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Multi-environment BSA-seq using large F3 populations is able to achieve reliable QTL mapping with high power and resolution: An experimental demonstration in rice

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The Crop Journal 2024年第2期12卷 549-557页

作者： Yan Zheng ei ei khine Khin Mar Thi ei ei Nyein Likun Huang Lihui Lin Xiaofang Xie Min Htay Wai Lin Khin Than Oo Myat Myat Moe San San Aye Weiren Wu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Fujian Provincial Key Laboratory of Crop Breeding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Department of Botany Mawlamyine UniversityMon StateMawlamyineMyanmar Department of Botany Dagon UniversityNorth DagonYangonMyanmar

Bulked-segregant analysis by deep sequencing(BSA-seq) is a widely used method for mapping QTL(quantitative trait loci) due to its simplicity, speed, cost-effectiveness, and efficiency. However, the ability of BSA-seq to detect QTL is often limited by inappropriate experimental designs, as evidenced by numerous practical studies. Most BSA-seq studies have utilized small to medium-sized populations, with F2populations being the most common choice. Nevertheless, theoretical studies have shown that using a large population with an appropriate pool size can significantly enhance the power and resolution of QTL detection in BSA-seq, with F_(3)populations offering notable advantages over F2populations. To provide an experimental demonstration, we tested the power of BSA-seq to identify QTL controlling days from sowing to heading(DTH) in a 7200-plant rice F_(3)population in two environments, with a pool size of approximately 500. Each experiment identified 34 QTL, an order of magnitude greater than reported in most BSA-seq experiments, of which 23 were detected in both experiments, with 17 of these located near41 previously reported QTL and eight cloned genes known to control DTH in rice. These results indicate that QTL mapping by BSA-seq in large F_(3)populations and multi-environment experiments can achieve high power, resolution, and reliability.

关键词： BSA-seq QTL mapping Large F3 population Multi-environment experiment Cross-validation

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应用基因编辑技术对缅甸优质稻品种波山的遗传改良

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分子植物育种 2022年第8期20卷 2607-2616页

作者： ei ei khine Khin Mar Thi ei ei Nyein 吴为人郑燕福建农林大学农学院福建省作物设计育种重点实验室福州350002 福建农林大学农学院福建农林大学作物遗传育种与综合利用教育部重点实验室福州350002 福建农林大学生命科学学院福州350002 Department of Botany Yangon UniversityYangon11041Myanmar

波山水稻是缅甸特有的一类优质稻品种,因其米饭口感好、气味香,深受缅甸人民喜爱,在东南亚享有盛名。然而,波山水稻具有植株高、生育期长、产量低的缺点,极大限制了其在缅甸的广泛种植和出口。为解决这个问题,必须对波山水稻的株高和生... 详细信息

波山水稻是缅甸特有的一类优质稻品种,因其米饭口感好、气味香,深受缅甸人民喜爱,在东南亚享有盛名。然而,波山水稻具有植株高、生育期长、产量低的缺点,极大限制了其在缅甸的广泛种植和出口。为解决这个问题,必须对波山水稻的株高和生育期性状进行遗传改良。本研究对来自缅甸的22份波山水稻品种进行遗传亲缘关系分析和表型鉴定,并以其中的一个典型的波山品种‘PSH-21’为材料,利用CRISP/Cas9基因编辑技术,对‘PSH-21’中的半矮秆基因Sd1和光周期敏感基因Ghd7进行基因敲除。结果表明敲除这两个基因之后,‘PSH-21’的株高降低了55 cm,生育期也明显缩短,而对其他农艺性状影响不大。本研究结果为缅甸波山水稻的遗传改良提供了良好的材料基础。

关键词：水稻波山生育期遗传改良基因编辑

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Multi-environment BSA-seq using large F₃ populations is able to achieve reliable QTL mapping with high power and resolution: An experimental demonstration in rice

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The Crop Journal 2024年第2期 549页

作者： Yan Zheng ei ei khine Khin Mar Thi ei ei Nyein Likun Huang Lihui Lin Xiaofang Xie Min Htay Wai Lin Khin Than Oo Myat Myat Moe San San Aye Weiren Wu

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Morphological, Structural, Thermal and Tensile Properties of High Density Polyethylene Composites Reinforced with Treated Argan Nut Shell Particles

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Journal of Bionic Engineering 2015年第1期12卷 129-141页

作者： Hamid Essabir Mounir ei Achaby ei Moukhtar Hilali Rachid Bouhfid Aboueikacem Qaiss Moroccan Foundation for Advanced Science Innovation and Research (MAScIR) Institute of Nanomaterials and Nanotechnology (NANOTECH) Laboratory of Polymer Processing Rabat Morocco National School of Applied Sciences Ibn Zohr University Laboratory Mechanics Processes Energy and Environment Agadir Morocco

