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Liver Research 2017年第4期1卷 187-192页

作者： Cassandra A.Kersten elise n.sloey Eric Zhou Ying Peng Michael S.Torbenson Yi Guo Department of Biochemistry and Molecular Biology Mayo ClinicRochesterMNUSA Division of Gastroenterology and Liver Pathology Department of Laboratory Medicine and PathologyMayo ClinicRochesterMNUSA Division of Gastroenterology and Hepatology Mayo ClinicRochesterMNUSA

Fibrolamellar hepatocellular carcinoma(FLC)is a rare but aggressive liver cancer of children that occurs predominantly in teenagers without a history of liver *** resection remains the only therapeutic option,and the recurrence rate is extremely high(>50%within 3 years).A newly discovered chromosomal deletion that occurs in the majority of FLCs generates a novel kinase fusion between DnaJ heat shock protein family member B1(DnAJB1)and protein kinase cAMP-activated catalytic subunit alpha(PRKACA)(DnAJB1-PRKACA).Despite its high penetrance and apparent specificity for FLC,the oncogenic role of this fusion event remains *** this review article,we discuss the histology,presentation and diagnosis,current treatment,and roles of the DnAJB1-PRKACA as well as research models contributing to our understanding of this disease.

关键词： Fibrolamellar hepatocellular carcinoma(FLC) DnaJ heat shock protein family member B1 (DnAJB1) Protein kinase cAMP-activated catalytic subunit alpha(PRKACA)Pediatric cancer Liver cancer

Finding biomarkers of experience in animals

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Journal of Animal Science and Biotechnology 2024年第3期15卷 925-944页

作者： Sarah Babington Alan J.Tilbrook Shane K.Maloney Jill n.Fernandes Tamsyn M.Crowley Luoyang Ding Archa H.Fox Song Zhang elise A.Kho Daniel Cozzolino Timothy J.Mahony Dominique Blache School of Agriculture and Environment The University of Western AustraliaCrawleyWA 6009Australia Centre for Animal Science The Queensland Alliance for Agriculture and Food InnovationThe University of QueenslandSt LuciaQLD 4072Australia School of Veterinary Science The University of QueenslandGattonQLD 4343Australia School of Human Sciences The University of Western AustraliaCrawleyWA 6009Australia School of Medicine Deakin UniversityGeelongVIC 3217Australia Poultry Hub Australia University of New EnglandArmidaleNSW 2350Australia College of Animal Science and Technology Yangzhou UniversityYangzhou 225009China Centre for nutrition and Food Sciences The Queensland Alliance for Agriculture and Food InnovationThe University of QueenslandSt LuciaQLD 4072Australia

At a time when there is a growing public interest in animal welfare,it is critical to have objective means to assess the way that an animal experiences a *** is critical to ensure appropriate animal welfare *** behavioural,physiological,and neurobiological indicators that are used to assess animal welfare can verify the absence of extremely negative *** welfare is more than an absence of negative outcomes and an appropriate indicator should reflect the full spectrum of experience of an animal,from negative to *** this review,we draw from the knowledge of human biomedical science to propose a list of candidate biological markers(biomarkers)that should reflect the experiential state of non-human *** proposed biomarkers can be classified on their main function as endocrine,oxidative stress,non-coding molecular,and thermobiological *** also discuss practical challenges that must be addressed before any of these biomarkers can become useful to assess the experience of an animal in real-life.

关键词： Animal experience Animal welfare Biomarker Stress Welfare assessment

Base excision repair accessory factors in senescence avoidance and resistance to treatments

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Cancer Drug Resistance 2022年第3期5卷 703-720页

作者： elise Vickridge Camila C.F.Faraco Alain nepveu Goodman Cancer Institute McGill University1160 Pine avenue WestMontrealQuebec H3A 1A3Canada Departments of Biochemistry McGill University1160 Pine avenue WestMontrealQuebec H3A 1A3Canada Medicine McGill University1160 Pine avenue WestMontrealQuébec H3A 1A3Canada Oncology McGill University1160 Pine avenue WestMontrealQuébec H3A 1A3Canada

