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Detection of schistosomiasis antibodies in urine patients as a promising diagnostic maker

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Asian Pacific Journal of Tropical Medicine 2011年第10期4卷 773-777页

作者： Salma Medani Elhag Eshtiyag abdalla Abdelkareem Ashraf Siddig Yousif Ehab ahmed Frah ahmed Babiker Mohamed Department of Vector and Biomedical Studies Tropical Medicine Research InstituteNational Centre for ResearchKhartoumSudan Department of Immunology and Biotechnology Tropical Medicine Research InstituteNational Centre for ResearchKhartoumSudan National Centre for Research Ministry of Science and TechnologyKhartoumSudan

Objective:To determine secreted antischistosoma antibodies in urine and to discern the epidemiological situation of schistosomiasis in the agricultural field labourers’camps city in the Gezira State-central ***:Total of 66 urine and 66 serum paired samples were collected from those who confirmed parasitologically positive and negative with schistosomiasis from the two *** were tested using ELBA technique to measure and compare the immunoglobulin G(IgG) levels in serum and urine samples of schistosomiasis ***: The overall prevalence of *** and *** was 53.8%and 15.4%,while the intensity were(2.04 GMEC) and(0.9 GMEC) *** relative percentage of positive IgG individulas in urine was 92.40%where as 96.97%in *** no significant difference between the IgG levels in serum and urine samples was ***:This study shows that the detection of secreted IgG antibodies in urine can substitute serum for diagnosis of schistosomiasis.

关键词： Circulating antibodies Schistosomiasis Immunoglobulin G ELISA

The Possibilities of Chronic Renal Failure Patients Contracting Occult Hepatitis B Virus Infection, Sudan

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Advances in Microbiology 2022年第3期12卷 91-102页

作者： Elhadi abdalla ahmed Abdelrahman Eldaw Mohammed Bakri Yousif Mohamed Nour Albadawi Abdelbagi Talha Zienab Hamid Mubarak ahmed Elshafia Mubarak ahmed Elshafia Mohamed Elamin Salih Department of Medical Microbiology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan Department of Medical Parasitology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan Department of Clinical Laboratory Sciences College of Applied Medical Sciences Al Jouf University Sakakah KSA Department of Haematology and Immunohaematology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan Department of Surgery Faculty of Medicine-AlQunfudtha Umm Al-Qura University Mecca KSA

Introduction: Chronic kidney disease (CKD) is a major cause of death in sub-Saharan Africa. The effects of the CKD on the host and the continuous therapeutic measures increase the hypothesis of blood-borne diseases transmission. Objective: This study aimed to find the frequency of occult hepatitis B virus (OBI) in patients of chronic renal failure (CRF) and to study the possibilities of infection acquisition. Methods: During 2017 and 2019, two hundred CRF patients under regular haemodialysis and attending Gezira Hospital for Renal Diseases and Surgery were recruited. Plasma specimens were collected and used for detection of hepatitis B surface antigens (HbsAg), total hepatitis B core antibodies (anti-HBc) and hepatitis B virus DNA isolation. Nested PCR reaction was followed to identify HBV. Socio-clinical data for each participant was obtained. Results: Male patients represented 64% (128/200), most frequent age group was from 41 to 60 years with percentage of 56.5% (113/200), 86% (172/200) of CRF patients were received blood while 42% (84/200) get HBV vaccination. Hepatitis B core antibodies were found in 54% (108/200) of studied cases, and 22% (42/188) of tested DNA were positively amplified for target gene. Detection of Hepatitis B core antibodies was significantly associated with marital status while absence of vaccination significantly associated with the detection of both hepatitis B core antibodies and HBV DNA. Conclusion: This study found high frequency of OBI in CRF patients, to reduce the transmission of the disease, possible hypotheses should be studied, including blood transfusion, haemodialysis process and HBV vaccination status.

