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High levels of arbuscular mycorrhizal fungus colonization on Medicago truncatula reduces plant suitability as a host for pea aphids (Acyrthosiphon pisum)

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Insect Science 2020年第1期27卷 99-112页

作者： elisa Garzo Eric Rizzo Alberto Fereres S.Karen Gomez nstituto de Ciencias Agrarias-Consejo Superior de Investigaciones Cientificas(ICA-CSIC) MadridSpain School of Biological Sciences University of Northern ColoradoGreeleyColoradoUSA

This study sheds light on a poorly understood area in insect-plant-microbe interactions,focusing on aphid probing and feeding behavior on plants with varying levels of arbuscular mycorrhizal(AM)fungus root *** investigates a commonly occurring interaction of three species:pea aphid Acyrthosiphon pisum,barrel medic Medicago truncatula,and the AM fungus Rhizophagus irregularis,examining whether aphid-feeding behavior changes when insects feed on plants at different levels of AM fungus colonization(42% and 84% root length colonized).Aphid probing and feeding behavior was monitored throughout 8 h of recording using the electrical penetration graph(EPG)technique,also,foliar nutrient content and plant growth were ***,aphids took longer to reach their 1st sustained phloem ingestion on the 84% AM plants than on the 42% AM plants or on *** aphids showed phloem ingestion on the 84% AM plants relative to the 42% AM *** of the 84% AM plants had higher percent carbon(43.7%)relative to controls(40.5%),and the 84% AM plants had reduced percent nitrogen(5.3%)relative to the 42% AM plants(6%).In conclusion,EPG and foliar nutrient data support the hypothesis that modifications in plant anatomy(e.g.,thicker leaves),and poor food quality(reduced nitrogen)in the 84% AM plants contribute to reduced aphid success in locating phloem and ultimately to differences in phloem sap *** work suggests that *** plants benefit from AM symbiosis not only because of increased nutrient uptake but also because of reduced susceptibility to aphids.

关键词： Aphididae arbuscular mycorrhizal fungus electrical penetration graph(EPG) Fabaceae Glomeraceae insect-plant-microbe interactions symbiosis

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Social skills and psychopathology are associated with autonomic function in children:a cross-sectional observational study

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Neural Regeneration Research 2022年第4期17卷 920-928页

作者： elisa Cainelli Luca Vedovelli Daniele Bottigliengo Dario Boschiero Agnese Suppiej Child Neurology and Clinical Neurophysiology University Hospital of PadovaPadovaItaly Department of General Psychology University of PadovaPadovaItaly Unit of Biostatistics Epidemiology and Public HealthDepartment of CardiacThoracicVascular Sciencesand Public HealthUniversity of PadovaPadovaItaly BioTekna Biomedical Technologies Marcon(VE)Italy Department of Medical Sciences Pediatric SectionUniversity of FerraraFerraraItaly

In recent years,the increase of psychopathological disorders in the population has become a health emergency,leading to a great effort to understand psychological vulnerability *** this scenario,the role of the autonomic nervous system(ANS)has become increasingly *** study investigated the association between ANS,social skills,and psychopathological functioning in *** an ANS status proxy,we measured heart rate variability(HRV).Infants admitted to the neonatal intensive care unit of the University Hospital of Padova because of preterm birth or neonatal hypoxic-ischemic encephalopathy were sequentially recruited from January 2011 to June 2013 and followed long-term up to school age in this cross-sectional observational *** recorded 5 minutes of HRV immediately before measuring performance in social abilities tasks(affect recognition and theory of mind,NEPSY-II)in 50 children(mean age 7.4±1.4 years)with and without risk factors for developing neuropsychiatric disorders due to pre-/perinatal insults without major *** also completed extensive cognitive,neuropsychological,and psychosocial *** were assessed with psychopathological interviews and a questionnaire(CBCL 6-18).Analysis in a robust Bayesian framework was used to unearth dependencies between HRV,social skills,and psychopathological *** task scores were associated with HRV components,with high frequency the most *** bands were also associated with the psychopathological *** normalized HRV high frequency was able to distinguish impaired children in the affect recognition *** data suggest that ANS may be implicated in social cognition both in typical and atypical developmental conditions and that HRV has cross-disease *** suggest that HRV parameters may reflect a neurobiological vulnerability to *** study was approved by the Ethics Committee of the University Hospital of Padova(Comi

关键词： affect recognition autonomic nervous system children hypoxic-ischemic encephalopathy impairment neonatal prematurity psychopathology recovery theory of mind

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不破不立:利用DNA构建柔性材料

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物理 2021年第4期50卷 268-270页

作者：任春来(编译) Davide michieletto 南京大学不详

DNA分子间不是固定的——它们会不断拆分和结合来形成新的形态。Davide michieletto解释了如何利用DNA这一特点来制造出新一代“拓扑活性”材料。不是我不明白,这世界变化快!直到几年前我都没想过DNA还可以买卖。作为一个物理学家,我自... 详细信息

