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M1-polarized macrophage-derived cellular nanovesicle-coated lipid nanoparticles for enhanced cancer treatment through hybridization of gene therapy and cancer immunotherapy

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Acta Pharmaceutica Sinica B 2024年第7期14卷 3169-3183页

作者： ha Eun Shin Jun-Hyeok han Seungyong Shin ga-Hyun Bae Boram Son Tae-Hyung Kim Hee Ho Park Chun Gwon Park Wooram Park Department of Integrative Biotechnology Sungkyunkwan University(SKKU)SuwonGyeonggi 16419Republic of Korea Deparment of Inteligent Precision Healthcare Convergence SKKUSuwonGyeonggi 16419Republic of Korea Department of MetaBioHealth SKKU Institute for ConvergenceSKKUSuwonGyeonggi 16419Republic of Korea Department of Bioengineering Hanyang UniversitySeoul 04763Republic of Korea Department of Integrative Engineering Chung-Ang UniversitySeoul 06974Republic of Korea Department of Biomedical Engineering SKKUSuwonGyeonggi 16419Republic of Korea Korea Institute of Science and Technology(KIST) Seoul 02792Republic of Korea

Optimum genetic delivery for modulating target genes to diseased tissue is a major obstacle for profitable gene *** nanoparticles(LNPs),considered a prospective vehicle for nucleic acid delivery,have demonstrated efficacy in human use during the COVID-19 *** study introduces a novel biomaterial-based platform,M1-polarized macrophage-derived cellular nanovesicle-coated LNPs(M1-C-LNPs),specifically engineered for a combined gene-immunotherapy approach against solid *** dual-function system of M1-C-LNPs encapsulates Bcl2-targeting siRNA within LNPs and immune-modulating cytokines within M1 macrophage-derived cellular nanovesicles(M1-NVs),effectively facilitating apoptosis in cancer cells without impacting T and NK cells,which activate the intratumoral immune response to promote granule-mediating killing for solid tumor *** retention within tumor was observed upon intratumoral administration of M1-C-LNPs,owing to the presence of adhesion molecules on M1-NVs,thereby contributing to superior tumor growth *** findings represent a promising strategy for the development of targeted and effective nanoparticle-based cancer genetic-immunotherapy,with significant implications for advancing biomaterial use in cancer therapeutics.

关键词： Genetic-immunotherapy M1 macrophage-derived cellular nanovesicles Lipid nanoparticles(LNPs) Gene therapy siRNA Cancer immunotherapy Solid tumor Tumor microenvironment(TME)

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National Soil Organic Carbon Stocks Inventories under Different Mangrove Forest Types in gabon

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Open Journal of Forestry 2024年第2期14卷 127-140页

作者： Rolf gaël Mabicka Obame Neil-yohan Musadji Jean Hervé Mve Beh Lydie-Stella Koutika Jean Aubin Ondo Farrel Nzigou Boucka Michel Mbina Mounguengui Claude Geffroy Dé partement de Phytotechnologie Institut National d&#8217Agronomie et de Biotechnologies Universit&#233 des Sciences et Techniques de Masuku Franceville Gabon Dé partement des Sciences Fondamentales de l&#8217Ing&#233nieur Institut National d&#8217Agronomie et de Biotechnologies Universit&#233 des Sciences et Techniques de Masuku Franceville Gabon Institut Agronomiques et Forestiè res Centre National des Recherches Scientifiques et Technologiques Libreville Gabon Research Centre on the Durability and the Productivity of Industrial Plantations (CRDPI) Pointe Noire Republic of Congo Laboratoire Pluridisciplinaire des Sciences &#201cole Normale Sup&#233rieure Libreville Gabon Agence gabonaise d’ &#201tude et d&#8217Observation Spatiale Immeuble les Arcades Libreville Gabon Dé partement de G&#233ologie Faculte des Sciences Universit&#233 des Sciences et Techniques de Masuku Franceville Gabon Institut de Chimie des Milieux et Maté riaux de Poitiers (IC2MP) Universit&#233 de Poitiers Poitiers France

