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Advances in Biological Chemistry 2022年第6期12卷 207-227页

作者： Luana Cristina da silva Ramos André Henrique Furtado Torres Letícia damas Leão dalcin Katleyn Polizeli Galvão silva Luana Gabriela silva de Freitas Mahmi Fujimori danny Laura Gomes Fagundes eduardo Luzia França Adenilda Cristina Honorio França Universidade Federal de Mato Grosso. Barra do Garç as Brazil

The study aimed to develop and characterize a microemulsified system based on cotton oil and verify its effect on superoxide release anion and microbicidal activity by human peripheral blood cells. Microemulsions were formulated using distilled water, degummed cotton oil, Span 80 (SP), Tween 80 (TW), and 1-butanol (BT). The pseudo-ternary diagram delimited ME regions, and the points were pre-selected. The physical-chemical and rheological characterization of the microemulsions was carried out. The ME activity on the interactions between leukocytes and bacteria was analyzed by superoxide release, phagocytosis, and microbicidal activity. The developed formulation was classified as Oil/Water, with an average pH of 5.76, and the viscosity showed resistance to temperature changes. The rheological model of the Power Law classified the microemulsion as a non-Newtonian fluid with pseudoplastic characteristics. The cell viability of cotton oil microemulsion was greater than 90%. There was an increase in the superoxide release by MN phagocytes when treated with cotton oil microemulsion. The cotton oil microemulsion increased phagocytosis and microbicidal activity. The present study suggests that cotton oil is an alternative biomaterial for therapeutic applications, especially in treating infections.

关键词： Cotton Oil Microemulsion Phagocytosis Infection Human Phagocytes

Fishermen and Territorial Trends in the Brazilian Fisheries Policies

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International Journal of Geosciences 2015年第4期6卷 339-349页

作者： Christian Nunes da silva eduardo Schiavone Cardoso Federal University of Pará (UFPA) Belém Brazil Federal University on Santa Maria (UFSM) Santa Maria Brazil

The fishing activity has been known for centuries by man and is configured as an important source of livelihood and income for many families of Brazilian fishermen. This article tries to do a brief contextualization of fishing in Brazil, as well as discuss the notion of territoriality of sector policies. The trend shown for the future is the need for a planning/mapping of operation areas of productive agents in the country, so that fish stocks can be managed more appropriately. One of the proposals that comes with acceptance and is already being applied in some municipalities is fishing agreements, using co-management mechanisms for planning and use of fisheries resources.

关键词： Fishing and Fishermen Colony of Fishermen Brazil Agreements

Domain adversarial training for classification of cracking in images of concrete surfaces

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AI in Civil Engineering 2022年第1期1卷 119-132页

作者： Bruno oliveira Santos Jónatas Valença João P.Costeira eduardo Julio CERIS Instituto Superior TécnicoUniversity of LisbonLisbonPortugal ISR Instituto Superior TécnicoUniversity of LisbonLisbonPortugal

The development of automatic methods to recognize cracks in surfaces of concrete has been under focus in recent years,firstly through computer vision methods and more recently focusing on convolutional neural networks that are delivering promising *** are still persisting in crack recognition,namely due to the confusion added by the myriad of elements commonly found on concrete *** robustness of these methods would deal with these elements if access to correspondingly heterogeneous datasets was *** so,this would be a cumbersome methodology,since training would be needed for each particular case and models would be case ***,efforts from the scientific community are focusing on generalizing neural network models to achieve high per-formance in images from different domains,slightly different from those in which they were effectively *** generalization of networks can be achieved by domain adaptation techniques at the training *** adapta-tion enables finding a feature space in which features from both domains are invariant,and thus,classes become *** work presented here proposes the da-Crack method,which is a domain adversarial training method,to generalize a neural network for recognizing cracks in images of concrete *** domain adversarial method uses a convolutional extractor followed by a classifier and a discriminator,and relies on two datasets:a source labeled dataset and a target unlabeled small *** classifier is responsible for the classification of images randomly chosen,while the discriminator is dedicated to uncovering to which dataset each image *** from the discriminator reverses the gradient used to update the *** enables fighting the convergence promoted by the updating backpropagated from the classifier,and thus generalizing the extractor enabling it for crack recognition of images from both source and target *** show that

关键词： da-Crack method Domain-adaptation Adversarial training network Crack detection Concrete surfaces Computer vision

Bijections between Lattice Paths and Plane Partitions

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Open Journal of Discrete Mathematics 2011年第3期1卷 108-115页

作者： Mateus Alegri eduardo Henrique de Mattos Brietzke José Plínio de oliveira Santos Robson da silva 不详

By using lattice paths in the three-dimensional space we obtain bijectively an interpretation for the overpartitions of a positive integer n in terms of a set of plane partitions of n . We also exhibit two bijections ... 详细信息

关键词： Lattice Paths Plane Partitions Partitions q-Analog.

