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Interactions between maternal parity and feed additives drive the composition of pig gut microbiomes in the post‑weaning period

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1241-1257页

作者： Kayla Law eduardo rosa Medina Garcia Chad Hastad Deborah Murray Pedro E.Urriola Andres Gomez Department of Animal Science University of Minnesota1364 Eckles AvenueSaint PaulMN 55108USA New Fashion Pork 164 Industrial ParkwayJacksonMN 56143USA

Background Nursery pigs undergo stressors in the post-weaning period that result in production and welfare *** challenges disproportionately impact the offspring of primiparous sows compared to those of mul-tiparous *** is known regarding potential interactions between parity and feed additives in the post-weaning period and their effects on nursery pig ***,the objective of this study was to investigate the effects of maternal parity on sow and offspring microbiomes and the influence of sow parity on pig fecal microbi-ome and performance in response to a prebiotic *** weaning,piglets were allotted into three treat-ment groups:a standard nursery diet including pharmacological doses of Zn and Cu(Con),a group fed a commercial prebiotic only(Preb)based on an Aspergillus oryzae fermentation extract,and a group fed the same prebiotic plus Zn and Cu(Preb+ZnCu).Results Although there were no differences in vaginal microbiome composition between primiparous and mul-tiparous sows,fecal microbiome composition was different(R^(2)=0.02,P=0.03).The fecal microbiomes of primiparous offspring displayed significantly higher bacterial diversity compared to multiparous offspring at d 0 and d 21 post-weaning(P<0.01),with differences in community composition observed at d 21(R^(2)=0.03,P=0.04).When analyzing the effects of maternal parity within each treatment,only the Preb diet triggered significant microbiome distinc-tions between primiparous and multiparous offspring(d 21:R^(2)=0.13,P=0.01;d 42:R^(2)=0.19,P=0.001).Composi-tional differences in pig fecal microbiomes between treatments were observed only at d 21(R^(2)=0.12,P=0.001).Pigs in the Con group gained significantly more weight throughout the nursery period when compared to those in the Preb+ZnCu *** Nursery pig gut microbiome composition was influenced by supplementation with an Aspergillus oryzae fermentation extract,with varying effects on performance

关键词： Aspergillus prebiotic Copper Feed additives Maternal Nursery pig microbiome Parity Swine microbiome Trace minerals Zinc

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Extraction of Phenolic Compounds from Olive Leaf Extracts and Their Effect on Proliferation of Human Carcinoma Cell Lines

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Agricultural Sciences 2019年第10期10卷 1271-1285页

作者： Diana Martinho Amin Karmali eduardo rosa Chemical Engineering and Biotechnology Research Center Department of Chemical Engineering of Instituto Superior de Engenharia de Lisboa Lisboa Portugal Centre for the Research and Technology for Agro-Environmental and Biological Sciences Universidade de Trás-os-Montes e Alto Douro Vila Real Portugal.

The aim of this work was to evaluate the effect of different olive leaf extracts (OLE) from different leaf growing stages on human carcinoma cell lines. OLE were tested in human carcinoma cell lines in vitro and cells were plated in 96-microtiter culture plates for each OLE concentration. Fresh (F) and freeze-dried (FD) leaves exhibited phenolic compounds in the range of 2.09 ± 0.10 to 8.44 ± 0.64 and 7.72 ± 0.56 to 24.65 ± 1.9 mg gallic acid equivalents/g leaves, respectively. OLE from several Portuguese olive tree cultivars were found to inhibit the growth of human carcinoma cell lines in a range of 2.09 - 8.44 μg phenolic compound/well (209 - 844 μg/ml) and 0.07 - 2.40 μg phenolic compounds/well (7 - 240 μg/ml) for fresh and freeze-dried leaves, respectively. Young (Y) leaves have revealed the highest cell growth inhibition ranging from about 95% for Cobran?osa, followed by 90% for Cobran?osa, 90% for Arbequina and 75% for Arbequina for cell lines A549, HeLa, A431 and OE21, respectively. The lowest cell growth inhibition (35%) was observed for Galega (Y) leaf extract on cell line A549. However, FD samples exhibited a distinctive pattern since cell growth inhibition was highest at highest extract dilution tested, for A431 (Galega Y) followed by A549 (Cobran?osa Y) with cell inhibition of 75% and 70%, respectively. The data presented in this work strongly suggest that OLEs inhibit the growth of human carcinoma cell lines.

