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Advances in Microbiology 2017年第6期7卷 480-486页

作者： Ahmed Faruk Umar Fatimah Tahir ediga bede agbo Department of Microbiology Abubakar Tafawa Balewa University Bauchi Nigeria

The population dynamics of bacterial community was investigated in three Agricultural soils, designated as Loamy sand (A), Peaty coarse (B) and Loamy coarse sand (C) in North-East, Nigeria. The soil chemical properties were characterized to fully understand their nature. Metagenomic approach was used to extract soil DNA using the fast DNA Spin Kit extraction technique. The PCR-electrophoresed DNA bands were excised and subjected to a full scale Denaturing Gradient Gel Electrophoresis (DGGE) analysis. DGGE fingerprinting for the PCR-16S rDNA product revealed a diverse profile of complex population of bacterial community in the study area. The study shows that more bacterial community can be fully investigated using molecular techniques rather than traditional culture method. The implication of the results obtained is discussed.

关键词： Metagenomic Fingerprinting Bacterial Community PCR-DGGE Culture Method

<i>In-Situ</i>Biostimulatory Effect of Selected Organic Wastes on Bacterial Atrazine Biodegradation

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Advances in Microbiology 2012年第4期2卷 587-592页

作者： Ahmed Faruk Umar Fatimah Tahir Michael Larkin Olubukola Mojisola Oyawoye Balarabe Lawal Musa Mohammed Bello Yerima ediga bede agbo Biological Sciences Programme Abubakar Tafawa Balewa University Bauchi Nigeria Microbiology Department Usmanu Dan Fodio University Sokoto Nigeria Queens University Environmental and Genomic Research (QUESTOR) Center Queens University of Belfast Belfast Northern Ireland UK

The biostimulatory effect of selected organic wastes on bacterial biodegradation of atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) in three agricultural soils in Bauchi state, Nigeria, was carried out. The soil physico-chemical characteristics were investigated to further understand the environmental conditions of the sampling sites. Enrichment technique was used to isolate the atrazine-degrading strains. Mineralization studies were carried out to determine atrazine biodegradation potentials of strains. Polymerase Chain Reaction (PCR) amplification of total nucleic acid of strains revealed several bacterial species based on nucleotide sequence analyses. Biostimulatory effect of selected organic wastes carried out showed minimal to average extent of biodegradation. The highest mean values, in CFU/mL, increase in biomass was recorded in Pseudomonas sp for both Cow dung 16.76 (42.03%) and Chicken droppings 12.32 (38.46%). However, biostimulatory effect using consortia provided more promising results, with 41.51% and 42.08% in Cow dung and Chicken droppings, biomass increase, respectively, in studies conducted. This proves that competition, survival of inoculums, bioavailability of organic amendments and nature of chemical are important factors affecting bioremediation.

关键词： Biostimulation Biodegradation Bioremediation Mineralization Atrazine

AtzABC Catabolic Gene Probe from Novel Atrazine-Degrading Rhodococcus Strain Isolated from a Nigerian Agricultural Soil

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Advances in Microbiology 2012年第4期2卷 593-597页

作者： Ahmed Faruk Umar Fatimah Tahir Michael J. Larkin Olubukola Mojisola Oyawoye Balarabe Lawal Musa Mohammed Bello Yerima ediga bede agbo Biological Sciences Programme Abubakar Tafawa Balewa University Bauchi Nigeria Microbiology Department Usmanu Dan Fodio University Sokoto Nigeria Queens University Environmental and Genomic Research (QUESTOR) Center Queens University of Belfast Belfast Northern Ireland UK

A batch enrichment technique was used to isolate atrazine-degrading Rhodococcus sp strain from an agricultural land with history of atrazine application in Bauchi state, Northeastern Nigeria. The strain was identified on the basis of physiological, biochemical and 16S r RNA gene sequencing. Growth studies and HPLC analysis showed that the strain has potential of atrazine degradation. An investigation into the catabolic genes Atz ABC, which transform atrazine to cyanuric acid, confirms the chromosomal DNA of strain to harbor BC genes, as compared with the positive control, Rhodococcus jostii RHA1. The strain does not possess the Atz A in all catabolic gene probe carried out. The isolation and characterization of the Rhodococcus sp strain showed that catabolic genes may have evolved from a single origin with widespread global distribution, with possible potential in atrazine bioremediation.

关键词： Enrichment Catabolic Genes Bioremediation Atrazine

Salicylic Acid Alleviates Aluminum Toxicity in Tomato Seedlings (Lycopersicum esculentum Mill.) through Activation of Antioxidant Defense System and Proline Biosynthesis

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Advances in Bioscience and Biotechnology 2014年第9期5卷 777-789页

作者： Varalakshmi Surapu Anjaneyulu ediga Balaji Meriga Department of Biochemistry Sri Venkateswara University Tirupati India

The aim of this work was to evaluate the alleviating efficacy of salicylic acid (100, 250 and 500 μM) against the toxic effects of aluminum (Al) on two tomato cultivars (GOWRI and SIRI) differing in their sensitivity to Al stress. Al treatment (500 μM) caused 40% - 80% drop in plant growth, relative water content (RWC) and cell viability and a reduction of 1 - 2.5 fold and 0.5 - 2 fold in glutathione and proline content respectively, when compared to their control plants grown in Al free medium. Al treatment also resulted in 2 - 5 fold raise in malondialdehyde (MDA) levels, 2 - 3 fold higher Al uptake and 55% - 80% more electrolyte leakage and caused severe DNA damage. Al stress enhanced (1 - 2 fold) the activities of superoxide dismutase (SOD) and peroxidase (POD), but decreased catalase (CAT) activity over their respective controls. Exogenously-applied Salicylic acid (SA) significantly (p < 0.05) and dose-dependently alleviated Al-induced toxicity in tomato seedlings as marked by much improved plant growth, retention of higher RWC, cell viability, glutathione and proline content. SA also caused 0.6 - 1.5 fold reduction in Al uptake, 50% - 80% less electrolyte leakage, 40% - 80% drop in lipid peroxidation and considerable protection against DNA damage. Also, supplementation of SA could considerably reverse the Al-induced changes in the activities of SOD, POD and CAT. Together, our findings demonstrate that, SA is an efficient growth regulator with diversified roles that contribute to its potential alleviating effect against Al induced toxicity and SIRI is a relatively Al-resistant cultivar compared to GOWRI.

关键词： Aluminum Antioxidant Enzymes Proline Malondialdehyde Salicylic Acid