High Density Polyethylene （HDPE） composites reinforced with treated bio-filler from Argan-Nut Shell （ANS） at various filler contents are prepared by extrusion and injection molding processes. The microstructures of the composites are charac- terized by Fourier Transform Infrared Spectroscopy （FTIS） and Scanning Electron Microscopy （SEM）; the thermal stability is analyzed by Thermogravimetric Analysis （TGA）, and their mechanical properties are investigated by dynamical mechanical analysis and rheological testing. The morphological and structural results indicate an improvement in adhesion between the ANS fillers and HDPE matrix upon alkali treatment. The mechanical properties of the composites show a significant increase in young＇s modulus with the addition of filler, a gain of 58% is marked compared to neat polymer, Thermal analysis reveals that the incorporation of bio-filler in polymer results in a decrease in decomposition temperatures. This research offers an ecological alternative to upgrade the valorization of abundant and unexploited Moroccan resources. In addition, the possibility of finding uses for ANS in composite manufacturing will help open new markets for what is normally considered waste or for use in low value products.

关键词： argan tree argan nut shell particles polymer composite melt extrusion mechanical properties

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Total versus quantum correlations in a two-mode Gaussian state

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Communications in Theoretical Physics 2021年第5期73卷 38-45页

作者： Jamal ei Qars Department of Physics Faculty of Applied SciencesAit-MelloulIbn Zohr UniversityAgadirMorocco EPTHE Department of PhysicsFaculty of SciencesIbn Zohr UniversityAgadirMorocco LPHE-MS Department of PhysicsFaculty of SciencesMohammed V UniversityRabatMorocco

In Li and Luo(2007 ***.A 76032327),the inequality(1/2)T≥Q was identified as a fundamental postulate for a consistent theory of quantum versus classical correlations for arbitrary measures of total T and quantum Q correlations in bipartite quantum ***,Hayden et al(2006 ***.26595)have conjectured that,in some conditions within systems endowed with infinite-dimensional Hilbert spaces,quantum correlations may dominate not only half of total correlations but total correlations ***,in a two-mode Gaussian state,quantifying T and Q respectively by the quantum mutual information I~G and the entanglement of formation(EoF)ε_(F)^(G),we verify thatε_(F)^(G),is always less than(1/2)I_(R)^(G( when I~G andε_(F)^(G) are defined via the Rényi-2 *** via the von Neumann entropy,ε_(F,V)^(G),may even dominate I_(V)^(G) itself,which partly consolidates the Hayden conjecture,and partly,provides strong evidence hinting that the origin of this counterintuitive behavior should intrinsically be related to the von Neumann entropy by which the EoFε_(F,V)^(G),is defined,rather than related to the conceptual definition of the EoFε_(F).The obtained results show that—in the special case of mixed two-mode Gaussian states—quantum entanglement can be faithfully quantified by the Gaussian Rényi-2 EoFε_(F,R)^(G),.

关键词： entanglement von Neumann and Rényi-2 entropies quantum mutual information Gaussian states spontaneous emission laser

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‘Hide-then-hit’to explain the importance of genotypic polymorphism of DNA repair genes in determining susceptibility to cancer

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Journal of Molecular Cell Biology 2011年第1期3卷 59-65页

作者： Pei-ei Wu Chen-Yang Shen Institute of Biomedical Sciences Academia SinicaTaipei 11529 Graduate Institute of Environmental Science China Medical UniversityTaichong 40402

have been suggested to account for different risk of developing *** this review article,on the basis of breast cancer formation as a model,we propose a‘hide-then-hit’hypothesis indicating the importance of escaping checkpoint surveillance for sub-optimal DNA repair variants to cause ***,only cells with subtle defects in repair capacity arising from low-penetrance variants of DNA repair genes would have the opportunity to grow and accumulate the genetic changes needed for cancer formation,without triggering cell-cycle checkpoint ***,distinct from high-penetrance alleles,these polymorphic alleles of DNA repair genes would predispose carriers to a higher risk of developing cancer but would not necessarily cause *** examine this,we simultaneously genotyped multiple SNPs of cell-cycle checkpoint genes and the DNA repair *** for the hypothesis came from observations that breast cancer risk associated with variant genotypes of DNA repair genes became more significant in the subgroups of women with specific genotypic statuses of checkpoint ***‘hide-then-hit’hypothesis is certainly needed to be confirmed by biological evidence in which a cause–effect relationship has to be ***,based on this,possible gene–gene interaction is considered to play an important role in modifying the cancer risk associated with genotypic polymorphism of DNA repair gene in different study populations.