关键词： Olive Leaf Extracts Anti-Proliferation of Human Carcinoma Cell Lines Freeze-Dried Leaves Phenolic Compounds Younger and Older Olive Leaves Portuguese Olive Cultivars

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Tailings Dam Mining, Theoretical Considerations, and Circular Economy: A Review

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Journal of Geoscience and Environment Protection 2024年第9期12卷 77-92页

作者： eduardo da rosa Aquino Vidal Félix Navarro Torres Irvyn Laurence Paniz Instituto Tecnolgico Vale - Minerao Ouro Preto Brazil

Mining in tailings dams has emerged as a strategic alternative for mining companies for both economic and environmental reasons. Owing to technological limitations in recent decades, many of these dams have high metal contents, emphasizing the need to evaluate the quality of these residues, especially considering the technological advancements in current concentration plants. An economic viability analysis associated with reusing these materials is crucial. From an environmental point of view, improving mining techniques for dams by considering both safety and feasibility is an advantageous option in decommissioning processes and alignment in the circular economy. In this context, representing these tailings in terms of grade quality and granulometry, as well as the associated contaminants, is essential. Geostatistical estimation and simulation methods are valuable tools for modeling tailings bodies, but they require a reliable sampling campaign to ensure acceptably low errors. From an operational perspective, tailings recovery can be conducted via dry methods, such as mechanical excavation, or hydraulic methods, such as dredging or hydraulic blasting. Dredging is a commonly used method, and cutter suction dredgers, which require pumping to transport fragmented material, are the most commonly used tools. In this paper, some practical applications of geostatistical methods for resource quantification in tailings dams will be discussed. Additionally, the main mining methods for tailings recovery in dams will be presented. Emphasis will be given to the dredging method, along with the key analysis parameters for sizing dredgers, pumps, and pipelines.

关键词： Mining in Tailings Dams Geostatistical Methods Grade Quality Dredging

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In vivo tracking of tau pathology using positron emission tomography (PET) molecular imaging in small animals

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Translational Neurodegeneration 2014年第1期3卷 34-39页

作者： eduardo Rigon Zimmer Antoine Leuzy Venkat Bhat Serge Gauthier Pedro rosa-Neto Translational Neuroimaging Laboratory(TNL) McGill Center for Studies in AgingDouglas Mental Health University InstituteMcGill University6875 La Salle Blv-FBC room 3149MontrealQCH4H 1R3Canada Montreal Neurological Institute(MNI) MontrealCanada Department of Biochemistry Federal University of Rio Grande do Sul(UFRGS)Porto AlegreBrazil Department of Psychiatry McGill UniversityMontrealBrazil

Hyperphosphorylation of the tau protein leading to the formation of neurofibrillary tangles(NFTs)is a common feature in a wide range of neurodegenerative diseases known as tauopathies,which include Alzheimer’s disease(AD)and the frontotemporal dementias(FTDs).Although heavily investigated,the mechanisms underlying the pathogenesis and progression of tauopathies have yet to be fully *** this context,several rodent models have been developed that successfully recapitulate the behavioral and neurochemical features of tau pathology,aiming to achieve a better understanding of the link between tau and *** date,behavioral and biochemical parameters assessed using these models have been conducted using a combination of memory tasks and invasive methods such as cerebrospinal fluid(CSF)sampling or post-mortem ***,several novel positron emission tomography(PET)radiopharmaceuticals targeting tau tangles have been developed,allowing for non-invasive in vivo quantification of tau *** with tau transgenic models and micro-PET,these tracers hold the promise of advancing the development of theoretical models and advancing our understanding of the natural history of AD and non-AD *** this review,we briefly describe some of the most important insights for understanding the biological basis of tau pathology,and shed light on the opportunity for improved modeling of tau pathology using a combination of tau-radiopharmaceuticals and animal models.

