检索结果-南通市图书馆

Protein & Cell 2010年第7期1卷 621-626页

作者： Hong Gao Ying Zhuo elizabeth Ashforth Lixin Zhang CAS Key Laboratory of Pathogenic Microbiology&Immunology Institute of MicrobiologyChinese Academy of Sciences(CAS)Beijing 100101China Graduate University of Chinese Academy of Sciences Beijing 100049China

Synthetic biology aims to design and build new biological systems with desirable properties,providing the foundation for the biosynthesis of secondary *** most prominent representation of synthetic biology has been used in microbial engineering by recombinant DNA ***,there are advantages of using a deleted host,and therefore an increasing number of biotechnology studies follow similar strategies to dissect cellular networks and construct genomereduced *** review will give an overview of the strategies used for constructing and engineering reduced-genome factories by synthetic biology to improve production of secondary metabolites.

关键词： synthetic biology reduced-genome secondary metabolite

Orotic Acid, More Than Just an Intermediate of Pyrimidine de novo Synthesis

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2015年第5期42卷 207-219页

作者： Monika L?ffler elizabeth A.Carrey Elke Zameitat Institute of Physiological Chemistry Faculty of Medicine Philipps University Marburg UCL Institute of Child Health University College London

It is timely to consider the many facets of the small molecule orotic acid （OA）, which is well-known as an essential intermediate of pyrimidine de novo synthesis. In addition, it can be taken up by erythrocytes and hepatocytes for conversion into uridine and for use in the pyrimidine recycling pathway. We discuss the link between dietary orotate and fatty liver in rats, and the potential for the alleviation of neonatal hyperbilirubinaemia. We address the development of orotate derivatives for application as anti-pyrimidine drugs, and of com- plexes with metal ions and organic cations to assist therapies of metabolic syndromes. Recent genetic data link human Miller syndrome to defects in the dihydroorotate dehydrogenase （DHODH） gene, hence to depleted orotate production. Another defect in pyrimidine biosynthesis, the orotic aciduria arising in humans and cattle with a deficiency of UMP synthase （UMPS）, has different symptoms. More recent work leads us to conclude that OA may have a role in regulating gene transcription.

关键词： Orotic acid Pyrimidines Orotic aciduria Miller syndrome Gene defects

Protocoled thrombolytic therapy for frostbite improves phalangeal salvage rates

在线全文

学校读者我要写书评

暂无评论

Burns & Trauma 2020年第1期8卷 338-346页

作者： Rosemary elizabeth Paine elizabeth Noel Turner Daniel Kloda Carolyne Falank Bruce Chung Damien Wilson Carter Department of Surgery Maine Medical Center22 Bramhall StPortlandMaine 04102USA Department of Radiology Maine Medical Center22 Bramhall StPortlandMaine 04102USA

Background:Frostbite is a cold injury that has the potential to cause considerable morbidity and long-term *** the complexity of these patients,diagnostic and treatment practices lack *** therapy has emerged as a promising treatment modality,demonstrating impressive digit salvage *** review our experience with thrombolytic therapy for severe upper extremity ***:Retrospective data on all frostbite patients evaluated at our institution from December 2017 to March 2018 was collected.A subgroup of patients with severe frostbite treated with intraarterial thrombolytic therapy(IATT)were ***:Of the 17 frostbite patients treated at our institution,14(82%)were male and the median age was 31(range:19–73).Substance misuse was involved in a majority of the cases(58.8%).Five(29.4%)patients with severe frostbite met inclusion criteria for IATT and the remaining patients were treated *** demonstrated a 74.5%improvement in perfusion after tissue plasminogen activator *** comparing phalanges at risk on initial angiography to phalanges undergoing amputation,the phalangeal salvage rate was 83.3%and the digit salvage rate was 80%.Complications associated with IATT included groin hematoma,pseudoaneurysm and retroperitoneal ***:Thrombolytic therapy has the potential to greatly improve limb salvage and functional recovery after severe frostbite when treated at an institution that can offer comprehensive,protocoled thrombolytic therapy.A multi-center prospective study is warranted to elucidate the optimal treatment strategy in severe frostbite.

