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Enhancing tissue regeneration studies with luciferase-transgenic rats and bioluminescence imaging: A comprehensive review

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Chinese Journal of Plastic and Reconstructive Surgery 2024年第4期6卷 214-218页

作者： Poh-Ching Tan eiji kobayashi Qingfeng Li Shuangbai Zhou Department of Plastic and Reconstructive Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Department of Kidney Regenerative Medicine Jikei University School of Medicine

Bioluminescence imaging(BLI) in rodent models has revolutionized preclinical research in recent decades,enabling precise and noninvasive observation of cellular and molecular processes in vivo. Among various bioluminescent systems, the firefly luciferase-luciferin system is one of the most widely employed for in vivo cell tracking. This comprehensive review focuses on using luciferase-transgenic(Luc-Tg) rat models, known as firefly rats, in conjunction with BLI to investigate tissue regeneration and stem cell dynamics. Compared with other imaging modalities, BLI offers enhanced tissue penetration, reduced background noise, and the capacity to perform longitudinal studies with fewer animals, aligning with ethical research principles. Applications of Luc-Tg rats in fat grafting, soft tissue expansion, hair growth cycle analysis, and other skin studies are discussed,demonstrating the versatility and precision of BLI in tracking complex biological processes. Integrating advanced analytical and genome-editing techniques with BLI promises to enhance data interpretation's accuracy and efficiency. These advancements have deepened our understanding of the cellular fate and mechanisms underlying tissue regeneration, presenting promising avenues for optimizing therapeutic strategies in reconstructive surgery and regenerative medicine. Combining luciferase reporter genes and BLI is crucial to unraveling complex biological phenomena, advancing soft tissue regeneration research, and developing innovative therapeutic strategies for various medical conditions.

关键词： Firefly rat Fat grafting Luciferase Stem cells Skin expansion

In Vivo Development of Fetal Pig Kidneys in Mature Monkeys under Clinically Approved Immunosuppressant Drugs

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Engineering 2022年第3期8卷 65-73页

作者： Tsuyoshi Takamura Kenji Matsui Naoto Matsumoto Yatsumu Saito Toshinari Fujimoto Susumu Tajiri Shuichiro Yamanaka Kei Matsumoto Akimitsu kobayashi Izumi Yamamoto Hiroshi Sasaki Haruyuki Hirayama Hitomi Matsunari Kazuaki Nakano Hiroshi Nagashima Akihiko Kiyoshi Takao Kuroda Makoto Inoue Takeshi Miyawaki Takashi Yokoo eiji kobayashi Division of Nephrology and Hypertension Department of Internal MedicineThe Jikei University School of MedicineTokyo 105-8461Japan Department of Urology The Jikei University School of MedicineTokyo 105-8461Japan Department of Plastic and Reconstructive Surgery The Jikei University School of MedicineTokyo 105-8461Japan Meiji University International Institute for Bio-Resource Research Kanagawa 214-8571Japan Sumitomo Dainippon Pharma Co. Ltd.Osaka-fu 564-0053Japan Department of Kidney Regenerative Medicine The Jikei University School of MedicineTokyo 105-8461Japan

Controlling the immune response with only clinically approved immunosuppressant drugs is difficult in renal heterotra ns plantation from pigs to nonhuman ***,to the best of our knowledge,no reports exist on the use of fetal pigs as kidney *** study aimed to compare the degree of transplant rejection between neonatal and fetal kidneys,with genetically unmodified pigs as donors and cynomolgus monkeys as *** left kidneys of the recipient monkeys were removed,followed by transplantation of neonatal as well as fetal pig kidneys,which had undergone vascular anastomosis at the same site,into the *** was performed with only US Food and Drug Administration-approved *** fetal kidneys were transplanted into the omentum and paraaortic regions of cynomolgus ***,the engraftment and development of the transplanted tissues were pathologically examined by sampling over time(twice in each experiment).An acute rejection was observed after a few weeks in neonatal renal grafts with vascular ***,fetal pig kidneys were spared from rejection despite the administration of the same immunosuppressive protocol to the monkeys and the recipient blood vessels flowing into the fetal *** immunogenicity of fetal kidneys in pig-monkey renal heterotransplantation was lower than that of neonatal kidneys.

