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Genomic regions and pathways associated with gastrointestinal parasites resistance in Santa Inês breed adapted to tropical climate

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Journal of Animal Science and Biotechnology 2018年第1期9卷 35-50页

作者： Mariana Piatto Berton Rafael Medeiros de Oliveira Silva elisa peripolli Nedenia Bonvino Stafuzza Jesús Fernández Martin Maria Saura álvarez Beatriz Villanueva Gavina Miguel Angel Toro Georgget Banchero Priscila Silva Oliveira Joanir Pereira Eler Fernando Baldi José Bento Sterman Ferraz Departamento de Zootecnia Faculdade de Ciências Agrárias e VeterináriasUniversidade Estadual PaulistaVia de acesso Prof.Paulo Donato Castellane sno Jaboticabal SP CEP 14884 900 Brazil Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA Crta.de la Coruna km75 28040 Madrid Spain Departamento de Producción Agraria School of AgriculturalFood and Byosystems EngineeringUniversisdad Politécnica de MadridCampus Ciudad Universitaria Avda.Complutense 3-Avda.Puerta Hierro 28040 Madrid Spain Instituto Nacional de Investigación Agropecuária(INIA) Ruta 50 Kin. 12 Colonia Uruguay Faculdade de Zootecnia e Engenharia de Alimentos Nucleo de Apoio à Pesquisa em Melhoramento AnimalBiotecnologia e TransgeniaUniversidade de Sao Paulo Rua Duque de Caxias Norte 225 Pirassununga SP CEP 13635 900Brazil

Background： The aim of this study was to estimate variance components and to identify genomic regions and pathways associated with resistance to gastrointestinal parasites, particularly Haemonchus contortus, in a breed of sheep adapted to tropical climate. Phenotypes evaluations were performed to verify resistance to gastrointestinal parasites, and were divided into two categories： i） farm phenotypes, assessing body condition score（BCS）, degree of anemia assessed by the famacha chart（FAM）, fur score（FS） and feces consistency（FC）; and ii） lab phenotypes,comprising blood analyses for hematocrit（HCT）, white blood cell count（WBC）, red blood cell count（RBC）,hemoglobin（HGB）, platelets（PLT） and transformed（log10） egg per gram of feces（EPG_（log））. A total of 576 animals were genotyped with the Ovine SNP12k Bead Chip（Illumina, Inc.）, that contains 12,785 bialleleic SNP markers. The variance components were estimated using a single trait model by single step genomic BLUP ***： The overall linkage disequilibrium（LD） mean between pairs of markers measured by r2 was 0.23. The overall LD mean between markers considering windows up to 10 Mb was 0.07. The mean LD between adjacent SNPs across autosomes ranged from 0.02 to 0.10. Heritability estimates were low for EPG_（log）（0.11）, moderate for RBC（0.18）, PLT（0.17） HCT（0.20）, HGB（0.16） and WBC（0.22）, and high for FAM（0.35）. A total of 22, 21, 23, 20, 26, 25 and 23 windows for EPG_（log）for FAM, WBC, RBC, PLT, HCT and HGB traits were identified, respectively. Among the associated windows, 10 were shown to be common to HCT and HGB traits on OAR1, OAR2, OAR3, OAR5, OAR8 and ***： The traits indicating gastrointestinal parasites resistance presented an adequate genetic variability to respond to selection in Santa Inês breed, and it is expected a higher genetic gain for FAM trait when compared to the others. The level of LD estimated for markers separated by less than 1 Mb indicated that the Ovine SNP12k Bead Chip m

关键词： Gwas Linkage disequilibrium arasites resistance Santa Inês breed

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Validation of Educational Video to Promote Self-Efficacy in Preventing Childhood Diarrhea

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Health 2015年第2期7卷 192-200页

作者： Ludmila Alves do Nascimento Andressa peripolli Rodrigues Emanuella Silva Joventino Neiva Francenely Cunha Vieira Patrícia Neyva da Costa Pinheiro Lorena Barbosa Ximenes Department of Nursing Federal University of Ceará Fortaleza Brazil

The aim of this study was to evaluate and to validate an educational video to promote maternal self-efficacy in preventing childhood diarrhea with women assisted in a health unit. Validation study, developed with 17 mothers whose children were under five years old, was analyzed on scenes of an educational video according to the validity of appearance (clarity/comprehension) and of content (relevance). The scenes were considered clear and comprehensive with a perceptual above 70% and with an average of 96.9% of agreement. Regarding the relevance, the agreement was maintained between 94% and 100%, however only scenes 03 and 13 presented 76.6% and 70.6% of agreement respectively. Global Content Validation Index of the educational video scenes was 0.97 meaning an excellent level of agreement among mothers. The video showed to be a valid and a trustworthy material to promote maternal self-efficacy in preventing childhood diarrhea. Therefore, it is important that the material is disseminated in other health services so that other mothers may have contact with the same, providing greater reliability of the material.

关键词： Diarrhea Infantile Self Efficacy Educational Technology Health Education Nursing

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