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Ref.rence genes f.r quantitative RT-PCR data in gastric tissues and cell lines

World Journal of.Gastroenterology 2013年第41期19卷 7121-7128页

作者： f.rnanda Wisnieski Danielle Queiroz Calcagno Mariana f.rreira Leal Leonardo caires dos Santos Carolina de Oliveira Gigek Elizabeth Suchi Chen Thaís Brilhante Pontes Paulo Pimentel Assumpo Mnica Barauna de Assumpo Smia Demachki Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith Disciplina de Genética Departamento de Morfologia e GenéticaUniversidade Federal de So PauloSo Paulo 04023900Brazil Departamento de Ortopedia e Traumatologia Universidade Federal de So PauloSo Paulo 04038031Brazil Núcleo de Pesquisa em Oncologia Hospital Joo de Barros BarretoUniversidade Federal do ParáBelém 60673000Brazil Laboratório de Citogenética Humana Instituto de Ciências BiológicasUniversidade Federal do ParáBelém 66075110Brazil

AIM:To evaluate the suitability of.ref.rence genes in gastric tissue samples and cell ***:The suitability of.genes ACTB,B2M,GAPDH,RPL29,and 18S rRNA was assessed in21 matched pairs of.neoplastic and adjacent nonneoplastic gastric tissues f.om patients with gastric adenocarcinoma,27 normal gastric tissues f.om patients without cancer,and 4 cell lines using reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR).The ranking of.the best single and combination of.ref.rence genes was determined by Normf.nder,geNorm,BestKeeper,and *** addition,GenEx sof.ware was used to determine the optimal number of.ref.rence *** validate the results,the mRNA expression of.a target gene,DNMT1,was quantif.ed using the dif.erent ref.rence gene combinations suggested by the various sof.ware packages f.r ***:ACTB was the best ref.rence gene f.r all gastric tissues,cell lines and all gastric tissues plus cell ***+B2M or ACTB+B2M was the best combination of.ref.rence genes f.r all the gastric *** the other hand,ACTB+B2M was the best combination f.r all the cell lines tested and was also the best combination f.r analyses involving all the gastric tissues plus cell *** to the GenEx sof.ware,2 or 3 genes were the optimal number of.ref.rences genes f.r all the gastric *** relative quantif.cation of.DNMT1 showed similar patterns when normalized by each combination of.ref.rence *** level of.expression of.DNMT1 in neoplastic,adjacent non-neoplastic and normal gastric tissues did not dif.er when these samples were normalized using GAPDH+B2M(P=0.32),ACTB+B2M(P=0.61),or GAPDH+B2M+ACTB(P=0.44).CONCLUSION:GAPDH+B2M or ACTB+B2M is the best combination of.ref.rence gene f.r all the gastric tissues,and ACTB+B2M is the best combination f.r the cell lines tested.

关键词： Gastric cancer Ref.rence gene Normalization Gene expression Quantitative real-time polymerase chain reaction

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Quality of.Lif. and Osteoporotic Alterations in Patients with and without Low Bone Mineral Density:A Retrospective Study

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Journal of.Health Science 2019年第1期7卷 10-16页

作者： Christyan Hiroshi Iida Natália Isis caires Lavor Luciana Munhoz Reinaldo Abdala Júnior Rogério Abdala Emiko Saito Arita Department of.Stomatology School of Dentistry University of Sao Paulo 2227 Lineu Prestes Avenue Sao Paulo 05508-000 SP Brazil CDB(Centro de Diagnoticos Brasil) Rua Marselhesa 500-Vila Mariana Sao Paulo SP Brazil

Purpose:The objectives of.this investigation were to compare the World Health Organization abbreviated instrument f.r quality of.lif. assessment(WHOQOL-bref.scores of.osteoporotic and non-osteoporotic Brazilian patients and to evaluate the correlation between mandibular cortical index(MCI)and bone densitometry(DXA)results in both ***:This research was conducted with an initial sample of.74 f.male participants who underwent panoramic radiography and f.rearm *** were divided into two groups:osteoporotic and non-osteoporotic patients.A quality of.lif. assessment questionnaire was *** scores f.om the questionnaire were compared between the two groups and the correlation between DXA and MCI was ***:Psychological and social domains in osteoporotic patients presented lower scores(12.65 and 6.00 respectively)when compared to non-osteoporotic patients(14.70 and 13.97 respectively).MCI was inversely correlated with ***:According to WHOQOL-bref.osteoporotic patients may have lower quality of.lif. scores when considering psychological and social *** is inversely correlated with T-scores but demonstrates greater correlation when considering non-osteoporotic patients with higher T-score values.

关键词： Osteoporosis panoramic radiography bone loss quality of lif. bone mineral density

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Prevalence of.anal human papillomavirus inf.ction in patients with human immunodef.ciency virus inf.ction:A systematic review

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World Journal of.Clinical Inf.ctious Diseases 2021年第3期11卷 38-48页

作者： Cinara Dourado Pereira Correia Santos Caio caires Brandão f.rnanda Santos Mota Isis Souza f.rreira Caline Novais Teixeira Oliveira Cláudio Lima Souza f.brício f.eire de Melo Márcio Vasconcelos Oliveira Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Medical School Faculdade Santo AgostinhoVitória da Conquista 45028-100BahiaBrazil

BACKGROUND Human papillomavirus(HPV)is one of.the most common sexually transmitted viruses *** To analyze the prevalence of.HPV inf.ction in human immunodef.ciency virus(HIV)-positive patients and the risk f.ctors associated with this inf.ction through a review of.studies published in the period f.om January 2010 to April *** A total of.384 articles were initially identif.ed in our searches,of.which ten were selected according to previously def.ned eligibility *** Anal intercourse,absence of.condom use,multiple partners,other specif.c sexual and lif. habits,and HIV inf.ction are among the risk f.ctors associated with anal HPV *** In general,there is a higher prevalence of.anal HPV inf.ction among HIV-positive patients,mostly in individuals over 30 years old,those with multiple partners,those who had an early homosexual debut,and cigarette,alcohol,and drug users.

关键词： Human immunodef.ciency virus Anal human papillomavirus Risk f.ctors Systematic review

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