gabonese’s estuary is an important coastal mangrove setting and soil plays a key role in mangrove carbon storage in mangrove forests. However, the spatial variation in soil organic carbon (SOC) storage remain unclear. To address this gap, determining the SOC spatial variation in gabonese’s estuarine is essential for better understanding the global carbon cycle. The present study compared soil organic carbon between northern and southern sites in different mangrove forest, Rhizophora racemosa and Avicennia germinans. The results showed that the mean SOC stocks at 1 m depth were 256.28 ± 127.29 MgC ha−1. Among the different regions, SOC in northern zone was significantly (p p yers contained higher SOC stocks (254.62 ± 128.09 MgC ha−1) than upper layers (55.42 ± 25.37 MgC ha−1). The study highlights that low deforestation rate have led to less CO2 (705.3 Mg CO2e ha−1 - 922.62 Mg CO2e ha−1) emissions than most sediment carbon-rich mangroves in the world. These results highlight the influence of soil texture and mangrove forest types on the mangrove SOC stocks. The first national comparison of soil organic carbon stocks between mangroves and upland tropical forests indicated SOC stocks were two times more in mangroves soils (51.21 ± 45.00 MgC ha−1) than primary (20.33 ± 12.7 MgC ha−1), savanna and cropland (21.71 ± 15.10 MgC ha−1). We find that mangroves in this study emit lower dioxide-carbon equivalent emissions. This study highlights the importance of national inventories of soil organic carbon and can be used as a baseline on the role of mangroves in carbon sequestration and climate change mitigation but the variation in SOC stocks indicates the need for further national data.

关键词： Mangroves Forest Soil Organic Carbon Stocks Rizophora Racemose Avicenia germinans gabon

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Anti-MMP-2 and MMP-9 activity of Salsola komarovii Iljin extract and its solvent fractions

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Asian Pacific Journal of Tropical Biomedicine 2020年第10期10卷 460-469页

作者： Jung-ha Kil Fatih Karadeniz ga Hyun yu Hojun Kim Junse Kim Jung Hwan Oh Jung Im Lee Chang-SukKong youngwan Seo Marine Biotechnology Center for Pharmaceuticals and Foods Silla UniversityBaegyang-daero 700 beon-gil 140Sasang-guBusan 46958Korea Department of Food and Nutrition College of Medical and Life SciencesSilla UniversityBaegyang-daero 700 beon-gil 140Sasang-guBusan 46958Korea Division of Marine Bioscience Korea Maritime and Ocean UniversityBusan 49112Korea

Objective: To investigate matrix metalloproteinases(MMP)-2 and MMP-9 inhibitory effect of Salsola komarovii Iljin, an edible halophyte with health beneficial ***: Salsola komarovii crude extracts(SKI), and solvent(n-hexane, 85% aq. MeOH, n-BuOH, and H2O) fractionated extracts of SKI were prepared. Gelatin zymography was carried out to observe MMP enzymatic activity. The release of the MMP enzymes was measured by enzyme-linked immunosorbent assay. Expression of MMPs in m RNA and protein level were investigated by polymerase chain reaction analysis and immunoblotting, ***: SKI and SKI fractions inhibited active MMP-2 and MMP-9 amount in the treated cell culture medium. Also, SKI suppressed the release of MMP-2 and MMP-9 from stimulated HT1080 human fibrosarcoma cells. Furthermore, SKI suppressed the m RNA and protein expression of MMP-2 and MMP-9. SKI fractions showed parallel effects except for H2O fraction which did not yield any significant MMP inhibitory effect. Among fractions, 85% aq. Me OH was the most active fraction to inhibit both the enzymatic effect and expression of MMP-2 and ***: SKI may contain potential MMP release inhibitory compounds. Salsola komarovii is a promising source of compounds against MMP and could be utilized in the development of antitumor agents.

关键词： release utilized effect

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Estimation of hydraulic parameters from pumping tests in a multiaquifer system

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Underground Space 2020年第3期5卷 210-222页

作者： Da ha gang Zheng haizuo Zhou Chaofeng Zeng Huihui Zhang School of Civil Engineering Tianjin UniversityTianjin 300072China Key Laboratory of Coast Civil Structure Safety of Ministry of Education Tianjin UniversityTianjin 300072China Hunan Provincial Key Laboratory of Geotechnical Engineering for Stability Control and Health Monitoring School of Civil EngineeringHunan University of Science and TechnologyXiangtanHunan 411201China School of Resources and Environmental Sciences Wuhan UniversityWuhan 430079China

The hydrogeologic conditions in Tianjin,China,are characterized by a system of multiple alternating aquifers and aquitards and a high groundwater ***,the groundwater system to a depth of 70 m includes one phreatic aquifer and three semiconfined aquifers(AqI–AqIII).The distribution of aquifers varies *** estimates of aquifer parameters from pumping tests are essential in geotechnical engineering *** this study,the applicability of different models for estimating hydraulic parameters in a multiaquifer system is *** avoid errors in the graphic-analytical process and to enhance the efficiency and accuracy,a hybrid algorithm,called gaLMA(a genetic algorithm(ga)combined with the Levenberg–Marquardt(LM)algorithm),is used with the Neuman and Witherspoon model and ratio *** estimated values in the second and third semiconfined aquifers(AqII and AqIII)from various solutions are compared with the measured *** validations based on the prediction of the drawdown values are performed via a three dimensional numerical simulation.

