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Proteomic signatures of infertile men with clinical varicocele and their validation studies reveal mitochondrial dysfunction leading to infertility

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Asian Journal of Andrology 2016年第2期18卷 282-291,J0007页

作者： Ashok Agarwal Rakesh Sharma Luna Samanta Damayanthi Durairajanayagam edmund sabanegh American Center for Reproductive Medicine Cleveland Clinic Cleveland OH USA Department of Zoology School of Life Sciences Ravenshaw UniversityCuttack - 751 003 Odisha India Faculty of Medicine MARA University of Technology (UiTM) Selangor Darul Ehsan Malaysia Department of Urology Cleveland Clinic Cleveland OH USA.

To study the major differences in the distribution of spermatozoa proteins in infertile men with varicocele by comparative proteomics and validation of their level of expression. The study-specific estimates for each varicocele outcome were combined to identify the proteins involved in varicocele-associated infertility in men irrespective of stage and laterality of their clinical varicocele. Expression levels of 5 key proteins （PKARIA, AK7, CCT6B, HSPA2, and ODF2） involved in stress response and sperm function including molecular chaperones were validated by Western blotting. Ninety-nine proteins were differentially expressed in the varicocele Eroup. Over 87% of the DEP involved in major energy metabolism and key sperm functions were underexpressed in the varicocele group. Key protein functions affected in the varicocele group were spermatogenesis, sperm motility, and mitochondrial dysfunction, which were further validated by Western blotting, corroborating the proteomics analysis. Varicocele is essentially a state of energy deprivation, hypoxia, and hyperthermia due to impaired blood supply, which is corroborated by down-regulation of lipid metabolism, mitochondrial electron transport chain, and Krebs cycle enzymes. To corroborate the proteomic analysis, expression of the 5 identified proteins of interest was validated by Western blotting. This study contributes toward establishing a biomarker ＂fingerprint＂ to assess sperm quality on the basis of molecular parameters.

关键词： bioinformatics male infertility proteomics spermatozoa proteins varicocele

46 XX karyotype during male fertility evaluation; case series and literature review

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Asian Journal of Andrology 2017年第2期19卷 168-172页

作者： Ahmad Majzoub Mohamed Arafa Christopher Starks Haitham Elbardisi Sami A1 Said edmund sabanegh Jr Cleveland Clinic Foundation Department of Urology Cleveland Ohio USA Hamad Medical Corporation Department of Urology Qatar Cairo University Department of Andrology Giza Egypt Reston Hospital Department of Urology Virginia USA

Forty-six XX disorder of sex development is an uncommon medical condition observed at times during the evaluation of a man＇s fertility. The following is a case series and literature review of phenotypically normal men diagnosed with this karyotype. Our goal is to comprehend the patients＇ clinical presentation as well as their laboratory results aiming to explore options available for their management. A formal literature review through PubMed and MEDLINE databases was performed using ＂46 XX man＂ as a word search. A total of 55 patients, including those conveyed in this article were diagnosed with a 46 XX karyotype during their fertility evaluation. The patients＇ mean age _＋ s.d. was 34 ＋ 10 years and their mean height ＋ s.d. was 166 ＋ 6.5 cm. Overall, they presented with hypergonadotropic hypogonadism. Sexual dysfunction, reduced hair distribution, and gynecomastia were reported in 20% （4120）, 25.8% （8/31）, and 42% （13131） of the patients, respectively. The SRYgene was detected in 36 （83.7%） and was absent in the remaining seven （16.3%） patients. We found that a multidisciplinary approach to management is preferred in 46 XX patients. Screening for remnants of the mullerian ducts and for malignant transformation in dysgenetic gonads is imperative. Hypogonadism should be addressed, while fertility options are in vitro fertilization with donor sperm or adoption.

关键词： hypogonadism infertility male sex-determining region XX disorders of sex development

Spermatozoa protein alterations in infertile men with bilateral varicocele

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Asian Journal of Andrology 2016年第1期18卷 43-53页

作者： Ashok Agarwal Rakesh Sharma Damayanthi Durairajanayagam Zhihong Cui Ahmet Ayaz Sajal Gupta Belinda Willard Banu Gopalan edmund sabanegh Center for Reproductive Medicine Glickman Urological and Kidney Institute Cleveland Clinic Cleveland OH USA Faculty of Medicine MARA University of Technology Selanger Malaysia Lerner Research institute Cleveland Clinic Cleveland OH USA

