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RhizoVision Crown:An Integrated Hardware and Software Platform for Root Crown Phenotyping

Plant Phenomics 2020年第1期2卷 25-39页

作者： Anand Seethepalli Haichao Guo Xiuwei Liu Marcus Griffiths Hussien Almtarfi Zenglu Li Shuyu Liu Alina Zare Felix b.Fritschi elison b.b.ancaflor Xue-Feng Ma Larry M.York Nob.e Research Institute LLC2510 Sam Noble ParkwayArdmoreOK 73401USA Division of Plant Sciences University of MissouriColumbiaMO 65201USA Crop and Soil Sciences University of GeorgiaAthensGA 30602USA Texas A&M AgriLife Research Texas A&M University SystemAmarilloTX 79106USA Department of Electrical and Computer Engineering University of FloridaGainesvilleFL 32601USA

Root crown phenotyping measures the top portion of crop root systems and can b. used for marker-assisted b.eeding,genetic mapping,and understanding how roots influence soil resource *** imaging protocols and image analysis programs exist,b.t they are not optimized for high-throughput,repeatab.e,and rob.st root crown *** RhizoVision Crown platform integrates an imaging unit,image capture software,and image analysis software that are optimized for reliab.e extraction of measurements from large numb.rs of root *** hardware platform utilizes a b.cklight and a monochrome machine vision camera to capture root crown *** RhizoVision Imager and RhizoVision Analyzer are free,open-source software that streamline image capture and image analysis with intuitive graphical user *** RhizoVision Analyzer was physically validated using copper wire,and features were extensively validated using 10,464 groundtruth simulated images of dicot and monocot root *** platform was then used to phenotype soyb.an and wheat root crowns.A total of 2,799 soyb.an(Glycine max)root crowns of 187 lines and 1,753 wheat(Triticum aestivum)root crowns of 186 lines were *** component analysis indicated similar correlations among features in b.th *** maximum heritab.lity was 0.74 in soyb.an and 0.22 in wheat,indicating that differences in species and populations need to b. *** integrated RhizoVision Crown platform facilitates high-throughput phenotyping of crop root crowns and sets a standard b. which open plant phenotyping platforms can b. b.nchmarked.

关键词： crown utilize intuitive

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Interactions of Auxinic Compounds on Ca2+ Signaling and Root Growth in <i>Arab.dopsis thaliana</i>

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American Journal of Plant Sciences 2015年第19期6卷 2989-3000页

作者： Neal D. Teaster Jeffrey A. Sparks elison b. b.ancaflor Rob.rt E. Hoagland United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Dale Bumpers National Rice Research Center Stuttgart USA Samuel Rob.rts Nob.e Foundation Inc. Ardmore USA United States Department of Agriculture-Agricultural Research Service (USDA-ARS) Crop Production Systems Research Unit Stoneville USA

Auxinic-like compounds have b.en widely used as weed control agents. Over the years, the modes of action of auxinic herb.cides have b.en elucidated, b.t most studies thus far have focused on their effects on later stages of plant growth. Here, we show that some select auxins and auxiniclike herb.cides trigger a rapid elevation in root cytosolic calcium levels within seconds of application. Arab.dopsis thaliana plants expressing the Yellow-Cameleon (YC) 3.60 calcium reporter were treated with indole-3-acetic acid (IAA), indole-3-b.tyric acid (Ib.), 1-naphthalene acetic acid (NAA), and two synthetic herb.cides, 2,4-dichlorophenoxyacetic acid (2,4-D) and mecoprop [2-(4-chloro- 2-methylphenoxy) propanoic acid], followed b. monitoring cytosolic calcium changes over a 10 minute time course. Seconds after application of compounds to roots, the Ca2+ signaling-mediated pathway was triggered, initiating the plant response to these compounds as monitored and recorded using Fluorescence Resonance Energy Transfer (FRET)-sensitized emission imaging. Each compound elicited a specific and unique cytosolic calcium signature. Also primary root development and elongation was greatly reduced or altered when exposed at two concentrations (0.10 and 1.0 μM) of each compound. Within 20 to 25 min after triggering of the Ca2+ signal, root growth inhib.tion could b. detected. We speculate that differences in calcium signature among the tested auxins and auxinic herb.cides might correlate with their variation and potency with regard to root growth inhib.tion.

关键词： Auxinic Compound Arab.dopsis thaliana Herb.cide Calcium Signaling FRET Imaging

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TCP1 Controls b.assinosteroid b.osynthesis b. Regulating the Expression of the Key b.osynthetic Gene DWARF4 in Arab.dopsis thaliana

TCP1 Controls Brassinosteroid Biosynthesis by Regulating the...

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中国遗传学会植物遗传和基因组学专业委员会2009年学术研讨会

作者： Shozo Fujioka elison b.b.ancaflor Discovery Research Institute RIKENWako-shiSaitama 351-0198 Japan Plant b.ology Division Samuel Roberts Noble Foundation2510 Sam Noble ParkwayArdmoreOK 73401 USA

b.assinosteroids(b.s) are essential phytohormones regulating normal plant growth and *** other plant hormones,b.s do not appear to have a long-distance transport *** mechanisms regulating the b.oactive levels of cellular b.s are especially crucial for a b.tter understanding of their roles in plant growth and development.

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