关键词： Chronic Renal Failure OBI Nested PCR Sudan

Hepatocellular injury and the mortality risk among patients with COVID-19: A retrospective cohort study

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World Journal of Hepatology 2021年第8期13卷 939-948页

作者： Ali Madian ahmed Eliwa Hytham abdalla Haitham A Azeem Aly Division of Gastroenterology and Hepatology Department of Internal MedicineFaculty of MedicineAl-Azhar University-AssiutAssiut 71524Egypt Division of Gastroenterology and Hepatology Department of Internal MedicineFaculty of MedicineAl-Azhar University-CairoCairo 11754Egypt Department of Chest Diseases Faculty of MedicineAl-Azhar University-AssiutAssiut 71524Egypt Department of Internal Medicine Faculty of MedicineAl-Azhar University-AssiutAssiut 71524Egypt

BACKGROUND Clearly,infection with severe acute respiratory syndrome coronavirus 2 is not limited to the lung but also affects other *** need predictive models to determine patients’prognoses and to improve health care resource allocation during the coronavirus disease 2019(COVID-19)*** treating COVID-19,we observed differential outcome prediction weights for markers of hepatocellular injury among hospitalized *** To investigate the association between hepatocellular injury and all-cause inhospital mortality among patients with *** This multicentre study employed a retrospective cohort *** adult patients admitted to Al-Azhar University Hospital,Assiut,Egypt and Abo Teeg General Hospital,Assiut,Egypt with confirmed COVID-19 from June 1,2020,to July 30,2020 were *** categorized our cohort into three groups of(1)patients with COVID-19 presenting normal aminotransferase levels;(2)patients with COVID-19 presenting one-fold higher aminotransferase levels;and(3)patients with COVID-19 presenting two-fold higher aminotransferase *** analysed the association between elevated aminotransferase levels and all-cause in-hospital *** survival analysis was performed using the Kaplan–Meier method and tested by log-rank *** In total,376 of 419 patients met the inclusion criteria,while 29(8%)patients in our cohort died during the hospital *** median age was 40 years(range:28-56 years),and 51%were males(n=194).At admission,54%of the study cohort had liver *** pattern of liver injury was hepatocellular injury with an aspartate aminotransferase(AST)*** AST levels were independently associated with all-cause in-hospital mortality in the logistic regression analysis.A one-fold increase in serum AST levels among patients with COVID-19 led to an eleven-fold increase in in-hospital mortality(P<0.001).Admission AST levels correlated with C-reactive protein(r=0.2;P<0.003)and serum fer

关键词： COVID-19 Liver injury Aspartate amino transferase All-cause in-hospital mortality Serum ferritin SARS-CoV-2

Molecular mechanisms of the anti-obesity potential effect of Moringa oleifera in the experimental model

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Asian Pacific Journal of Tropical Biomedicine 2017年第3期7卷 214-221页

作者： Fateheya Mohamed Metwally Hend Mohamed Rashad Hanaa Hamdy ahmed Asmaa ahmed Mahmoud Ehab Ragaa Abdol Raouf Aboelfetoh Mohamed abdalla Environmental and Occupational Medicine National Research CentreGizaEgypt Department of Hormones National Research CentreGizaEgypt Department of Zoology Faculty of ScienceAin Shams UniversityCairoEgypt Department of Research on Children with Special Needs National Research CentreGizaEgypt Department of Horticulture Crops Technology National Research CentreGizaEgypt

