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Detection of cardiac myosin binding protein-C (cMyBP-C) by a phospho-specific PKD antibody in contracting rat cardiomyocytes

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Advances in Bioscience and Biotechnology 2013年第4期4卷 1-6页

作者： ellen dirkx Freek G. Bouwman Didier Vertommen Edwin C. Mariman Sakthivel Sadayappan Jan F. C. Glatz Joost J. F. P. Luiken Guillaume J. van Eys Department of Cell and Molecular Physiology Stritch School of Medicine Loyola University Chicago USA Department of Human Biology Nutrition and Toxicology Research Institute Maastricht (NUTRIM) Maastricht University Maastricht The Netherlands Department of Molecular Genetics Cardiovascular Research Institute Maastricht (CARIM) Maastricht University Maastricht The Netherlands Protein Phosphorylation Unit Université Catholique de Louvain Brussels Belgium

Protein phosphorylation plays an important role in physiological processes, such as muscle contraction. Phospho-specific antibodies have become powerful tools to study these processes. Cardiac myosin binding protein-C (cMyBP-C) is one of the proteins that make up the contractile apparatus of cardiomyocytes. Phosphorylation of cMyBP-C is essential for normal cardiac function, since dephosphorylation of this protein leads to its degradation and has been associated with cardiomyopathy. One of the upstream kinases, which phosphorylate cMyBP-C, is protein kinase D (PKD). While studying the role of PKD in cMyBP-C phosphorylation, we tried to analyze phosphorylation of PKD with a phospho-specific PKD-Ser744/748 antibody. Contrary to the expected 115 kDa, a signal was found for a 150-kDa protein. By MALDI-TOF mass spectrometry, we identified this protein to be cMyBP-C. These data were confirmed by immunostaining using the p-PKD-Ser744/748 antibody, which displayed a striated pattern similar to the one observed for a regular cMyBP-C antibody. To our knowledge there are no antibodies commercially available for phosphorylated cMyBP-C. Thus, the p-PKD-Ser744/748 antibody can accelerate research into the role of cMyBP-C phosphorylation in cardiomyocytes.

关键词： Protein Kinase D Cardiac Myosin Binding Protein-C Phospho-Specific Antibody Protein Phosphorylation

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New glucocerebrosidase antibodies can advance research in the field of neurodegenerative disorders

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Neural Regeneration Research 2025年第4期20卷 1085-1086页

作者： Charis Ma Krystyna Rytel Yu Chen ellen Sidransky Medical Genetics Branch National Human Genome Research InstituteNational Institutes of HealthBethesdaMDUSA

In medical research,there are times when the introduction of a new tool can launch scientific discovery in new *** antibody development may be considered mundane,in the field of glucocerebrosidase(GCase)research,the dearth of validated antibodies for different applications has impeded progress in studies of disease pathogenesis and therapeutic *** recent introduction of new,rigorously evaluated antibodies can now propel research into the link between glucocerebrosidase and Parkinson’s disease(PD)as well as aspects of the pathobiology of Gaucher disease(Jong et al.,2024).

关键词： antibodies cerebro

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MicroRNAs in inflammatory bowel disease:What do we know and what can we expect?

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World Journal of Gastroenterology 2024年第16期30卷 2184-2190页

作者： ellen Cristina Souza de Oliveira Ana Elisa Valencise Quaglio Thais Gagno Grillo Luiz Claudio Di Stasi Ligia Yukie Sassaki Department of Internal Medicine Medical SchoolSão Paulo State University(Unesp)Botucatu 18618-686São PauloBrazil Verum Ingredients Botucatu Technology ParkBotucatu 18605-525São PauloBrazil Department of Biophysics and Pharmacology Institute of BiosciencesSão Paulo State University(Unesp)Botucatu 18618-689São PauloBrazil

MicroRNAs(miRNAs),small non-coding RNAs composed of 18–24 nucleotides,are potent regulators of gene expression,contributing to the regulation of more than 30%of protein-coding *** that miRNAs are regulators of inflammatory pathways and the differentiation of intestinal epithelial cells,there is an interest in exploring their importance in inflammatory bowel disease(IBD).IBD is a chronic and multifactorial disease of the gastrointestinal tract;the main forms are Crohn's disease and ulcerative *** studies have investigated the dysregulated expression of miRNAs in IBD,demonstrating their important roles as regulators and potential biomarkers of this *** editorial presents what is known and what is expected regarding miRNAs in *** the important regulatory roles of miRNAs in IBD are clearly established,biomarkers for IBD that can be applied in clinical practice are lacking,emphasizing the importance of further *** regarding the influence of miRNAs on the inflammatory process and the exploration of their role in gene regulation are expected to provide a basis for the use of miRNAs not only as potent biomarkers in IBD but also as therapeutic targets for the control of inflammatory processes in personalized medicine.

