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Cultivating Pleurotus ostreatus and Pleurotus eryngii mushroom strains onagro-industrial residues in solid-state fermentation. Part II: Effect onproductivity and quality of carposomes

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Carbon Resources Conversion 2022年第1期5卷 52-60页

作者： eirini-Maria Melanouri Marianna Dedousi Panagiota Diamantopoulou Laboratory of Edible Fungi Institute of Technology of Agricultural ProductsHellenic Agricultural Organization-Demeter1 S.Venizelou Street14123 LykovrysiAttiki Greece

The potential of selected Pleurotus ostreatus and P. eryngii wild-type and commercial strains to colonize andproduce carposomes after solid-state fermentation of five substrates constituted of agro-residues namely wheatstraw (Ws), beech wood shavings (BWs), coffee residue (CR), barley and oats straw (BOs), rice bark (RB),supplemented with wheat bran was investigated. The effect of substrate composition on bioprocess feasibilitywas assessed for the different strains by quantitative (i.e., mushroom yield and Biological Efficiency-BE %) andcarposome qualitative parameters (i.e., weight, size, colour, firmness). P. ostreatus strains produced carposomesearlier than P. eryngii ones. Early fruiting formation was promoted in Ws for P. ostreatus strains, whereas forP. eryngii the lowest values of earliness were observed on BOs substrate. As for crop productivity, P. ostreatusstrain AMRL 150 provided the highest BE (>70%) in all substrates except for RB, while P. ostreatus strain AMRL144 achieved the highest yield and BE in BOs and BWs (75.30 and 64.26%, respectively). P. eryngii stainsproduced less number but heavier carposomes. The highest values of BE% for these strains were recorded on Wsand BOs substrates. The BE was further correlated to growth parameters of fungal vegetative phase. As formushroom colour, P. ostreatus were lighter than P. eryngii and BOs substrate promoted lightness in both *** P. ostreatus mushrooms were produced at CR. No differences in the whiteness and firmness were detectedamong P. eryngii mushrooms and at any substrate used. The data included in this paper showed that Pleurotusmushrooms can be cultivated on low- or zero-value agro-industrial residues of great financial and environmentalimportance towards the production of value-added food products.

关键词： Oyster mushrooms Biological efficiency Carposomes Pileus stipe Colour Texture

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Carposome productivity of Pleurotus ostreatus and Pleurotus eryngii growing on agro-industrial residues enriched with nitrogen,calcium salts and oils

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Carbon Resources Conversion 2023年第2期6卷 150-165页

作者： Marianna Dedousi eirini-Maria Melanouri Panagiota Diamantopoulou Laboratory of Edible Fungi Institute of Technology of Agricultural Products(ITAP)Hellenic Agricultural Organization-Dimitra1 S.Venizelou Str14123 LykovryssiGreece Laboratory of Food Microbiology and Biotechnology Department of Food Science and NutritionAgricultural University of Athens75 Iera Odos Str11855 AthensGreece

The suitability of the abundant agro-industrial residues wheat straw(Ws;control),barley and oats straw(BOs)and rice husk(RH),supplemented with various sources of oils(sunflower,corn oil),nitrogen(peptone,yeast extract)and calcium salts(CasO_(4)⋅2H_(2)O,CaCl2),as novel substrates in solid-state fermentation of selected Pleurotus ostreatus and *** mushrooms was *** possible effect of different additives on mycelial growth rate,biomass production and endoglucanase,laccase and lipase biosynthesis were ***,their impact on essential cultivation aspects(earliness,total mushroom yield,biological efficiency)and carposome quality parameters(weight,morphological characteristics)was *** fungi showed their highest growth rates on BOs substrates and the most positive implementation was CasO_(4)⋅2H_(2)O 6%w/w(Kr=9.58 mm/day;***,Kr=9.42 mm/day;***),while different additives led to enhancement of biomass *** species demonstrated minimal levels of endoglucanase activity,with values ranging from 0.01 to 0.42 U/g of dry weight,regardless of the substrate and the stage of *** the contrary,the maximum values of laccase activity were observed at 50%of colonization on BOs and RH,while supplementation with nitrogen and calcium sources positively affected its *** and *** cultivated on BOs supplemented with peptone at 2 and 5%w/w,synthesized significant laccase amounts,i.e.,12,165.78 and 8,624.55 U/g d.w.,*** amounts of lipase were produced,especially in substrates supplemented with sunflower 2%w/w,in quantities up to 1.42 U/g d.w.,whereas the highest lipase activity was achieved by *** on Ws supplemented with corn oil at 2%w/w,with a value of 4.25 U/g *** *** fermentation of Pleurotus species in polypropylene bags,Ws and BOs supported faster colonization and shorter earliness period than RH substrates,whereas supplementation did no