Objective: To elucidate the molecular mechanisms of the potent anti-obesity effect of Moringa oleifera Lam.(M. oleifera) ethanolic extract and to clarify the link between these mechanisms and the associated metabolic and vascular risks in the experimental model of visceral ***: M. oleifera ethanolic extract was orally administered at 600 mg/kg body weight in obese female rats daily for 12 weeks. At the end of treatment, body weight was determined, and the atherogenic index, coronary artery index, glucose level, insulin resistance status, liver and kidney functions were assessed. Also, the m RNA of leptin, adiponectin and resistin in visceral adipose tissue was determined by quantitative real ***: The results showed that M. oleifera extract down-regulated m RNA expression of leptin and resistin, while it up-regulated adiponectin gene expression in obese rats relative to untreated obese control counterparts. This amelioration of genes expression was paralleled by a reduction in body weight and improvement of the atherogenic index and coronary artery index, as well as glucose level and insulin resistance value without adverse effects on liver or kidney functions, versus the untreated obese control ***: It is reasonable to assume that the anti-obesity, anti-atherogenic and antidiabetic properties of M. oleifera are mechanistically achieved via working directly on the adipokines of the visceral adipose tissue. Therefore, M. oleifera may be a good therapeutic candidate for the symptoms of metabolic syndrome.

关键词： Moringa oleifera Lam Visceral adipose tissue Adipokines Gene expression Rats

Adaptive Power Control Aware Depth Routing in Underwater Sensor Networks

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Computers, Materials & Continua 2021年第10期69卷 1301-1322页

作者： Ghufran ahmed Saiful Islam Ihsan Ali Isra Adil Hayder Abdelmuttlib Ibrahim abdalla ahmed Muhammad Talha Sultan S.Alshamrani Ag Asri Ag Ibrahim Department of Computer Science FAST National University of Computer and Emerging Sciences(NUCES)KarachiPakistan Dr.A.Q.Khan Institute of Computer Sciences and Information Technology KahutaPakistan Faculty of Computer Science and Information Technology University of MalayaKuala Lumpur50603Malaysia Department of Computer Science COMSATS University IslamabadIslamabadPakistan Deanship of Scientific Research King Saud UniversityRiyadhSaudi Arabia Department of Information Technology College of Computer and Information TechnologyTaif UniversityTaif21944Saudi Arabia Faculty of Computing and Informatics Universiti Malaysia SabahKota KinabaluSabahMalaysia

Underwater acoustic sensor network(UASN)refers to a procedure that promotes a broad spectrum of aquatic *** can be practically applied in seismic checking,ocean mine identification,resource exploration,pollution checking,and disaster *** confronts many difficulties and issues,such as low bandwidth,node movements,propagation delay,3D arrangement,energy limitation,and high-cost production and arrangement costs caused by antagonistic underwater *** wireless sensor networks(UWSNs)are considered a major issue being encountered in energy management because of the limited battery power of their ***,the harsh underwater environment requires vendors to design and deploy energy-hungry devices to fulfil the communication requirements and maintain an acceptable quality of ***,increased transmission power levels result in higher channel interference,thereby increasing packet *** the facts mentioned above,this research presents a controlled transmission power-based sparsity-aware energy-efficient clustering in *** contributions of this technique is ***,it uses the adaptive power control mechanism to utilize the sensor nodes’battery and reduce channel interference ***,thresholds are defined to ensure successful ***,clustering can be implemented in dense areas to decrease the repetitive transmission that ultimately affects the energy consumption of nodes and interference ***,mobile sinks are deployed to gather information locally to achieve the previously mentioned *** suggested protocol is meticulously examined through extensive simulations and is validated through comparison with other advanced UWSN *** show that the suggested protocol outperforms other procedures in terms of network lifetime and packet delivery ratio.

关键词： UWSNs UASNs QoS

The Expression of miRNAs in Sudanese Patients with Systemic Lupus Erythematosus in Khartoum State

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Open Journal of Rheumatology and Autoimmune Diseases 2022年第4期12卷 128-136页

作者： Sarah Mohammed Dafalla ahmed Elhadi Elsadig Tarig A. M. Hamid Huda Mohamed Haroun Sana Eltahir abdalla Department of Haematology Medical Laboratory Sciences Program Alyarmouk College Khartoum Sudan Department of Haematology Faculty of Medical Laboratory Sciences Alzaiem Al Azhari University Khartoum Sudan Department of Haematology and Immunohematology Sharq El Nile College Khartoum North Sudan Department of Haematology Faculty of Medical Laboratory Sciences University of Gezira Madani Sudan Department of Pathology Faculty of Medicine Al Neelain University Khartoum Sudan

