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Protection status as determinant of carbon stock drivers in Cerrado sensu stricto

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Journal of Plant Ecology 2020年第3期13卷 361-368页

作者： Kelly Marianne Guimarães Pereira Natielle gomes Cordeiro Marcela de Castro Nunes Santos Terra Marcela Venelli Pyles Christian Dias Cabacinha JoséMárcio de Mello eduardo van den Berg Department of Biology Federal University of LavrasCampus UniversitárioCEP 37200-000LavrasMinas Gerais stateBrazil Department of Forest Sciences Federal University of LavrasCampus UniversitárioCEP 37200-000LavrasMinas Gerais stateBrazil Department of Forest Sciences Institute of Agrarian SciencesFederal University of Minas GeraisCampus Regional de Montes ClarosCEP 39.404-547Montes ClarosMinas Gerais stateBrazil

天然植被在全球碳循环和碳储存中扮演着重要角色。巴西大草原塞拉多保护区(Cerrado)因自身固有特性被认为是一个碳汇。本研究的目的是评估具有不同保护状况的三个地区,控制区(法定保护区)、保护区(PA)和非保护区(Non-PA)地上生物量与生... 详细信息

天然植被在全球碳循环和碳储存中扮演着重要角色。巴西大草原塞拉多保护区(Cerrado)因自身固有特性被认为是一个碳汇。本研究的目的是评估具有不同保护状况的三个地区,控制区(法定保护区)、保护区(PA)和非保护区(Non-PA)地上生物量与生物多样性关系的变化。这三个被研究的地区都位于巴西米纳斯吉拉斯州(Minas Gerais)北部。根据森林清查资料,该研究对地上碳储量进行了估算,并测量了每个地区生物多样性指标的三个维度:功能性状优势度、分类学多样性和功能多样性。对物种的以下功能性状进行了评价:木材密度、最大直径和种子大小。通过建立广义线性模型,评估了碳储量、群落加权平均值、物种丰富度和多样性以及功能多样性指数在不同地区间的差异。研究结果表明,未受保护的地区碳储量、物种丰富度、物种多样性、功能丰富度和功能分散度均较低,而保护区和非保护区群落加权平均值最大直径和种子大小均低于法定保护区。广义线性模型结果表明,碳储量与物种和功能丰富度指数在同一地区内和不同地区间存在相关性,因此,物种丰富度可以作为功能丰富度和碳储量的替代指标。物种丰富度和群落加权平均值最大直径对碳储量有正向影响,功能分散度对碳储量有负向影响。功能丰富度、物种多样性和群落加权平均值种子大小出现在最佳模型中,但对碳储量没有显著的直接影响。因此,我们的结论是,在缺乏保护的巴西塞拉多地区会降低物种丰富度和碳储量。

关键词：功能性状生物多样性生物量储存巴西大草原人类影响

Non-wetting Additive as a Solution to Minimize the Corrosion of Refractory Castables Against Molten Aluminum

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China's Refractories 2016年第1期25卷 20-25页

作者： Fernando VERNILLI Adriel Claro de FARIA Mateus gomes VERNILLI Augusto Celso AMOEDO eduardo Jose Togni CARDILLO Taner Augusto MAIA Matheus MARTINI Engineering School of Lorena University of Sao Paulo Lorena 12600-970 Brazil Togni S/A Materiais Refratarios Pocos de Caldas 37704-356 Brazil

The ternary compound barium aluminosilicate BaAl2Si2O8, known as celsian, a non-wetting additive that increases the corrosion resistance of refractor） materials against molten aluminum, was incorporated into the matrix of a high-alumina low cement castable after its synthesis using barite and a low cost source of alumina and silica. This paper presents the influence of this phase on the wetting angle formed between liquid aluminum alloy and solid refractory castable surface.