关键词： Positron emission tomography Tau molecular agents Tau rodent models Tauopathies

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Laser-Treated Surfaces for VADs:From Inert Titanium to Potential Biofunctional Materials

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Biomedical Engineering Frontiers 2022年第1期3卷 399-406页

作者： eduardo Bock Wilhelm Pfleging Dayane Tada Erenilda Macedo Nathalia Premazzi rosa Sá Juliana Solheid Heino Besser Aron Andrade Laboratory of Bioengineering and Biomaterials Federal Institute of Technology in Sao Paulo(IFSP)Sao PauloBrazil Center of Engineering in Circulatory Assistance Institute Dante Pazzanese of Cardiology(IDPC)Sao PauloBrazil Institute for Applied Materials-Applied Materials Physics Karlsruhe Institute of Technology(KIT)KarlsruheGermany Federal University of Sao Paulo(UNIFESP) Sao Jose dos CamposBrazil National Institute for Space Research(INPE) Sao Jose dos CamposBrazil

***-treated surfaces for ventricular assist *** *** work has scientific impact since it proposes a biofunctional surface created with laser processing in bioinert *** diseases are the world’s leading cause of *** especially debilitating heart disease is congestive heart *** the possible therapies,heart transplantation and mechanical circulatory assistance are the main treatments for its severe form at a more advanced *** development of biomaterials for ventricular assist devices is still being carried *** polished titanium is currently employed in several devices,its performance could be improved by enhancing the bioactivity of its *** to improve the titanium without using coatings that can be detached,this work presents the formation of laser-induced periodic surface structures with a topology suitable for cell adhesion and neointimal tissue *** surface was modified by femtosecond laser ablation and cell adhesion was evaluated in vitro by using fibroblast *** results indicate the formation of the desired topology,since the cells showed the appropriate adhesion compared to the control *** electron microscopy showed several positive characteristics in the cells shape and their surface *** in vitro results obtained with different topologies point that the proposed LIPSS would provide enhanced cell adhesion and *** laser processes studied can create new interactions in biomaterials already known and improve the performance of biomaterials for use in ventricular assist devices.

关键词： titanium biomaterials Titanium

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Liver transplantation is beneficial regardless of cirrhosis stage or acute-on-chronic liver failure grade:A single-center experience

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World Journal of Gastroenterology 2022年第40期28卷 5881-5892页

作者： eduardo Cervantes-Alvarez Mario Vilatoba Nathaly Limon-de la rosa Osvely Mendez-Guerrero David Kershenobich Aldo Torre Nalu Navarro-Alvarez PECEM Facultad de MedicinaUniversidad Nacional Autónoma de MéxicoMexico City 14080Mexico Department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico City 14080Mexico Department of Trasplant Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico City 14080Mexico Department of Molecular Biology Universidad Panamericana School of MedicineMexico City 03920Mexico Department of Surgery University of Colorado Anschutz Medical CampusDenverCO 80045United States

BACKGROUND Liver transplantation for the most critically ill remains controversial;however,it is currently the only curative treatment *** To assess immediate posttransplant outcomes and compare the short(1 year)and long-term(6 years)posttransplant survival among cirrhotic patients stratified by disease *** We included cirrhotic patients undergoing liver transplantation between 2015 and 2019 and categorized them into compensated cirrhosis(CC),decompensated cirrhosis(DC),and acute-on-chronic liver failure(ACLF).ACLF was further divided into severity *** primary outcomes of interest were total days of intensive care unit(ICU)and hospital stay,development of complications and posttransplant survival at 1 and 6 *** 235 patients underwent liver transplantation(CC=11,DC=129 and ACLF=95).Patients with ACLF had a significantly longer hospital stay[8.0(6.0-13.0)vs CC,6.0(3.0-7.0),and DC 7.0(4.5-10.0);P=0.01]and developed more infection-related complications[47(49.5%),vs CC,1(9.1%)and DC,38(29.5%);P<0.01].Posttransplant survival at 1-and 6-years was similar among groups(P=0.60 and P=0.90,respectively).ACLF patients stratified according to ACLF grade[ACLF-1 n=40(42.1%),ACLF-2 n=33(34.7%)and ACLF-3 n=22(23.2%)],had similar ICU and hospital stay length(P=0.68,P=0.54),as well as comparable frequencies of overall and infectious posttransplant complications(P=0.58,P=0.80).There was no survival difference between ACLF grades at 1 year and 6 years(P=0.40 and P=0.15).CONCLUSION Patients may benefit from liver transplantation regardless of the cirrhosis *** patients have a longer hospital stay and frequency of infectious complications;however,excellent,and comparable 1 and 6-year survival rates support their enlisting and transplantation including those with ACLF-3.