Cancer cells,in which the RAS and PI3K pathways are activated,produce high levels of reactive oxygen species(ROS),which cause oxidative DnA damage and ultimately cellular *** process has been documented in tissue culture,mouse models,and human pre-cancerous *** this context,cellular senescence functions as a tumour suppressor *** rare cancer cells,however,manage to adapt to avoid senescence and continue to *** well-documented mode of adaptation involves increased production of antioxidants often associated with inactivation of the KEAP1 tumour suppressor gene and the resulting upregulation of the nRF2 transcription *** this review,we detail an alternative mode of adaptation to oxidative DnA damage induced by ROS:the increased activity of the base excision repair(BER)pathway,achieved through the enhanced expression of BER enzymes and DnA repair accessory *** proteins,exemplified here by the CUT domain proteins CUX1,CUX2,and SATB1,stimulate the activity of BER *** ensued accelerated repair of oxidative DnA damage enables cancer cells to avoid senescence despite high ROS *** a by-product of this adaptation,these cancer cells exhibit increased resistance to genotoxic treatments including ionizing radiation,temozolomide,and ***,considering the intrinsic error rate associated with DnA repair and translesion synthesis,the elevated number of oxidative DnA lesions caused by high ROS leads to the accumulation of mutations in the cancer cell population,thereby contributing to tumour heterogeneity and eventually to the acquisition of resistance,a major obstacle to clinical treatment.

关键词： Base excision repair reactive oxygen species DnA repair accessory factor oxidative DnA damage resistance to treatment tumour heterogeneity acquisition of resistance

Oxidative dehydrogenation of propane under steady-state and transient regimes over alumina-supported catalysts prepared from mixed V_2W_4O_(19)^(4-) hexametalate precursors

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Journal of natural Gas Chemistry 2010年第2期19卷 123-133页

作者： Sébastien Paul elise Berrier Marcia Cristina Kaezer Frana Jean-Guillaume Eon Univ Lille nord de France F-59000 LilleFrance ECLille UCCSF-59650 Villeneuve d'AscqFrance CnRS UMR 8181F-59650 Villeneuve d'AscqFrance USTL UCCSF-59650 Villeneuve d'AscqFrance Instituto de Quimíca Universidade Federal do Rio de Janeiro-Cidade UniversitáriaIlha do Fundo21945-970 Rio de JaneiroBrazil

An integrated approach combining the development of an innovative catalyst and the research of a set of adequate operating conditions for the propane oxidative dehydrogenation （ODH） is *** experimental set-up,specially designed for steady-state and transient studies is *** preparation method,the characterization and the performances in steady-state and transient regimes of catalysts based on V2W4O194-Lindqvist isopolyanion used as a precursor and supported on alumina are *** influence of the preparation method of the catalyst and the role of water in the feed gas are more particularly discussed.

关键词： propane oxidative dehydrogenation (ODH) transient regime pulsed reactor Lindqvist isopolyanion in-situ Raman

Diversity patterns and palaeobiogeographical relationships of latest Devonian-Lower Carboniferous foraminifers from South China:What is global,what is local?

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Journal of Palaeogeography 2014年第1期3卷 35-59页

作者： Markus Aretz elise nardin Daniel Vachard Géosciences Environnement Toulouse (GET) Observatoire Midi Pyrénées Université de Toulouse CNRS IRD14 Avenue E. BelinF-31400 Toulouse France Université Lille 1 UMR 8217 Géosystèmes Batiment SN5Avenue Paul LangevinF-59655 Villeneuve d’Ascq cedex France

During latest Devonian and early Carboniferous times,calcareous foraminifers were abundant,widely distributed,and showed the most rapid rate of evolution in the shallow?sea *** factors,especially their fast phylogenetic changes,make them an essen?tial element in biostratigraphic schemes of this time ***,the distribution patterns of calcareous foraminifers depend on a series of biological and non?biological factors,such as population sizes,dispersion,oceanic currents and temperatures,and substrate types,which are not always well?controlled when interpreting spatial and temporal distribution *** this study,a dataset of calcareous foraminifers was compiled containing the tempo?ral distribution(biozone level)of 420 species belonging to 155 genera currently described from Strunian(latest Devonian)to basal Serpukhovian(Lower Carboniferous)key sections in southern China,and the presence of those species in eleven palaeobiogeographical *** comparison of the regional Chinese diversity curve,which has a bell?shaped form with a dou?ble peak in the Ivorian,to a global curve shows the influence of local and regional ***?mum values in the Chinese Strunian,basal Tournaisian and early late Visean can be explained by the small number of studied outcrops,unfavourable facies and depositional gaps in these stratigraphic intervals in South *** is especially obvious in the late Visean and Serpuk?hovian,which is a peak time of global *** fall observed at that time interval in southern China is easily explained by the fact that this time peried is far less intensively studied and thus fewer data are present in the *** opposite situation is seen around the TournaisianVisean ***,a peak is found in both regional and global curves,but that up to 87%of all known species are found in southern China seems to be unlikely,especially when the normal average value are 35%-40%.This anomalously high percentage is a cons

