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Application of multiphoton microscopy in dermatological studies: A mini-review

Journal of Innovative Optical Health Sciences 2014年第5期7卷 39-63页

作者： eljah yew Christopher R.owlands Peter T.C.So Singapore-MIT Alliance for Researchand Technology(SMART) 1 CREATE WayCREATE TowerSingapore 138602 Department of Biological Engineering Massachusetts Institute of Technology 77 Massachusetts Ave Cambridge MA 02139USA Department of Mechanical Engineering Massachusetts In.stitute of Technology 77 Massachusetts Ave Cambridge MA 02139USA SGR Harrison Spectroscopy Laboratory 77 Massachusetts Ave Cambridge MA 02139USA

This review summarizes the historical and more recent developments of multiphoton microscopy,as applied to *** microscopy offers several advantages over competingmicroscopy techniques:there is an inherent axial sectioning,penetration depths that competewll with confocal microscopy on account of the use of near-infrared light,and many t wo-pho ton contrast mechanisms,such as second-harmonic generation,have no analogue in *** the penetration depths of photons into tissue are typically limited on the orderof hundreds of microns,this is of les concern in dermatology,as the skin is thin and *** a result,multiphoton microscopy in dermatology has generated a great deal ofmterest,much of which is summarized *** review covers the interaction of light and tissue,as well as the various considerations that must be made when designing an *** stateof multiphoton microscopy in imaging skin cancer and various other diseases is also discussed,along with the investigation of aging and regeneration phenomena,and finally,the use of mul-tiphoton microscopy to analyze the transdermal transport of drugs,cosmetics and other agents *** review concludes with a look at potential future research directions,especiallythose that are necessary to push these techniques into widespread clinical acceptance.

关键词： Multiphoton microscopy skin cancer,aging and regeneration.

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