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Genomics, Pr.teomics & Bioinfor.atics 2007年第3期5卷 256-259页

作者： edward M.smith Jack Littr.ll Michael Olivier Human and Molecular.Genetics Center Medical College of WisconsinMilwaukeeWI 53226USA.

High-thr.ughput SNP genotyping platfor.s use automated genotype calling algor.thms to assign genotypes. While these algor.thms wor. efficiently for.individual platfor.s, they ar. not compatible with other.platfor.s, and have individual biases that r.sult in missed genotype calls. Her. we pr.sent data on the use of a second complementar. SNP genotype cluster.ng algor.thm. The algor.thm was or.ginally designed for.individual fluor.scent SNP genotyping assays, and has been optimized to per.it the cluster.ng of lar.e datasets gener.ted fr.m custom-designed Affymetr.x SNP panels. In an analysis of data fr.m a 3K ar.ay genotyped on 1,560 samples, the additional analysis incr.ased the over.ll number.of genotypes by over.45,000, significantly impr.ving the completeness of the exper.mental data. This analysis suggests that the use of multiple genotype calling algor.thms may be advisable in high-thr.ughput SNP genotyping exper.ments. The softwar. is wr.tten in Per. and is available fr.m the cor.esponding author.

关键词： cluster.ng SNP genotyping algor.thm

婴幼儿卒中的处理：来自美国心脏协会卒中委员会和青少年心血管病委员会专门写作组的科学声明

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国际脑血管病杂志 2008年第11期16卷 801-846页

作者： E.Steve r.ach() Mer.dith r. Golomb() r.ber. Adams() Jose Biller.) Stephen Daniels() Gabr.elle deVeber.) Donna Fer.ier.() Blaise V. Jones() Fenella J. Kir.ham() r. Michael Scott() edward r. smith() 刘丽芳(译) 王胜男(译) 王伟(译) 林镇洲(译) 朱佳佳(译) 潘速跃(译) 不详南方医科大学南方医院神经内科广州500515

目的：本声明旨在回顾有关儿童卒中的文献，为最佳的诊断和治疗提供推荐意见，供负责诊断和治疗婴儿、儿童和青少年脑血管病的医生使用。方法：写作组成员由美国心脏协会卒中委员会科学声明监督委员会任命，由多个不同领域的专家组成。... 详细信息

目的：本声明旨在回顾有关儿童卒中的文献，为最佳的诊断和治疗提供推荐意见，供负责诊断和治疗婴儿、儿童和青少年脑血管病的医生使用。方法：写作组成员由美国心脏协会卒中委员会科学声明监督委员会任命，由多个不同领域的专家组成。应用美国心脏协会卒中委员会的证据分级标准对每条推荐意见进行分级。在经过小组成员审查后，草稿由4位同行评议专家和卒中委员会指导委员会成员审阅，并由美国心脏协会科学咨询和协调委员会批准。我们预计该声明需在4年内进行更新。结果：为镰状细胞病、烟雾病、颈一脑动脉夹层分离和心源性栓塞引起的缺血性卒中的预防提供循证推荐，同时为出血性卒中的评价和处理提供推荐意见。提出儿童使用肝素和华法林的剂量方案。另外，还为围产期卒中和儿童脑静脉窦血栓形成的评价和处理提供推荐。

关键词：美国心脏协会科学声明儿童卒中

Acceler.ting mater.als discover. using ar.ificial intelligence, high per.or.ance computing and r.botics

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npj Computational Mater.als 2022年第1期8卷 767-775页

作者： edward O.Pyzer.Knapp Jed W.Piter. Peter.W.J.Staar.Seiji Takeda Teodor. Laino Daniel P.Sander. James Sexton John r.smith Alessandr. Cur.oni IBM r.sear.h Eur.pe-Dar.sbur. DaresburyUK IBM Almaden r.sear.h Centr. San JoseCAUSA IBM r.sear.h Eur.pe Zur.ch RüschlikonSwitzerland IBM r.sear.h Tokyo TokyoJapan IBM Thomas J.Watson r.sear.h Centr. Yorktown HeightsNYUSA

New tools enable new ways of wor.ing,and mater.als science is no *** mater.als discover.,tr.ditional manual,ser.al,and human-intensive wor. is being augmented by automated,par.llel,and iter.tive pr.cesses dr.ven by Ar.ificial Intelligence (AI),simulation and exper.mental *** this per.pective,we descr.be how these new capabilities enable the acceler.tion and enr.chment of each stage of the discover. *** show,using the example of the development of a novel chemically amplified photor.sist,how these technologies’ impacts ar. amplified when they ar. used in concer. with each other.as power.ul,heter.geneous wor.flows.