关键词： South China Devonian Carboniferous foraminifers diversity patterns statistical analyses palaeobiogeography

Refinement of saliva microRnA biomarkers for sports-related concussion

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Journal of Sport and Health Science 2023年第3期12卷 369-378页

作者： Steven D.Hicks Cayce Onks Raymond Y.Kim Kevin J.Zhen Jayson Loeffert Andrea C.Loeffert Robert P.Olympia Gregory Fedorchak Samantha DeVita Zofia Gagnon Callan McLoughlin Miguel M.Madeira Scott L.Zuckerman Timothy Lee Matthew Heller Chuck Monteith Thomas R.Campbell Christopher neville elise Fengler Michael n.Dretsch Department of Pediatrics College of MedicinePennsylvania State UniversityHersheyPA 17033USA Department of Orthopaedics and Rehabilitation College of MedicinePennsylvania State UniversityHersheyPA 17033USA Department of Family Medicine College of MedicinePennsylvania State UniversityHersheyPA 17033USA Department of Emergency Medicine College of MedicinePennsylvania State UniversityHersheyPA 17033USA Department of Research and Development Quadrant Biosciences Inc.SyracuseNY 13210USA Department of Biology Marist CollegePoughkeepsieNY 12601USA Sports Concussion Center College of MedicineVanderbilt UniversityNashvilleTN 37212USA Department of Family Medicine College of Osteopathic MedicineNew York Institute of TechnologyOld WestburyNY 11568USA Department of Athletic Training Colgate UniversityHamiltonNY 13346USA Department of Rehabilitation Sciences Old Dominion UniversityNorfolkVA 23529USA Department of Physical Therapy Education Orthopedicsand NeuroscienceCollege of MedicineSUNY Upstate Medical UniversitySyracuseNY 13210USA Department of Exercise Science Syracuse UniversitySyracuseNY 13210USA Department of Medical Research—West Walter Reed Army Institute of ResearchUS Army Joint Base Lewis-McChordHillhurstWA 98433USA

Background:Recognizing sport-related concussion(SRC)is challenging and relies heavily on subjective symptom *** objective,biological marker could improve recognition and understanding of *** is emerging evidence that salivary micro-ribonucleic acids(miRnAs)may serve as biomarkers of concussion;however,it remains unclear whether concussion-related miRnAs are impacted by *** sought to determine whether40 miRnAs previously implicated in concussion pathophysiology were affected by participation in a variety of contact and non-contact *** goal was to refine a miRnA-based tool capable of identifying athletes with SRC without the confounding effects of ***:This case-control study harmonized data from concussed and non-concussed athletes recruited across 10 *** of salivary miRnAs within 455 samples from 314 individuals were measured with RnA ***-subjects testing was used to identify and exclude miRnAs that changed with either(a)a single episode of exercise(166 samples from 83 individuals)or(b)season-long participation in contact sports(212 samples from 106 individuals).The miRnAs that were not impacted by exercise were interrogated for SRC diagnostic utility using logistic regression(172 samples from 75 concussed and 97 non-concussed individuals).Results:Two miRnAs(miR-532-5p and miR-182-5p)decreased(adjusted pngle episode of exercise,and 1 miRnA(miR-4510)increased only after contact sports ***-three miRnAs changed at the end of a contact sports *** of these miRnAs(miR-26b-3p and miR-29c-3p)were associated(R>0.50;adjusted pnumber of head impacts sustained in a single football *** the 15 miRnAs not confounded by exercise or season-long contact sports participation,11 demonstrated a significant difference(adjusted pn concussed and non-concussed participants,and 6 displayed moderate ability(area under curve>0.70)to identify concussion.A

关键词： Biomarker Contact sports Football RnA Traumatic brain injury

Long-term stability of gentamicin sulfate-ethylenediaminetetraacetic acid disodium salt (EDTA-na_2) solution for catheter locks

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Journal of Pharmaceutical Analysis 2018年第6期8卷 386-393页