关键词： Cynomolgus monkey Pig Kidney Fetal kidney Immunosuppression

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Feasibility of large experimental animal models in testing novel therapeutic strategies for diabetes

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World Journal of Diabetes 2021年第4期12卷 306-330页

作者： Masaki Nagaya Koki Hasegawa Ayuko Uchikura Kazuaki Nakano Masahito Watanabe Kazuhiro Umeyama Hitomi Matsunari Kenji Osafune eiji kobayashi Hiromitsu Nakauchi Hiroshi Nagashima Meiji University International Institute for Bio-Resource Research Meiji UniversityKawasaki 214-8571KanagawaJapan Department of Immunology St.Marianna University School of MedicineKawasaki 261-8511KanagawaJapan Laboratory of Medical Bioengineering Department of Life SciencesSchool of AgricultureMeiji UniversityKawasaki 214-8571KanagawaJapan Research and Development PorMedTec Co.LtdKawasaki 214-0034KanagawaJapan Center for iPS Cell Research and Application(CiRA) Kyoto UniversityKyoto 606-8507KyotoJapan Department of Organ Fabrication Keio University School of MedicineShinjuku 160-8582TokyoJapan Institute for Stem Cell Biology and Regenerative Medicine Department of GeneticsStanford University School of MedicineStanford UniversityStanfordCA 94305United States Division of Stem Cell Therapy Institute of Medical ScienceThe University of TokyoMinato 108-8639TokyoJapan

Diabetes is among the top 10 causes of death in adults and caused approximately four million deaths worldwide in *** incidence and prevalence of diabetes is predicted to *** alleviate this potentially severe situation,safer and more effective therapeutics are urgently *** have long been the mainstay as preclinical models for basic research on diabetes,although they are not ideally suited for translating basic knowledge into clinical *** validate and optimize novel therapeutics for safe application in humans,an appropriate large animal model is *** animals,especially pigs, are well suited for biomedical research and share many similarities with humans,including body size,anatomical features,physiology,and ***,pigs already play an important role in translational studies,including clinical trials for *** in genetic engineering over the past few decades has facilitated the development of transgenic animals,including porcine models of *** article discusses features that attest to the attractiveness of genetically modified porcine models of diabetes for testing novel treatment strategies using recent technical advances.

关键词： Pancreatic islet Diabetes mellitus Pig Transgenic Genetic engineering Transplantation Xenotransplantation

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日本妇瘤学界对子宫颈癌腹腔镜手术的态度

中国实用妇科与产科杂志 2020年第4期36卷 324-325页

作者：小林栄仁大阪大学大学院医学部妇产科学系日本大阪565-0871

自子宫颈癌腹腔镜手术研究(laparoscopic approach to cervical cancer,LACC)研究结果发布以来,子宫颈癌腹腔镜手术的疗效出现了巨大争议。文章将从子宫颈癌腹腔镜手术在日本的发展历史,目前子宫颈癌腹腔镜广泛性全子宫切除术(LRH)在日... 详细信息

自子宫颈癌腹腔镜手术研究(laparoscopic approach to cervical cancer,LACC)研究结果发布以来,子宫颈癌腹腔镜手术的疗效出现了巨大争议。文章将从子宫颈癌腹腔镜手术在日本的发展历史,目前子宫颈癌腹腔镜广泛性全子宫切除术(LRH)在日本的应用情况,以及未来的发展三方面作介绍。建议严格把握子宫颈癌腹腔镜手术的适应证,并在手术时谨慎对待阴道切开的方式。

关键词：日本子宫颈癌腹腔镜

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Gastrointestinal stromal tumor of the ampulla of Vater:A case report

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World Journal of Gastroenterology 2014年第16期20卷 4817-4821页

作者： Masayoshi kobayashi Nobuto Hirata So Nakaji Toshiyasu Shiratori Hiroyuki Fujii eiji Ishii Department of Gastroenterology Kameda Medical CenterChiba 296-0041Japan