DNA分子间不是固定的——它们会不断拆分和结合来形成新的形态。Davide michieletto解释了如何利用DNA这一特点来制造出新一代“拓扑活性”材料。不是我不明白,这世界变化快!直到几年前我都没想过DNA还可以买卖。作为一个物理学家,我自诩熟知DNA--“生命分子”:它们携带的遗传信息使得复杂的生命体,比如你和我,得以构建出来。

关键词：柔性材料遗传信息不破不立 DNA 生命体物理学家

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Experimental study on the optimum design of diffuser-augmented horizontal-axis tidal turbine

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Clean Energy 2022年第5期6卷 776-786页

作者： Evi elisa Ambarita Harinaldi Rifqi Azhari Ridho Irwansyah Department of Mechanical Engineering Universitas IndonesiaKampus UI Depok 16424Indonesia

Ocean current energy is a promising and reliable resource that offers sustainability and predictability in realizing green future needs.A diffuser-augmented horizontal-axis turbine is utilized to generate ocean current energy.A numerical study on the impact of diffuser angle variations on the power coefficient has been carried out in the previous *** elucidate the fluid dynamics aspects and further validation of previous computational results,the experimental investigation on the optimal design of tidal turbine with 20.04°diffuser augmentation is presented in this *** study was conducted in a flowing tank with a current velocity of 0.7 m/*** maximum power coefficient of 20.04°is 0.436 experimentally,which is a little smaller than the numerical ***,to reinforce the 20.04°result,a diffuser with an angle of 10.43°was also manufactured and tested *** maximum power coefficient of 10.43°is 0.303 experimentally,which is 3%smaller than the numerical *** was concluded that the numerical approach might be considered satisfactory and represent similar phenomena to the experimental investigation in an application for modelling of multi-objective optimization.

关键词： ocean current energy tidal turbine diffuser augmentation power coefficient experimental study

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Evaluation of mercury biogeochemical cycling at the sediment–water interface in anthropogenically modified lagoon environments

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Journal of Environmental Sciences 2018年第6期30卷 5-23页

作者： elisa Petranich Stefano Covelli Alessandro Acquavita Jadran Faganeli Milena Horvat Marco Contin Dipartimento di Matematica e Geoscienze Universita degli Studi di Trieste Via Weiss 2 34128 Trieste Italy Dipartimento di Scienze della Vita Universita degli Studi di Trieste Via Licio Giorgieri 5 34128 Trieste Italy CoN.I.S.Ma. Consorzio Nazionale Interuniversitario per le Scienze del Mare Piazzale Flaminio 9 00196 Rome Italy Agenzia Regionale per la Protezione Ambientale del Friuli Venezia Giulia ARPA FVC Via Cairoli 14 33057 Palmanoua (Udine) Italy Marine Biological Station Fornace 41 6330 Piran Slovenia Department of Environmental Sciences Joker Stefan Institute Jamova 39 1000 Ljubljana Slovenia Dipartimento di Scienze Agro-Alimentari Ambientali e Animali Universita di Udine Via delle Scienze 206 33100 Udine Italy

The Marano and Grado Lagoon is well known for being contaminated by mercury（Hg） from the Idrija mine（Slovenia） and the decommissioned chlor-alkali plant of Torviscosa（Italy）.Experimental activities were conducted in a local fish farm to understand Hg cycling at the sediment–water interface. Both diffusive and benthic fluxes were estimated in terms of chemical and physical features. Mercury concentration in sediments（up to 6.81 μg/g）showed a slight variability with depth, whereas the highest methylmercury（MeHg） values（up to 10 ng/g） were detected in the first centimetres. MeHg seems to be produced and stored in the 2–3 cm below the sediment–water interface, where sulphate reducing bacteria activity occurs and hypoxic–anoxic conditions become persistent for days. DMeHg in porewaters varied seasonally（from 0.1 and 17% of dissolved Hg（DHg）） with the highest concentrations in summer. DHg diffusive effluxes higher（up to 444 ng/m^2/day） than those reported in the open lagoon（~ 95 ng/m^2/day）, whereas DMeHg showed influxes in the fish farm（up to-156 ng/m^2/day）. The diurnal DHg and DMeHg benthic fluxes were found to be higher than the highest summer values previously reported for the natural lagoon environment. Bottom sediments, especially in anoxic conditions, seem to be a significant source of MeHg in the water column where it eventually accumulates. However, net fluxes considering the daily trend of DHg and DMeHg, indicated possible DMeHg degradation processes. Enhancing water dynamics in the fish farm could mitigate environmental conditions suitable for Hg methylation.

