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Interrelationship between chromosome 8 aneuploidy,C-MYC amplification and increased expression in individuals from northern Brazil with gastric adenocarcinoma

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World Journal of Gastroenterology 2006年第38期12卷 6207-6211页

作者： Danielle Queiroz Calcagno Mariana Ferreira Leal Aline Damaceno Seabra Andre Salim Khayat elizabeth suchi chen Samia Demachki Paulo Pimentel Assumpcao Mario Henrique Girao Faria Silvia Helena Barem Rabenhorst Márcia Valéria Pitombeira Ferreira Marília de Arruda Cardoso Smith Rommel Rodríguez Burbano Human Cytogenetics and Toxicological Genetics Laboratory Department of BiologyCenter of Biological SciencesFederal University of ParáBelémPABrazil Genetics Division Department of Morphology Federal University of Sao PauloSao PauloSPBrazil Department of Pathology and Surgery Service Federal University of ParáBelémPABrazil Joao de Barros Barreto University Hospital Federal University of ParáBelémPABrazil Molecular Genetics Laboratory Department of Pathology Medical SchoolFederal University of CearáFortalezaCEBrazil

AIM: To investigate chromosome 8 numerical aberra- tions, C-MYC oncogene alterations and its expression in gastric cancer and to correlate these findings with histo- pathological characteristics of gastric tumors. METHODS: Specimens were collected surgically from seven patients with gastric adenocarcinomas. Immu- nostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) for C-MYC gene and chromosome 8 centromere were performed. RESULTS: All the cases showed chromosome 8 aneu- ploidy and C-MYC amplification, in both the diffuse and intestinal histopathological types of Lauren. No significant difference (P < 0.05) was observed between the level ofchromosome 8 ploidy and the site, stage or histological type of the adenocarcinomas. C-MYC high amplification, like homogeneously stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Structural rearrangement of C-MYC, like translocation, was observed only in the diffuse type. Regarding C-MYC gene, a significant difference (P < 0.05) was observed between the two histological types. The C-MYC protein was expressed in all the studied cases. In the intestinal- type the C-MYC immunoreactivity was localized only in the nucleus and in the diffuse type in the nucleus and cytoplasm. CONCLUSION: Distinct patterns of alterations between intestinal and diffuse types of gastric tumors support the hypothesis that these types follow different genetic path- ways.

关键词： Chromosome 8 aneuploidy C-MYC amplification Immunostaining Gastric adenocarcinoma

Identification of IL11RA and MELK amplification in gastric cancer by comprehensive genomic profiling of gastric cancer cell lines

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World Journal of Gastroenterology 2016年第43期22卷 9506-9514页

作者： Danielle Queiroz Calcagno Sylvia Santomi Takeno Carolina Oliveira Gigek Mariana Ferreira Leal Fernanda Wisnieski elizabeth suchi chen Taíssa Maíra Thomaz Araújo Eleonidas Moura Lima Maria Isabel Melaragno Samia Demachki Paulo Pimentel Assumpcao Rommel Rodriguez Burbano Marília Cardoso Smith Disciplina de Genética Departamento de Morfologia e GenéticaUniversidade Federal de Sao Paulo Núcleo de Pesquisas em Oncologia Hospital Universitário Joao de Barros Barreto Departamento de Biologia Molecular Universidade Federal da Paraíba Disciplina de Gastroenterologia Cirúrgica Universidade Federal de Sao Paulo Departamento de Ortopedia e Traumatologia Universidade Federal de Sao Paulo

AIM To identify common copy number alterations on gastric cancer cell *** Four gastric cancer cell lines(ACP02, ACP03, AGP01 and PG100) underwent chromosomal comparative genome hybridization and array comparative genome hybridization. We also confirmed the results by fluorescence in situ hybridization analysis using the bacterial artificial chromosome clone and quantitative real time PCR *** The amplification of 9p13.3 was detected in all cell lines by both methodologies. An increase in the copy number of 9p13.3 was also confirmed by fluorescence in situ hybridization analysis. Moreover, the interleukin 11 receptor alpha(IL11RA) and maternal embryonic leucine zipper kinase(MELK) genes, which are present in the 9p13.3 amplicon, revealed gains of the MELK gene in all the cell lines studied. Additionally, a gain in the copy number of IL11 RA and MELK was observed in 19.1%(13/68) and 55.9%(38/68) of primary gastric adenocarcinoma samples, respectively. CONCLUSION The characterization of a small gain region at 9p13.3 in gastric cancer cell lines and primary gastric adenocarcinoma samples has revealed MELK as a candidate target gene that is possibly related to the development of gastric cancer.