关键词： DNA repair genotype polymorphism

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Positron Affinity, Deformation Potential and Diffusion Constant in AlxIn1-xSb Ternary Semiconductor Alloys

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Acta Metallurgica Sinica(English Letters) 2016年第7期29卷 661-667页

作者： Nour ei-Houda Fares Nadir Bouarissa Materials and Electronic Systems Laboratory University of Bordj-Bou-Arr6ridj 34000 Bordj-Bou-Arr6ridj Algeria Laboratory of Materials Physics and Its Applications University of M'sila 28000 M'sila Algeria 9 Rue K Fg des martyrs 34000 Bordj-Bou-Arreridj Algeria

The present investigation deals with the positron behaviour in Alxlnl_xSb ternary semiconductor alloys in the zinc-blende phase. The calculations are mainly based on a pseudopotential approach coupled with the independent particle model. Features such as elastic constants, electron and positron chemical potentials, positron deformation potential and positron diffusion constant are determined. Moreover, the positron affinity to InSb, A1Sb and their related ternary alloys and heterostructures is calculated. The present results are compared to experiment and found to be in reasonably good agreement. The information gathered from the present study can help in understanding the positron trapping at interfaces and precipitates and might be useful in slow-positron-beam experiments.

关键词： Semiconductor alloys Positrons Diffusion Surface and interface states Heterostructures

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Effects of γ-irradiation and Deformation Temperature on Tensile Properties of Pb-2 mass% Sb Alloy

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Journal of Iron and Steel Research International 2016年第7期23卷 733-738页

作者： Gh MOHAMMED S.ei-GAMAL Physics Department Faculty of ScienceNorthern Borders University Physics Department Faculty of EducationAin Shams University

Effects ofγ-irradiation and deformation temperature（T）on the tensile properties of Pb-2mass% Sb alloys were *** samples were annealed at 458 Kfor 2hin air,then water quenched after they wereγ-irradiated（the different doses were 0.5,1.0,1.5,and 2.0 MGy）.The tensile properties were performed using stress-strain measurements at a constant strain rate（1.2×10^（-3） s^（-1））and at different T（303-393K）.It was found that at constant dose,the fracture stress（σF）decreases while the fracture strain（εF）increases as *** particular T,σFincreases whileεFdecreases with increasing *** strain-hardening exponent（n）,which is the slope of the relation between ln（σ）and ln（ε）of the parabolic part of the stress-strain curve,was determined and its values increase as Tincreases and decrease as the dose *** value of the activation energy increases as the dose increases from 0.07 eV for un-irradiated sample to 0.1eV for the 2 MGy-irradiated *** values are in accordance with that needed for dislocation movement and ordering *** interpretation of the results was given,based on the creation of point and line defects due toγ-irradiation,and that results in a distribution of beta phase（Sb-phase）,leading to a difficulty in the movement of dislocations,so there is an increase in alloy hardness.

关键词： γ-irradiation deformation temperature tensile property Pb-2 mass% Sb alloy

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Output stabilization of distributed bilinear systems

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Control Theory and Technology 2018年第1期16卷 58-71页

作者： ei Hassan ZERRIK Lahcen EZZAKI MACS Laboratory Faculty of Sciences Moulay Ismail University Meknes 50000 Morocco

This paper studies regional stabilization of a distributed bilinear system evolving on a spatial domain Ω. Sufficient conditions for regional weak, strong and exponential stabilization are given. Also we discuss a re... 详细信息

关键词： Distributed bilinear systems stabilization regional stabilization

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Finite element analysis of chip formation and residual stresses induced by sequential cutting in side milling with microns to sub- micron uncut chip thickness and finite cutting edge radius

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Advances in Manufacturing 2015年第4期3卷 309-322页

作者： Nejah Tounsi Tahany ei-Wardany Open Manufacturing Solutions Inc. Pierrefonds QC Canada United Technologies Research Center East-Hartford CT US

In this paper, the effect of four sequential cuts in side milling of Ti6Al4V on chip formation and residual stresses （RS） are investigated using finite element method （FEM）. While the open literature is limited mainly to the studies of orthogonal sequential cutting with the constant uncut chip thickness greater than 0.01 mm, it is suggested herein to investigate not only the variable uncut chip thickness which characterises the down milling process, but also the uncut chip thickness in the sub-micron range using a finite cutting edge radius. For the resulting ductile machining regime, the characteristics of the chip mor- phology, the force profiles, the plastic deformation and temperature distributions have been analyzed. Furthermore, this study revealed that the RS should be extracted toward the area where the insert exits the workpiece in the FE simulation of the down-milling process. The simulation of a number of sequential cuts due to the consecutive engagements of the insert is required in order to capture the gradual accumulation of the RS before reaching an asymptotic convergence of the RS profile. The predicted RS are in reasonable agreement with the experimental results.

关键词： Finite element method (FEM) Down milling - Sequential cuts Ductile machining regime Chip formation Residual stresses (RS)

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