Current advances in solid free-form techniques for osteochondral tissue engineering

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Bio-Design and Manufacturing 2018年第3期1卷 171-181页

作者： Joao Bebiano Costa Joana silva-Correia Rui Luis Reis Joaquim Miguel oliveira B's Research Group I3Bs - Research Institute onBiomaterials Biodegradables and Biomimetics University ofMinho Headquarters of the European Institute of Excellenceon Tissue Engineering and Regenerative Medicine AveParkParque de Ciencia e Tecnologia Zona Industrial da Gandra4805-017 Barco Guimaraes Portugal ICVS/3B's-PT Government Associate Laboratory Braga Guimarfies Portugal The Discoveries Centre for Regenerative and PrecisionMedicine Headquarters at University of MinhoAvepark 4805-017 Barco Guimaraes Portugal

Osteochondral (OC) lesions are characterized by defects in two different zones, the cartilage region and subchondral bone region. These lesions are frequently associated with mechanical instability, as well as osteoarthritic degenerative changes in the knee. The lack of spontaneous healing and the drawbacks of the current treatments have increased the attention from the scientific community to this issue. Different tissue engineering approaches have been attempted using different polymers and different scaffolds’ processing. However, the current conventional techniques do not allow the full control over scaffold fabrication, and in this type of approaches, the tuning ability is the key to success in tissue regeneration. In this sense, the researchers have placed their efforts in the development of solid free-form (SFF) techniques. These techniques allow tuning different properties at the micro–macro scale, creating scaffolds with appropriate features for OC tissue engineering. In this review, it is discussed the current SFF techniques used in OC tissue engineering and presented their promising results and current challenges.

关键词：软骨病变生物技术生物科学发展现状

Combinatorial therapies for spinal cord injury: strategies to induce regeneration

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Neural Regeneration Research 2019年第1期14卷 69-71页

作者： eduardo D.Gomes Nuno A.silva António J. Salgado Life and Health Sciences Research Institute (ICVS) School of Medicine University of Minho Campus de Gualtar ICVS/3B’s–PT Government Associate Laboratory

Spinal cord injury(SCI)is a condition without treatment,mainly characterized by the loss of motor and sensory function below the level of *** is accompanied by several complications such as cardiac and respiratory compromise,and often patients present psychological ailments associated with the drastic alteration of their normal *** pathophysiology derives from a massive damage to the spinal cord tissue,which is propagated

关键词：损害绳索新生治疗感觉功能生活方式星形细胞并发症

Physiological Effect of Kinetin on the Photosynthetic Apparatus and Antioxidant Enzymes Activities During Production of Anthurium

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Horticultural Plant Journal 2018年第5期4卷 182-192页

作者： de MOURA Fabiana B. VIEIRA Marcos R. da S. SIMOES Adriano do N. FERREIRA-silva Sérgio L. de SOUZA Carlos A.V. de SOUZA eduardo S. da ROCHA Alexandre T. da silva Luzia F. JUNIOR Miguel A. Faculty of Agronomy Federal University of Pará Postgraduate Program in Plant Production Serra Talhada Academic UnitFederal Rural University of Pernambuco Department of Production Vegetable Unidade Academica de GaranhusFederal Rural University of Pernambuco

The results observed in the literature raise the hypothesis according to which cytokinin plays important roles in photosynthetic metabolisms and antioxidant enzymes. Thus, the study aimed to evaluate the effect of foliar application of the isolated cytokinin kinetin at the production cycle, seeking to analyze its effects on enzyme activity and photosynthetic parameters. The plants treated with CK presented reduction of leaf CO_2 assimilation rate(Pn) and stomatal conductance(Gs), while that transpiration rate(Tr) was unaffected. The internal CO_2 concentrations decreased with the increase in cytokinin levels, but were maintained under CK 50 mg·L^(-1). The plants treated with CK 75 mg·L^(-1) was verified higher carboxylation efficiency(Pn/Ci), which was associated to values of CO_2 assimilation and transpiration unaltered. Apparent electron transport rate showed variations in the concentration of 25 mg·L^(-1). Considering the study of enzyme activity, on the other hand, it cannot be stated that kinetin has an effective action in delaying oxidative damage. It presents mixed results, since an efficiency in the application of cytokinin was not observed, presenting induction levels of ascorbate peroxidase activity. Thus, further research is needed to determine more precisely the effects of kinetin on gas exchange and antioxidant enzymes in anthurium plants.

