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Comparability Assessments of Process Changes Made during Development of Anti-Idiotype Vaccine

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World Journal of Vaccines 2014年第1期4卷 24-32页

作者： Kathya de la Luz-Hernández Yamilé Rabasa Raquel Montesinos Dasha Fuentes Julio Felipe Santo Tomás Orlando Morales Yadira Aguilar Blanca pacheco Adolfo Castillo Ana Maria Vazquez Center of Genetic Engineering and Biotechnology Havana Cuba national Center for Laboratory Animal Breeding Havana Cuba Process Development Direction INIM Center of Molecular Immunology Havana Cuba

Racotumomab monoclonal antibody is a murine anti-idiotypic antibody. This monoclonal antibody mimics n-glycolyl-GM3 gangliosides has been tested in several clinical trials Phase I/II for breast, melanoma and non-small cell lung cancer patients as an anti-idiotypic cancer vaccine. The early production process was performed in vivo from mice ascites fluid. This process was transferred to bioreactor-based method at pilot scale followed to the scale-up of the fermentation. In this work we present a comprehensive molecular characterization of racotumomab MAb produced by the two different production scales in order to determine the impact of the manufacturing process in vaccine performance. We observed differences in glycosylation pattern and charge heterogeneity between racotumomab produced in both scales. Interestingly, these modifications had no significant impact on biological activity elicited in chickens. So, changes in primary structure like glycosylation, charge heterogeneity and oxidation did not affect biological activity of the vaccine.

关键词： Comparability Studies Monoclonal Antibody Cancer Vaccine Mass Spectrometry

Turning, Microbial Inoculation and Use of Eisenia foetida in Household Organic Waste Composting

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Journal of Agricultural Science and Technology(A) 2011年第5期1卷 734-738页

作者： I. lrigoyen F. pacheco M. Sesma I. Domeno F. Storino J. Muro B. Yaben S. Blazquez A. Amorena Department of Produccion Agraria Universidad Publica de Navarra Pamplona 31006 Spain Servicios de la Comarca de Pamplona SCPSA c/General Chinchilla n7 Pamplona 31002 Spain

On site composting of organic household wastes is an economical and environmentally friendly way to manage municipal wastes. In this manuscript authors evaluated the importance of turning the wastes and of inoculating microorganisms and worms in order to improve the composting process at domestic scale. Four treatments (Control without turning– C-, Control with Turning– CT-, inoculated Mountain Microorganism with turning– MM- and Worms without turning– W-) were tested in a random experiment with four replicates. Sixteen composting bins were fed with 300 kg of organic wastes from a local street market. The process of composting lasted 13 weeks after which all the composts were sifted and submitted to a range of chemical, physical and biological analysis. According to the results MM slightly increased the initial temperature and enzymatic activity. This investigation outcome don’t provide of sufficient grounds for a precise recommendation about worms inoculation in compost bins. A decrease between 75% and 80% in the fresh weight of the organic waste was found in the first thirteen weeks after starting the composting process in all the treatments. The quality level of the compost was acceptable, with very low heavy metal content. Turn over helps to keep the product hygienic especially after the inoculation with MM microbial starts. In conclusion and according to the results of this investigation, the use of 320 L compost bins for the organic waste management is strongly advisable.

关键词：微生物接种垃圾堆肥有机废物赤子爱胜蚓车削家用堆肥过程废物管理

Microstructure,mechanical response and fractography of AZ91E/Al_(2)O_(3)_(p) nano composite fabricated by semi solid stir casting method

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Journal of Magnesium and Alloys 2017年第1期5卷 48-55页

作者： Sameer Kumar D Suman K.n.S Tara Sasanka C Ravindra K Palash Poddar Department of Mechanical Engineering R.V.R.&J.C.College of EngineeringGunturIndia Department of Mechanical Engineering Andhra University College of EngineeringVisakhapatnamIndia CSIR-national Metallurgical Laboratory JamshedpurIndia d Department of Mechanical EngineeringNarasaraopeta Engineering CollegeNarasaraopetIndia

The present study confers to the fabrication and its characterization of magnesium alloy(AZ91E)based nano composites with nano Al_(2)O_(3) particulate reinforcements.A novel Semi Solid stir casting technique was adopted for the fabrication of the *** average particle size of 50 nm was used as reinforcement to disperse in *** effects of change in weight fraction of reinforcements on the distribution of particles,particle–matrix interfacial reactions,physical as well as mechanical properties were *** SEM and eds analysis has shown the uniform distribution of particles in the composite along with the presence of *** mechanical properties of reinforced and unreinforced composite were evaluated and *** of tensile specimens was also discussed.

