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COVID-19 Times: Impact on Information Generation and Data Sharing

Advances in Internet of Things 2022年第3期12卷 65-87页

作者： Eden rodríguez-Flores Saúl Gómez-Manzo Jaime Marcial-Quino Roberto Arreguín-Espinosa eduardo rodríguez-bustamante Departamento de Química de Biomacromoléculas Instituto de Química Universidad Nacional Autónoma de México Ciudad de México Mexico Laboratorio de Bioquímica Genética Instituto Nacional de Pediatría Secretaría de Salud Ciudad de México Mexico Investigador Independiente en área de Salud Ciudad de México Mexico Departamento de Bioingeniería Escuela de Ingeniería y Ciencias Tecnológico de Monterrey Campus Ciudad de México Monterrey Mexico

At the beginning of 2020, human activities were interrupted by a new virus, identified as SARS-CoV-2, which causes COVID-19 disease. The scientific area was no exception: for a certain period, researchers around the world were forced to leave their laboratories and work remotely. There was a global necessity for finding alternatives focused on generating knowledge and publishing data, so repositories of scientific information, such as databases, represented strong support. In the specific case of life sciences, different strategies allowed rapid compilation of data and its sharing worldwide. Therefore, in this work, the impact of the SARS-CoV-2 pandemic on the amount of peer-reviewed and published papers during COVID-19 times was analyzed along with the role of databases. Our results pointed out that an increase in the number of papers belonging to different knowledge fields took place, with the medical field being the most significant. On the other hand, the complete genome of the new virus was sequenced, and repositories were created with sufficient data for monitoring, preventing, and controlling its dissemination. This was the case for the generation of vaccines in addition to potential candidates for drugs against COVID-19. However, although in 2021, vaccines allowed us to gradually return to our activities, databases and the generation of other repositories remain a key point for facing new strains and adapting to a new reality. Finally, this paper discusses joint efforts to tackle the obstacles of the pandemic, not only from a medical but also from the point of view regarding the fight against misinformation.

关键词： COVID-19 Laboratories Closure Data Availability Information Repositories Research Papers Infodemics Vaccines Variants and Reopening

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Effect of treatment failure on the Cag A EPIYA motif in Helicobacter pylori strains from Colombian subjects

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World Journal of Gastroenterology 2017年第11期23卷 1980-1989页

作者： Javier Andres bustamante-Rengifo Andres Jenuer Matta Alvaro Jairo Pazos Luis eduardo Bravo Department of Microbiology School of Basic ScienceUniversidad del Valle Registro Poblacional de Cáncer de Cali Department of PathologySchool of MedicineUniversidad del Valle Department of Biology Universidad de Narino

AIM To evaluate effect of treatment failure on cag A and vac A genotypes in Helicobacter pylori(H. pylori) isolates from *** One hundred and seventy-six participants infected with H. pylori from Colombia were treated during 14 d with the triple-standard therapy. Six weeks later, eradication was evaluated by 13C-Urea breath test. Patients with treatment failure were subjected to endoscopy control; biopsies obtained were used for histopathology and culture. DNA from H. pylori isolates was amplified using primers specific for cag A and vac A genes. The phylogenetic relationships among isolates obtained before and after treatment were established by conglomerate analysis based on random amplified polymorphic DNA(RAPD) *** Treatment effectiveness was at 74.6%. Of the par-ticipants with treatment failure, 25 accepted subjected to a second endoscopy. Prevalence of posttreatment infection was 64%(16/25) and 40%(10/25) by histology and culture, respectively. Upon comparing the cag A and vac A genotypes found before and after therapy, multiple cag A genotypes(cag A-positive and cag A-negative) were found before treatment; in contrast, cag A-negative genotypes decreased after treatment. vac A s1m1 genotype was highly prevalent in patients before and after therapy. The 3'cag A region was successfully amplified in 95.5%(21/22) of the isolates obtained before and in 81.8%(18/22) of the isolates obtained after treatment. In the isolates obtained from patients with treatment failure, it was found that 72.7%(16/22) presented alterations in the number of EPIYA motifs, compared to isolates found before *** Unsuccessful treatment limits colonization by lowvirulence strains resulting in partial and selective eradication in mixed infections, and acts on the cag A-positive strains inducing genetic rearrangements in cag A variable region that produces a loss or gain of EPIYA repetitions.