关键词： refractories con'osion non-wetting additive aluminmn alloy

Addition of respiratory exercises to conventional rehabilitation for children and adolescents with cerebral palsy:a systematic review and meta-analysis

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World Journal of Pediatrics 2023年第4期19卷 340-355页

作者： Thálita Raysa de Lima Crispim Mansueto gomes Neto Tafnes Rayane Lima Crispim Renivaldo Batista Dias Maria Déborah Monteiro de Albuquerque Micheli Bernardone Saquetto Paulo André Freire Magalhães Research Group of Neonatal and Pediatric Physical Therapy Baby GrUPEPetrolinaPernambucoBrazil Postgraduate Program in Rehabilitation and Functional Performance PPGRDFUniversidade de PernambucoRodovia BR 203Km 2 S/N.Vila EduardoPetrolinaPernambuco56328-900Brazil Research Group of Physiotherapy Department of Physical TherapyUniversidade Federal da BahiaSalvadorBrazil Posgraduate Program in Medicine and Health SalvadorBahiaBrazil

Background Respiratory dysfunctions are an important cause of morbidity and death in cerebral palsy(CP)*** exercises in addition to conventional rehabilitation have been suggested to improve respiratory status in CP *** objective of this systematic review and meta-analysis was to verify the effects of the addition of respiratory exercises to conventional rehabilitation on pulmonary function,functional capacity,respiratory muscle strength,gross motor function and quality of life in children and adolescents with *** We searched for randomized controlled clinical trials in PubMed/Medline,Lilacs,SciELO,EMBASE and Physi-otheraphy Evidence(PEDro)from their inception until July 2022 without language *** that included respiratory exercises(breathing exercise program;feedback respiratory training;incentive spirometer exercise;inspiratory muscle training;and combination of respiratory exercises+incentive spirometer exercise)in combination with conventional rehabilitation for children and adolescents with CP were evaluated by two independent *** mean difference(MD)and 95%confidence interval(CI)were estimated by random effect *** Ten studies met the eligibility criteria,including 324 children aged from 6 to 16 *** meta-analysis showed an improvement in inspiratory muscle strength of 22.96 cmH2O(18.63-27.27,n=55)and pulmonary function of 0.60(0.38-0.82,n=98)for forced vital capacity(L);0.22(0.06-0.39,n=98)for forced expiratory volume at 1 second(L);and 0.50(0.05-0.04,n=98)for peak expiratory flow(L/min).Functional skills in daily living activities improved in the intervention ***'assistance of daily living activities,functional capacity,gross motor function and expiratory muscle strength showed a nonsignificant *** well-being and acceptance and functioning domains improved in only one *** Emerging data show significant enhancements in inspiratory muscle stren

关键词： Breathing exercises Cerebral palsy Inspiratory muscle training Pediatrics Rehabilitation Respiratory exercise

Hepatobiliary phases in magnetic resonance imaging using liverspecific contrast for focal lesions in clinical practice

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World Journal of Hepatology 2022年第7期14卷 1459-1469页

作者： Daniel Alvarenga Fernandes eduardo Andreazza Dal Lago Felipe Aguera Oliver Bruna Melo Coelho Loureiro Daniel Lahan Martins Thiago José Penachim Ricardo Hoelz de Oliveira Barros José de ArimatéiaBatista Araújo Filho Larissa Bastos Eloy da Costa Áurea Maria Oliveira da Silva Elaine Cristina de Ataíde Ilka de Fátima Santana Ferreira Boin Nelson Marcio gomes Caserta Department of Radiology School of Medical SciencesUniversity of Campinas-UNICAMPCampinas 13083-888São PauloBrazi Department of Radiology Medical SchoolSão Paulo State University-UNESPBotucatu 18618-970São PauloBrazil Instituto de Radiologia Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo-InRad/HC-FMUSPSão Paulo 05403-010SPBrazil Department of Radiology Sírio-Libanês HospitalSão Paulo 05652-900SPBrazil Department of Pathology School of Medical SciencesUniversity of Campinas-UNICAMPCampinas 13083-888São PauloBrazil Liver Transplant Unit Department of SurgerySchool of Medical SciencesUniversity of Campinas-UNICAMPCampinas 13083-888São PauloBrazil