关键词： Magnesium alloys nano composites Semi solid stir casting Al_(2)O_(3)particle

Theoretical prediction,synthesis,and crystal structure determination of new MAX phase compound V2SnC

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Journal of Advanced Ceramics 2020年第4期9卷 481-492页

作者： Qiang Xu Yanchun Zhou Haiming Zhang Anna Jiang Quanzheng Tao Jun Lu Johanna Rosén Yunhui niu Salvatore Grasso Chunfeng Hu Key Laboratory of Advanced Technologies of Materials Ministry of EducationSchool of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu610031China Science and Technology on Advanced Functional Composite Laboratory Aerospace Research Institute of Materials&Processing TechnologyBeijing100076China School of Mechanical Electronic and Control EngineeringBeijing Jiaotong UniversityBeijing100044China Thin Film Physics Division Department of PhysicsChemistryand Biology(IFM)Linköping UniversityLinköpingSE-58183Sweden State Key Laboratory of Environment-friendly Energy Materials Southwest University of Science and TechnologyMianyang621010China

Guided by the theoretical prediction,a new MAX phase V2SnC was synthesized experimentally for the first time by reaction of V,Sn,and C mixtures at 1000°*** chemical composition and crystal structure of this new compound were identified by the cross-check combination of first-principles calculations,X-ray diffraction(XRD),energy dispersive X-ray spectroscopy(eds),and high resolution scanning transmission electron microscopy(HR-STEM).The stacking sequence of V2C and Sn layers results in a crystal structure of space group P63/*** a-and c-lattice parameters,which were determined by the Rietveld analysis of powder XRD pattern,are 0.2981(0)nm and 1.3470(6)nm,*** atomic positions are V at 4f(1/3,2/3,0.0776(5)),Sn at 2d(2/3,1/3,1/4),and C at 2a(0,0,0).A new set of XRD data of V2SnC was also *** calculations suggest that this new compound is stable with negative formation energy and formation enthalpy,satisfied Born-Huang criteria of mechanical stability,and positive phonon branches over the Brillouin *** also has low shear deformation resistance c44(second-order elastic constant,cij)and shear modulus(G),positive Cauchy pressure,and low Pugh’s ratio(G/B=0.500nism underpinning the quasi-ductility is associated with the presence of a metallic bond.

关键词： V2SnC new MAX phase compound crystal structure first-principles calculations

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一种复杂的余震序列模型

世界地震译丛 2003年第1期34卷 1-12页

作者： Y.Moreno A.M.Correig J.B.Gomez A.F.pacheco 邵辉成陕西省地震局

通常,修正的大森定律 n(t)∝t^(-p)能很好地描述余震的衰减速率,其中n(t)表示单位时间的余震数,t 是主震后的时间,p 为0.9～1.5之间并通常接近于1的常数。然而,也存在一些更复杂的余震序列,大森定律只能被用作其一级近似。1996年2月18... 详细信息

通常,修正的大森定律 n(t)∝t^(-p)能很好地描述余震的衰减速率,其中n(t)表示单位时间的余震数,t 是主震后的时间,p 为0.9～1.5之间并通常接近于1的常数。然而,也存在一些更复杂的余震序列,大森定律只能被用作其一级近似。1996年2月18日发生在东比利牛斯地震的余震序列就是这些复杂余震系列中的一个,correig 等(1997)对该地震进行过详细的描述。本文中,我们受动态纤维束模型的启发而提出了一种新的模型,用来解释余震速率突然增加的这类复杂的余震序列,而速率的增加与余震的震级无直接关系(如传染型余震序列)。这是一种简单、离散和随机破裂的模型,它的单元(凹凸体或障碍体)会由于局部荷载共享规律而引起的静态疲劳和应力转移而破裂并又重新生成。我们发现这种模型与东比利牛斯余震序列非常一致,除岩石强度随时间变化的部分之外,我们认为余震的主要机制是存在动态应力的变化,而这种变化往往又为序列中下一个余震重新设置了初始条件。

