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The biotechnological potential of using mono-and co-cultures of Aspergillus niger van Tieghem and Trichoderma viride Pers ex *** enhance the protein content of cassava(Manihot esculenta Crantz)peels by solid substrate fermentation

引用

Studies in Fungi 2019年第1期4卷 111-122页

作者： Yafetto L Odamtten GT Adu SK ekloh e Department of Molecular Biology and Biotechnology School of Biological SciencesCollege of Agriculture and Natural SciencesUniversity of Cape CoastCape CoastGhana Department of Plant and environmental Biology School of Biological SciencesCollege of Basic and Applied SciencesUniversity of GhanaLegon-AccraGhana Department of Animal Science School of Agricultural SciencesCollege of Agriculture and Natural SciencesUniversity of Cape CoastCape CoastGhana

The application of fungal biotechnology for nutrient value addition and improvement of agro-industrial wastes is critical in the development of protein-rich feed for *** study evaluated conventional methods for protein enrichment of cassava peels by solid substrate fermentation using mono-and co-cultures of Aspergillus niger and Trichoderma *** and non-sterile cassava peels inoculated with mono-cultures of *** or *** and co-cultures of *** and *** were spontaneously fermented for 21 days at 24℃.Fermented substrates were harvested at 0,7,14,and 21 days intervals,then oven-dried at 60℃,milled in a blender and further assayed for ash,carbohydrate,crude fibre,fat,and protein *** showed that percentage increase in protein contents after 21 days incubation of sterile cassava peels were 71.02%(for mono-culture of ***)and 71.64%(for mono-culture of ***);co-culture of *** and *** yielded a 129.00%increase in *** percentage increase in protein content of the non-sterile cassava peels was 126.80%(for mono-culture of ***)and 168.34%(for mono-culture of ***)in contrast with 63.47%obtained for the co-culture of *** and *** in non-sterile cassava peels,which was attributable to possible antibiosis due to microbial *** content significantly(p≤0.05)increased for 21 days while carbohydrate,crude fibre and fat contents in both sterile and non-sterile cassava peels decreased over the same *** findings underscore the fact that protein content of cassava peels can be significantly(p≤0.05)enriched through fungal biotechnology to produce value-added feed supplement for *** practical implications of these findings are discussed.

关键词： agro-industrial wastes environmental mycoremediation fungal biotechnology nutrient-enriched feed

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