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Labeling of influenza viruses with synthetic fluorescent and biotin-labeled lipids

Virologica Sinica 2014年第4期29卷 199-210页

作者： Natalia A Ilyushina Evgeny S Chernyy elena y korchagina Aleksra S Gambaryan Stephen M Henry Nicolai V Bovin Food and Drug Administration 29 Lincoln DriveBethesda 20892 MD USA Shemyakin Institute of Bioorganic Chemistry Russian Academy of Sciences Miklukho Maklaya 16/10Moscow 117997 Russia Chumakov Institute of Poliomyelitis and Viral Encephalitides Russian Academy of Medical SciencesMoscow Region 142782 Russia Biotechnology Research Institute AUT University Private Bag 92006Auckland 1142 New Zealand

Direct labeling of virus particles is a powerful tool for the visualization of virus–cell interaction events. However, this technique involves the chemical modification of viral proteins that affects viral biological properties. Here we describe an alternative approach of influenza virus labeling that utilizes Function-Spacer-Lipid(FSL) constructs that can be gently inserted into the virus membrane. We assessed whether labeling with fluorescent(fluo-Ad-DOPE) or biotin-labeled(biot-CMG2-DOPE) probes has any deleterious effect on influenza virus hemagglutinin(HA) receptor specificity, neuraminidase(NA) activity, or replicative ability in vitro. Our data clearly show that neither construct significantly affected influenza virus infectivity or viral affinity to sialyl receptors. Neither construct influenced the NA activities of the influenza viruses tested, except the A/Puerto Rico/8/34(H1N1) strain. Our data indicate that lipid labeling provides a powerful tool to analyze influenza virus infection in vitro.

关键词： bioimaging Function-Spacer-Lipid (FSL) constructs labeling of influenza viruses synthetic lipids

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The Synthetic Diamond/Pyrolytic Carbon Nanocomposites: A Study Using Lithium Intercalation

The Synthetic Diamond/Pyrolytic Carbon Nanocomposites: A Stu...

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2～(nd) Spring Meeting of the International Society of Electrochemistry

作者： yu.Pleskov T.Kulova A.Skundin M.Krotova V.Ralchenko S.korchagina S.Gordeev Frumkin Institute of Electrochemistry Moscow Russia Frumkin Institute of Electrochemistry Moscow Russia Frumkin Institute of Electrochemistry Moscow Russia Frumkin Institute of Electrochemistry Moscow Russia General Physics Institute Moscow Russia Central Research Institute for Materials St.Petersburg Russia Central Research Institute for Materials St.Petersburg Russia

Electrochemical behavior of two diamond-based composites is studied by the cathodic Liintercalation from LiPF6 solution in the polypropylene carbonate-diethyl carbonate （1:4）mixture. In the NDC composite, diamond nanocrystals （4-5 nm in size） are bound up with apyrolytic carbon matrix which coats the diamond crystals with a thin （～1 nm） layer. Thenondiamond carbon content is 9-28%;the specific surface area, 220 to 350 m/g. In the Carbalcomposite, the submicron diamond crystals were 0.3 um in size;the nondiamond carbon

关键词： A Study Using Lithium Intercalation The Synthetic Diamond/Pyrolytic Carbon Nanocomposites

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