Among infertile men, a diagnosis of unilateral varicocele is made in 90% of varicocele cases and bilateral in the remaining varicocele cases. However, there are reports of under-diagnosis of bilateral varicocele among infertile men and that its prevalence is greater than 10%. In this prospective study, we aimed to examine the differentially expressed proteins （DEP） extracted from spermatozoa cells of patients with bilateral varicocele and fertile donors. Subjects consisted of 17 men diagnosed with bilateral varicocele and 10 proven fertile men as healthy controls. Using the LTQ-orbitrap elite hybrid mass spectrometry system, proteomic analysis was done on pooled samples from 3 patients with bilateral varicocele and 5 fertile men. From these samples, 73 DEP were identified of which 58 proteins were differentially expressed, with 7 proteins unique to the bilateral varicocele group and 8 proteins to the fertile control group. Majority of the DEPs were observed to be associated with metabolic processes, stress responses, oxidoreductase activity, enzyme regulation, and immune system processes. Seven DEP were involved in sperm function such as capacitation, motility, and sperm-zona binding. Proteins TEKT3 and TCP11 were validated by Western blot analysis and may serve as potential biomarkers for bilateral varicocele. In this study, we have demonstrated for the first time the presence of DEP and identified proteins with distinct reproductive functions which are altered in infertile men with bilateral varicocele. Functional proteomic profiling provides insight into the mechanistic implications of bilateral varicocele-associated male infertility.

关键词： bilateral varicocele bioinformatics male infertility proteomics spermatozoa proteins varicocele

Asymptomatic postpubertal male with equally sized normal testicles with palpable left varicocele

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Asian Journal of Andrology 2016年第2期18卷 310-310页

作者： A Scott Polackwich edmund sabanegh Jr Male Infertility and Microsurgery Department of Urology Glickman Urological and Kidney Institute Cleveland Clinic Foundation 9500 Euclid Avenue QIOCleveland OH 44195 USA

Varicoceles are a common finding in the general public. The incidence appears to increase later in life with a contemporary study finding an increase in incidence of 10% per decade of life） While common in the general population, they are even more common among those presenting for a fertility evaluation, as high as 39% in some series and are often initially diagnosed during such examinations） Others present without symptoms or fertility issues and are found incidentally on a physical exam by a general practitioner.

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Study on the Microsecond Pulse Homogeneous Dielectric Barrier Discharges in Atmospheric Air and Its Influencing Factors

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Plasma Science and Technology 2011年第6期13卷 676-681页

作者：方志雷枭蔡玲玲邱毓昌 edmund KUFFEL School of Automation and Electrical Engineering Nanjing University of Technology State Key Laboratory of Electrical Insulation and Power Equipment xi'an Jiaotong University Department of Electrical and Computer Engineering University of Manitoba

The homogeneous dielectric barrier discharge （DBD） in atmospheric air between two symmetric-columnar copper electrodes with epoxy plates as the dielectric barriers is generated using a us pulse high voltage power supply. The discharge characteristics are studied by measurement of its electrical discharge parameters and observation of its light emission phenom- ena, and the main discharge parameters of the homogenous DBD, such as discharge current and average discharge power, are calculated. Results show that the discharge generated is a homogeneous one with one larger single current pulse of about 2 #s duration appearing in each voltage pulse, and its light emission is radially homogeneous and covers the entire surface of the two elec- trodes. The influences of applied voltage amplitude, air gap distance and barrier thickness on the transition of discharge modes are studied. With the increase of air gap distance, the discharge will transit from homogeneous mode to filamentary mode. The higher the thickness of dielectric barriers, the larger the air gap distance for generating the homogeneous discharge mode. The average discharge power increases non-linearly with increasing applied voltage amplitude, and decreases non-linearly with the increase of air gap distance and barrier thickness. In order to generate stable and homogeneous DBD with high discharge power, thin barriers distance should be used, and higher applied voltage amplitude should be applied to small air gap.

关键词： dielectric barrier discharge (DBD) homogeneous mode filamentary mode discharge characteristics influencing factors

Nicotinic acetylcholine signaling is required for motor learning but not for rehabilitation from spinal cord injury

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Neural Regeneration Research 2023年第2期18卷 364-367页

作者： Yue Li edmund R.Hollis II Burke Neurological Institute White PlainsNYUSA Feil Family Brain and Mind Research Institute Weill Cornell MedicineNew YorkNYUSA Current address:Department of Neuroscience University of Rochester Medical CenterRochesterNYUSA

Therapeutic intervention for spinal cord injury is limited,with many approaches relying on strengthening the remaining substrate and driving recovery through rehabilitative *** compared with learning novel compensatory strategies,rehabilitation focuses on resto ring movements lost to *** rehabilitation of previously learned movements after spinal cord injury requires the molecular mechanisms of motor learning,or if it engages previously trained motor circuits without requiring novel learning remains an open *** this study,mice we re randomly assigned to receive intrape ritoneal injection with the pan-nicotinic,non-competitive antagonist mecamylamine and the nicotinicα7 subunit selective antagonist methyllycaconitine citrate salt or vehicle(normal saline)prior to motor learning assays,then randomly reassigned after motor learning for rehabilitation study *** rvical spinal co rd dorsal column lesion was used as a model of in complete *** of this study showed that nicotinic acetylcholine signaling was required for motor learning of the single pellet-reaching task but it was dispensable for the rehabilitation of the same task after *** findings indicate that critical diffe rences exist between the molecular mechanisms supporting compensatory motor learning strategies and the restoration of behavior lost to spinal cord injury.