关键词： MicroRNAs Inflammatory bowel disease Crohn’s disease Ulcerative colitis Biomarker Therapy

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Liver abnormalities following SARS-CoV 2 infection in children 1 to 10 years of age

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Family Medicine and Community Health 2024年第1期12卷 28-35页

作者： Pauline Terebuh Veronica R Olaker ellen K Kendall David C Kaelber Rong Xu Pamela B Davis Center for Artificial Intelligence in Drug Discovery Case Western Reserve UniversityClevelandOHUSA The Center for Clinical Informatics Research and Education The MetroHealth SystemClevelandOHUSA Department of Medicine PediatricsDepartment of Population and Quantitative Health SciencesCase Western Reserve UniversityClevelandOHUSA Center for Community Health Integration Case Western Reserve UniversityClevelandOHUSA

Objective Beginning in October 2021 in the USA and elsewhere,cases of severe paediatric hepatitis of unknown aetiology were identified in young *** the adenovirus and adenovirus-associated virus have emerged as leading aetiological suspects,we attempted to investigate a potential role for SARS-CoV 2 in the development of subsequent liver *** We conducted a study using retrospective cohorts of deidentified,aggregated data from the electronic health records of over 100 million patients contributed by US healthcare *** Compared with propensity score matched children with other respiratory infections,children aged 1-10 years with COVID-19 had a higher risk of elevated transaminases(HR(95%CI)2.16(1.74 to 2.69))or total bilirubin(HR(95%CI)3.02(1.91 to 4.78)),or new diagnoses of liver diseases(HR(95%CI)1.67(1.21 to 2.30))from 1 to 6 months after *** with pre-existing liver abnormalities,liver abnormalities surrounding acute infection,younger age(1-4 years)or illness requiring hospitalisation all had similarly elevated *** who developed liver abnormalities following COVID-19 had more pre-existing conditions than those who developed abnormalities following other *** These results indicate that SARS-CoV 2 may prime the patient for subsequent development of liver infections or non-infectious liver *** rare(~1 in 1000),SARS-CoV 2 is a risk for subsequent abnormalities in liver function or the diagnosis of diseases of the liver.

关键词： elevated diagnosis subsequent

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Association of Serum Folate and Vitamin B_(12) Concentrations with Obesity in Chinese Children and Adolescents

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Biomedical and Environmental Sciences 2024年第3期37卷 242-253页

作者： ZHU Qian Rang KOK E Dieuwertje BEKELE Hailu Tesfaye MANUSAMA Koen ZHANG Jing Xian XIE Wei ZONG Wen Qi ZUO Hui ZHANG Jian KAMPMAN ellen DAI Yue Jiangsu Provincial Center for Disease Control and Prevention Nanjing 210009JiangsuChina Division of Human Nutrition and Health Wageningen University&ResearchWageningen 176700 AAThe Netherlands School of Public Health Medical College of Soochow UniversitySuzhou 215123JiangsuChina National Institute for Nutrition and Health Chinese Center for Disease Control and PreventionBeijing 100050China