关键词： ar.ificial computing enable

Plastic multilayer.d closur. ver.us or.hopedic sur.eon closur. after.spinal instr.mentation in pediatr.c neur.muscular.scoliosis

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Wor.d Jour.al of Pediatr.c Sur.er. 2023年第1期6卷 30-35页

作者： Ekene U Ezeokoli Poor.ima L Tamma Ner.tan Bor.ci Ifeoma Inneh edward P Buchanan Br.an G smith Depar.ment of Or.hopedic Sur.er. Texas Children's HospitalHoustonTexasUSA Baylor.College of Medicine HoustonTexasUSA Depar.ment of Plastic Sur.er. Texas Children's HospitalHoustonTexasUSA Depar.ment of Or.hopedic Sur.er. Baylor College of MedicineHoustonTexasUSA

Objective To compar. wound complication r.tes between or.hopedic closur.(OC)and plastic multilayer.d closur.(PMC)in patients under.oing pr.mar. poster.or.spinal fusion for.neur.muscular.scoliosis(NMS).We hypothesize that multilayer.d closur. will be associated with better.postoper.tive *** We collected data on pediatr.c patients diagnosed with NMS who under.ent fir.t time spinal instr.mentation between 1 Januar. 2018 and 31 May *** demogr.phics,length of sur.er.,spinal levels fused and oper.tive var.ables,wound complication r.te,tr.atments,and need for.wound washout wer. r.viewed in depth and *** In total,86 patients wer. r.viewed:46 with OC and 40 with *** was a significant incr.ase in oper.ting r.om(Or.time with PMC compar.d with OC(6.7±1.2 vs 7.3±1.3,p=0.016).Ther. was no differ.nce in complication r.te,mean postoper.tive day of complication or.unplanned r.tur. to the Or.for.OC and PMC,*** was a slightly significant incr.ase in the number.of patients going home with a dr.in in the PMC cohor. compar.d with the OC cohor.(2.1%vs 15%,p=0.046).Conclusions PMC demonstr.ted longer.Or.times than OC and did not demonstr.te a statistically significant r.duction in wound complications or.unplanned r.tur.s to the ***,other.studies have demonstr.ted statistical and clinical significance with these *** pr.gr.ms should r.view inter.al patient volumes and outcomes for.spinal fusion in NMS patients and consider.if PMC after.spinal fusions in pediatr.c patients with NMS or.other.scoliosis subtypes is an appr.pr.ate option in their.institution to minimize postoper.tive wound complications.

关键词： wound pediatr.c closur.

Pitfalls in inter.r.tation of shallow seismic data

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Applied Geophysics 2012年第1期9卷 87-94,117页

作者： Ahmed Ismail edward smith Andr.w Phillips Andr.w Stumpf Illinois State Geological Sur.ey Division Univer.ity of Illinois Ur.ana-Champaign National r.sear.h Institute of Astr.nomy and Geophysics