关键词： Oyster mushrooms Residues Additives Biomass Endoglucanase Laccase Lipase Weight

Probiotics: shaping the gut immunological responses

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World Journal of Gastroenterology 2024年第15期30卷 2096-2108页

作者： eirini Filidou Leonidas Kandilogiannakis Anne shrewsbury George Kolios Katerina Kotzampassi Faculty of Medicine Laboratory of PharmacologyDemocritus University of ThraceAlexandroupolis 68100Greece Department of surgery Aristotle University of ThessalonikiThessaloniki 54636Greece

Probiotics are live microorganisms exerting beneficial effects on the host’s health when administered in adequate *** the most popular and adequately studied probiotics are bacteria from the families Lactobacillaceae,Bifidobacteriaceae and *** of them have been shown,both in vitro and in vivo studies of intestinal inflammation models,to provide favorable results by means of improving the gut microbiota composition,promoting the wound healing process and shaping the immunological *** intestinal conditions,such as inflammatory bowel diseases(IBD),are characterized by an imbalance in microbiota composition,with decreased diversity,and by relapsing and persisting inflammation,which may lead to mucosal *** the results of the clinical studies investigating the effect of probiotics on patients with IBD are still controversial,it is without doubt that these microorganisms and their metabolites,now named postbiotics,have a positive influence on both the host’s microbiota and the immune system,and ultimately alter the topical tissue *** influence is achieved through three axes:(1)By dis-placement of potential pathogens via competitive exclusion;(2)by offering protection to the host through the secretion of various defensive mediators;and(3)by supplying the host with essential *** will analyze and discuss almost all the in vitro and in vivo studies of the past 2 years dealing with the possible favorable effects of certain probiotic genus on gut immunological responses,highlighting which species are the most beneficial against intestinal inflammation.

关键词： Probiotics Lactobacillaceae Bifidobacteriaceae saccharomyces Intestinal inflammation Immune responses

Posterior reversible encephalopathy syndrome following sepsis in a Crohn's disease patient: A case report

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World Journal of Clinical Cases 2016年第4期4卷 103-107页

作者： Ioannis Papaconstantinou Dionysios s Mantzos eirini Pantiora Marios K Tasoulis sofia Vassilopoulou Gerassimos Mantzaris 2~(nd) Department of surgery Aretaieio Hospital Medical School University of Athens 1~(st)Department of Neurology Eginition Hospital Medical School University of Athens 1~(st) Gastroenterology Department Evangelismos Hospital

Posterior reversible encephalopathy syndrome(PREs)is a clinico-ragiological syndrome presenting with neurological symptoms and characteristic radiologic *** occurs in the setting of various clinical conditions and requires prompt management of the causative factor for a full *** is a case report of a Crohn’s disease patient who developed PREs syndrome during a complicated post-operative *** the presence of multiple causative factors,sepsis was considered as the predominant *** prompt management,the patient recovered with no permanent neurological damage.