关键词： anthurium kinetin photosynthesis senescence plant hormone oxidative stress

Pediatric multisystem inflammatory syndrome associated with COVID-19: Insights in pathogenesis and clinical management

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World Journal of Virology 2023年第3期12卷 193-203页

作者： Marcel silva Luz Fabian Fellipe Bueno Lemos Samuel Luca Rocha Pinheiro Hanna Santos Marques Luís Guilherme de oliveira silva Mariana Santos Calmon Karolaine da Costa Evangelista Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Campus Vitória da Conquista Universidade Estadual do Sudoeste da BahiaVitória da Conquista 45029-094BahiaBrazil

The pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection has been a major challenge to be faced in recent *** adults suffered the highest morbidity and mortality rates of coronavirus disease 2019,children were thought to be exclusively asymptomatic or to present with mild ***,around April 2020,there was an outbreak of a new clinical syndrome related to SARS-CoV-2 in children-multisystemic inflam-matory syndrome in children(MIS-C)-which comprises a severe and uncon-trolled hyperinflammatory response with multiorgan *** Centers for Disease Control and Prevention considers a suspected case of MIS-C an individual aged2)organ involvement,no alternative plausible diagnoses,and positive for recent SARS-CoV-2 *** its severity,there are no definitive disease management guidelines for this ***,the complex pathogenesis of MIS-C is still not completely understood,although it seems to rely upon immune ***,in this study,we aim to bring together current evidence regarding the pathogenic mechanisms of MIS-C,clinical picture and management,in order to provide insights for clinical practice and implications for future research directions.

关键词： Pediatric multisystem inflammatory disease COVID-19 related Multisystem inflammatory syndrome in children COVID-19 SARS-CoV-2 Etiology Disease management

Role of nickel-regulated small RNA in modulation of Helicobacter pylori virulence factors

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World Journal of Clinical Cases 2022年第31期10卷 11283-11291页

作者： Fabrício Freire de Melo Hanna Santos Marques Fabian Fellipe Bueno Lemos Marcel silva Luz Samuel Luca Rocha Pinheiro Lorena Sousa de Carvalho Cláudio Lima Souza Márcio Vasconcelos oliveira Institution Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094Brazil Campus Vitória da Conquista Universidade Estadual do Sudoeste da BahiaVitória da Conquista 45083-900Brazil

Helicobacter pylori(***)is a Gram-negative bacterium that infects about half of the world's *** infection prevails by several mechanisms of adaptation of the bacteria and by its virulence factors including the cytotoxin associated antigen A(CagA).CagA is an oncoprotein that is the protagonist of gastric carcinogenesis associated with prolonged *** *** this sense,small regulatory RNAs(sRNAs)are important macromolecules capable of inhibiting and activating gene *** function allows sRNAs to act in adjusting to unstable environmental conditions and in responding to cellular stresses in bacterial *** discoveries have shown that nickelregulated small RNA(NikS)is a post-transcriptional regulator of virulence properties of ***,including the oncoprotein ***,high concentrations of nickel cause the reduction of NikS expression and consequently this increases the levels of *** addition,NikS expression appears to be lower in clinical isolates from patients with gastric cancer when compared to patients *** that in mind,this minireview approaches,in an accessible way,the most important and current aspects about the role of NikS in the control of virulence factors of *** and the potential clinical repercussions of this modulation.

关键词： Helicobacter pylori Small regulatory RNAs Nickel-regulated small RNA Virulence factors Cytotoxin associated antigen A Gastric cancer

Synergistic Antioxidant Activity in Brazilian Propolis Extract Blends: An in Vitro Study

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Food and Nutrition Sciences 2023年第4期14卷 385-400页

作者： José Dilson Francisco da silva Suslin Raatz Thiel Sabrina Somacal Fernanda Cristina Breda Pimpernelli Jonco dos Santos Osmar damian Prestes Renato Zanella Renius de oliveira Mello Department of Food Science and Technology Federal University of Santa Maria (UFSM) Santa Maria Brazil Department of Animal Science Federal University of Santa Maria (UFSM) Santa Maria Brazil Department of Chemistry Federal University of Santa Maria (UFSM) Santa Maria Brazil

A simplex-lattice mixture design with response surface methodology was used to evaluate in vitro synergistic antioxidant activity of red, green, and brown Brazilian propolis extract blends. The in vitro antioxidant capacity of propolis extract blends was measured using the fluorine method of oxygen radical absorption capacity assay (ORAC assay). A synergistic antioxidant interaction was identified between green and brown propolis extracts, and the predictive model accused a binary mixture composed of 59% green and 41% brown propolis extracts with increased antioxidant activity of about 54%. Our findings suggest a possible reduction in the dosages of these natural antioxidants in their various potential applications, including health and food, thereby proving to be a highly promising alternative for the rational use and valorization of propolis.

关键词： Green Propolis Red Propolis Natural Antioxidants Food Additives Mixture Modeling