关键词： IL11RA Gastric cancer Genomic profiling MELK 9p13.3

Reference genes for quantitative RT-PCR data in gastric tissues and cell lines

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World Journal of Gastroenterology 2013年第41期19卷 7121-7128页

作者： Fernanda Wisnieski Danielle Queiroz Calcagno Mariana Ferreira Leal Leonardo Caires dos Santos Carolina de Oliveira Gigek elizabeth suchi chen Thaís Brilhante Pontes Paulo Pimentel Assumpo Mnica Barauna de Assumpo Smia Demachki Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith Disciplina de Genética Departamento de Morfologia e GenéticaUniversidade Federal de So PauloSo Paulo 04023900Brazil Departamento de Ortopedia e Traumatologia Universidade Federal de So PauloSo Paulo 04038031Brazil Núcleo de Pesquisa em Oncologia Hospital Joo de Barros BarretoUniversidade Federal do ParáBelém 60673000Brazil Laboratório de Citogenética Humana Instituto de Ciências BiológicasUniversidade Federal do ParáBelém 66075110Brazil

AIM:To evaluate the suitability of reference genes in gastric tissue samples and cell ***:The suitability of genes ACTB,B2M,GAPDH,RPL29,and 18S rRNA was assessed in21 matched pairs of neoplastic and adjacent nonneoplastic gastric tissues from patients with gastric adenocarcinoma,27 normal gastric tissues from patients without cancer,and 4 cell lines using reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR).The ranking of the best single and combination of reference genes was determined by NormFinder,geNorm,BestKeeper,and *** addition,GenEx software was used to determine the optimal number of reference *** validate the results,the mRNA expression of a target gene,DNMT1,was quantified using the different reference gene combinations suggested by the various software packages for ***:ACTB was the best reference gene for all gastric tissues,cell lines and all gastric tissues plus cell ***+B2M or ACTB+B2M was the best combination of reference genes for all the gastric *** the other hand,ACTB+B2M was the best combination for all the cell lines tested and was also the best combination for analyses involving all the gastric tissues plus cell *** to the GenEx software,2 or 3 genes were the optimal number of references genes for all the gastric *** relative quantification of DNMT1 showed similar patterns when normalized by each combination of reference *** level of expression of DNMT1 in neoplastic,adjacent non-neoplastic and normal gastric tissues did not differ when these samples were normalized using GAPDH+B2M(P=0.32),ACTB+B2M(P=0.61),or GAPDH+B2M+ACTB(P=0.44).CONCLUSION:GAPDH+B2M or ACTB+B2M is the best combination of reference gene for all the gastric tissues,and ACTB+B2M is the best combination for the cell lines tested.

关键词： Gastric cancer Reference gene Normalization Gene expression Quantitative real-time polymerase chain reaction

Prevalence of Helicobacter pylori vacA,cagA,dupA and oipA Genotypes in Patients with Gastric Disease

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Advances in Microbiology 2017年第1期7卷 1-9页

作者： Mayara Luciana Sallas Jessica Lima Melchiades Luanna Munhoz Zabaglia Juliana Ribeiro do Prado Moreno Wilson Aparecido Orcini elizabeth suchi chen Marilia de Arruda Cardoso Smith Spencer Luiz Marques Payao Lucas Trevizani Rasmussen Sagrado Coracao University(USC) BauruBrazil Federal University of Sao Paulo(UNIFESP) Sao PauloBrazil

Gastric diseases such as chronic gastritis and gastric cancer are most commonly caused by virulence factors of Helicobacter pylori (H. pylori), such as the vacA, cagA, dupA and oipA genes. Therefore, this study investigated the prevalence and the combination of these virulence factors from patients with gastric diseases. The endoscopic biopsies were obtained from 516 patients with gastric symptoms, 101 of which were from patients with normal gastric tissue, 365 of which were from patients with chronic gastritis, and 50 of which were from patients with gastric cancer. H. pylori and the virulence factors were detected by PCR. The oipA gene exhibited an increased risk for chronic gastritis (p = 0.0296), and the vacA gene demonstrated a risk for gastric cancer from chronic gastritis (p = 0.0002). Based on the combination of the virulence factors, cagA, vacA, dupA and oipA genes exhibited a high prevalence in patients with chronic gastritis and gastric cancer. The cagA+/dupA+ genotype demonstrated a significant correlation in patients with normal gastric mucosa (p = 0.0278). In the chronic gastritis group, a significant association was observed between the cagA+ and the vacA s1m1 genotypes (p cagA+/dupA+ genotypes (p = 0.0183), the dupA+/oipA+ genotypes (p cagA, vacA, dupA, and oipA genes, which contributed to the risk of developing gastroduodenal diseases. Furthermore, this is the first study to reveal a high prevalence of the oipA gene in H. pylori isolates in Brazil.