关键词： Crohn’s disease sepsis Posterior reversible encephalopathy syndrome Diagnosis

Diagnostic and clinical significance of antigen-specific pancreatic antibodies in inflammatory bowel diseases: A meta-analysis

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World Journal of Gastroenterology 2020年第2期26卷 246-265页

作者： Konstantinos Gkiouras Maria G Grammatikopoulou Xenophon Theodoridis eirini Pagkalidou Evangelia Chatzikyriakou Anna G Apostolidou eirini I Rigopoulou Lazaros I sakkas Dimitrios Petrou Bogdanos Department of Rheumatology and Clinical Immunology Faculty of MedicineSchool of Health SciencesUniversity of ThessalyBiopolisLarissa GR41110Greece Faculty of Medicine School of Health SciencesAristotle University of ThessalonikiUniversity CampusThessaloniki GR54124Greece Department of Nutritional sciences and Dietetics School of Health SciencesInternational Hellenic UniversitySindos CampusThessaloniki GR57400Greece Laboratory of Hygiene Social and Preventive Medicine and Medical StatisticsFaculty of MedicineSchool of Health SciencesAristotle University of ThessalonikiUniversity CampusThessaloniki GR54124Greece Laboratory of Clinical Neurophysiology AHEPA University HospitalFaculty of MedicineAristotle University of ThessalonikiUniversity CampusThessaloniki GR54124Greece Department of Medicine and Research Laboratory of Internal Medicine University Hospital of LarissaBiopolisLarissa GR41110Greece Division of Transplantation Immunology and Mucosal BiologyMRC Centre for TransplantationKing's College London Medical SchoolLondon GR41110United Kingdom

BACKGROUND Non-invasive criteria are needed for Crohn’s disease(CD)diagnosis,with several biomarkers being *** of individual diagnostic test accuracy studies assessing the diagnostic value of pancreatic autoantibodies-to-glycoprotein-2(anti-GP2)tests for the diagnosis of CD appear *** To systematically review and meta-analyze evidence on the diagnostic accuracy of anti-GP2 tests in patients with suspected/confirmed *** An electronic search was conducted on PubMed,Cochrane-CENTRAL and grey literature(CRD42019125947).The structured research question in PICPTR format was“Population”including patients with symptoms akin to CD,the“Index test”being anti-GP2 testing,the“Comparator”involved standard CD diagnosis,the“Purpose of test”being diagnostic,“Target disorder”was CD,and the“Reference standard”included standard clinical,radiological,endoscopical,and histological CD diagnostic *** was assessed with the Quality Assessment of Diagnostic Accuracy studies-2 tool and hierarchical models were employed to synthesize the *** Out of 722 studies retrieved,15 were *** studies had industry-related conflicts-of-interest,and most included healthy donors as controls(spectrum bias).For the combination of IgA and/or IgG anti-GP2 test,the summary sensitivity was 20%(95%confidence interval:10%-29%)at a median specificity of 97%.If the test was applied in 10000 suspected patients,9669 would be true negatives and in 26,the diagnosis would be *** this hypothetical cohort,the anti-GP2 would fail to produce a diagnosis for 81.3%of the positive *** summary points of sensitivity and high specificity were estimated for the IgG or IgA anti-GP2 *** results were observed when the analyses were restricted using specific cut-offs,or when ulcerative colitis patients were used as *** Anti-GP2 tests demonstrate low sensitivity and high *** results indicate that caution is required

关键词： Inflammatory bowel disease Gastrointestinal disease Evidence-based diagnosis sensitivity specificity Ulcerative colitis Conflicts of interest Metaregression Industry bias

Helicobacter pylori and autoimmune disease:Cause or bystander

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World Journal of Gastroenterology 2014年第3期20卷 613-629页

作者： Daniel s smyk Andreas L Koutsoumpas Maria G Myt-ilinaiou eirini I Rigopoulou Lazaros I sakkas Dimitrios P Bogdanos Institute of Liver studies Division of Transplantation Immunology and Mucosal BiologyKing's College HospitalSchool of MedicineKing's College London Department of Medicine Faculty of MedicineSchool of Health SciencesUniversity of Thessaly Department of Rheumatology Faculty of MedicineSchool of Health SciencesUniversity of Thessaly Cellular Immunotherapy and Molecular Immunodiagnostics Biomedical SectionCEntre for REsearch and TEchnology Hellas (CE.R.T.H.)/Institute for REsearch and Technology-THessaly (I.RE.TE.TH)