High-r.solution shallow seismic methods ar. the most widely used geophysical methods in near.sur.ace char.cter.zation. However. in many cases inter.r.ting the seismic images can be misleading. In this ar.icle, we pr.sent thr.e case studies wher. r.sults fr.m P-wave seismic r.flection, SH-wave seismic r.flection, and multi-channel analysis of sur.ace wave （MASW） sur.eys wer. incor.ectly inter.r.ted because of inadequate constr.ints on either.the sur.eyed sites sur.ace or.subsur.ace conditions. A P-wave r.flection sur.ey featur. was fir.t inter.r.ted as a shallow fault zone but it was later.deter.ined to r.sult fr.m a high level of backgr.und noise as the acquisition passed thr.ugh a r.ad inter.ection. A SH-wave seismic r.flection sur.ey featur. was inter.r.ted to be a r.ver.e dip-slip fault but tar.eted dr.lling showed it was deep local er.sion into the bedr.ck sur.ace. Finally, in an MASW sur.ey, a steeply dipping featur. was fir.t inter.r.ted as a bedr.ck valley. However. later.explor.tor. dr.lling showed the featur. to be a shallow layer.of ver. soft lake sediment that sever.ly damped most of the applied sur.ace wave fr.quency band. Although initial inter.r.tations wer. incor.ect, they stimulated discussions among geophysicists and geologists and under.cor.d the need for.meaningful cooper.tion and discour.e between the scientists befor., dur.ng, and after.geophysical data acquisition.

关键词： Seismic r.flection multi-channel analysis of sur.ace wave inter.r.tation pitfalls

Osteogenesis imper.ecta mutations in plastin 3 lead to impair.d calcium r.gulation of actin bundling

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Bone r.sear.h 2020年第3期8卷 320-332页

作者： Chr.stopher.L.Schwebach Elena Kudr.ashova Weili Zheng Matthew Or.har. Har.er.smith Lucas A.r.nyan edward H.Egelman Dmitr. S.Kudr.ashov Depar.ment of Chemistr. and Biochemistr. The Ohio State UniversityColumbusOH 43210USA Molecular.Cellular.and Developmental Biology gr.duate pr.gr.m The Ohio State UniversityColumbusOH 43210USA Depar.ment of Biochemistr. and Molecular.Genetics University of VirginiaCharlottesvilleVA 22908USA Biophysics gr.duate pr.gr.m The Ohio State UniversityColumbusOH 43210USA

Mutations in actin-bundling pr.tein plastin 3(PLS3)emer.ed as a cause of congenital osteopor.sis,but neither.the r.le of PLS3 in bone development nor.the mechanisms under.ying PLS3-dependent osteopor.sis ar. *** the over.20 identified osteopor.sis-linked PLS3 mutations,we investigated all five that ar. expected to pr.duce full-length *** of the mutations distor.ed an actin-binding loop in the second actin-binding domain of PLS3 and abolished F-actin bundling as r.vealed by cr.o-EM r.constr.ction and pr.tein inter.ction ***,the r.maining four.mutants fully r.tained F-actin bundling ***,they displayed defects in Ca2+sensitivity:two of the mutants lost the ability to be inhibited by Ca2+;while the other.two became hyper.ensitive to *** gr.up of the mutants with similar.biochemical pr.per.ies showed highly char.cter.stic cellular.***-type PLS3 was distr.buted between lamellipodia and focal *** str.king contr.st,the Ca2+-hyposensitive mutants wer. not found at the leading edge but localized exclusively at focal adhesions/str.ss fiber.,which displayed r.infor.ed ***,the Ca2+-hyper.ensitive PLS3 mutants wer. r.str.cted to lamellipodia,while chelation of Ca2+caused their.r.distr.bution to focal ***,the bundling-deficient mutant failed to co-localize with any F-actin str.ctur.s in cells despite a pr.ser.ed F-actin binding thr.ugh a non-mutation-bear.ng actin-binding *** findings r.vealed that sever. osteopor.sis can be caused by a mutational disr.ption of the Ca2+-contr.lled PLS3’s cycling between adhesion complexes and the leading *** of the str.ctur.l,biochemical,and cell biology insights enabled us to pr.pose a molecular.mechanism of plastin activity r.gulation by Ca2+.

关键词： cycling impair.d str.king

Str.ctur.l Design and Optimization of Composite Blades for.a Low Weight r.tor

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Structural Design and Optimization of Composite Blades for a...