关键词： Mercury Benthic fluxes Biogeochemistry lagoons Sediment–water interface

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Protecting the photosynthetic performance of snap bean under free air ozone exposure

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Journal of Environmental Sciences 2018年第4期30卷 31-40页

作者： Lu Zhang Yasutomo Hoshika elisa Carrari Kent O.Burkey Elena Paoletti College of Horticulture Northeast Agricultural University Institute of Sustainable Plant Protection National Research Council Plant Science Research Unit USDA-ARS

Tropospheric ozone（O3） is a major air pollutant and causes serious injury to vegetation. To protect sensitive plants from O3 damage, several agrochemicals have been assessed,including cytokinin（e.g., kinetin, KIN） and ethylenediurea（EDU） with cytokinin-like *** higher plant, leaves are primarily injured by O3 and protective agrochemicals are often applied by leaf spraying. To our knowledge, the mitigating abilities of EDU and KIN have not been compared directly in a realistic setup. In the present research, impacts of elevated O3（2 × ambient O3, 24 hr per day, for 8 days） on an O3 sensitive line（S156） of snap bean（Phaseolus vulgaris）, which is often used for biomonitoring O3 pollution, were studied in a free air controlled exposure system. The day before starting the O3 exposure, plants were sprayed with a solution of EDU（300 ppm）, KIN（1 mmol/L） or distilled water, to compare their protective abilities. The results demonstrated that 2 × ambient O3 inhibited net photosynthetic rate and stomatal conductance, increased the minimal fluorescence yield of the dark-adapted state, decreased the maximal quantum yield of PSII photochemistry, and led to visible injury. KIN and EDU alleviated the reduction of the photosynthetic performance, and visible injury under O3 fumigation. The plants sprayed with EDU showed greater ability to mitigate the O3 damage than those sprayed with KIN. Chlorophyll fluorescence imaging may have detected more precisely the differences in O3 response across the leaf than the conventional fluorometer.

关键词： Ethylenediurea Free air controlled exposure Kinetin Ozone Phaseolus vulgaris

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X-ray diffraction investigation of amorphous calcium phosphate and hydroxyapatite under ultra-high hydrostatic pressure

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International Journal of Minerals,Metallurgy and Materials 2015年第11期22卷 1225-1231页

作者： elisa Lam Qinfen Gu Peter J.Swedlund Sylvie Marchesseau Yacine Hemar School of Chemical Sciences The University of Auckland Australian Synchrotron Université Montpellier 2 UMR IATE Pl.E Bataillon34095 Montpellier cedex 5 France

The changes in the crystal structures of synthetically prepared amorphous calcium phosphate（ACP） and hydroxyapatite（HAP） in water（1：1 mass ratio） were studied by synchrotron X-ray diffraction（XRD） under ultra-high hydrostatic pressures as high as 2.34 GPa for ACP and 4 GPa for HAP. At ambient pressure, the XRD patterns of the ACP and HAP samples in capillary tubes and their environmental scanning electron micrographs indicated amorphous and crystalline characteristics for ACP and HAP, respectively. At pressures greater than 0.25 GPa, an additional broad peak was observed in the XRD pattern of the ACP phase, indicating a partial phase transition from an amorphous phase to a new high-pressure amorphous phase. The peak areas and positions of the ACP phase, as obtained through fitting of the experimental data, indicated that the ACP exhibited increased pseudo-crystalline behavior at pressures greater than 0.96 GPa. Conversely, no structural changes were observed for the HAP phase up to the highest applied pressure of 4 GPa. For HAP, a unit-cell reduction during compression was evidenced by a reduction in both refined lattice parameters a and c. Both ACP and HAP reverted to their original structures when the pressure was fully released to ambient pressure.

关键词： calcium phosphate hydroxyapatite high hydrostatic pressure X-ray diffraction

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Testing visible ozone injury within a Light Exposed Sampling Site as a proxy for ozone risk assessment for European forests

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Journal of Forestry Research 2021年第4期32卷 1351-1359页

作者： Pierre Sicard Yasutomo Hoshika elisa Carrari Alessandra De Marco Elena Paoletti ARGANS 260 route du Pin Montard06410 BiotFrance IRET-CNR Via Madonna del Piano 1050019 Sesto FiorentinoItaly Department of Agriculture FoodEnvironment and Forestry(DAGRI)University of FlorenceFlorenceItaly ENEA Via Anguillarese 30100123 Santa Maria di GaleriaItaly