作者： Anne-Sophie Fiolet elise Jandot Pauline Doucey Coralie Crétet Célia Brunel Christine Pivot Jean-Marc Ghigo Christophe Beloin David Lebeaux Fabrice Pirot Service Pharmaceutique Plateforme FRIPHARM Groupe Hospitalier Centre Edouard Herriot Hospices Civils de Lyon Unité de Génétique des Biofilms Département de Microbiologie Institut Pasteur Service de Microbiologie Unité Mobile de Microbiologie Clinique Assistance Publique-H?pitaux de Paris H?pital Européen Georges Pompidou Université Paris Descartes 12 rue de l'Ecole de Médecine Laboratoire de Recherche et Développement de Pharmacie Galénique Industrielle UMR 5305 Plateforme FRIPHARMF-69373 Faculté de Pharmacie Université Claude Bernard Lyon 1 8 avenue Rockefeller

A lock solution composed of gentamicin sulfate(5 mg/mL) and ethylenediaminetetraacetic acid disodium salt(EDTA-na2, 30 mg/mL) could fully eradicate in vivo bacterial biofilms in totally implantable venous access ports(TIVAP). In this study, fabrication, conditioning and sterilization processes of antimicrobial lock solution(ALS) were detailed and completed by a stability study. Stability of ALS was conducted for12 months in vial(25 °C 7 2 °C, 60% 7 5% relative humidity(RH), and at 40 °C 7 2 °C, RH 75% 7 5%)and for 24 h and 72 h in TIVAP(40 °C 7 2 °C, RH 75% 7 5%). A stability indicating HPLC assay with UV detection for simultaneous quantification of gentamicin sulfate and EDTA-na2 was developed. ALS was assayed by ion-pairing high performance liquid chromatography(HPLC) needing gentamicin derivatization, EDTA-na2 metallocomplexation of samples and gradient mobile phase. HPLC methods to separate four gentamicin components and EDTA-na2 were validated. Efficiency of sterility procedure and conditioning of ALS was confirmed by bacterial endotoxins and sterility tests. Physicochemical stability of ALS was determined by visual inspection, osmolality, pH, and sub-visible particle counting. Results confirmed that the stability of ALS in vials was maintained for 12 months and 24 h and 72 h in TIVAP.

关键词： Gentamicin-EDTA-na2 loaded antimicrobial lock solution Pharmaceutical compounding Stability indicating HPLC assay method Totally implantable venous access ports

Biochemical and Molecular Epidemiology of Cancer

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Biomedical and Environmental Sciences 1991年第1期4卷 73-92页

作者： HARUHIKO SUGIMURA AInSLEY WESTOn nEIL E.CAPORASO PETER G.SHIELDS elise D.BOWMAn ROBERT A.METCALF CURTIS C.HARRIS Laboratory of Human Carcinogenesis Laboratory of Human CarcinogenesisEnvironmental Epidemiology Branch National Cancer Institute National Institutes of HealthLaboratory of Human CarcinogenesisLaboratory of Human CarcinogenesisLaboratory of Human CarcinogenesisLaboratory of Human Carcinogenesis Rockville Maryland 20892

Examples of practical approaches to molecular epidemiology of human cancer are described. Biomarkers of carcinogen exposure or inherited host factors for cancer susceptibility are discussed. Major advances have been made in the detection of carcinogen-macromolecular adducts through the use of high performance liquid chromatography, immunoaffinity chromatography, the 32P-postlabeling assay, enzyme immunoassays, gas chromatography/mass spectroscopy and synchronous spectrophotofluorimetry. The polycyclic aromatic hydrocarbon- DnA adducts are the most extensively studied in this field and together with antibodies to these adducts found in human serum, they have become useful indicators of exposure to carcinogens. Assays for various kinds of alky 1-DnA adducts have also been developed and the presence of these adducts have been documented in human tissues. Carcinogen-protein adducts have proven to be useful molecular dosimeters of carcinogen exposure. For example, 4-aminobiphenyl hemoglobin adducts are highly correlated with exposure to tobacco smoke. The study of the molecular aspects of interindividual differences in the metabolism and activation of xenobiotics and other genetic markers [DnA-restriction fragment length polymorphisms (RFLPs), mutations, and functional loss of specific genes in carcinogenesis] is an emerging new field that is discussed in the context of genetic susceptibility to cancer. The cytochrome P450 phenotypes and acetylation phenotype are examples of genetic markers that indicate an individual's potential for metabolism of exogenous substances. Further, inherited genetic polymorphic markers, e.g., DnA-RFLPs at protooncogene loci (HRAS-1 and L-myc) have been examined in a case-control study of lung cancer. Data concerning mutations of protooncogenes (H-, K-, and n-RAS) and tumor suppressor genes (retinoblastoma and p53 genes) in various common cancers are providing evidence of multiple genetic lesions that occur during the multistage process of ca