BACKGROUND Challenging lesions,difficult to diagnose through non-invasive methods,constitute an important emotional burden for each patient regarding a still uncertain diagnosis(malignant x benign).In addition,from a therapeutic and prognostic point of view,delay in a definitive diagnosis can lead to worse *** of the main innovative trends currently is the use of molecular and functional methods to *** liver-specific contrast agents havebeen developed and studied in recent years to improve the performance of liver magnetic resonance imaging(MRI).More recently,one of the contrast agents introduced in clinical practice is gadoxetic acid(gadoxetate disodium).AIM To demonstrate the value of the hepatobiliary phases using gadoxetic acid in MRI for the characterization of focal liver lesions(FLL)in clinical *** Overall,302 Lesions were studied in 136 patients who underwent MRI exams using gadoxetic acid for the assessment of *** radiologists independently reviewed the MRI exams using four stages,and categorized them on a 6-point scale,from 0(lesion not detected)to 5(definitely malignant).The stages were:stage 1-images without contrast,stage 2-addition of dynamic phases after contrast(analogous to usual extracellular contrasts),stage 3-addition of hepatobiliary phase after 10 min(HBP 10’),stage 4-hepatobiliary phase after 20 min(HBP 20’)in addition to stage *** The interobserver agreement was high(weighted Kappa coefficient:0.81-1)at all stages in the characterization of benign and malignant *** diagnostic weighted accuracy(Az)was 0.80 in stage 1 and was increased to 0.90 in stage *** of the hepatobiliary phase increased Az to 0.98 in stage 3,which was also 0.98 in stage *** The hepatobiliary sequences improve diagnostic *** growing potential in the era of precision medicine,the improvement and dissemination of the method among medical specialties can bring benefits in the management of patients w

关键词： Liver Liver neoplasms Liver transplantation Medical oncology Diagnostic imaging Magnetic resonance imaging

Superoxide Anion Release by Human Blood Phagocytes Can Increase the Microbicidal Activity Induced by a New Microemulsioned System Containing Cotton Oil

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Advances in Biological Chemistry 2022年第6期12卷 207-227页

作者： Luana Cristina da Silva Ramos André Henrique Furtado Torres Letícia Damas Leão Dalcin Katleyn Polizeli Galvão Silva Luana Gabriela Silva de Freitas Mahmi Fujimori Danny Laura gomes Fagundes eduardo Luzia França Adenilda Cristina Honorio França Universidade Federal de Mato Grosso. Barra do Garç as Brazil

The study aimed to develop and characterize a microemulsified system based on cotton oil and verify its effect on superoxide release anion and microbicidal activity by human peripheral blood cells. Microemulsions were formulated using distilled water, degummed cotton oil, Span 80 (SP), Tween 80 (TW), and 1-butanol (BT). The pseudo-ternary diagram delimited ME regions, and the points were pre-selected. The physical-chemical and rheological characterization of the microemulsions was carried out. The ME activity on the interactions between leukocytes and bacteria was analyzed by superoxide release, phagocytosis, and microbicidal activity. The developed formulation was classified as Oil/Water, with an average pH of 5.76, and the viscosity showed resistance to temperature changes. The rheological model of the Power Law classified the microemulsion as a non-Newtonian fluid with pseudoplastic characteristics. The cell viability of cotton oil microemulsion was greater than 90%. There was an increase in the superoxide release by MN phagocytes when treated with cotton oil microemulsion. The cotton oil microemulsion increased phagocytosis and microbicidal activity. The present study suggests that cotton oil is an alternative biomaterial for therapeutic applications, especially in treating infections.