Gastrointestinal stromal tumors (GISTs) usually develop in the stomach and small intestine and only rarely occur at the ampulla of Vater, with only 11 cases reported in the literature. We report a case of a GIST of the ampulla of Vater. A 36-year-old, previously healthy man presented with a loss of consciousness lasting a few minutes. A gastroduodenal endoscopy revealed a submucosal tumor with central ulceration at the ampulla of Vater. The enhanced computed tomography scan revealed a smooth-outlined hypervascular solid mass (24 mm × 30 mm) in the second part of the duodenum. Neither lymphadenopathy nor metastasis was observed. Magnetic resonance cholangiopancreatography and endoscopic retrograde cholangiopancreatography showed normal bile and pancreatic ducts. Biopsies were collected from the ulcerative lesion, and the tumor was diagnosed as a GIST. A submucosal tumor with central ulceration may be a characteristic form of GISTs of the ampulla of Vater, and biopsy studies are useful for the diagnosing such tumors. The patient underwent pancreatoduodenectomy, and the operative specimen revealed a 2.2-cm GIST with 1 mitosis per 50 high-power fields. The gold standard for treatment of GISTs is surgical resection without rupture of a capsule. If technically possible, local resection may be considered. However, when the location of the lesion presents challenges, a pancreatoduodenectomy should be performed for GIST of the ampulla of Vater.

关键词： Gastrointestinal stromal tumor Ampulla of Vater Submucosal tumor Bleeding Biopsy Pancreatoduodenectomy

The Method for Optimum Estimation of COVID-19 Variant Type Virus Infection Status Analysis by the Multivariate Analysis Considering the Environmental Variability Impact in Japan

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Journal of Applied Mathematics and Physics 2022年第2期10卷 425-448页

作者： eiji Toma Yukinori kobayashi National Institute of Technology Tomakomai College Tomakomai Japan

Currently, the estimated value of the effective reproduction number (ERN), which is an index for grasping the COVID-19 infection status, is used for important planning and evaluation of infection prevention measures. Since ERN in the Sequential SIR model fluctuates in multiple dimensions due to changes in the surrounding environment, it is difficult to set the appropriate accuracy of the uncertainty region of the estimated data. The challenge in this study is to build a mathematical model of infectious disease according to the characteristics and data characteristics of the infectious disease and select an appropriate estimation method. Highly accurate quantitative research that analyzes the validity of “how infectious diseases prevail” from an academic point of view is the key to prediction and estimation in appropriate infection situation analysis. In this study, we adopted a statistical multivariate analysis method (T method) that enables evaluation and prediction of important factors related to ERN estimation and analysis of phenomena that change in real time (time series analysis). It was clarified that it is possible to estimate with higher accuracy by applying the T method to the estimated value of ERN by the current SIR mathematical model.

关键词： COVID-19 Sequential SIR Model Effective Reproduction Number Multivariate Analysis Method T-Method Regression Analysis

Factors Affecting the Health-Related Quality of Life of Community-Dwelling Elderly in Japan: A Focus on Spirituality

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Health 2017年第7期9卷 1095-1111页

作者： Minako kobayashi eiji Marui Department of Nursing Yokkaichi Nursing and Medical Care University Mie Japan Department of Health Sciences of Mind and Body Graduate School of University of Human Arts and Sciences Saitama Japan

This study aimed to explain factors affecting health-related quality of life among community-dwelling elderly individuals living in Japan. We conducted a questionnaire survey of 898 individuals at senior citizens clubs in a rural area near the Tokyo metropolitan area in 2015, and analyzed the responses of 715 who provided valid responses. The survey questions included basic attributes (e.g., age, economic affluence), state of health (e.g., whether s/he has heart disease or not), the Spirituality Rating Scale Related to Health in the Elderly (SP Health Scale, composed of, e.g., meaning and purpose of living, self-transcendence), the Abbreviated Lubben Social Network Scale, and the MOS 8-Item Short Form Health Survey (SF-8). Multiple regression analyses were performed using the physical component summary (PCS) and mental component summary (MCS) of the SF-8 as dependent variables and all others as independent variables. Factors associated with improved PCS were economic affluence and SP Health Scale (meaning and purpose of living), while the factors associated with reduced PCS were age and state of health (heart disease, gastrointestinal disease, osteoarthritis, lower back pain, knee pain, and eligible for long-term care). Meanwhile, factors associated with improved MCS were economic affluence, while those that were associated with reduced MCS were state of health (cancer and headache) and SP Health Scale (self-transcendence). These results suggest the need to understand spirituality in addition to the subjective economic situation, age, and medical condition of elderly individuals in order to improve their physical and mental health.

