Tumor cells have an increased demand for glucose and amino acids to support their rapid growth,and also exhibit alterations in biochemical pathways that metabolize these *** across the plasma membrane is essential to ...
详细信息
Tumor cells have an increased demand for glucose and amino acids to support their rapid growth,and also exhibit alterations in biochemical pathways that metabolize these *** across the plasma membrane is essential to feed glucose and amino acids into these tumor cell-selective metabolic *** of amino acids across biological membranes occurs via a multitude of transporters;tumor cells must upregulate one or more of these transporters to satisfy their increased demand for amino *** the amino acid transporters,SLC6A14 stands out with specific functional features uniquely suited for the biological needs of the tumor *** transporter is indeed upregulated in tumors of epithelial origin,including colon cancer,cervical cancer,breast cancer,and pancreatic *** normal cells express this transporter only at low levels,blockade of this transporter should lead to amino acid starvation selectively in tumor cells,thus having little effect on normal *** offers a novel,yet logical,strategy for the treatment of cancers that are associated with upregulation of *** addition,a variety of amino acid-based prodrugs are recognized as substrates by SLC6A14,thus raising the possibility that anticancer drugs can be delivered into tumor cells selectively via this transporter in the form of amino acid *** strategy allows exposure of SLC6A14-positive tumor cells to chemotherapy with minimal off-target *** conclusion,the amino acid transporter SLC6A14 holds great potential not only as a direct drug target for cancer therapy but also for tumor cell-selective delivery of anticancer drugs.
Based on the evidence that hemochromatosis, an iron-overload disease, drives hepatocellular carcinoma, we hypothesized that chronic exposure to excess iron, either due to genetic or environmental causes, predisposes a...
详细信息
Based on the evidence that hemochromatosis, an iron-overload disease, drives hepatocellular carcinoma, we hypothesized that chronic exposure to excess iron, either due to genetic or environmental causes, predisposes an individual to cancer. Using pancreatic cancer as our primary focus, we employed cell culture studies to interrogate the connection between excess iron and cancer, and combined in vitro and in vivo studies to explore the connection further. Ferric ammonium citrate was used as an exogenous iron source. Chronic exposure to excess iron induced epithelial-mesenchymal transition(EMT) in normal and cancer cell lines, loss of p53, and suppression of p53 transcriptional activity evidenced from decreased expression of p53 target genes(p21, cyclin D1, Bax, SLC7A11). To further extrapolate our cell culture data, we generated EL-KrasG12D( EL-Kras) mouse(pancreatic neoplastic mouse model) expressing Hfe+/+ and Hfe-/- genetic background. p53 target gene expression decreased in EL-Kras/Hfe-/- mouse pancreas compared to EL-Kras/Hfe+/+ mouse pancreas. Interestingly, the incidence of acinar-to-ductal metaplasia and cystic pancreatic neoplasms(CPN) decreased in EL-Kras/Hfe-/- mice, but the CPNs that did develop were larger in these mice than in EL-Kras/Hfe+/+ mice. In conclusion, these in vitro and in vivo studies support a potential role for chronic exposure to excess iron as a promoter of more aggressive disease via p53 loss and SLC7A11 upregulation within pancreatic epithelial cells.
During Covid pandemic,many individuals are suffering from suicidal ideation in the *** distancing and quarantining,affects the patient *** computing is the study of recognizing human feelings and *** technology can be...
详细信息
During Covid pandemic,many individuals are suffering from suicidal ideation in the *** distancing and quarantining,affects the patient *** computing is the study of recognizing human feelings and *** technology can be used effectively during pandemic for facial expression recognition which automatically extracts the features from the human *** system plays a very important role to detect the patient condition and to recognize the patterns of expression from the safest *** this paper,a new method is proposed for emotion recognition and suicide ideation detection in COVID *** helps to alert the nurse,when patient emotion is fear,cry or *** research presented in this paper has introduced Image Processing technology for emotional analysis of patients using Machine learning *** proposed Convolution Neural Networks(CNN)architecture with DnCNN preprocessing enhances the performance of *** system can analyze the mood of patients either in real time or in the form of video files from CCTV *** proposed method accuracy is more when compared to other *** detects the chances of suicide attempt based on stress level and emotional recognition.
暂无评论