关键词： Cotton Oil Microemulsion Phagocytosis Infection Human Phagocytes

Balloon-assisted enteroscopy for suspected Meckel's diverticulum and indefinite diagnostic imaging workup

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World Journal of Gastrointestinal Endoscopy 2016年第18期8卷 679-683页

作者： Guilherme Francisco gomes eduardo Aimore Bonin Rafael William Noda Leandro Totti Cavazzola Thiago Ferreira Bartholomei Endoscopy Unit Nossa Senhora das Gracas Hospital Curitiba 81830 Brazil Universidade Federal do Rio Grande do Sul Porto Alegre 91740 Brazil

Meckel's diverticulum(MD) is estimated to affect 1%-2% of the general population, and it represents a clinically silent finding of a congenital anomaly in up to 85% of the cases. In adults, MD may cause symptoms, such as overt occult lower gastrointestinal bleeding. The diagnostic imaging workup includes computed tomography scan, magnetic resonance imaging enterography, technetium 99 m scintigraphy(99mTc) using either labeled red blood cells or pertechnetate(known as the Meckel's scan) and angiography. The preoperative detection rate of MD in adults is low, and many patients ultimately undergo exploratory laparoscopy. More recently, however, endoscopic identification of MD has been possible with the use of balloon-assisted enteroscopy via direct luminal access, which also provides visualization of the diverticular ostium. The aim of this study was to review the diagnosis by double-balloon enteroscopy of 4 adults with symptomatic MD but who had negative diagnostic imaging workups. These cases indicate that balloonassisted enteroscopy is a valuable diagnostic method and should be considered in adult patients who have suspected MD and indefinite findings on diagnostic imaging workup, including negative Meckel's scan.

关键词： Double-balloon enteroscopy Meckel’s diverticulum Diagnosis

Assessment of LLLT systemic effects on thyroid hormones function after dental titanium implant installation:An experimental rabbit model

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Natural Science 2013年第8期5卷 933-940页

作者： Luciano Mayer Fernando Vacilotto gomes Carlos eduardo Baraldi Joao Batista Blessmann Weber Marília Gerhardt de Oliveira Department of Oral and Maxillofacial Surgery School of DentistryPontifícia Universidade Católica do Rio Grande do SulPorto AlegreBrazil Oral and Maxillofacial Surgery and Traumatology—Department of Surgery Universidade Federal do Rio Grande do SulSchool of DentistryRio Grande do SulBrazil Oral and Maxillofacial Surgery Service Hospital Cristo Redentor—Grupo Hospitalar ConceicaoPorto AlegreBrazil

This study aimed to assess the systemic effect of LLLT on thyroid gland functioning and consequently on calcium regulation through Triiodothyronine (T3) and Thyroxine (T4) measurements in rabbits’ serum. A total of thirty two New Zealand male rabbits were randomly distributed in four groups with eight animals each: control group C (nonirradiated animals), group EI (5 J/cm2 per session), group EII (10 J/cm2 per session) and group EIII (20 J/cm2 per session). All animals underwent lower left incisor extraction followed by immediate insertion of an osseintegrated implant, providing an equality of initial clinical condition between the groups. The experimental groups were irradiated with aluminium gallium arsenide diode laser (GaAlAs, λ = 830 nm, 50 mW, CW), during 13 days at each 48 hours, totalizing 7 sessions. Laboratorial T3 and T4 measurements were done in four distinct moments (before surgical procedure, immediately after surgical procedure, after the first LLLT session and after the last LLLT session) in all animals. The results obtained showed statistically significant differences in Triiodothyronine values between the groups throughout the experiment. It was concluded that the LLLT, in the protocol of irradiation used in this study, promoted a significantly alteration on rabbits’ serum hormonal levels.