Objective This study aimed to evaluate the associations of serum folate and/or vitamin B12 concentrations with obesity among Chinese children and *** A cross-sectional study was conducted including 3,079 Chinese children and adolescents,aged 6 to 17 years,from Jiangsu,*** indices,such as,children's body mass index(BMI),BMI z-scores,waist circumference,and waist-to-height ratio were *** linear regression and generalized additive models were used to investigate the associations of serum folate and vitamin B12 levels with anthropometric indices and odds of *** We observed that serum vitamin B12 concentrations were inversely associated with all anthropometric indices and the odds of general obesity[odds ratio(OR)=0.68;95%confidence interval(CI)=0.59,0.78]and abdominal obesity(OR=0.68;95%CI=0.60,0.77).When compared to participants with both serum vitamin levels in the two middle quartiles,those with both serum folate and vitamin B12 levels in the highest quartile were less prone to general(OR=0.31,95%CI=0.19,0.50)or abdominal obesity(OR=0.46,95%CI=0.31,0.67).Conversely,participants with vitamin B12 levels in the lowest quartile alongside folate levels in the highest quartile had higher odds of abdominal obesity(OR=2.06,95%CI=1.09,3.91).Conclusion Higher serum vitamin B12 concentrations,but not serum folate concentrations,were associated with lower odds of childhood *** and adolescents with high levels of vitamin B12 and folate were less likely to be obese.

关键词： Folate Vitamin B12 Childhood obesity Micronutrient

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AI有望解锁古罗马卷轴之谜

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商业周刊（中文版） 2024年第15期 52-57页

作者： Ashlee Vance ellen Huet 杨飞(译) 不详

几年前,在加利福尼亚州日益恶化的一个野火季,纳特·弗里德曼(Nat Friedman)家的房子被烧毁了。几个月后,弗里德曼因为新冠疫情被困在湾区,既煌恐又无聊。和许多中年老父亲一样,他从古罗马文化当中寻求安慰和指引。当我们中的一些人正... 详细信息

几年前,在加利福尼亚州日益恶化的一个野火季,纳特·弗里德曼(Nat Friedman)家的房子被烧毁了。几个月后,弗里德曼因为新冠疫情被困在湾区,既煌恐又无聊。和许多中年老父亲一样,他从古罗马文化当中寻求安慰和指引。当我们中的一些人正在观看《虎王》(Tiger King)或者玩孩子们的乐高积木时,他在阅读关于罗马帝国的书籍,还帮他女儿制作了罗马别墅的纸模型。他没有学着做酸面团。

关键词：古罗马文化弗里德曼加利福尼亚州纸模型乐高积木罗马帝国

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管道清洁机器人的创新应用

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现代制造 2024年第1期 27-29页

作者： Andreas Seegen ellen-Christine Rei 不详

地下排水管道需要定期的维护保养,而且最好是在不挖开地面的情况下进行维护保养。为此,Pipetronics公司开发了一种可对地下管道进行维护保养的机器人。这款机器人由Faulhaber公司研发生产的无刷直流电机来驱动机器人车轮运转,驱动机器... 详细信息

地下排水管道需要定期的维护保养,而且最好是在不挖开地面的情况下进行维护保养。为此,Pipetronics公司开发了一种可对地下管道进行维护保养的机器人。这款机器人由Faulhaber公司研发生产的无刷直流电机来驱动机器人车轮运转,驱动机器人的清洁工具完成地下管道的清洁。

关键词：机器人地下管道维护保养 Pipe 无刷直流电机创新应用清洁工具

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A chromosome-scale genome assembly of Rauvolfia tetraphylla facilitates identification of the complete ajmaline biosynthetic pathway

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Plant Communications 2024年第4期5卷 129-132页

作者： Enzo Lezin Ines Carqueijeiro Clement Cuello Mickael Durand Hans JJansen Valentin Verges Caroline Birer Williams Audrey Oudin Thomas Duge de Bernonville Julien Petrignet Noemie Celton Benoit St-Pierre Nicolas Papon Chao Sun Ron P.Dirks Sarah ellen O’Connor Michael Krogh Jensen Sebastien Besseau Vincent Courdavault Biomole´cules et Biotechnologies Vegetales EA2106Universite´de Tours37200 ToursFrance Future Genomics Technologies 2333 BE Leidenthe Netherlands Laboratoire Synthe se et Isolement de Molecules BioActives(SIMBA EA 7502)Universitede Tours37200 ToursFrance Laboratoire de Cytoge´netique Constitutionnelle CHRU de Tours-Hopital Bretonneau37044 ToursFrance University Angers University BrestIRFSFR ICAT49000 AngersFrance Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 2800China Department of Natural Product Biosynthesis Max Planck Institute for Chemical Ecology07745 JenaGermany Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark100193 Kgs LyngbyDenmark Present address:Limagrain Center de RechercheRoute d’EnnezatChappesFrance These authors contributed equally to this article