关键词： Liver transplantation Acute-on-chronic liver failure Prognosis Survival analysis Critical care

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A Review on Genetically Modified Plants Designed to Phytoremediate Polluted Soils： Biochemical Responses and International Regulation

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Pedosphere 2018年第5期28卷 697-712页

作者： Edgar VAZQUEZ-NUNEZ Julian M. PENA-CASTRO Fabian FERNANDEZ-LUQUENO eduardo CEJUDO Maria G. de la rosa-ALVAREZ Maria C. GARCIA-CASTANED Departamento de Ingenierias Quimica Electronica y Biomedica Division de Ciencias e Ingenierlas Universidad de Guanajuato (UG) Campus Leon Loma del Bosque 103 Leon Gto. 37150 (Mexico) Laboratorio de Biotecnologla Vegetal Instituto de Biotecnologia Universidad del Papaloapan Tuxtepec Oaxaca 68333 (Mexico) programa en Sustentabilidad de los Recursos Naturales y Eneryla Cinvestav Saltillo Industria Metalurgica 1062 Parque Industrial Saltillo-Ramos-Arizpe Ramos Arizpe Coahuila 25900 (Mexico) CONACYT Unidad de Ciencias del Agua (UCIA) Centro de Investigacion Cientifica de Yucatan A.C. Cancdn Quintana Roo 77500 (Mexico) Center for the Environmental Implications of Nanotechnology University of California at Los Angeles- University of Texas at El Paso 500 W University Ave El Paso 79968 (USA) CONACYT Universidad de Guanajuato Loma del Bosque 103 Leon Guanajuato 37150 (Mexico)

In recent years,there has been an increasing interest in finding sustainable strategies for the efficient removal of contaminants from *** objective of this review is to examine the biochemical principles of specific genetic modifications in plants,their applications in the field for specific contaminants as phytotechnologies,and their international *** addition,the review presents some biological aspects of rhizosphere-related phenomena,the interactions of organic and inorganic pollutants with plants,and the performance of the phytotechnologies across the *** the last few decades,at least eight genera of genetically modified plants(GMPs)have been tested and used for soil remediation with outstanding ***,Nicotiana,and Oryza are the plant genera most widely *** plant genes such as metal transporters,chelators,metallothioneins,phytochelatins,and oxygenases have been transferred to plants to improve the elimination of contaminants in *** discuss some important aspects of gene manipulation and its application for removal of diverse contaminants.A key challenge faced by phytotechnologies is the final disposal of the generated biomass,from a safety *** argue that the commercial success of phytotechnologies depends on the generation of valuable biomass on contaminated land and its use for bioenergy *** use of such technologies would promote a broader understanding of the importance of plants,especially GMPs,in the environment and their contribution to environmental sustainability.

