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Advances in Microbiology 2015年第1期5卷 40-53页

作者： Lidia Sas Paszt eligio malusá Beata Sumorok Loredana Canfora Edyta Derkowska Slawomir Gluszek Research Institute of Horticulture SkierniewicePoland Consiglio per la Ricerca e Sperimentazione in Agricoltura RomeItaly

The new products obtained from natural resources are an alternative to methods based on traditional mineral fertilizers, which are destructive for soil mycorrhizal communities. Our experiment was carried out to evaluate the effect of organic fertilizers and amendments of very diverse composition on mycorrhizal abundance and diversity, as well as on root growth, in strawberry plants cv. “Honeoye”. The plants were grown in rhizoboxes filled with a podsolic soil. The plants were treated with granulated bovine manure, vermicompost extract, humates extract, plant extract, extract from seaweed species reinforced with humic and fulvic acids, a consortium of beneficial soil organisms, a stillage from yeast production and a solution of titanium. Plants treated with products and the microorganisms consortium also received half dose of manure. A standard mineral fertilization (NPK) and an unfertilized control were also included. The bioproducts based on humus-like substances and the yeast stillage had the greatest positive influence on the colonization of roots by arbuscular mycorrhizal fungi (AMF). The different treatments affected the diversity of AMF species present in the rhizospheric soil. All organic products, even though providing a significantly low amount of nutrients, enhanced root growth characteristics in comparison to the mineral fertilization.

关键词： AMF Mycorrhizal Frequency Organic Fertilizers Root Morphology

Efficacy of Magnesium and Alpha Lipoic Acid Supplementation in Reducing Premature Uterine Contractions

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Open Journal of Obstetrics and Gynecology 2014年第9期4卷 578-583页

作者： eligio Parente Giulia Colannino Pasquale Ferrara Clinic Center “Trusso” Naples Italy Obstetric and Gynecological Center “Villa delle Querce” Naples Italy

Objective: Premature uterine contractions represent one of the major symptoms related to preterm labor. So far, primary prevention of preterm labor is based on the early identification of symptoms and on pharmacological treatments which are prone of several secondary effects. In this double-blinded, randomized, placebo-controlled trial, the efficacy of a supplementation of magnesium and alpha-lipoic acid has been evaluated. Methods: Three hundred pregnant women at 14 - 34 weeks of gestation were enrolled and randomly divided to receive a daily single tablet containing a supplement of magnesium and alpha-lipoic acid (DAV®LoLiPharmasrl, Rome-Italy) or placebo until delivery. The incidence of episodes of preterm uterine contraction, associated or not with pain, as well as maternal need of hospitalization was evaluated. Results: Magnesium and lipoic acid supplementation was effective to significantly reduce the incidence of preterm uterine contractions compared to placebo. In particular, 52% of women who received the supplementation reported no symptoms of preterm uterine contractions throughout pregnancy, and persistent episodes of uterine contractions were significantly reduced compared to placebo (20% vs 60%, respectively). Furthermore, only 20% of subjects who received the supplementation required hospitalization, while it has been necessary for 40% of women who received placebo. Conclusions: Our findings suggest that supplementation with magnesium and lipoic acid is effective in reducing the incidence of premature uterine contraction and related episodes of hospitalization, compared to placebo. Nevertheless, further studies based on larger cohorts of patients are necessary to confirm the efficacy of these preliminary results.

关键词： Magnesium Alpha-Lipoic Acid Preterm Labor Tocolysis Pregnancy

Intestinal parasitic infections in Aetas and domesticated swine in Pampanga,Philippines

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Asian Pacific Journal of Tropical Medicine 2022年第11期15卷 522-524页

作者： Jessica Joyce R De Guia Mary Jane C Flores Derick Erl P Sumalapao eligio Santiago V Maghirang Vicente Y Belizario Jr. Department of Biology College of ScienceDe La Salle UniversityManilaPhilippines Biological Control Research Unit(BCRU) Center for Natural Sciences and Environmental Research(CENSER)College of ScienceManila Ehime University-De La Salle University International Collaborative Research Laboratory De La Salle UniversityLaguna CampusLaguna ProvincePhilippines Department of Epidemiology and Biostatistics College of Public HealthUniversity of the PhilippinesManilaPhilippines Department of Internal Medicine-Section of General Medicine Department of Medical Education and Research the Makati Medical Center Department of Emergency Medicine Asian Hospital and Medical CenterMetro ManilaPhilippines Department of Parasitology College of Public HealthUniversity of the PhilippinesManilaPhilippines

Indigenous peoples account for about 6%of the world’s population and are highly vulnerable to parasitic infections[1,2].Aetas,one of the indigenous people groups in the Philippines,rely on backyard swine production as their primary means of livelihood[3].Literature indicated that zoonoses arise from close physical contact and the cohabitation of humans with domesticated animals[4].Ascaris spp.