关键词： Thyroid Gland Laser Therapy Low Level Thyroid Hormones Dental Implant

Biocatalytic production of chitosan polymers from shrimp shells,using a recombinant enzyme produced by Pichia pastoris

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American Journal of Molecular Biology 2012年第4期2卷 341-350页

作者： eduardo Mere Del Aguila Laidson Paes gomes Cristina Tristao Andrade Joab Trajano Silva Vania Margaret Flosi Paschoalin Universidade Federal do Rio de Janeiro Instituto de QuímicaRio de JaneiroBrazil Universidade Federal do Rio de Janeiro Instituto de Macromoléculas Professora Eloisa ManoRio de JaneiroRJBrazil

Chitosan has a unique chemical structure with high charge density, reactive hydroxyl and amino groups, and extensive hydrogen bonding. Chitin deacetylase (EC 3.***.1.41) catalyzes the hydrolysis of the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin, converting it to chitosan and releasing acetate. The entire ORF of the CDA2 gene encoding one of the two isoforms of chitin deacetylase from Saccharomyces cerevisiae was cloned in Pichia pastoris. The Tg (Cda2-6xHis)p was expressed at high levels as a soluble intracellular protein after induction of the recombinant yeast culture with methanol, and purified using nickel-nitrilotriacetic acid chelate affinity chromatography, resulting in a protein preparation with a purity of >98% and an overall yield of 79%. Chitin deacetylase activity was measured by a colorimetric method based on the O-phthalaldehyde reagent, which detects primary amines remaining in chitinous substrate after acetate release. The recombinant enzyme could deacetylate chitin, chitobiose, chitotriose and chitotetraose, with an optimum temperature of 50°C and pH 8.0, determined using oligochitosaccharides as the substrates. The recombinant protein was also able to deacetylate its solid natural substrate, shrimp chitin, to a limited extent, producing chitosan with a degree of acetylation (DA) of 89% as determined by Fourier transform infrared spectroscopy. The degree of deacetylation was increased by pre-hydrolysis of crystalline shrimp chitin by chitinases, which increased the deacetylation ratio triggered by chitin deacetylase, producing chito-oligosaccharides with a degree of acetylation of 33%. The results described here open the possibility to use the rCda2p, combined with chitinases, for biocatalytic conversion of chitin to chitosan with controlled degrees of deacetylation. We show herein that the crystalline chitin form can be cleanly produced in virtually quantitative yield if a combined and sequential enzyme treatment is performed.

关键词： Chitin Deacetylase Chitosan Chitin Pichia Pastoris Deacetylation Degree

Conservative Surgery for Subungual Melanoma In Situ Using Matriderm^(■)

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Journal of Cancer Therapy 2017年第10期8卷 861-866页

作者： Mariane Campagnari Helio Alberto Carneiro Andrea Schiavinato Jafelicci Heidy Carmecide da Silva Reis Elimar Elias gomes eduardo Bertolli Joao Pedreira Duprat Neto Skin Cancer Department AC Camargo Cancer CenterSao PauloBrazil Surgical Oncology Residence AC Camargo Cancer CenterSao PauloBrazil

Background: Standard treatment for subungual melanoma is wide local excision including digit amputation in order to obtain safety margins. Level of amputation has been discussed in order to achieve appropriate oncological results along with preservation of limb function wherein local excision of subungual melanoma in situ without amputation is an option. Methods: We report two cases of subungual melanoma in situ treated by using a conservative approach. Both cases were treated with local excision with the resected tumor defect covered with an artificial dermis, Matriderm?. One case was of a 65-year-old male with a left thumb subungual melanoma in situ and another of a 41-year-old female with the same type of melanoma in the left hallux. Full-thickness skin graft was used for reconstruction after 7 weeks from the first surgery in the latter, and in the former case re-epithelization occurred within 90 days after surgery. To date, no clinical signs of recurrence have been found, and finger/toe functions were completely preserved with a minimized scar and little graft bed sensitivity. Follow-up time is two years and ten months for the left thumb case, and one year and six months for the left hallux subungual melanoma in situ. Conclusion: We present two cases of subungual melanoma in situ treated with digit sparing surgery including excision of the periosteum followed by the use of an artificial dermal template to guarantee coverage of the tumor bed defect, providing good functional and cosmetic outcome.

关键词： Melanoma Amputation Artificial Skin