Background: MicroRNAs (miRs) are noncoding gene regulators that may have a role as diagnostic or prognostic biomarkers in systemic lupus erythematosus (SLE). Aim: To measure the blood levels of miR-146a, miR-126 and miR-30a in Sudanese SLE patients and to investigate their potential role in disease pathogenesis and utility as biomarkers for SLE. Material and Methods: A total of 48 SLE patients and 20 matched healthy individuals were enrolled in this study. SLE disease activity index (SLEDAI) was assessed. The blood levels of miR-146a, miR-126 and miR-30a were determined by Real-time polymerase chain reaction (PCR) in all participants. Γ-INF and IL-2 were analyzed by ELISA, and CD markers were used in flow cytometry. Results: The mean age of the patients was 31.5 ± 8.5 years with disease duration > 5 years. In SLE patients, the mean blood level fold changes of miR-146a (0.33 ± 0.277;P 0.305) compared to controls. Down regulation of miR-146a increase expression of γ-INF (P 0.05) as no differences between the SLE patients and healthy control. Conclusion: Circulating miR-146a and miR-126 could be a potential noninvasive biomarker in SLE. This study provides an overview of the current state of research on the role of miRNAs in the immune pathogenesis and regulation of SLE. Further studies are needed in miRNAs profiling expressions of SLE diseases.

关键词： Systemic Lupus Erythematosus (SLE) MicroRNAs

Nanothermite colloids: A new prospective for enhanced performance

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Defence Technology（防务技术） 2019年第3期15卷 319-325页

作者： M. Gaber Zaky ahmed M. abdalla Rakesh P. Sahu Ishwar K. Puri Mostafa Radwan Sherif Elbasuney School of Chemical Engineering Military Technical College Kobry Elkoba Cairo Egypt Department of Engineering Physics and Department of Mechanical Engineering McMaster University 1280 Main Street West Hamilton Ontario L8S 4L7 Canada Department of Mechanical Engineering McMaster University 1280 Main Street West Hamilton Ontario L8S 4L7 Canada British University in Egypt Elshorouk City Cairo Egypt

Nanothermites (metal oxide/metal) can offer tremendously exothermic self sustained reactions. CuO is one of the most effective oxidizers for naonothermite applications. This study reports on two prospectives for the manufacture of CuO nanoparticles. Colloidal CuO particles of 15 nm particle size were developed using hydrothermal synthesis technique. Multiwalled carbon nanotubes (MWCNTs) with surface are 700m2/g was employed as a substrate for synthesis of CuO-coated MWCNTs using electroless plating. On the other hand, aluminium particles with combustion heat of 32000 J/g is of interest as high energy density material. The impact of stoichiometric nanothermite particles (CuO/Al & Cuo-coated MWCNTs/Al) on shock wave strength of Al/TNT nanocomposite was evaluated using ballistic mortar test. While CuO-coated MWCNTs decreased the shock wave strength by 15%;colloidal CuO enhanced the shock wave strength by 30%. The superior performance of colloidal CuO particles was correlated to their steric stabilization with employed organic solvent. This is the first time ever to report on fabrication, isolation, and integration of stablilized colloidal nanothermite particles into energetic matrix where intimate mixing between oxidizer and metal fuel could be achieved.