关键词： Helicobacter pylori Virulence Factors cagA dupA oipA vacA

Melatonin lowers edema after spinal cord injury

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Neural Regeneration Research 2014年第24期9卷 2205-2210页

作者： cheng Li Xiao chen suchi Qiao Xinwei Liu Chang Liu Degang Zhu Jiacan Su Zhiwei Wang Department of Orthopedic Surgery Changhai Hospital the Second Military Medical University Laboratory of Severe and War-Related Trauma Center General Hospital of Shenyang Military Area Command of Chinese PLA

Melatonin has been shown to diminish edema in rats. Melatonin can be used to treat spinal cord injury. This study presumed that melatonin could relieve spinal cord edema and examined how it might act. Our experiments found that melatonin （100 mg/kg, i.p.） could reduce the water content of the spinal cord, and suppress the expression of aquaporin-4 and glial fibrillary acidic protein after spinal cord injury. This suggests that the mechanism by which melatonin alleviates the damage to the spinal cord by edema might be related to the expression of aquaporin-4 and glial fibrillary acidic protein.

关键词： nerve regeneration spinal cord injury melatonin edema aquaporin glial fibrillary acidic protein immunohistochemistry western blot assay neural regeneration

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应用CRISP清单,提升我国全科医学和基本医疗研究报告的质量

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中国全科医学 2025年第4期28卷 393-401页

作者：杨辉汪洋陈庆奇刘晨曦顾杰赵洋秦江梅曹新阳 elizabeth Sturgiss William R.Phillips 许岩丽 Monash大学公共卫生与预防医学学院澳大利亚墨尔本市VIC 3168 上海市全科医学与社区卫生发展研究中心 200090 同济大学附属杨浦医院全科医学科上海市200090 香港大学家庭医学及基层医疗学系中国香港特别行政区999077 华中科技大学同济医学院医药卫生管理学院湖北省武汉市430000 复旦大学附属中山医院全科医学科上海市200032 复旦大学附属中山医院国际医疗部上海市200032 新南威尔士大学乔治全球健康研究院澳大利亚悉尼2050 墨尔本大学人口与全球健康学院澳大利亚墨尔本3010 国家卫生健康委卫生发展研究中心北京市100044 《中国全科医学》杂志社有限公司北京市100053 School of Primary and Allied Health Care Monash UniversityMelbourneVictoriaAustraliaVIC 3168 University of Washington SeattleWashington98195

基本医疗(初级保健)研究报告内容的条目共识(CRISP)工作组于2023年提出了CRISP清单,以支持改善上述领域研究报告的质量、实用性和传播性。《中国全科医学》英文版的学术编辑团队对CRISP清单进行了中文翻译,并通过这篇述评,在回顾我国全... 详细信息

基本医疗(初级保健)研究报告内容的条目共识(CRISP)工作组于2023年提出了CRISP清单,以支持改善上述领域研究报告的质量、实用性和传播性。《中国全科医学》英文版的学术编辑团队对CRISP清单进行了中文翻译,并通过这篇述评,在回顾我国全科医学和基本医疗研究的内涵和发展现状的基础上,归纳了该领域的科研报告中目前存在的主要问题,如“以作者为中心”“过度重视塑造权威性”“盲目生搬国际经验”“缺乏对方法学的透明报告”“在论文中压入大量冗繁信息”以及“遮蔽关键信息”等。最后,编辑团队推荐和鼓励中国的全科医学和基本医疗服务研究的作者、编辑和学术评议专家尝试使用CRISP清单,在改善中国全科医学和基本医疗研究报告质量的同时,进一步完善该工具在学科发展研究、临床研究、卫生服务研究、卫生政策研究、教育和培训研究以及社区流行病学研究等亚领域中的应用。

关键词：全科医学基本医疗研究报告内容共识

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Can a multistage approach improve individual tree mortality predictions across the complex mixed-species and managed forests of eastern North America?