关键词：余震序列模型应力岩石强度震级衰减速率

Genomic Analysis of 727 Patients with Ehlers-Danlos Syndrome I: Clinical Perspective Relates 23 Genes to a Maternally Influenced Arthritis-Adrenaline Disorder

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Journal of Biosciences and Medicines 2019年第12期7卷 181-204页

作者： Golder n. Wilson Texas Tech University Health Science Center Lubbock and Kinder Genome Genetics Private Practice Dallas TX USA

A novel medical approach for qualifying DnA variants found by whole exome sequencing (WES) facilitates discovery of new gene-disease relationships and emphasizes that DnA change must be correlated with clinical findings before having utility for diagnosis. Delineation of an arthritis-adrenaline disorder (AAD) process qualified variants in 23 genes as diagnostically useful in 727 patients having WES among 1656 with Ehlers-Danlos syndrome (eds);these results distinguished them from 102 patients who had qualified gene variants among 728 with developmental disability. Excess maternal transmission of AAD by pedigree analysis plus 167 maternally versus 111 paternally transmitted DnA variants and 75 patients with only mitochondrial DnA variants suggest maternal influence on inheritance of AAD and its subsumed eds types. Genes grouped by impact on different connective tissue elements showed variation in similar numbers of patients with hypermobile or classical eds, benign joint hypermobility, or predominant dysautonomia: COL7A1, FLG acting on skin in 21 patients;SCn9A/10A/11A, POLG on nerve in 24;COL6A1/A2/A3, COL12 on muscle in 19;COL5A1/A2, FBn1, TGFB2/3, TGFBR1/2 on tissue matrix in 51;COL3A1, VWF on vessel in 18;COL1A1/A2, COL11A1/A2 acting on bone in 15 patients. Each gene group acts through a postulated articulo-autonomic dysplasia cycle to produce reciprocal tissue laxity and dysautonomia findings that transcend eds types. This same tissue laxity-dysautonomia cycle acts to produce secondary complications in disorders ranging from distinctive connective tissue dysplasias to developmental disorders with hypotonia and acquired conditions with autonomic imbalance. Several altered genes were previously associated with neuromuscular disorders, foreshadowing a large myopathic eds category that will incorporate many patients with hypermobility. The importance of muscle for joint constraint supports present exercise and future mesenchymal stem cell therapies, whether AAD is g

关键词： Ehlers-Danlos Syndrome Connective Tissue Dysplasia Arthritis-Adrenaline Disorder Articulo-Autonomic Dysplasia Whole Exome Sequencing Collagen Genes

Clinical Analysis Supports Articulo-Autonomic Dysplasia as a Unifying Pathogenic Mechanism in Ehlers-Danlos Syndrome and Related Conditions

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Journal of Biosciences and Medicines 2019年第6期7卷 149-168页

作者： Golder n. Wilson Texas Tech University Health Science Center Lubbock and KinderGenome Medical Genetics Private Practice Dallas TX USA