Helicobacter pylori (H. pylori) is the main cause of chronic gastritis and a major risk factor for gastric cancer. This pathogen has also been considered a potential trigger of gastric autoimmunity, and in particular of autoimmune gastritis. However, a considerable number of reports have attempted to link H. pylori infection with the development of extra-gastrointestinal autoimmune disorders, affecting organs not immediately relevant to the stomach. This review discusses the current evidence in support or against the role of H. pylori as a potential trigger of autoimmune rheumatic and skin diseases, as well as organ specific autoimmune diseases. We discuss epidemiological, serological, immunological and experimental evidence associating this pathogen with autoimmune diseases. Although over one hundred autoimmune diseases have been investigated in relation to H. pylori, we discuss a select number of papers with a larger literature base, and include sjögrens syndrome, rheumatoid arthritis, systemic lupus erythematosus, vasculitides, autoimmune skin conditions, idiopathic thrombocytopenic purpura, autoimmune thyroid disease, multiple sclerosis, neuromyelitis optica and autoimmune liver diseases. specific mention is given to those studies reporting an association of anti-H. pylori antibodies with the presence of autoimmune disease-specific clinical parameters, as well as those failing to find such associations. We also provide helpful hints for future research.

关键词： Autoimmunity Helicobacter pylori Infection Gastritis Mimicry Rheumatology

Impact of bisphosphonate treatment on bone mineral density after kidney transplant

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World Journal of Transplantation 2024年第3期14卷 102-109页

作者： Georgia Andriana Georgopoulou Marios Papasotiriou Theodoros Ntrinias eirini savvidaki Dimitrios s Goumenos Evangelos Papachristou Department of Nephrology and Kidney Transplantation University Hospital of PatrasPatras 26504Greece

BACKGROUND Mineral bone disease is associated with chronic kidney disease and persists after kidney *** treatment contributes to the patho-genesis of this *** treatments have shown positive but inde-finite *** To evaluate the effectiveness and safety of bisphosphonate treatment on post kidney transplantation bone mineral density(BMD).METHODs We included kidney transplant recipients(KTRs)whose BMD was measured after the operation but before the initiation of treatment and their BMD was measured at least one year *** also evaluated the BMD of KTRs using two valid mea-surements after transplantation who received no treatment(control group).REsULTs Out of 254 KTRs,62(39 men)were included in the *** were initiated in 35 KTRs in total(20 men),1.1±2.4 years after operation and for a period of 3.9±2.3 years while 27(19 men)received no *** improved significantly in KTRs who received bisphosphonate treatments(from-2.29±1.07 to-1.66±1.09,Pshowed a non-significant decrease in BMD after 4.2±1.4 years of follow-up after *** function was not affected by bisphosphonate *** KTRs with established osteoporosis,active treatment had a similar and significant effect on those with osteopenia or normal bone *** In this retrospective study of KTRs receiving bisphosphonate treatment,we showed that active treatment is effective in preventing bone loss irrespective of baseline BMD.

关键词： Mineral and bone disorders Chronic kidney disease Kidney transplant recipients Bisphosphonates Bone mineral density

Utilization of spent substrates and waste products of mushroom cultivation to produce new crops of Pleurotus ostreatus,Pleurotus eryngii and Agaricus bisporus

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Carbon Resources Conversion 2024年第2期7卷 50-63,F0004页

作者： Marianna Dedousi eirini-Maria Melanouri Dimitris Karayannis Eleftherios-Ikaros Kaminarides Panagiota Diamantopoulou Laboratory of Edible Fungi Institute of Technology of Agricultural Products(ITAP)Hellenic Agricultural OrganizationDimitra(ELGO-Dimitra)1 S.Venizelou str.14123 LykovryssiAttikiGreece Laboratory of Food Microbiology and Biotechnology Department of Food Science and NutritionAgricultural University of Athens75 Iera Odos Str.11855 AthensGreece Green Zin s.A.Agriculture 37008 PaliouriSourpiGreece