Biologically meaningful and cost-effective indicators are needed for assessing and monitoring the impacts of tropospheric ozone(0_(3)) on vegetation and are required in Europe by the National Emission Ceilings Directive(2016).However,a clear understanding on the best suited indicators is *** MOTTLES(MOnitoring ozone injury for seTTing new critical LEvelS) project set up a new generation network for 0_(3) monitoring in forest plots in order to:1) estimate the stomatal 0_(3) fluxes(Phytotoxic Ozone Dose above a threshold Y of uptake,PODY);and 2) collect visible foliar 0_(3) injury,both within the forest plot(ITP) and along the Light Exposed Sampling Site(LESS) along the forest *** forest sites at high 0_(3) risk were selected across Italy over 2017-2019 and significant correlations(p <0.05) were found between the percentage of symptomatic plant species within the LESS,and POD1(PODY,with Y=1 nmol 0_(3) m^(-2) s^(-1)) calculated for mixed forest species(r=0.53)and with the occurrence and severity of visible foliar 0_(3) injury on the dominant species in the plots(r=0.65).A generic flux-based critical level for mixed forest species was derived within the LESS and it was recommended using11 mmol m^(-2) POD1 as the critical level for forest protection against 0_(3) injury,similar to the critical level obtained in the ITP(12 mmol m^(-2) POD1).It was concluded that the frequency of symptomatic plant species within a LESS is a suitable and effective plant-response indicator of phytotoxic 0_(3) levels in forest *** is a non-destructive,less complex and less time-consuming approach compared to the ITP for monitoring foliar 0_(3) injury in the long *** visible foliar 0_(3) injury in the ITP might only underestimate the 0_(3) risk assessment at individual *** results are biologically meaningful and useful to monitoring experts and environmental policy makers.

关键词： Cost-effective indicator Forest monitoring Light-Exposed Sampling Site Ozone Phytotoxic ozone dose Visible injury

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Compatibility of early natural enemy introductions in commercial pepper and tomato greenhouses with repeated pesticide applications

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Insect Science 2020年第5期27卷 1111-1124页

作者： Beatriz Dader Ignacio Colomer Angeles Adan Pilar Medina elisa Vinuela Unidad de Proteccion de Cultivos Escuela Tecnica Superior de Ingenieria AgronomicaAlimentaria y de Biosistemas(ETSIAAB)Universidad Polit^cnica de Madrid(UPM)MadridSpain Departamento de Ingenieria Rural Escuela Superior de IngenieriaUniversidad de AlmeriaAlmeriaSpain

Successful integrated pest management in protected crops implies an evalu-ation of the compatibility of pesticides and natural enemies(NE),as control strategies that only rely on one tactic can fail when pest populations exceed NE activity or pests become resistant to *** in Almeria(Spain),growers release NE prior to transplanting or early in the crop cycle to favor their settlement before pest arrival because this improves biocontrol efficacy,although it extends pesticide exposure *** pur-pose of this research was to evaluate the compatibility of two applications of pesticides with key NE in 2-year trials inside tomato and sweet pepper commercial greenhouses:Nesidiocoris tenuis(Reuter)(Hemiptera:Miridae),Orius laevigatus(Say)(Hemiptera:Anthocoridae)and Amblyseius swirskii(Athias-Henriot)(Acari:Phytoseidac).In tomato,flubendiamide and chlorantraniliprole(IOBC category 1)were compatible with ***,but chlorpyrifos.:methyl and spinosad(IOBC categories 2-3),which efectively reduced Tiuta absoluta(Meyrick)(Lepidoptera:Gelechidae)density,compromised its predatory *** sweet pepper,chlorantraniliprole(IOBC category 1)was the only pesticide compatible with *** while chlorantraniliprole,emamectin benzoate,spirote-tramat and pymetrozine were harmless(IOBC category 1)to Amblyseius swirskii,and sulfoxaflor slightly harmful(IOBC category 2)to this phytoseiid predator.

关键词： Amblyseius swirskii chemical control Frankliniella occidentalis Nesidiocoris tenuis Orius laevigatus Tuta absoluta

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One Decade of Development and Evolution of MicroRNA Target Prediction Algorithms

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Genomics, Proteomics & Bioinformatics 2012年第5期10卷 254-263页

作者： Paula H.Reyes~Herrera elisa Ficarra School of Electronic and Biomedical Engineering Universidad Antonio Narino Department of Control and Computer Engineering Politecnico di Torino

Nearly two decades have passed since the publication of the first study reporting the discovery of microRNAs （miRNAs）. The key role of miRNAs in post-transcriptional gene regulation led to the performance of an increasing number of studies focusing on origins, mech- anisms of action and functionality of miRNAs. In order to associate each miRNA to a specific functionality it is essential to unveil the rules that govern miRNA action. Despite the fact that there has been significant improvement exposing structural characteristics of the miRNA-mRNA interaction, the entire physical mechanism is not yet fully understood. In this respect, the development of computa- tional algorithms for miRNA target prediction becomes increasingly important. This manuscript summarizes the research done on miRNA target prediction. It describes the experimental data currently available and used in the field and presents three lines of compu tational approaches for target prediction. Finally, the authors put forward a number of considerations regarding current challenges and future directions.

关键词： MicroRNAs MicroRNA recognition elements Target prediction

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