关键词： Helicobacter pylori RAPD-PCR Treatment failure EPIYA motifs 3’ cag A variable region

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Genomic variability of Helicobacter pylori isolates of gastric regions from two Colombian populations

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World Journal of Gastroenterology 2017年第5期23卷 800-809页

作者： Andrés Jenuer Matta Alvaro Jairo Pazos Javier Andrés bustamante-Rengifo Luis eduardo Bravo Registro Poblacional de Cáncer de Cali Department of PathologySchool of MedicineUniversidad del Valle Department of Biology Universidad de Narino

AIMTo compare the genomic variability and the multiple colonization of Helicobacter pylori (H. pylori) in patients with chronic gastritis from two Colombian populations with contrast in the risk of developing gastric cancer (GC): Túquerres-Nariño (High risk) and Tumaco-Nariño (Low risk).METHODSFour hundred and nine patients from both genders with dyspeptic symptoms were studied. Seventy-two patients were included in whom H. pylori was isolated from three anatomic regions of the gastric mucosa, (31/206) of the high risk population of GC (Túquerres) and (41/203) of the low risk population of GC (Tumaco). The isolates were genotyped by PCR-RAPD. Genetic diversity between the isolates was evaluated by conglomerates analysis and multiple correspondence *** proportion of virulent genotypes of H. pylori was 99% in Túquerres and 94% in Tumaco. The coefficient of similarity of Nei-Li showed greater genetic diversity among isolates of Túquerres (0.13) than those of Tumaco (0.07). After adjusting by age, gender and type of gastritis, the multiple colonization was 1.7 times more frequent in Túquerres than in Tumaco (P = 0.05).CONCLUSIONIn Túquerres, high risk of GC there was a greater probability of multiple colonization by H. pylori. From the analysis of the results of the PCR-RAPD, it was found higher genetic variability in the isolates of H. pylori in the population of high risk for the development of GC.

关键词： Helicobacter pylori Pathogenicity islet cag cagA vacA Multiple colonization PCR-RAPD

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Optimal length of triple therapy for H pylori eradication in a population with high prevalence of infection in Chile

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World Journal of Gastroenterology 2007年第21期13卷 2967-2972页

作者： Arnoldo Riquelme Alejandro Soza Cesar Pedreros Andrea bustamante Felipe Valenzuela Francisco Otarola eduardo Abbott Marco Arellano Brenda Medina Alejandro Pattillo Douglas Greig Marco Arrese Antonio Rollan Department of Gastroenterology Pontificia Universidad Católica de Chile Hospital de Urgencia Asistencia Pública SantiagoChile Department of Internal Medicine Pontificia Universidad Católica de Chile

AIM:To compare the efficacy of 7-d versus 14-d triple therapy for the treatment of H pylori infection in Chile,with a prevalence of 73% in general population. METHODS:H pylori-infected patients diagnosed by rapid urease test,with non-ulcer dyspepsia or peptic ulcer disease were randomized to receive omeprazole 20 mg bid,amoxicillin 1 g bid,and clarithromycin 500 mg bid for 7 (OAC7) or 14 (OAC14) d. Primary outcome was eradication rate 6 wk after the treatment. Subgroup analysis was carried out considering the eradication rate among patients with or without peptic ulcer disease and eradication rate among smokers or non-smokers. RESULTS:One hundred and thirty-one patients were randomized to OAC7 (n = 69) or OAC14 (n = 62). The overall eradication rate (intention-to-treat) was 78.3% in OAC7 and 85.5% in OAC14 groups,without a significant difference (P =0.37). No significant difference in the eradication rate was found among the patients with peptic ulcer disease (n = 31) between the OAC7 group (85.7%) and OAC14 group (87.5%). However,smokers had an obviously lower eradication rate compared to non-smokers,particularly in the OAC7 group (57.1% in smokers vs 83.6% in non-smokers; P = 0.06). Adverse effects rate were similar between both groups. CONCLUSION:Short-term efficacy of triple therapy with OAC for 7 d is comparable to 14 d in this high-prevalence population. Longer follow-up,and studies focused to some subgroups of patients (smokers and non-ulcerpatients) are necessary to support widespread use of 7-d instead of 10-14-d triple therapy in a developing country like Chile.

关键词： Helicobacter pylori Antibiotic treatment Omeprazole Amoxicillin Clarithromycin Quasirandomized controlled trial

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In vitro effect of amoxicillin and clarithromycin on the 3' region of cagA gene in Helicobacter pylori isolates

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World Journal of Gastroenterology 2013年第36期19卷 6044-6054页

作者： Javier Andrés bustamante-Rengifo Andrés Januer Matta Alvaro Pazos Luis eduardo Bravo Registro Poblacional de Cáncer de Cali Department of Pathology School of Medicine Universidad del Valle Department of Biology Universidad de Nario