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Forest Ecosystems 2023年第1期10卷 21-30页

作者： Cen chen John Kershaw Jr Aaron Weiskittel elizabeth McGarrigle Center for Research on Sustainable Forests University of MaineOronoME04469USA Faculty of Forestry&Environmental Management University of New BrunswickFrederictonNBE3B 5A3Canada Nova Scotia Department of Natural Resources HalifaxNSB3J 3M8Canada

Tree mortality plays a fundamental role in the dynamics of forest ecosystems,yet it is one of the most difficult phenomena to accurately *** modeling strategies have been developed to improve individual tree mortality *** less explored strategy is the use of a multistage modeling *** improvements from this approach have remained largely *** this study,we developed a novel multistage approach and compared its performance in individual tree mortality predictions with a more conventional approach using an identical individual tree mortality model *** permanent plot data(n=9442)covering the Acadian Region of North America and over multiple decades(1965–2014)were used in this *** results indicated that the model behavior with the multistage approach better depicted the observed mortality and showed a notable improvement over the conventional *** difference between the observed and predicted numbers of dead trees using the multistage approach was much smaller when compared with the conventional *** addition,tree survival probabilities predicted by the multistage approach generally were not significantly different from the observations,whereas the conventional approach consistently underestimated mortality across species and overestimated tree survival probabilities over the large range of DBH in the *** new multistage approach also predictions of zero mortality in individual plots,a result not possible in conventional ***,the new approach was more tolerant of modeling errors because it based estimates on ranked tree mortality rather than error-prone predicted ***,this new multistage approach deserves to be considered and tested in future studies.

关键词： Tree mortality modeling Mortality disaggregation Mixed effect model Annualization Mixed forests

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Continuous roll-to-roll patterning of three-dimensional periodic nanostructures

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Microsystems & Nanoengineering 2020年第1期6卷 1067-1077页

作者： I-Te chen elizabeth Schappell Xiaolong Zhang Chih-Hao Chang Department of Mechanical and Aerospace Engineering North Carolina State UniversityRaleighNC 27695USA Walker Department of Mechanical Engineering University of Texas at AustinAustinTX 78712USA

In this work,we introduce a roll-to-roll system that can continuously print three-dimensional(3D)periodic nanostructures over large *** approach is based on Langmuir-Blodgett assembly of colloidal nanospheres,which diffract normal incident light to create a complex intensity pattern for near-field *** geometry of the 3D nanostructure is defined by the Talbot effect and can be precisely designed by tuning the ratio of the nanosphere diameter to the exposure *** this system,we have demonstrated patterning of 3D photonic crystals with a 500 nm period on a 50×200 mm^(2) flexible substrate,with a system throughput of 3mm/*** patterning yield is quantitatively analyzed by an automated electron beam inspection method,demonstrating long-term repeatability of an up to 88% yield over a 4-month *** inspection method can also be employed to examine pattern uniformity,achieving an average yield of up to 78.6% over full substrate *** proposed patterning method is highly versatile and scalable as a nanomanufacturing platform and can find application in nanophotonics,nanoarchitected materials,and multifunctional nanostructures.

关键词： tuning lithography periodic

Utilizing bimetallic catalysts to mitigate coke formation in dry reforming of methane

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Journal of Energy Chemistry 2022年第5期31卷 124-142页

作者： Jaylin Sasson Bitters Tina He elizabeth Nestler Sanjaya D.Senanayake Jingguang G.chen cheng Zhang College of Liberal Arts and Sciences Long Island University(Post)BrookvilleNY 11548United States Department of Molecular Biosciences University of Texas at AustinAustinTX 78712United States Chemistry Division Brookhaven National LaboratoryUptonNY 11973United States Department of Chemical Engineering Columbia UniversityNew YorkNY 10027United States

Dry reforming of methane(DRM) involves the conversion of carbon dioxide(CO_(2)) and methane(CH_(4)) into syngas(a mixture of hydrogen, H_(2), and carbon monoxide, CO), which can then be used to produce a wide range of products by means of Fischer–Tropsch synthesis. DRM has gained much attention as a means of mitigating damage from anthropogenic greenhouse gas(GHGs) emissions to the environment and instead utilizing these gases as precursors for value-added chemicals or to synthesize sustainable fuels and chemicals. Carbon deposition or coke formation, a primary cause of catalyst deactivation, has proven to be a major challenge in the development of DRM catalysts. The use of nickel-and cobalt-based catalysts has been extensively explored for DRM for their high activity and low cost but suffer from poor stability due to coke formation that has hindered their commercialization. Numerous articles have reviewed the various aspects of catalyst deactivation and strategies for mitigation, but few has focused on the benefit of bimetallic catalysts for mitigating coke formation. Bimetallic catalysts, often improve the catalytic stability over their monometallic counterparts due to synergistic effects resulting from two metal-tometal interactions. This review will cover DRM literature for various bimetallic catalyst systems, including the effect of supports and promoters, on the mitigation of carbonaceous deactivation.

关键词： Dry reforming of methane Carbon dioxide Bimetallic catalysts Coke formation Catalyst stability

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