关键词： Frostbite Tissue plasminogen activator Amputation Digit salvage Thrombolysis

The Perpetual Crisis of Journalism： Cable and Digital Revolutions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fudan Journal of the Humanities and Social Sciences 2015年第1期8卷 49-59页

作者： elizabeth Butler Breese Panorama Education Cambridge MA USA

There pervasive sense today that journalism is in crisis is an expression of the perpetual crisis of American journalism. The crisis of American journalism is articulated through a narrative of decline that warns that the future of journalism will be worse than the past. Through discourse analysis of the introduction of new technologies and a new news anchor in journalism, this study shows that the dis- course of crisis and grave doubts about the future of professional journalism are not new. Today and in previous eras, the past seems to represent the high mark of professional journalism in the USA, and the future of the news seems exceptionally uncertain. These perennial claims of the decline of journalism serve to reinforce the sacred ideals and standards of journalism.

关键词： News · Journalism · Crisis · Cultural sociology · Cable news · Twitter

Plant Growth Promoting Fungal and Bacterial Endophytes of Tall Fescue: A Review

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Grass Research 2022年第1期2卷 14-19页

作者： elizabeth Lewis Roberts Southern Connecticut State University New Haven Connecticut06515USA

Plant growth promoting bacteria(PGPB)directly and indirectly influence the fitness of their host *** much of the work on plant growth promoting bacteria is from various plant rhizospheres,less is known about the strains that inhabit the internal spaces of plant ***,Clavicipitalean fungal endophytes of cool season grasses have been intensely investigated for the past few *** plant growth promoting bacteria,the fungal endophytes also promote the health of their *** paper highlights the current knowledge of plant growth promoting bacterial and fungal endophytes of tall fescue(Lolium arundinaceum=Schedonorus arundinaceaus=Festuca arundinacea)which protect the plant from both abiotic and biotic *** of the work presented indicates just how little is known about the inter-relationships between the multiple endophytic microbial colonizers of turf grasses.

关键词： donor intense spaces

Quantification of changes in zero valent iron morphology using X-ray computed tomography

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2013年第11期25卷 2344-2351页

作者： Ping Luo elizabeth H.Bailey Sacha J.Mooney Division of Agricultural and Environmental Sciences School of Biosciences University of Nottingham Sutton Bonington CampusSutton Bonington

Morphological changes within the porous architecture of laboratory scale zero valent iron （ZVI） permeable reactive barriers （PRBs）, after exposure to different groundwater conditions, have been quantified experimentally for different ZVI/sand ratios （10%, 50% and 100%, W/W） with the aim of inferring porosity changes in field barriers. Column studies were conducted to simulate interaction with different water chemistries, a synthetic groundwater, acidic drainage and deionised （DI） water as control. Morphological changes, in terms of pore size and distribution, were measured using X-ray computed tomography （CT）. CT image analysis revealed significant morphological changes in columns treated with different water chemistries. For example, 100% ZVI （W/W） columns had a higher frequency of small pores （0.6 mm） was observed in ZVI grains reacted with typical groundwater, resulting in a porosity of 27%, compared to 32% when exposed to DI water. In comparison, ZVI grains treated with the acidic drainage had higher porosity （44%） and larger average pore size （2.8 mm）. 10% ZVI PRB barrier material had the highest mean porosity （56%） after exposure to any water chemistry whilst 100% ZVI （W/W） columns always had the lowest （34%） with the 50% ZVI （W/W） in between （40%）. These results agree with previously published PRB field data and simultaneously conducted geochemical monitoring and mass balance calculation, indicating that both the geochemical and hydraulic environment of the PRB play an important role in determining barrier lifespan. This study suggests that X-ray CT image analysis is a powerful tool for studying the detailed inter pores between ZVI grains within PRBs.

关键词： porosity change X-ray computer tomography mass balance permeable reactive barrier zero valent iron acidic drainage typical groundwater pore-clogging

Can a multistage approach improve individual tree mortality predictions across the complex mixed-species and managed forests of eastern North America?

在线全文

学校读者我要写书评

暂无评论

Forest Ecosystems 2023年第1期10卷 21-30页

作者： Cen Chen John Kershaw Jr Aaron Weiskittel elizabeth McGarrigle Center for Research on Sustainable Forests University of MaineOronoME04469USA Faculty of Forestry&Environmental Management University of New BrunswickFrederictonNBE3B 5A3Canada Nova Scotia Department of Natural Resources HalifaxNSB3J 3M8Canada

Tree mortality plays a fundamental role in the dynamics of forest ecosystems,yet it is one of the most difficult phenomena to accurately *** modeling strategies have been developed to improve individual tree mortality *** less explored strategy is the use of a multistage modeling *** improvements from this approach have remained largely *** this study,we developed a novel multistage approach and compared its performance in individual tree mortality predictions with a more conventional approach using an identical individual tree mortality model *** permanent plot data(n=9442)covering the Acadian Region of North America and over multiple decades(1965–2014)were used in this *** results indicated that the model behavior with the multistage approach better depicted the observed mortality and showed a notable improvement over the conventional *** difference between the observed and predicted numbers of dead trees using the multistage approach was much smaller when compared with the conventional *** addition,tree survival probabilities predicted by the multistage approach generally were not significantly different from the observations,whereas the conventional approach consistently underestimated mortality across species and overestimated tree survival probabilities over the large range of DBH in the *** new multistage approach also predictions of zero mortality in individual plots,a result not possible in conventional ***,the new approach was more tolerant of modeling errors because it based estimates on ranked tree mortality rather than error-prone predicted ***,this new multistage approach deserves to be considered and tested in future studies.