关键词： gene DnA Biochemical and Molecular Epidemiology of Cancer

Multiscattering-enhanced absorbance to enable the sensitive analysis of extremely diluted biological samples: Determination of oxidative potential in exhaled air

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Medicine in novel Technology and Devices 2022年第2期14卷 62-66页

作者： Sami Goekce nicolas Concha-Lozano Jean-Jacques Sauvain Maud Hemmendinger Alejandro Portela elise Sergent Pascal Andujar Jean-Claude Pairon Pascal Wild Guillaume Suarez Center for Primary Care and Public Health Lausanne UniversityLausanneSwitzerland Forensic Toxicology and Chemistry Unit CURMLLausanne University HospitalGeneva University HospitalsSwitzerland International University of Catalonia Bioengineering Institute of TechnologyBarcelonaSpain CHI Creteil Service Pathologies Professionnelles et de L’EnvironnementCreteilFrance Universite Paris-Est Creteil Faculte de SanteInstitut Sante Travail Paris-Est CreteilFrance Inserm U955 Team 4CreteilFrance InRS Scientific ManagementVandoeuvre Les NancyFrance

Direct determination of the oxidative potential in exhaled air(OPEA)as a biological indicator of the redox balance status in airways requires an extremely sensitive sensing approach to counterbalance the high dilution of the exhaled air *** opposite to standard colorimetric assays based on Beer-Lambert Law,the use of simple multiscattering-enhanced absorbance(MEA)strategy results in an improved photonic sensing *** on this strategy and using the ferrous-xylenol orange assay,analytical performances allowing the detection of 3 pmol of H_(2)O_(2) could be *** comparative analysis of photometric configurations–standard vs MEA–highlights the large absorbance gain obtained for tiny H_(2)O_(2) amounts–from 0 to 30 pmol–whereas similar sensing trends are observed at higher concentration *** automated introduction of exhaled air sample into the reaction compartment via needle-impinger coupled with the MEA-based photonic cell enables ultrasensitive determination of OPEA in short time(n).A pilot study conducted on healthy subjects(n=22)and chronic obstructive pulmonary disease(COPD)patients(n=23)volunteers shows a significant increase of the OPEA for COPD versus controls and strong negative correlation between OPEA and spirometry parameters(Tiffeneau index:FEV1/FVC)for ex-/*** perspectives to assess inflammatory episodes in obstructive lung diseases are thus made possible using this sensing approach.

关键词： Absorbance enhancement Photonic device Oxidative potential Exhaled air COPD Pilot study

Effect of Equi-Strath<sup>®</sup>on Leucocyte Occurrence in the Haired Skin of Horses

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Agricultural Sciences 2018年第1期9卷 1-7页

作者： Danja D. Wiederkehr elise Jeannerat Paivi nussbaumer Dominik Burger Hendrika Anette van Dorland School of Agricultural Forest and Food Sciences Bern University of Applied Sciences Bern Switzerland Swiss Institute of Equine Medicine University of Bern and Agroscope Bern Switzerland

Strath?/Bio-Strath? is a plasmolyzed herbal yeast product with malt, honey and orange syrup. Its ability to support the immune system of the human being is well known and was tested in several studies. Equi-Strath?, the adapted product for horses was tested in this study. The aim of the study was to analyze the effects of Equi-Strath? on skin condition in the horse. The study included twenty healthy Franches-Montagnes stallions which were divided into two groups. The study group (SG, n = 10) received 0.06 ml/kg bodyweight of Equi-Strath?, the control group (CG, n = 10) the same amount of placebo, daily. The supplement and the placebo were given from week 1 until week 10 of the trial, orally. Skin biopsies were taken three times, once at the beginning of the study (week 0), once at half time (week 5) and once at the end of the study (week 10), followed by histological analysis including leucocytes count twice in five vision fields of each slide. no alterations of hair, adnexal structures or skin thickness were found. However, the number of perivascular lymphocytes turned out to be increased at the end of the study in 9 out of 10 animals of the study group, whereas number of lymphocytes in biopsies of the control group where increased only in 4 out of 10 animals. The increase of lymphocytes in the study group suggests an activation of the immune system due to the application of Equi-Strath?.

关键词： Equine Skin Biopsies Plasmolyzed Herbal Yeast Lymphocytes