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Buzz, the Terror of the Forest

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英语大王 2014年第3期 6-9页

作者： Dobos edit

It was a lovely autumn morning."What a warm sunny day!"Buzz thought."Let’s go outside(外面)!"Her friends are still sleeping,so the small bee set off on her own.*She was very happy and started*humming(哼唱)a song.

关键词：小助手 the Terror of the Forest

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Two Dogs

英语大王 2014年第Z2期 12-17页

作者： Dobos edit

Presto the wizard(巫师)ran into the room with his magic ball and said,"I saw him!I saw Paws!"*The others(其他人)were very excited*.They came to look into the magic ball."What are you looking for*in my ball?"asked Presto."Paws,of course*!"they answered."In my magic ball?But I saw him through(通过)the window."Paws walked through the door."Paws,we are so glad to see you!"Everyone was very happy.

关键词： Two Dogs

Multi-exponential model to describe pressure-dependent P-and S-wave velocities and its use to estimate the crack aspect ratio

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Journal of Rock Mechanics and Geotechnical Engineering 2022年第2期14卷 385-395页

作者： Mihály Dobróka Norbert Péter Szabó Tünde edit.Dobróka Mátyás Krisztián Baracza Department of Geophysics University of MiskolcMiskolc-Egyetemváros3515Hungary MTA-ME Geoengineering Research Group University of MiskolcMiskolc-Egyetemváros3515Hungary Research Institute of Applied Earth Sciences University of MiskolcMiskolc-Egyetemváros3515Hungary

We present new quantitative model describing the pressure dependence of acoustic P-and S-wave *** that a variety of individual mechanisms or defects(such as cracks,pore collapse and grain crushing)can contribute to the pressure-dependent change of the wave velocity,we order a characteristic pressure to all of them and allow a series of exponential terms in the description of the(Pand S-waves)velocity-pressure *** estimate the parameters of the multi-exponential rock physical model in inversion procedures using laboratory measured P-and S-wave velocity *** is known,the conventional damped least squares method gives acceptable results only when one or two individual mechanisms are *** the number of exponential terms leads to highly nonlinear ill-posed inverse *** to this reason,we develop the spectral inversion method(SIM)in which the velocity amplit.des(the spectral lines in the characteristic pressure spectrum)are only considered as *** characteristic pressures(belonging to the velocity amplit.des)are excluded from the set of inversion unknowns,instead,they are defined in a set of fixed positions equidistantly distributed in the actual interval of the independent variable(pressure).Through this novel linear inversion method,we estimate the parameters of the multi-exponential rock physical model using laboratory measured P-and S-wave velocity *** characteristic pressures are related to the closing pressures of cracks which are described by well-known rock mechanical relationships depending on the aspect ratio of elliptical *** gives the possibilit. to estimate the aspect ratios in terms of the characteristic pressures.

关键词： Multi-exponential rock physical model Spectral inversion method(SIM) Crack aspect ratio Characteristic pressures

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CRISPR/Cas9编辑MeHNL基因创制低生氰糖苷木薯

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生物技术通报 2024年第9期40卷 11-19页

作者：童玮婧罗数陆新露沈建福陆柏益李开绵马秋香张鹏中国科学院分子植物科学卓越创新中心上海200032 中国科学院大学北京100049 浙江大学生物系统工程与食品科学学院杭州310058 中国热带农业科学院热带作物品种资源研究所海口571101

【目的】木薯(Manihot esculenta Crantz)中含有潜在毒性的生氰糖苷,其食用安全性受到影响且导致加工成本增加。因此,利用生物技术开展低生氰糖苷木薯的培育具有重要意义。【方法】利用CRISPR/Cas9技术对木薯醇氰酶基因MeHNL进行了编辑... 详细信息

【目的】木薯(Manihot esculenta Crantz)中含有潜在毒性的生氰糖苷,其食用安全性受到影响且导致加工成本增加。因此,利用生物技术开展低生氰糖苷木薯的培育具有重要意义。【方法】利用CRISPR/Cas9技术对木薯醇氰酶基因MeHNL进行了编辑。该基因编码催化生氰糖苷分解的α-羟基腈裂解酶,编辑靶点位于第1个外显子上,通过农杆菌介导的稳定转化获得了27株阳性植株。【结果】测序分析显示,其中26个株系被编辑,编辑效率高达96.3%。编辑类型主要包括碱基的插入和缺失,少数为碱基替换和大片段缺失。氰化物检测试剂盒染色和HPLC测定分析表明,编辑株系中的氢氰酸和生氰糖苷含量均显著降低。与非编辑植株相比,编辑植株的叶片细长,暗示了MeHNL可能对木薯的生长发育产生影响。【结论】利用CRISPR/Cas9技术获得了低氰化物的木薯种质,为开展生氰糖苷代谢影响木薯生长发育的研究提供了材料。

关键词：木薯基因编辑醇氰酶低氰化物生氰糖苷

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