AIM:To evaluate the in vitro effect of amoxicillin and clarithromycin on the cag pathogenicity island(cag PAI).METHODS:One hundred and forty-nine clinical isolates of Helicobacter pylori(***)cultured from gastric biopsies from 206 Colombian patients with dyspeptic symptoms from a high-risk area for gastric cancer were included as study *** susceptibility was determined by the agar dilution *** isolates at baseline and in amoxicillin and clarithromycin serial dilutions were subjected to genotyping(cagA,vacA alleles s and m),Glu-Pro-Ile-Tyr-Ala(EPIYA)polymerase chain reaction and random amplified polymorphic DNA(RAPD).Images of the RAPD amplicons were analyzed by Gel-Pro Analyzer *** analyses was done using SPSS 15.0statistical package,where each of the fingerprint bands were denoted as *** were designed by following Ward’s clustering method and the estimation of distances between each pair of *** isolates was calculated with the squared Euclidean ***:Resistance rates were 4%for amoxicillin and 2.7%for clarithromycin with 2%double *** evidenced a high prevalence of the genotype cagA-positive/vacA *** 3’region of cagA gene was successfully amplified in 92.3%(12/13)of the baseline resistant isolates and in 60%(36/60)of the resistant isolates growing in antibiotic *** observing the distribution of the number of EPIYA repetitions in each dilution with respect to baseline isolates,it was found that in 61.5%(8/13)of the baseline isolates,a change in the number of EPIYA repetitions lowered antibiotic *** gain and loss of EPIYA motifs resulted in a diversity of *** subclones after bacterial adjustment to changing conditions product of antibiotic *** PCR evidenced the close clonal relationship between baseline isolates and isolates growing in antibiotic ***:Antibiotic pressure does not induce loss of the cag pathoge

关键词： Helicobacter pylori Antimicrobial susceptibility cag pathogenicity island cagA 3’region Random amplified polymorphic DNA-polymerase chain reaction

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Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model

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World Journal of Stem Cells 2019年第2期11卷 100-123页

作者： Helio rodrigues da Silva Javier bustamante Mamani Mariana Penteado Nucci Leopoldo Penteado Nucci Andrea Tiemi Kondo Daianne Maciely Carvalho Fantacini Lucas eduardo Botelho de Souza Virginia Picanço-Castro Dimas Tadeu Covas José Mauro Kutner Fernando Anselmo de Oliveira Nelson Hamerschlak Lionel Fernel Gamarra Hospital Israelita Albert Einstein LIM44 Hospital das Clinicas HCFMUSP Faculdade de MedicinaUniversidade de São Paulo Centro Universitário do Planalto Central Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo

BACKGROUND Mesenchymal stem cells(MSCs) have been widely tested for their therapeutic efficacy in the ischemic brain and have been shown to provide several benefits. A major obstacle to the clinical translation of these therapies has been the inability to noninvasively monitor the best route, cell doses, and collateral effects while ensuring the survival and effective biological functioning of the transplanted stem cells. Technological advances in multimodal imaging have allowed in vivo monitoring of the biodistribution and viability of transplanted stem cells due to a combination of imaging technologies associated with multimodal nanoparticles(MNPs) using new labels and covers to achieve low toxicity and longtime residence in *** To evaluate the sensitivity of triple-modal imaging of stem cells labeled with MNPs and applied in a stroke *** After the isolation and immunophenotypic characterization of human bonemarrow MSCs(hBM-MSCs), our team carried out lentiviral transduction of these cells for the evaluation of bioluminescent images(BLIs) in vitro and in vivo. In addition, MNPs that were previously characterized(regarding hydrodynamic size, zeta potential, and optical properties), and were used to label these cells,analyze cell viability via the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and BLI analysis, and quantify the internalization process and iron load in different concentrations of MNPs via magnetic resonance imaging(MRI),near-infrared fluorescence(NIRF), and inductively coupled plasma-mass spectrometry(ICP-MS). In in vivo analyses, the same labeled cells were implanted in a sham group and a stroke group at different times and under different MNP concentrations(after 4 h or 6 d of cell implantation) to evaluate the sensitivity of triple-modal *** hBM-MSC collection and isolation after immunophenotypic characterization were demonstrated to be adequate in hBM samples. After transduction of these cells with

关键词： Multimodal nanoparticles Human bone marrow mesenchymal stem cells Near-infrared fluorescence image Magnetic resonance image Bioluminescence Stroke

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墨西哥城N.A.A.F.I指南

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青年视觉 2018年第3期 236-249页

作者： eduardo Dali Yue Zhu Emilio Espejel 不详

N.A.A.F.I的诞生可以追溯至2010年,源自于其创始人发起的一系列深夜浩室派对,这包括瓦哈卡当地DJ Tomas Dav6和Alberto bustamante。他们主要是为朋友们播放自己的曲目,然而很快就史无前例地统治了墨西哥城的地下俱乐部现场。这个群体... 详细信息

N.A.A.F.I的诞生可以追溯至2010年,源自于其创始人发起的一系列深夜浩室派对,这包括瓦哈卡当地DJ Tomas Dav6和Alberto bustamante。他们主要是为朋友们播放自己的曲目,然而很快就史无前例地统治了墨西哥城的地下俱乐部现场。这个群体已经扩充到有超过16名成员,包括Lauro Rob(Lao),les Ilmaabs,Zakmatic,Wasted Fates(Octavio Kh),Zutzut和Lechuga Zafiro等来自全世界的常客,每个人都用他们独特的声音探索拉美身份,同时混合部落风格(低预算,快节奏的电子流行乐),雷击顿和西方电子流派;从伴随着器乐放克的90年代重低音欧洲流行到俗气的主流流行乐人声。

关键词：重低音墨西哥城 A.A

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