration in the Russian Federation, as well as domestic and international data, presented by scientific literature on the problem of biotechnological food safety, at both registration stage

关键词： Plant Biotechnology Gm Food GmO Safety Assessment GmO Legislative Regulation Gm Food Labelling Control over GmO

Recovery of Residual Brewer’s Yeast by Electroactivation

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Food and Nutrition Sciences 2021年第12期12卷 1177-1190页

作者： Ruslan Tarna elvira Vrabie Irina Paladii Rodica Sturza Technical University of moldova Chisinau Republic of Moldova Institute of Applied Physics Chisinau Moldova

Yeasts resulting from the brewing process (RBY) are a valuable by-product, with an important content of minerals, vitamins and, especially, proteins. The purpose of the research was the electroactivation of RBY and the simultaneous obtaining of two products—protein concentrates and hydrolyzed protein from residual brewer’s yeast. Electroactivation is a non-residual process, without the use of chemical reagents and relatively inexpensive. The variation of the electroactivation conditions allowed the separation of 90% - 94% of the proteins in the form of protein concentrates. During the process, it is attested to increase the pH value and decrease the redox potential, which characterizes the multiple redox processes that take place in the cathode cell, including sedimentation at the isoelectric point. The presence of albumin in the protein fractions of RBY allows the formation of protein complexes with calcium, attributing a higher biological value to the obtained products.

关键词： Brewer’s Yeast Electroactivation Redox Potential Protein Concentrates Protein Hydrolysates Calcium

Study and Characterization of Organically modified Silica-Zirconia Anti-Graffiti Coatings Obtained by Sol-Gel

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Journal of Chemistry and Chemical Engineering 2013年第2期7卷 120-131页

作者： m. Rosario elvira m. Alejandra mazo Aitana Tamayo Fausto Rubio Juan Rubio Jose Luis Oteo Ceramic and Glass Institute CSIC Madrid 28049 Spain

In this work, it is presented the synthesis and characterization of transparent and colorless organic-inorganic hybrid anti-graffiti protective materials obtained by sol-gel method. This type of materials is based on mTES （methyltriethoxysilane）, TPOZ （tetrapropoxide of zirconium） and PDmS （polydimethylsiloxane）. The synthesis has been carried out at 25, 35 and 45 ℃ in order to evaluate the role of temperature in the structure, microstructure and anti-graffiti behavior as well. The incorporation of zirconium within the organic modified silica network, of sols after being gelled and dried, is evident by a shoulder which increased with temperature situated at 950 cml （Si-O-Zr bonds）, and it is homogenously dispersed inside the matrix avoiding the formation of large ZrO2 precipitates. As the temperature increases, the hydrolysis and condensation reactions occur in more extension and thus, the obtained sols are more cross-linked and present more Si-O-Zr linkages. The promising anti-graffiti beha＇4ior of the protectNe hybrids was qualitatively determined being the spot removal higher than 90%.

关键词： Sol-gel organic-inorganic hybrid anti-graffiti coatings hydrophobic materials.