关键词： bioenergy contaminants gene manipulation phytotechnology plant-microbe interaction regulatory policy soil pollu-tion soil remediation

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Photoluminescence emissions of Ca_(1-x)WO_(4):xEu^(3+):Bridging between experiment and DFT calculations

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Journal of Rare Earths 2022年第10期40卷 1527-1534页

作者： Amanda Fernandes Gouveia Marcelo Assis Lara Kelly Ribeiro Aline Estefany Brandao Lima eduardo de Oliveira Gomes Daniele Souza Yara Gobato Galvao Ieda Lucia Viana rosa Geraldo eduardo da Luz Jr Eva Guillamón Elson Longo Juan Andrés Miguel Angel San-Miguel Department of Physical and Analytical Chemistry University Jaume I(UJI)12071CastelloSpain Institute of Chemistry State University of Campinas(Unicamp)13083-970CampinasSPBrazil CDMF Federal University of S?o Carlos(UFSCar)P0.Box 67613565-905Sao CarlosSao PauloBrazil Departamento de Química Federal University of Piauí(UFPI)64049-550TeresinaPiautBrazil Physics Department Federal University of S?o Carlos(UFSCar)P.0.Box 67613565-905Sao CarlosSao PauloBrazil

In this work,the impact of the doping process on the photoluminescence emission of CaWO_(4) as a function of the concentration of Eu^(3+) cation(0.01 mol%,0.02 mol%,0.04 mol%,0.06 mol%,0.08 mol%,and 0.10 mol%) is discussed in ***_(1-x)WO~4:xEu^(3+) samples were successfully synthesized by a simple coprecipitation method followed by microwave *** blue shift in the absorption edge confirms the quantum confinement effect and the band gap energy covers the range from 3.91 to 4.18 eV,as the amount of Eu^(3+) cations *** experimental results are sustained by first-principles calculations,at the density functional theory level,to decipher the geometry and electronic properties,thereby enabling a more accurate and direct comparison between theory and experiment for the Ca_(1-x)WO_(4):xEu^(3+) structure.

关键词： Ca_(1-x)WO_(4):xEu^(3+) DFT calculations Photoluminescence emissions Rare earths

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Minimum inhibitory but maximum non-hemolytic concentration of plasma-treated catheters coated with *** and *** extracts

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Plasma Science and Technology 2023年第12期25卷 83-96页

作者： Francisco Javier ALONSO-MONTEMAYOR Miriam Desirée DÁVILA-MEDINA Alejandro ZUGASTI-CRUZ rosa Idalia NARRO-CÉSPEDES María Guadalupe NEIRA-VELÁZQUEZ AidéSÁENZ-GALINDO eduardo ALONSO-CAMPOS Autonomous University of Coahuila Saltillo 25280Mexico Center for Research in Applied Chemistry Saltillo 25294Mexico University of Monterrey San Pedro Garza García 66238Mexico

Antibacterial extract-coated catheters are promising alternatives to their conventional counterparts,but their hemocompatibility and thermal stability must be *** bacteria have developed resistance to conventional ***,the minimum inhibitory but non-hemolytic concentration(MIC-NH)and the thermal stability of Larrea tridentata(***)and Origanum vulgare(***)extract-coated catheters were ***,plasma pretreatment was performed to enhance the extract ***,the extract-coated catheters prevent Staphylococcus aureus colonization without causing hemolysis by using *** and *** extracts at MIC-NH(5000 and 2500μg ml~(-1),respectively).Moreover,it has been discovered that the extract coating and plasma treatment improved the thermal stability and the extract adhesion,***,this study provides evidence of alternative antibacterial but non-hemolytic extract-coated catheters.

关键词： antibacterial activity biocompatibility blood-contact device cold plasma nosocomial bacteria

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Arabidopsis NahG Plants as a Suitable and Efficient System for Transient Expression using Agrobacte#um tumefaciens

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Molecular Plant 2017年第2期10卷 353-356页

作者： Tábata rosas-Díaz Pepe Cana-Quijada Vitor Amorim-Silva Miguel A. Botella rosa Lozano-Durán eduardo R. Bejarano Shanghai Center for Plant Stress Biology (PSC) Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Shanghai 201602 China

Dear Editor Research in molecular plant biology has largely benefitted from technologies allowing exogenous gene expression. While the generation of stable transgenic lines is required in some cases, transient transfo... 详细信息

关键词： Suitable Transient tumefaciens

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