Dear Editor,Rauvolfia tetraphylla(aka the Devil pepper)(Supplemental Figure 1)is a well-known medicinal plant that produces monoterpenoid indole alkaloids(MIAs).This MIA biosynthesis occurs in several organs,including leaves,stems,fruit,and roots,which accumulate the famous antiarrhythmic ajmaline(Kumar et al.2016a,2016b;Kumara et al.,2019).MIAs are natural products notably involved in plant adaptation to the environment and defense against *** mainly results from their high biological activities,which also explain their pharmacological properties.

关键词： properties. Figure roots

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永生的信徒

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商业周刊（中文版） 2024年第2期 46-51页

作者： ellen Huet lan Loring Shiver(摄影) 王湛(译) 不详

当一个拿着夹纸写字板的人在旧金山街头热情地向你走来时,你通常可以预料到,他们的问题与某个公共政策目标有关。您有时间来谈谈堕胎权吗?住房问题?网络赌博投票倡议?然而如今,你可能会听到一个更加宏大、更加个人的问题,你怎么也想不到... 详细信息

当一个拿着夹纸写字板的人在旧金山街头热情地向你走来时,你通常可以预料到,他们的问题与某个公共政策目标有关。您有时间来谈谈堕胎权吗?住房问题?网络赌博投票倡议?然而如今,你可能会听到一个更加宏大、更加个人的问题,你怎么也想不到,那些看起来想要你签名的人会问出这句话:您想活多久?

关键词：写字板网络赌博住房问题公共政策目标旧金山

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CD4^(+)T cells produce IFN-I to license cDC1s for induction of cytotoxic T-cell activity in human tumors

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Cellular & Molecular Immunology 2024年第4期21卷 374-392页

作者： Xin Lei Daniël C.de Groot Marij J.P.Welters Tom de Wit ellen Schrama Hans van Eenennaam Saskia J.Santegoets Timo Oosenbrug Annemarthe van der Veen Joris L.Vos Charlotte L.Zuur Noel F.C.C.de Miranda Heinz Jacobs Sjoerd H.van der Burg Jannie Borst Yanling Xiao Department of Immunology Leiden University Medical CenterLeidenThe Netherlands Oncode Institute Leiden University Medical CenterLeidenThe Netherlands Department of Tumor Biology and Immunology The Netherlands Cancer InstituteAmsterdamThe Netherlands Department of Medical Oncology Oncode InstituteLeiden University Medical CenterLeidenThe Netherlands IMMIOS B.V. OssThe Netherlands Division of Medical Oncology The Netherlands Cancer InstituteAmsterdamThe Netherlands Department of Otorhinolaryngology Leiden University Medical Center LeidenThe Netherlands Department of Pathology Leiden University Medical CenterLeidenThe Netherlands

CD4^(+)T cells can"help"or"license" conventional type 1 dendritic cells(cDC1s)to induce CD8^(+)cytotoxic T lymphocyte(CTL)anticancer responses,as proven in mouse *** recently identified cDC1s with a transcriptomic imprint of CD4^(+)T-cell help,specifically in T-cell-infiltrated human cancers,and these cells were associated with a good prognosis and response to PD-1-targeting ***,we delineate the mechanism of cDC1 licensing by CD4^(+)T cells in *** CD4^(+)T cells produce IFNβvia the STING pathway,which promotes MHC-I antigen(cross-)presentation by cDC1s and thereby improves their ability to induce CTL anticancer *** cooperation with CD40 ligand(L),IFNβalso optimizes the costimulatory and other functions of cDC1s required for CTL response ***-I-producing CD4^(+)T cells are present in diverse T-cell-infiltrated cancers and likely deliver“help”signals to CTLs locally,according to their transcriptomic profile and colocalization with“helped/licensed”cDCs and tumor-reactive CD8^(+)T *** agreement with this scenario,the presence of IFN-I-producing CD4^(+)T cells in the TME is associated with overall survival and the response to PD-1 checkpoint blockade in cancer patients.

关键词： CD4^(+)T-cell help cDC1 licensing IFN-I signaling CD40 costimulation (cross-)presentation CTL priming Tumor control

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