Char.cter.zation of the Expr.ssion of the ATP-Gated P2X7 r.ceptor.Following Status Epilepticus and dur.ng Epilepsy Using a P2X7-EGFP r.por.er.Mouse

第二届旋翼飞行器技术基础研究国际学术会议

作者： edward smith Depar.ment of Aer.space Engineer.ng The Pennsylvania State Univer.ity Univer.ity Par. PA 16803

＜正＞A design methodology and analysis pr.cedur. of composite r.tor.blades is developed. The design methodology is applied in the low weight r.tor.blade design to suppor. NASA Heavy Lift r.tor.Systems Investigation. The r.sulted blade r.aches the tar.eted weight r.duction and satisfies the stiffness, str.ngth and per.or.ance r.quir.ments fr.m NASA r.tor.compr.hensive analysis. Sever.l blade design issues ar. also discussed.

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Neur.science Bulletin 2020年第11期36卷 1242-1258页

作者： James Mor.an Mar.ana Alves Gior.ia Conte Aida Menendez-Mendez Laur. de Diego-Gar.ia Gioacchino de Leo edward Beamer.Jonathon smith Annette Nicke Tobias Engel Depar.ment of Physiology and Medical Physics Royal College of Surgeons in IrelandDublin D02 YN77Ireland Futur.Neur. Science Foundation Ireland Research Centre for Chronic and Rare Neurological DiseasesRoyal College of Surgeons in IrelandDublin D02 YN77Ireland Walther.Str.ub Institute of Phar.acology and Toxicology Faculty of MedicineLudwig-Maximilians-Universitat Miinchen80336 MunichGermany

Mounting evidence suggests that the ATP-gated P2X7 r.ceptor.contr.butes to incr.ased hyper.xcitability in the *** incr.ased expr.ssion of P2X7 in the hippocampus and cor.ex following status epilepticus and dur.ng epilepsy has been r.peatedly demonstr.ted,the cell type-specific expr.ssion of P2X7 and its expr.ssion in extr.-hippocampal br.in str.ctur.s r.mains incompletely *** this study,P2X7 expr.ssion was visualized by using a tr.nsgenic mouse model over.xpr.ssing P2X7 fused to the fluor.scent pr.tein *** r.sults showed incr.ased P2X7-EGFP expr.ssion after.status epilepticus induced by intr.-amygdala kainic acid and dur.ng epilepsy in differ.nt br.in r.gions including the hippocampus,cor.ex,str.atum,thalamus and cer.bellum,and this was most evident in micr.glia and *** of P2X7-EGFP with cell type-specific mar.er. was not detected in neur.ns or.*** data suggest that P2X7 activation is a common pathological hallmar. acr.ss differ.nt br.in str.ctur.s,possibly contr.buting to br.in inflammation and neur.degener.tion following acute seizur.s and dur.ng epilepsy.

关键词： Pur.ner.ic signaling P2X7 Gr.en fluor.scence pr.tein Status epilepticus Epilepsy Cell typespecific expr.ssion

r.pid, simple, low-cost smar.phone-based fluor.scence detection of Escher.chia coli

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Inter.ational Jour.al of Agr.cultur.l and Biological Engineer.ng 2021年第3期14卷 189-193页

作者： Dante r.jas-Bar.oza edward Par. r.lfe Sassenfeld Jer.my Winder.Geoffr.y B.smith Delia Valles-r.salles Efr.n Delgado Young Ho Par. Family and Consumer.Science Depar.ment New Mexico State UniversityLas CrucesNM 883003USA Biology Depar.ment University of PennsylvaniaPhiladelphiaPA 19104USA Electr.nics&Computer.Engineer.ng Technology New Mexico State UniversityLas CrucesNM 88003USA Biology Depar.ment New Mexico State UniversityLas CrucesNM 88003USA Industr.al Engineer.ng Depar.ment New Mexico State UniversityLas CrucesNM 88003USA Mechanical Engineer.ng Depar.ment New Mexico State UniversityLas CrucesNM 88003USA

