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Realization Feature of Mesenchymal Dermal Cells Tissue Engineering Construction Response in Granulating Wound Transplantation in Relation with Time-Frame

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Journal of Cosmetics, Dermatological Sciences and Applications 2012年第3期2卷 126-129页

作者： elena petersen Moscow Scientific Research Center of Dermatovenerology and Cosmetology of Moscow City Moscow Russia

Derma is progenitor cells sours, that are able to differentiate further in several mesodermal lineage and neural and endodermal lineage. Culture conditions, skin taking site and culture medium composition considerably contribute to it. Spheroid cultured mesenchymal dermal cells contribution to skin regeneration in granulating wound in rat model was estimated.

关键词： Brief Report 3D Cultivated Tissue Engineering Construction Skin Mesenchymal Dermal Cells Sprouting Capillary-Like Structures

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Cytotoxicity and non-specific cellular uptake of bare and surface-modified upconversion nanoparticles in human skin cells

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Nano Research 2015年第5期8卷 1546-1562页

作者： Anna E. Guller Alia N. Generalova elena V. petersen Andrey V. Nechaev Inna A. Trusova Nikolay N. Landyshev Annemarie Nadort Ekaterina A. Grebenik Sergey M. Deyev Anatoly B. Shekhter Andrei V. Zvyagin Macquarie University Australia I.M. Sechenov First Moscow State Medical University Russia Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences Russia Nizhny Novgorod State University Russia Moscow Institute of Physics and Technology Russia Moscow State University of Fine Chemical Technologies Russia University of Amsterdam the Netherlands

The cytotoxicity and non-specific cellular uptake of the most popular composition of upconversion nanoparticle （UCNP）, NaYF4：Yb^3＋：Er^3＋, is reported using normal human skin cells, including dermal fibroblasts and immortalized human epidermal linear keratinocytes （HaCaT）. A new hydrophilization reaction of as-synthesized UCNPs based on tetramethylammonium hydroxide （TMAH） enabled evaluation of the intrinsic cytotoxicity of bare UCNPs. The cytotoxicity effects of the UCNP surface-coating and polystyrene host were investigated over the concentration range 62.5-125 μg/mL with 24-h incubation, using a MTT test and optical microscopy. The fibroblast viability was not compromised by UCNPs, whereas the viability of keratinocytes varied from 52% ± 4% to 100% ± 10% than the control group, depending on the surface modification. Bare UCNPs reduced the keratinocyte viability to 76% ± 3%, while exhibiting profound non-specific cellular uptake. Hydrophilic poly（D,L-lactide）- and poly（maleic anhydride-alt-l-octadecene）-coated UCNPs were found to be least cytotoxic among the polymer-coated UCNPs, and were readily internalized by human skin cells. Polystyrene microbeads impregnated with UCNPs remained nontoxic. Surprisingly, no correlation was found between UCNP cytotoxicity and the internalization level in cells, although the latter ranged broadly from 0.03% to 59%, benchmarked against 100% uptake level of TMAH-UCNPs.

关键词： nanoparticle upconversion surface modification biocompatibility cytotoxicity human skin

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