关键词： Hydrothermal synthesis Nanoparticles Carbon nanotubes Nanothermites Energetic materials

Diagnostic Performance of glmM Gene and Histological Stains for Detection of Helicobacter pylori in Gastric Biopsy from Patients Admitted to Wad Madani Teaching Hospital, Sudan

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Natural Science 2022年第8期14卷 343-352页

作者： Karima Ali Hamid Albadawi Abdelbagi Talha Abdalraheem Ali Babiker Mohammed Ibrahim Malik Adam Dawoud Abakar Omer Mustafa Elsidig A Saeed Mohmed Bushra ahmed Elhadi abdalla ahmed Department of Histopathology and Cytology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan Department of Clinical Laboratory Sciences College of Applied Medical Sciences Jouf University Al Jouf Kingdom of Saudi Arabia Department of Medicine Faculty of Medicine University of Gezira Wad Medani Sudan Department of Medical Microbiology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan

Helicobacter pylori is the microbial agent most responsible for gastro-duodenal ulcer and chronic gastritis, which can develop into carcinoma of the stomach. This study was performed in Wad Medani Teaching Hospital, Sudan to detect Helicobacter pylori in stomach samples, and evaluate the performance of the tests used, which were histological stains and PCR. Gastric biopsies were obtained from 105 referred patients during endoscopy, and fixed specimens examined by haematoxylin-eosin and Warthin-Starry silver stains, while DNA was extracted for glmM gene amplification. Epigastric pain was the most common symptom at 78% (82/105) and chronic gastritis recorded with 71% (68/105) of endoscopy results. Warthin-Starry silver stain gave 31% (33/105) as positive for Helicobacter pylori followed by glmM gene 27% (28/105) and haematoxylin-eosin 24% (25/105). The study indicated good performance of histological staining and high specificity of glmM gene in detection of Helicobacter pylori from gastric biopsies.

关键词： Helicobacter pylori Histological Stains glmM Gene Gastric Biopsy Sudan

Genetic Diversity Analysis of Elite Doubled Haploid Rice Genotypes for Yield Attributing Traits in White Nile State, Sudan

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Agricultural Sciences 2022年第3期13卷 330-344页

作者： Khalid A. Osman Sara M. abdalla Kyung-Ho Kang Lee Sang-Bok ahmed E. Mohamed Yassir M. ahmed Amir I. Ismial Agricultural Research Corporation (ARC) Rice Breeding Program White Nile Research Station Kosti Sudan National Institute of Crop Science Rural Development Administration (RDA) South Korea & AfricaRice Saint-Louis Senegal

The development of new rice varieties is highly dependent on genetic diversity in desirable agronomic traits. Therefore, this study aimed to identify potential genotypes having the characters of Korean varieties (Tongil-type) and japonica developed through doubled haploid (DH) technology to apply in our breeding materials. 35 elite DH lines derived from another culture of Korean and African rice along with two local checks were planted in a randomized complete block design with three replications during the two seasons of 2019 and 2020. All evaluated genotypes exhibited a wide and significant variation in the ten measured traits. The highest heritability related to high genetic advance was recorded for the number of tiller/plant, grain yield t/ha, number of filled grain per panicle, and thousand grain weights (g). Genotypic coefficient of variation and genetic advance were recorded for number of tiller/plant, number of filled grain per panicle, 1000 grain weight and grain yield t/ha in both seasons. Moreover, there was a highly significant and positive correlation of grain yield with number of filled grain per panicle (0.65), number of tiller/plant (0.64) and number of panicle per m2 (0.54). Cluster analysis based on grain yield components trait grouped the 37 rice genotypes into four clusters. Cluster B was the largest and consisted of 13 genotypes. Finally, it could be concluded that, based on number of productive tillers, number of filled grain/panicle, number of panicle per m2, and grain yield, the lines KF170506, KF170509, KF170542, KF170530, KF170543, KF170500 and KF170510 were high potential for further selection for new type of irrigated rice. In addition, hybridization of these 7 high-yielding could be used to achieve higher heterosis among the genotypes. Furthermore, this evaluation could be useful in developing reliable selection indices for improving rice breeding programs.

关键词： Doubled Haploid Anther Culture Yield Components Heritability and Correlation