关键词： Tree mortality modeling Mortality disaggregation Mixed effect model Annualization Mixed forests

Operable gastro-oesophageal junctional adenocarcinoma: Where to next?

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2014年第6期6卷 145-155页

作者： elizabeth C Smyth David Cunningham Department of Gastrointestinal Oncology Royal Marsden Hospital

Oesophageal junctional adenocarcinoma is a challeng-ing and increasingly common disease. Optimisation ofpre-operative staging and consolidation of surgery inlarge volume centres have improved outcomes, howev-er the preferred adjunctive treatment approach remainsa matter of debate. This review examines the benefitsof neoadjuvant, peri-operative, and post-operative che-motherapy and chemoradiotherapy in this setting in anattempt to reach an evidence based conclusion. Recentfindings relating to the molecular characterisation ofoesophagogastric cancer and their impact on therapeu-tics are explored, in addition to the potential benefitsof fluoro-deoxyglucose positron emission tomography(FDG-PET) directed therapy. Finally, efforts to decreasethe incidence of junctional adenocarcinoma using earlyintervention in Barrett's oesophagus are discussed,including the roles of screening, endoscopic mucosalresection, ablative therapies and chemoprevention.

关键词： Oesophageal adenocarcinoma Junctional adenocarcinoma Gastric adenocarcinoma Peri-operative chemotherapy Pre-operative chemoradiotherapy Molecular profiling Fluoro-deoxyglucose-positron emission tomography Barrett's oesophagus Chemoprevention

Emerging molecular targets and therapy for cholangiocarcinoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2017年第7期9卷 268-280页

作者： Hamzeh Kayhanian elizabeth C Smyth Chiara Braconi Department of Gastrointestinal Oncology Royal Marsden Hospital London and Surrey SM2 5PT Institute of Cancer Research London and Surrey SM2 5PT

Cholangiocarcinoma(CCA) is a rare cancer arising from the biliary tree with a poor prognosis and limited therapeutic options. Recent large scale molecular characterisation studies have identified recurrent genetic alterations in CCA which may be amenable to therapeutic targeting. In this review we explore the genomic landscape of CCA and examine results from trials of molecularly targeted agents and immunotherapy in this disease. Challenges in CCA diagnosis, treatment and trial design are discussed and we reflect on future directions which may lead to improved outcomes for CCA patients.

关键词： Cholangiocarcinoma Biliary tract cancer Targeted therapy immunotherapy Mutation Molecular Microenvironment Stroma MiRNA

Aging gracefully: social engagement joins exercise and enrichment as a key lifestyle factor in resistance to age-related cognitive decline

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2019年第1期14卷 39-42页

作者： Tyler J.Dause elizabeth D.Kirby Department of Psychology The Ohio State University Department of Neuroscience The Ohio State University Chronic Brain Injury Program The Ohio State University

Cognitive impairment is a consequence of the normal aging process that effects many species, including humans and rodent models. Decline in hippocampal memory function is especially prominent with age and often reduces quality of life. As the aging population expands, the need for interventional strategies to prevent cognitive decline has become more pressing. Fortunately, several major lifestyle factors have proven effective at combating hippocampal aging, the most well-known of which are environmental enrichment and exercise. While the evidence supporting the beneficial nature of these factors is substantial, a less well-understood factor may also contribute to healthy cognitive aging: social engagement. We review the evidence supporting the role of social engagement in preserving hippocampal function in old age. In elderly humans, high levels of social engagement correlate with better hippocampal function, yet there is a dearth of work to indicate a causative role. Existing rodent literature is also limited but has begun to provide causative evidence and establish candidate mechanisms. Summed together, while many unanswered questions remain, it is clear that social engagement is a viable lifestyle factor for preserving cognitive function in old age. Social integration across the lifespan warrants more investigation and more appreciation when designing living circumstances for the elderly.

关键词： aging age-related cognitive decline social engagement hippocampus cognitive enrichment exercise memory neurogenesis