关键词： Health-Related QOL Spirituality Physical and Mental Health Community Dwelling Elderly

Early effects of oral administration of omeprazole and roxatidine on intragastric pH

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第1期13卷 29-34页

作者： Hiroshi IIDA Shingo KATO Yusuke SEKINO eiji SAKAI Takashi UCHIYAMA Hiroki ENDO Kunihiro HOSONO Yasunari SAKAMOTO Koji FUJITA Masato YONEDA Tomoko KOIDE Hirokazu TAKAHASHI Chikako TOKORO Ayumu GOTO Yasunobu ABE Noritoshi kobayashi Kensuke KUBOTA eiji GOTOH Shin MAEDA Atsushi NAKAJIMA Masahiko INAMORI Gastroenterology Division Yokohama City University School of Medicine Department of Medical Education Yokohama City University School of Medicine Office of Postgraduate Medical Education Yokohama City University Hospital

Objective： The ideal medication for the treatment of acid-related diseases, e.g., peptic ulcers, stress- related gastric bleeding, functional dyspepsia, and gastroesophageal reflux disease, should have a rapid onset of action to promote hemostasis and relieve the symptoms. The aim of our study was to investigate the inhibitory effects on gastric acid secretion of a single oral administration of a proton pump inhibitor, omeprazole 20 mg, and an H2-receptor antagonist, roxatidine 75 mg. Methods： Ten Heficobacterpylori-negative male subjects participated in this randomized, two-way crossover study. Intragastric pH was monitored continuously for 6 h after single oral admini- stration of omeprazole 20 mg and roxatidine 75 mg. Each administration was separated by a 7-d washout period. Results： During the 6-h study period, the average pH after administration of roxatidine was higher than that after administration of omeprazole （median： 4.45 vs. 2.65; P=0.0367）. Also during the 6-h study period, a longer duration of maintenance at pH above 2, 5, and 6 was observed after administration of roxatidine 75 mg than after administration of omeprazole 20 mg （median： 90.6% vs. 55.2%, P=-0.0284; 43.7% vs. 10.6%, P=0.0125; 40.3% vs. 3.3%, P=0.0125; respectively）. Conclusions： In Helicobacter pylori-negative healthy male subjects, oral administration of roxatidine 75 mg increased the intragastric pH more rapidly than that of omeprazole 20 mg.

关键词： Proton pump inhibitor H2-receptor antagonist Intragastric pH Omeprazole Roxatidine

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Treatment strategy for pancreatic head cancer with celiac axis stenosis in pancreaticoduodenectomy:A case report and review of literature

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World Journal of Gastroenterology 2022年第8期28卷 868-877页

作者： eiji Yoshida Yasutoshi Kimura Takuro Kyuno Ryoko Kawagishi Kei Sato Tsuyoshi Kono Takehiro Chiba Toshimoto Kimura Hitoshi Yonezawa Osamu Funato Makoto kobayashi Kenji Murakami Akinori Takagane Ichiro Takemasa Department of Surgery Hakodate Goryoukaku HospitalHakodate City 040-8611Japan Department of Surgery Surgical Oncology and ScienceSapporo Medical UniversitySapporo City 060-8543HokkaidoJapan Department of Diagnostic Radiology Hakodate Goryoukaku HospitalHakodate City 040-8611Japan

BACKGROUND During pancreaticoduodenectomy in patients with celiac axis(CA)stenosis due to compression by the median arcuate ligament(MAL),the MAL has to be divided to maintain hepatic blood flow in many ***,MAL division often fails,and success can only be determined *** overcome this problem,we performed endovascular CA stenting preoperatively,and thereafter safely performed *** present this case as a new preoperative treatment strategy that was *** SUMMARY A 77-year-old man with a diagnosis of pancreatic head cancer presented to our department for *** assessment revealed CA stenosis caused by *** performed endovascular stenting in the CA preoperatively because we knew that going into the operation without a strategy could lead to ischemic ***-antiplatelet therapy(DAPT)–which is needed when a stent is inserted–was then administered in parallel with neoadjuvant chemotherapy(NAC).This allowed us to administer DAPT for a sufficient period before the main pancreaticoduodenectomy procedure while obtaining therapeutic effects from *** stomach-preserving pancreaticoduodenectomy was *** operation did not require any unusual techniques and was performed ***,the patient progressed well,without any ischemic ***,curative resection was confirmed,and the patient had no recurrence or complications due to ischemia up to six months *** Preoperative endovascular stenting,with NAC and DAPT,is effective and safe prior to pancreaticoduodenectomy in potentially resectable pancreatic cancer.

关键词： Pancreaticoduodenectomy Celiac axis stenosis Median arcuate ligament Endovascular stenting Pancreatic head cancer Case report