关键词： Hydraulic parameter Leaky aquifer Pumping test Optimal approach

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Numerical Identification of Nonlocal Potentials in Aggregation

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Communications in Computational Physics 2022年第8期32卷 638-670页

作者： yuchen He Sung ha Kang Wenjing Liao hao Liu yingjie Liu Institute of Natural Sciences Shanghai Jiao Tong UniversityShanghaiChina School of Mathematics Georgia Institute of TechnologyAtlantaGeorgiaGA 30332-0160USA Department of Mathematics Hong Kong Baptist UniversityHong Kong SAR

Aggregation equations are broadly used tomodel population dynamicswith nonlocal interactions,characterized by a potential in the *** paper considers the inverse problem of identifying the potential from a single noisy spatialtemporal *** identification is challenging in the presence of noise due to the instability of numerical *** propose a robust model-based technique to identify the potential by minimizing a regularized data fidelity term,and regularization is taken as the total variation and the squared Laplacian.A split Bregman method is used to solve the regularized optimization *** method is robust to noise by utilizing a Successively Denoised Differentiation *** consider additional constraints such as compact support and symmetry constraints to enhance the performance *** also apply thismethod to identify time-varying potentials and identify the interaction kernel in an agent-based *** numerical examples in one and two dimensions are included to verify the effectiveness and robustness of the proposed method.

关键词： Aggregation equation nonlocal potential PDE identification Bregman iteration operator splitting

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An Efficient Plant Regeneration System of Hydrangea bretschneideri Dipp via Stem Segments as Explants

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Phyton-International Journal of Experimental Botany 2021年第2期90卷 595-604页

作者： Si ran Wang Jia yan Bu er ha yu’e Bai Institute of Forest Tree Genetic Breeding Forestry CollegeInner Mongolia Agricultural UniversityHohhot010000China

Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden *** engineering technology has been successfully used in many plant species,but it is limited in *** we established an efficient regeneration system by using stem segments as explants for the first *** our study,the plant growth regulators(PGRs)were evaluated at the different regeneration processes,including axillary shoots regeneration and root *** found that the optimal concentration for axillary buds’induction was 2.0 mgL^(-1)6-BA and 0.5 mgL^(-1)1 IAA,its highest induction rate was 70%.Moreover,the highest axillary shoots proliferation coefficient was 10.7 on the Murashige and Skoog(MS)medium with 2.0 mgL^(-1)6-benzyladenine(BA),0.2 mgL^(-1)indole-3-butyric acid(IBA),and 1.0 mgL^(-1)gibberellin A3(ga3).The highest frequency of root induction was 80.0±0.06%by culturing the elongated shoots in 1/2 MS medium containing 0.1 mgL^(-1)*** summary,our study will provide an effective technology for large-scale propagation and important pathway for promoting the popularization and application of Hydrangea bretschneideri Dipp.

关键词： Hydrangea bretschneideri Dipp stem segments regeneration

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Ectopic expression of soybean GmKNT1 in Arabidopsis results in altered leaf morphology and flower identity

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Journal of Genetics and Genomics 2008年第7期35卷 441-449页

作者： Jun Liu Da ha Zongming Xie Chunmei Wang Huiwen Wang Wanke Zhang Jinsong Zhang Shouyi Chen National Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

Plant morphology is specified by leaves and flowers, and the shoot apical meristem （SAM） defines the architecture of plant leaves and flowers. Here, we reported the characterization of a soybean KNOX gene GmKNT1, which was highly homologous to Arabidopsis STM. The GmKNT1 was strongly expressed in roots, flowers and developing seeds. Its expression could be induced by IAA, ABA and JA, but inhibited by ga or cytokinin. Staining of the transgenic plants overexpressing GmKNT1-GUS fusion protein revealed that the GmKNT1 was mainly expressed at lobe region, SAM of young leaves, sepal and carpel, not in seed and mature leaves. Scanning electron microscopy （SEM） disclosed multiple changes in morphology of the epidermal cells and stigma. The transgenic Arabidopsis plants overexpressing the GmKNT1 showed small and lobed leaves, shortened internodes and small clustered inflorescence. The lobed leaves might result from the function of the meristems located at the boundary of the leaf. Compared with wild type plants, transgenic plants had higher expression of the SAM-related genes including the CUP, WUS, CUC1, KNAT2 and KNAT6. These results indicated that the GmKNT1 could affect multiple aspects of plant growth and development by regulation of downstream genes expression.