关键词： swine domestica habitat

Assemblages of <i>Giardia duodenalis</i>Isolates from Dogs by Amplification and Restriction of tpi and <i>β</i>-Giardin Genes and Sequencing of SSU rDNA Gene

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Open Journal of Veterinary Medicine 2013年第3期3卷 222-227页

作者： Leticia eligio-Garcia Cortés-Campos Adrian Cano-Estrada Apolinar Jiménez-Cardoso Enedina Laboratorio de Investigación en Parasitología Children Hospital of MéxicoMéxico D.F. México

Giardia duodenalis exhibits seven assemblages (A-G) that are distributed in different hosts. The A and B assemblages are commonly found in humans and several mammals, while C and D assemblages are typically found in dogs. The purpose of this study was to determine the assemblage of Giardia duodenalis present in the stool samples of ten canines using an assay based on PCR amplification, restriction analysis, and sequencing of the small subunit ribosomal DNA (SSU-rDNA), β-giardin, and triosephosphate isomerase (tpi) genes in order to establish the similarities or differences between the assemblage obtained with each gene. The results indicated that all positive isolates belonged to assemblage A, and specifically to the sub-assemblage A-I. A comparison of the SSU-rDNA gene sequence revealed the presence of three subgroups of assemblage A. These findings highlight the importance of canine transmission of Giardia in Mexico and its genetic plasticity. They also establish a method for additional and more molecularly extensive epidemiological studies to improve sanitation and hygiene in the most affected areas.

关键词： Giardia duodenalis Genotyping Zoonosis tpi β-Giardin SSU rDNA

The Influence of Mycorrhizal Fungi on the Growth of Apple and Sour Cherry Maidens Fertilized with Different Bioproducts in the Organic Nursery

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Journal of Life Sciences 2015年第5期9卷 221-228页

作者： Zygmunt S. Grzyb Lidia Sas Paszt Wojciech Piotrowski eligio malusa Department of Pomology Research Institute of Horticulture Konstytucji 3 Maja 1/3 Skierniewice 96-100 Poland CRA-Center for Plant Soil Systems. Livorno 58 Torino 10100 Italy

Apple/malus x domestica cv. ＇Topaz＇/M26 and sour cherry cv. ＇Debreceni Botermo＇/Prunus mahaleb L. maidens were fertilized with different organic fertilizers and soil amendments in an organic nursery. A mycorrhizal inoculant, Micosat, was added to the non-fertilized control and to combinations fertilized with various bioproducts. Under assessment was the effect of nutrient delivery on the growth and development of maiden fruit trees focussing on tree height, thickness of the tree trunk, the number and the length of lateral shoots in each treatment combination. It was found that the introduction of mycorrhizal fungi into the soil without fertilizer application significantly improved the growth and development of apple and sour cherry maidens. Mineral fertilizer application （NPK） combined with mycorrhizal inoculation had a negligible effect on the above features. In the presence of bioproducts such as granular manure, Humus Active ＋ Aktywit PM, BF Amin, Vinassa and Florovit Eko, the mycorrhizal fungi were more biologically active than in the absence of these products.

关键词： Biopreparation fertilization maiden fruit tree mycorrhiza ecological nursery.

Changes in leaflet shape and seeds per pod modify crop growth parameters, canopy light environment, and yield components in soybean

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The Crop Journal 2020年第2期8卷 351-364页

作者： Julieta S.Bianchi Alvaro Quijano Carlos O.Gosparini Laboratorio de Fisiología Vegetal Facultad de Ciencias AgrariasUniversidad Nacional de Rosario(UNR)Campo Experimental J.VillarinoP.O.Box 142125 ZavallaSanta FeArgentina Instituto de Investigaciones en Ciencias Agrarias de Rosario(IICAR) Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)–UNRCampo Experimental J.VillarinoP.O.Box 142125 ZavallaSanta FeArgentina Consejo de Investigaciones de la UNR(CIUNR) Campo Experimental J.VillarinoP.O.Box 142125 ZavallaSanta FeArgentina