关键词： acetylcholine basal forebrain corticospinal tract dorsal column lesion mecamylamine methyllycaconitine motor control rehabilitation rotarod single pellet-reaching task

DYNAMIC MODELLING OF BAR-GEAR MIXED MULTIBODY SYSTEMS USING A SPECIFIC FINITE ELEMENT METHOD

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Chinese Journal of Mechanical Engineering 1999年第2期12卷 12-18页

作者： Wang, Yong Cheung, Hongman edmund Zhang, Wenjun City Univ of Hong Kong Hong Kong Hong Kong

A new dynamic model for mixed, flexible bar and gear multibody systems is developed based on a specific finite element method, and a new gear element is proposed. The gear element can take into account the time variant stiffness, the gear errors and mass unbalance. The model for geared multibody systems can couple the gear meshing and the flexibility of all contained components. The kinematic and dynamic analyses of the geared multibody systems are expounded and illustrated on an example composed of three gears, two bars and one slider.

关键词： Gear Finite element Multibody system Simulation Dynamics

An evaluation of a perfluoromethylcyclohexane(PMCH) permeation plug release vessel(PPRV) in a controlled turbulent environment

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International Journal of Mining Science and Technology 2015年第2期25卷 243-251页

作者： Jong edmund C. Luxbacher Kray D. Department of Mining and Minerals Engineering Virginia Tech

The use of sulfur hexafiuoride （SF6） as a tracer gas for analyzing underground mine ventilation systems has been practiced for over 30 years. As a result, the methods used to release, sample, and analyze SF6 are well accepted. As the complexity and size of underground mine ventilation networks increase, the ability of a SF6 to function as a convenient and rapid means of analysis diminishes. The utilization of multiple tracer gases can mitigate this by removing the need to purge the background presence of a tracer before conducting another release and allowing for a more comprehensive evaluation using multi-zone tech- niques. Recent studies have identified perfluoromethylcyclohexane （PMCH） as a possible supplement for SF6 in underground mine ventilation tracer studies. However, the deployment of PMCH remains a challenge because of this compounds physical properties. This paper evaluates a PMCH permeation plug release vessel （PPRV） under controlled turbulent conditions. The details of the experimental parameters used in the evaluation as well as a discussion regarding the performance of the PPRV are included.

关键词： Tracer gas Underground mines Mine ventilation Perfluoromethylcyclohexane

Enhancement and validation of building integrated PV system:3D modelling,techno-economics and environmental assessment

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Energy and Built Environment 2022年第4期3卷 444-466页

作者： edmund Jin Wen Thoy Yun Ii Go School of Engineering and Physical Sciences Heriot-Watt University Malaysia.1Jalan Venna P5/2Precinct 562200 PutrajayaWilayah Persekutuan PutrajayaMalaysia

The incorporation of photovoltaic elements in buildings have been gaining more mileage in recent *** integrated photovoltaic(BIPV)technologies are on the rise in terms of efficiency and longevity,with a compound annual growth rate(CAGR)of 15.7%since *** costs of production and raw materials of BIPV have reached a level that is economically beneficial for building developers to adopt the ***,the lack of infras-tructure as compared to traditional means of energy production has impeded the maturing of such *** issue of conversion efficiency and module degradation have been addressed but not completely resolved by the scientific *** attractive governmental incentives such as net energy metering(NEM)and better returns of investments are shifting the tide as of late,what remains to be seen is the mass adoption of BIPV technology in residential and commercial *** work aims to develop an optimal layout for photo-voltaic panels in the university building precise 3D modelling and solar energy resource *** method adopted is based on energy production capability of a 3D modelled BIPV system which will be carried out in three *** are i)Assessment of geographical location and meteorological data,ii)Development of 3D model and orientation analytics and(iii)Development of optimal PV *** systems were considered for this study,which is the roof and two systems on the Southern Faç*** proposed rooftop BIPV design is expected to provide 49.27%of the building’s energy consumption while reducing CO_(2)emissions by 20155.32 tonnes throughout the lifespan of the system’s *** paper serves as a pioneering study on the feasibility of a BIPV system through the incorporation of building geometry for computations on incident solar *** the reduction of electricity imported from the grid,the adoption of the BIPV system also serves as an incremental step towards achi

关键词： Facade Energy yield Performance ratio Payback Building geometry