Food and water.or.e diseases pose consider.ble public health thr.ats even in highly industr.alized par.s of the *** of these pathogens in food can be Escher.chia coli O157:H7,Salmonella sp.,and Lister.a ***,r.liable detection of pathogens mitigates ser.ous health pr.blems and economic losses due to outbr.aks and r.bust tests safeguar. the food *** this study,a smar.phone-based appar.tus was employed to demonstr.te quantitative detection of *** validate the applicability of the pr.sent smar.phone-based fluor.scence device,r.A was extr.cted fr.m the *** K-12 str.in and amplified using two differ.nt pr.mer.(dnaK and r.oA)via quantitative polymer.se chain r.action(qPCr..Ser.al dilutions of r.A fr.m 10 to 0.0001 ng/μL wer. pr.par.d at the star. of the PCr.amplification and the PCr.pr.ducts wer. detected by CYBr.Gr.en1-based *** a pr.of-of-concept test for.the smar.phone system,samples fr.m these PCr.pr.ducts wer. then *** detection system employed a novel algor.thm to analyze fluor.scence signals and r.ad changes in *** DNA *** cor.elations between the fluor.scence per.entage and DNA concentr.tions wer. r.0.945 for.the dnaK pr.mer.and r.0.893 for.the r.oA pr.mer.*** this new fluor.scent analysis technique r.sulted in compar.ble accur.cy to the r.al-time PCr.fluor.scent signal *** key innovation of this appr.ach was to combine efficient image pr.cessing encoded into a smar.phone application with a low-cost 3-D pr.nted device that allowed quantification of bacter.al nucleic acid.

关键词： smar.phone fluor.scence E.coli low-cost 3D-pr.nt

Lack of <i>r.l</i>1/p107 Effects on Cell Pr.lifer.tion and Matur.tion in the Inner.Ear

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Jour.al of Behavior.l and Br.in Science 2013年第7期3卷 534-555页

作者： Sonia M. r.cha-Sanchez Laur. r. Scheetz Sabr.na Siddiqi Michael W. Weston Lynette M. smith Kate Dempsey Hesham Ali JoAnn McGee edward J. Walsh College of Infor.ation Science and Technology University of Nebraska at Omaha Omaha USA College of Public Health University of Nebraska Medical Center Omaha USA Depar.ment of Or.l Biology Creighton University School of Dentistry Omaha USA Developmental Auditor. Physiology Labor.tor. Boys Town National Research Hospital Omaha USA

Loss of postnatal mammalian auditor. hair.cells (HCs) is ir.ever.ible. Ear.ier.studies have highlighted the impor.ance of the r.tinoblastoma family of pr.teins (pr.s) (i.e., r.1, r.l1/p107, and r.l2/p130) in the auditor. cells’ pr.lifer.tion and emphasized our.lack of infor.ation on their.specific r.les in the auditor. system. We have pr.viously demonstr.ted that lack of r.l2/p130 moder.tely affects HCs’ and suppor.ing cells’ (SCs) pr.lifer.tion. Her., we pr.sent evidence suppor.ing multiple r.les for.r.l1/p107 inthe developing and matur. mouse or.an of Cor.i (OC). Like other.pr.s, r.l1/p107 is expr.ssed in the OC, par.icular.y in the Hensen’s and Deiter.’ cells. Mor.over. r.l1/p107 impacts matur.tion and postmitotic quiescence of HCs and SCs, as evidenced by enhanced number. of these cells and the pr.sence of dividing cells in the postnatal r.l1/p107-/-OC. These findings wer. fur.her.suppor.ed by micr.ar.ay and bioinfor.atics analyses, suggesting downr.gulation of sever.l bHLH molecules, as well as activation of the Notch/Hes/Hey signaling pathway in homozygous r.l1/p107 mutant mice. Physiological assessments and detection of ectopic HC mar.er.expr.ssion in postnatal spir.l ganglion neur.ns (SGNs) pr.vided evidence for.incomplete cell matur.tion and differ.ntiation in r.l1/p107﹣/﹣OC. Collectively, the pr.sent study highlights an impor.ant r.le for.r.l1/p107 inOC cell differ.ntiation and matur.tion, which is distinct fr.m other.pr.s.

关键词： r.l1/p107 Inner Ear Pr.lifer.tion Differ.ntiation Hair Cells Suppor.ing Cells