关键词： soybean ectopic expression GmKNT1 KNOX genes development

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柳树丛枝病感病植株内源激素含量变化研究

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陕西农业科学 2024年第8期70卷 109-112页

作者：哈琦琦孙玉洁徐奕琳李鲜花榆林学院生命科学学院陕西榆林719000

为探究植原体诱发的柳树丛枝病病株内源激素含量的变化,为柳树丛枝病的致病机理及防治提供理论依据。本研究以垂柳和旱柳为试验材料,采用LC-MS/MS法检测丛枝病病株和健株叶片中四大类13种植物激素的含量变化情况。结果表明,两种柳树病... 详细信息

为探究植原体诱发的柳树丛枝病病株内源激素含量的变化,为柳树丛枝病的致病机理及防治提供理论依据。本研究以垂柳和旱柳为试验材料,采用LC-MS/MS法检测丛枝病病株和健株叶片中四大类13种植物激素的含量变化情况。结果表明,两种柳树病株与健株相比,ABA和CTK类激素DZ、6-BA显著增加,生长素类激素NAA、IPA和ga类激素ga-3、ga-4、ga-7显著减少。柳树被植原体侵染后其内源激素含量剧烈变化,从而导致柳树出现丛枝、腋芽丛生等症状。

关键词：柳树丛枝病内源激素液相色谱串联质谱法

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ADGRV1多态性与子宫颈癌关联性研究

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实验与检验医学 2024年第3期42卷 235-240页

作者：敖梅红郑涵王艳胡双邓旭情孙仙娥李隆玉江西省妇幼保健院肿瘤科江西南昌330006 南昌大学医学院江西南昌330006

目的探讨粘附G蛋白偶联受体V1(ADGRV1)基因多态性与子宫颈癌易感性之间是否存在关联性。方法采取病例对照(1∶1)匹配研究法,分析ADGRV1基因多态性与子宫颈癌易感性是否有关,同时对子宫颈癌患者ADGRV1基因的rs59661306位点基因分布在不... 详细信息

目的探讨粘附G蛋白偶联受体V1(ADGRV1)基因多态性与子宫颈癌易感性之间是否存在关联性。方法采取病例对照(1∶1)匹配研究法,分析ADGRV1基因多态性与子宫颈癌易感性是否有关,同时对子宫颈癌患者ADGRV1基因的rs59661306位点基因分布在不同病理特征间作对比。结果ADGRV1基因的rs59661306位点携带ga及GG基因型的个体比携带AA基因型的个体患子宫颈癌的风险显著增大,相应的OR(95%CI)为4.20(1.64~10.72)和3.22(1.23~8.38)。ADGRV1基因的rs59661306位点基因分布在高危人乳头瘤病毒(HPV)感染、肿瘤家族史者、鳞状细胞癌抗原(SCC)不同值中的差异有统计学意义(Pga及GG基因型比携带AA基因型的个体更易伴高危HPV感染,相应的OR(95%CI)为13.70(2.32~80.80)和17.53(2.76~111.30)。携带AA基因型的子宫颈癌个体不易伴有家族性肿瘤史。结论ADGRV1基因的rs59661306位点基因多态性与子宫颈癌发病风险有一定相关性,在部分高危环境因素中促使子宫颈癌发生。

关键词：子宫颈癌粘附G蛋白偶联受体V1 多态性

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比色法测定蒙药铜灰体系中产生的·OH自由基

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光谱实验室 2001年第2期18卷 210-212页

作者：贺锋嘎哈森其木格斯琴内蒙古民族大学理工学院化学系内蒙古通辽市028043 包头医学院附属医院内蒙古包头市014700

根据细胞色素 C的氧化特性 ,采用比色法测定蒙药铜灰体系中产生的·OH自由基。实验证明 ,以盐酸羟胺为还原剂 ,在磷酸二氢钾缓冲溶液中测定 ,效果较好。该体系不受 β-胡萝卜素的抑制 ,仅受硫脲的抑制 ,说明蒙药铜灰可作为筛选抗· OH... 详细信息

根据细胞色素 C的氧化特性 ,采用比色法测定蒙药铜灰体系中产生的·OH自由基。实验证明 ,以盐酸羟胺为还原剂 ,在磷酸二氢钾缓冲溶液中测定 ,效果较好。该体系不受 β-胡萝卜素的抑制 ,仅受硫脲的抑制 ,说明蒙药铜灰可作为筛选抗· OH自由基的消除剂 ,并具有“燥脓、清肺、肝之热”等功能。

关键词：蒙药铜灰盐酸羟胺药物分析烃基自由基比色法

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