Five agro-industrial residues,their corresponding spent mushroom substrates(sMs),commercial fresh and spent mushroom compost of Agaricus(sMC)and Pleurotus ostreatus(sMs GZ)cultivation,Pleurotus and Agaricus waste(stipes/mishappen mushrooms)were combined and re-utilized as novel substrates for the cultivation of ***,*** and *** ***/sMC were used up to 40%(treatment 1 and 2),while 20%of mushroom waste were added in fresh and spent substrates(treatment 3 and 4).The impact of different substrates on mycelial growth rate and biomass production was ***,Pleurotus *** cultivated on the most promising substrates and essential cultivation aspects(earliness,total mushroom yield,biological efficiency-BE)and carposomes’quality parameters(weight,morphological characteristics)were *** and endoglucanase production by Pleurotus species were also determined at 50 and 100%of colonization *** species showed their fastest mycelial growth rate(up to 5 mm/day)on substrates consisted of sMC,whereas many combinations of species/substrate enhanced biomass *** sMs GZ supplementation positively affected laccase activity;in the cultures of *** and *** the highest values were 62,539 and 17,584 U/g d.w.,*** the contrary,small amounts of endoglucanase were produced(0.007 to 0.322 U/g d.w.);the greatest production was recorded for *** at full *** fermentation in bags,significant amount of total mushroom yield was produced in all substrates and those with sMs GZ supported the fastest earliness period and the highest BE for both Pleurotus *** values ranged from 54 to 133%for *** and from 53 to 121%for *** morphological characteristics,mushroom waste addition seemed to affect them *** data included in this paper support the effective re-utilization of different types of sMs and mushroom waste for fungal mass and enzymes’productio

关键词： Oyster mushroom Button mushroom spent mushroom compost stipes Misshapen mushrooms Glucosamine Laccase Endoglucanase Earliness Biological efficiency

Impact of 0.1% sodium hyaluronate and 0.2% sodium hyaluronate artificial tears on postoperative discomfort following cataract extraction surgery: a comparative study

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Eye and Vision 2019年第1期6卷 41-49页

作者： Panagiota Ntonti eirini-Kanella Panagiotopoulou Georgios Karastatiras Nektarios Breyannis sevasti Tsironi Georgios Labiris Department of Ophthalmology University Hospital of Alexandroupolis68100 DraganaAlexandroupolisGreece Department of Ophthalmology Papanikolaou General HospitalThessalonikiGreece Department of Ophthalmology Naval Hospital of AthensAthensGreece Department of Ophthalmology Athinaiki KlinikiAthensGreece

Background:Recent artificial tear preparations have provided 0.2%concentration of sodium ***,no published data exist on their potential superiority against 0.1%in alleviating dry-eye-disease symptoms in cataract extraction ***:A total of 180 patients that underwent cataract extraction surgery were randomly divided into 2 groups according to their postoperative regime:study group(sG)received fixed combination of tobramycin and dexamethasone(FCTD)quid for 3 weeks,and additionally 0.2%sodium hyaluronate provided in the COMOD®device quid for 6 *** group(CG)received fixed combination of tobramycin and dexamethasone(FCTD)quid for 3 weeks,and additionally 0.1%sodium hyaluronate provided in the COMOD®device quid for 6 *** following indexes were evaluated at 3 postoperative checkpoints:(1)surface discomfort index(sDI)which was derived by four direct 10-scale Likert-type questions that were addressed to the patient and pertained to:(a)foreign body sensation(FBs),(b)blinking discomfort(BD),(c)stinging sensation(ss),(d)tearing sensation(Ts),(2)Tear breakup time(TBUT),(3)schirmer’s test,(4)Central corneal thickness(CCT),and (4)Central Corneal sensitivity(CCs).Results:Both groups showed reduced CCs values at all postoperative examination points;however,sG participants had significantly better CCs(all psG had better TBUT than CG at the 3rd(p=0.03)and 6th examination points(p=0.04).Moreover,sG had better sDI scores at the 3rd(sDI=9.26±0.55)and 6th weeks(sDI=9.47±0.48)*** participants(p=0.03 and pspectively).Conclusion:The increased 0.2%sodium hyaluronate concentration in the artificial tears provided in the COMOD®device seems to address dry-eye-disease symptoms better in patients who underwent phacoemulsification surgery than the 0.1%*** registration:*** Identifier:NCT03705949 Oct 15,2018,retrospectively registered.

关键词： Cataract Postoperative regime Artificial tears surface discomfort index Hylocomod Hylogel sodium hyaluronate