Findings in 1656 patients referred for evaluation of Ehlers-Danlos syndrome, 710 evaluated systematically using novel history and physical forms, defined a characteristic clinical pattern termed arthritis-adrenaline disorder, a genus that provides immediate therapy while delineation of particular tissue laxity/dysautonomia species is underway. Preliminary diagnoses, clinical findings, and laboratory results were entered into an MS Excel? database with IRB approval and correlations or statistical significance analyzed using Excel? functions. Frequencies of 80 findings by history and 40 on physical were similar among eds groups, females paralleling males with more total history (35 versus 23) and physical (18 versus 15) findings. Finding frequencies in joint-skeletal (6.2 of 15) and dysautonomia (11 of 20) subcategories were substantial regardless of age, eds diagnosis, or referral source, the latter was shown by 6.4 and 13 average findings for cardiology, 5.3 and 8.3 for orthopedic referrals. Early affliction evidenced by history findings averaging 19.5 in those under 12 increased dramatically to 25 for teens and 32 for adults with plateauing at older ages arguing against degenerative disease. Frequent neuromuscular symptoms in females emphasize surrounding muscle support and protection of joint-connective tissue as a key factor in decreased male severity. The congruent clinical profile suggests operation of an articulo-autonomic dysplasia cycle where lax vessels and lower body pooling elicit sympathetic response, autonomic imbalance in turn affecting small nerve fibers and enhancing connective tissue laxity. Recognition of this arthritis-adrenalin disorder can guide management strategies while underlying causes are pursued, among them, physical therapy, exercise, and vitamin D to build muscle/bone strength;lower gluten/dairy and antihistamine protocols for low bowel motility/mast-cell activation;hydration, salt, and exercise for postural orthostatic tachycardia synd

关键词： Ehlers-Danlos Syndrome Arthritis-Adrenaline Disorder Connective Tissue Dysplasia Hypermobility Dysautonomia Joint Laxity Skin Elasticity IBS POTS MCAD

硼硅酸盐生物玻璃表面多层结构的形成机理(英文)

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无机化学学报 2008年第7期24卷 1132-1136页

作者：姚爱华林健段祥黄文旵 Rahaman Mohamed n 同济大学材料科学与工程学院上海200092 Missouri University of Science and Technology MOUSA65409

描述了硼硅酸盐生物活性玻璃在体外含磷溶液中的转变过程,并采用XRD、SEM和eds对反应产物的微观结构、形貌和成分进行了分析。结果显示,产物为多层结构,由羟基磷灰石和无定型SiO2层交替排列而成。此外,提出了一个定性模型来解释层状结... 详细信息

描述了硼硅酸盐生物活性玻璃在体外含磷溶液中的转变过程,并采用XRD、SEM和eds对反应产物的微观结构、形貌和成分进行了分析。结果显示,产物为多层结构,由羟基磷灰石和无定型SiO2层交替排列而成。此外,提出了一个定性模型来解释层状结构的形成机制。研究证实,反应动力学及反应产物的微观结构主要取决于生物活性玻璃的成分和含磷溶液的浓度。

关键词：硼硅酸盐玻璃羟基磷灰石形成机理生物活性玻璃

Synthesis and Characterization of Green Potassium nanoparticles from Sideroxylon Capiri and Evaluation of Their Potential Antimicrobial

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Journal of Renewable Materials 2021年第10期9卷 1699-1706页

作者： Judith Constantino-Alcazar Miguel Abud-Archila Benjamín Valdez-Salas Federico Gutierrez-Miceli Carlos Ceceña-Duran Blanca López-Valenzuela Daniel Gonzalez-Mendoza Tecnológico nacional de México Instituto Tecnológico de Tuxtla GutiérrezTuxtlaGutiérrezC.P.03940México Instituto de Ciencias Agrícolas de la Universidad Autónoma de Baja California(ICA-UABC) Ejido Nuevo LeónC.P.21705México Instituto de Ingeniería de la Universidad Autónoma de Baja California Mexicali21100México

In the present study,the green synthesis of potassium nanoparticles(K-nPs)was assessed using aqueous extract of Sideroxylon *** potassium nanoparticles were analyzed by UV-visible spectroscopic techniques,X-ray spectrometers of energy dispersive(SEM-eds)and dynamic light *** results showed high values at 3.5 keV confirming the formation of potassium nanoparticles and the SEM analysis showed an agglomerated particles size between 360 to 200 nm with a spherical *** K-nPs showed an effective antibacterial activity against the test organisms mainly with Bacillus cereus,Enterobacter aerogenes,Fusarium solani and Botrytis *** further studies about nanotoxicity of K-nPs are needed to confirm their potential in the control of the pathogen microorganisms under field conditions.

关键词： Antimicrobial effect green synthesis Sideroxylon capiri biocontrol