Seed number(SN)is the component most strongly associated with yield in *** depends on pod number(PN)and seeds per pod(SPP).Whereas SPP is a relatively stable component,PN is strongly influenced by environmental and management ***,the environmental cues involved in PN regulation are not completely *** influence of increasing SPP on other yield components is *** trials were conducted in two growing seasons using two pairs of lanceolate(L)and ovate(O)nearisogenic lines,sown at low(LD)and high(HD)plant densities to evaluate the effect of leaflet shape on crop growth parameters,canopy red/far-red(R/FR)ratio,their relationships with pod initiation,PN,and yield;and the effect of increasing SPP on PN,SN,and yield.L canopies showed a higher number of pods initiated(PI)than O canopies owing to the increase of PI on *** association between PI and crop growth rate during the pod set period was *** was negatively associated with leaf area index(LAI)and light *** contrast,a positive association between PI and canopy R/FR ratio was *** latter association was sustained irrespective of whether the LAI was below or above its critical value,providing experimental evidence that R/FR ratio is positively associated with pod initiation in soybean *** increase in SPP produced a direct and steady increase in SN regardless of PN and plant density.A yield increase was observed for the L-LD treatment,which combined the increased SPP of L lines with the highest PN of L-LD *** results have implications for crop management and breeding strategies aimed at increasing the yield potential of soybean.

关键词： soybean breeding shape

In vitro Antibacterial Activity of Moringa oleifera Ethanolic Extract against Tomato Phytopathogenic Bacteria

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Phyton-International Journal of Experimental Botany 2021年第3期90卷 895-906页

作者： Roberto Arredondo-Valdés Francisco D.Hernández-Castillo Mario Rocandio-Rodríguez Julia C.Anguiano-Cabello Madai Rosas-Mejía Venancio Vanoye-eligio Salvador Ordaz-Silva Imelda V.López-Sánchez Laura D.Carrazco-Peña Julio C.Chacón-Hernández Institute of Applied Ecology Universidad Autónoma de TamaulipasCiudad VictoriaTamaulipas87019México Parasitology Department Universidad Autónoma Agraria Antonio NarroSaltillo25315México Health Area Universidad La Salle SaltilloSaltillo25298México Faculty of Engineering and Business San Quintin Universidad Autónoma de Baja CaliforniaSan Quintín22930México

The tomato(Solanum lycopersicum L.)is one of the world’s most important vegetable ***,phytopathogenic bacteria affect the yield and quality of tomato cultivation,like Agrobacterium tumefeciens(At),Clavibacter michiganensis ***(Cmm),Pseudomonas syringae ***(Pst),Ralstonia solanacearum(Rs),and Xanthomonas axonopodis(Xa).Synthetic chemical products are used mostly on disease plant control,but overuse generates resistance to bacterial *** study aimed to evaluate the in vitro antibacterial activity of the ethanolic extract of Moringa oleifera *** against At,Cmm,Pst,Rs,and Xa,as well as information about this plant species’chemical *** activity against pathogens observed by microplate technique,phytochemical screening,and FTIR analysis revealed different bio-active compounds on ethanolic extracts with antibacterial *** growth inhibition rate ranged between 0.08%and 99.94%.The inhibitory concentration,IC50,required to inhibit 50%of At,Cmm,Pst,Rs,and Xa bacterial growth,was 276.67,350.48,277.85,351.49,and 283.22 mg/L,*** of phytopathogen bacteria’s growth increased as the concentrations of the extract also *** oleifera extract can be recommended as a potent bio-bactericide.

关键词： Biological control secondary metabolites tomato crop inhibitory concentration botanical extract

Frequency of Emerging Parasites in HIV/AIDS and Oncological Patients Stool by Coprological and Molecular Analysis

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Advances in Infectious Diseases 2013年第3期3卷 162-171页

作者： Enedina Jiménez-Cardoso Leticia eligio-García Apolinar Cano-Estrada Adrian Cortés-Campos Aurora Medina-Sansón Diana Molina-Martínez Children Hospital of Mexico Mexico City Mexico

The purpose of this study was to determine the frequency of emerging parasites in two groups of immunosuppressed patients, including individuals infected with the human immunodeficiency virus (AIDS) (HIV) or having acute lymphoblastic leukemia (ALL) with or without diarrhea. Stool samples were collected from 96 HIV and 77 ALL patients from March 2010 through December 2011. Screening for opportunistic parasites was carried out by the coproparasitoscopic Faust method, Ziehl Neelsen staining, and Polymerase Chain Reaction (PCR). Results showed that 22.9% of HIV fecal samples were positive for emerging parasites, including Cryptosporidium spp. (7.3%), Microsporidium spp. (5.2%), Isospora belli (1.0%), Giardia intestinalis (2.6%), and Cyclospora spp. (7.3%). On the other hand, 32.5% of ALL fecal samples were positive for emerging parasites, including Cryptosporidium spp. (9.1%), Microsporidium spp. (19.5%), Isospora belli (1.3%), and Giardia intestinalis (2.6%). Our results highlighted the need for specific, efficient, and reliable diagnostic methods to identify the presence of emerging parasites in immunocompromised patients susceptible to different infectious diseases or neoplastic processes and avoid the consequences for the host as an increased disease rate, alterations in the clinical manifestation of the infection or even exacerbation of its course.

关键词： Leukemia HIV Emerging Parasites Microsporidium Cryptosporidium spp. Giardia Intestinalis Cyclospora Isospora Belli

The frequency of intestinal parasites in puppies from Mexican kennels

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Health 2010年第11期2卷 1316-1319页

作者： Enedina Jiménez-Cardoso Leticia eligio-García Adrián Cortés-Campos Apolinar Cano Estrada Margarita Pinto-Sagahón Cynthia Noguera-Estrada 不详

The purpose of this investigation was to determine the intestinal parasite prevalence in puppies from six different kennels;four kennels were in Guadalajara and Zapopan cities (Jalisco State) and two kennels were in Mexico City. From October 2006 to November 2007, we collected 441 fecal samples from 147 puppies, both male and female, ranging from 1 to 36 months of age. Three samples from every puppy were analyzed by using the Faust technique. The prevalence found were as follows: Giardia intestinalis (genotype A and B) 6.8%;Cystoisospora 21.08%;Uncinaria 7.48%;Toxocara canis 12.29% and multiparasitism (Giardia, Toxocara and Uncinaria) 4.76%. The highest prevalence for both Giardia and Cystoisospora were found in 2-3- month-old puppies;the highest prevalence for Toxocara canis was found in 3-4-month-old puppies. In the kennels of Mexico City we found mainly Giardia intestinalis, Cystoisospora to be most prevalent in Zapopan and Toxocara canis in Guadalajara. The high prevalence of intestinal parasites found in this study demonstrates an increased risk for infection in humans, as these animals are usually a common pet in many homes. This zoonotic phenomenon represents an important health problem for any community.

关键词： Parasites Giardia Cystoisospora Dogs

Preemptive endoluminal vacuum therapy after pancreaticoduodenectomy: A case report

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World Journal of Gastrointestinal Endoscopy 2020年第11期12卷 493-499页

作者： Flaubert Sena de Medeiros Epifanio Silvino do Monte Junior Romero de Lima França Heli Clóvis de Medeiros Neto Juliany Medeiros Santos eligio Alves Almeida Júnior Samuel Oliveira da Silva Júnior Mario Herman Santos Moura Pedreira Tavares Eduardo Guimarães Hourneaux de Moura Department of Surgery Federal University of Rio Grande do NorteNatal 59012-300Rio Grande do NorteBrazil Gastrointestinal Endoscopy Unit Hospital das Clínicas da Faculdade de Medicina da Universidade de São PauloSão Paulo 05403-000Brazil Department of Surgery Hospital do CoraçãoNatal 59075-050Rio Grande do NorteBrazil Gastrointestinal Endoscopy Unit Faculty of Medicine of ABCSão Paulo 09190-615Brazil Department of Surgery Instituto Juarez AlmeidaBacabal 65700-000MaranhãoBrazil Department of Surgery Hospital Naval Marcílio DiasRio de Janeiro 20725-090Rio de JaneiroBrazil

BACKGROUND Pancreaticoduodenectomy is a technically demanding operation,with reported morbidity rates of approximately 40%-50%.A novel idea is to use endoscopic vacuum therapy(EVT)in a preemptive setting to prevent anastomotic leakage and pancreatic *** a recent case series,EVT was proven to be effective in preventing leaks in patients with anastomotic *** have been no previous reports on preemptive EVT after *** SUMMARY We describe the case of a 71-year-old woman with hypertension and diabetes who was admitted to the emergency room with jaundice,choluria,fecal acholia,abdominal pain,and *** examinations revealed leukocytosis and hyperbilirubinemia(total:13 mg/dL;conjugated:12.1 mg/dL).Abdominal ultrasound showed cholelithiasis and dilation of the common bile *** resonance imaging demonstrated a stenotic area,and a biopsy confirmed cholangiocarcinoma. Considering the high risk of leaks after pancreaticoduodenectomy,preemptive endoluminal vacuum therapy was *** system comprised a nasogastric tube,gauze,and an antimicrobial incise *** negative pressure was 125 mmHg,and no adverse events *** patient was discharged on postoperative day 5 without any *** Preemptive endoluminal vacuum therapy may be a safe and feasible technique to reduce leaks after pancreaticoduodenectomy.

关键词： Preemptive Endoluminal Vacuum Pancreaticoduodenectomy Case report