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Evaluation of ligninolytic activity in spent mushroom substrate from four cultivated mushrooms

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Journal of Bioresources and Bioproducts 2022年第4期7卷 288-294页

作者： Ana Marión Pérez-Chávez María Melisa Alberti edgardo Albertó Laboratory of Mycology and Mushroom Cultivation.Instituto Tecnológico de Chascomús(INTECH) Universidad Nacional de San Martín(UNSAM)-Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Intendente Marino AveCC 164.7130ChascomúsBuenos AiresArgentina

Spent mushroom substrate(SMS)is an abundant residue generated by the mushroom industry,which is mostly *** this study,the SMS of four cultivated mushrooms were evaluated as potential sources of ligninolytic *** ostreatus,***,*** and Ganoderma lucidum were cultivated in two different substrates to determine growth,mushroom yields and ligninolytic activity of laccase(Lac)and manganese peroxidase(MnP).Maximum activities in the SMS were 3.731 U/g Lac and 0.191 U/g MnP,both for *** mushroom substrates were also analyzed,and higher activities were found in almost all samples.A correlation test between mushroom yields and ligninolytic activities of each culture was made,and we found that high enzymatic activity in the substrate did not result in high mushroom productivity or vice *** SMS was a good source of ligninolytic *** activity depended on the mushroom species and its affinity to the substrate,and it was not influenced by the mushroom *** of mushrooms in diverse lignocellulosic substrates is gaining interest,and ob-taining ligninolytic enzymes from residual SMS is an excellent alternative in the circular economy concept.

关键词： Laccase Manganese peroxidase Biological efficiency Pleurotus spp Ganoderma lucidum Circular economy

Improving zinc recovery by thermoacidophilic archaeon Acidianus copahuensis using tetrathionate

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Transactions of Nonferrous Metals Society of China 2016年第11期26卷 3004-3014页

作者： Camila CASTRO edgardo R.DONATI CINDEFI (CCT La Plata-CONICET UNLP) Facultad de Ciencias Exactas Universidad Nacional de La Plata (UNLP)

The attachment and bioleaching experiments were conducted to evaluate the zinc recovery from Hualilan ore by thethermoacidophilic archaeon Acidianus copahuensis. Cells of this species pregrown on tetrathionate showed higher capability ofattachment to the ore than cells pregrown on other energy sources and such attachment seemed to be mediated by the product ofextracellular polymeric substances. A. copahuensis achieved a successful bioleaching of the ore reaching 100% of zinc recoverywhen tetrathionate was added. Simultaneous addition of yeast extract and tetrathionate maintained the zinc extraction at higher *** dissolution kinetics was controlled by chemical reaction in cultures with the external addition of tetrathionate but by thediffusion through a product layer of jarosite in the other cultures.

关键词： bioleaching zinc tetrathionate microbial attachment Acidianus copahuensis thermophiles

Perinuclear anti-neutrophil cytoplasmic antibodies (p-anca) in chronic ulcerative colitis: Experience in a Mexican institution

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World Journal of Gastroenterology 2006年第21期12卷 3406-3409页

作者： Jesus K Yamamoto-Furusho Takeshi Takahashi-Monroy Omar Vergara-Fernandez edgardo Reyes Luis Uscanga Departments of Gastroenterology. Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran Mexico City Mexico Departments of Surgery. Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran Mexico City Mexico Departments of Pathology. Institute Nacional de Ciencias Medicas y Nutricion Salvador Zubiran Mexico City Mexico

AIM： To assess the prevalence and clinical value of p-ANCA in a sample of Mexican ulcerative colitis （UC） patients. METHODS： In a prospective, IRB-approved protocol, p-ANCA was determined in 80 patients with UC （mean age, 32 ± 12.9 years）. The severity and extension of disease were determined by clinical methods, searching a statistical association with p-ANCA status. RESULTS： p-ANCA were detected in 41 （51%） patients. Severity of disease was the only clinical variable statistically associated with their presence （P 〈 0.0001; OR = 9; CI 95% = 3.2-24.7）. CONCLUSION： The prevalence of p-ANCA was similar to that reported in other countries. Their presence was associated to UC severity, but offered no more information than the obtained by clinical methods.

关键词： Ulcerative colitis Inflammatory bowel disease Perinuclear anti-neutrophil cytoplasmic antibodies

Epstein-Barr virus-associated gastric carcinoma: Evidence of age-dependence among a Mexican population

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World Journal of Gastroenterology 2005年第39期11卷 6096-6103页

作者： Roberto Herrera-Goepfert Suminori Akiba Chihaya Koriyama Shan Ding edgardo Reyes Tetsuhiko Itoh Yoshie Minakami Yoshito Eizuru Department of Pathology InstitutoNacional de Cancerologia Mexico City Mexico Department of Epidemiology and Preventive Medicine Kagoshima University Graduate School of Medical and Dental Sciences Kagoshima Japan Department of Pathology Instituto Nacional de Ciencias Médicas y de la Nutrición “Salvador Zubirán” Mexico City Mexico Department of Pathology Kagoshima Institute of Preventive Medicine Kagoshima Japan Division of Oncogenic and Persistent Viruses Center for Chronic Viral Diseases Kagoshima University Graduate School of Medical and Dental Sciences Kagoshima Japan

AIM： To investigate features of Epstein-Barr virus （EBV）associated gastric carcinoma （EBVaGC） among a Mexican population. METHODS： Cases of primary gastric adenocarcinoma were retrieved from the files of the Departments of Pathology at the Instituto Nacional de Cancerologia and the Instituto Nacional de la Nutrición in Mexico City. The anatomic site of the gastric neoplasia was identified, and carcinomas were histologically classified as intestinal and diffuse types and subclassified as proposed by the Japanese Research Society for Gastric Cancer. EBV-encoded small non-polyadenylated RNA-1 （EBER-1） in situ hybridization was conducted to determine the presence of EBV in neoplastic cells. RESULTS： We studied 330 consecutive, non-selected, primary gastric carcinomas. Among these, there were 173 male and 157 female patients （male/female ratio 1.1/1）. EBER-1 was detected in 24 （7.3%） cases （male/ female ratio： 1.2/1）. The mean age for the entire group was 58.1 years （range： 20-88 years）, whereas the mean age for patients harboring EBER-1-positive gastric carcinomas was 65.3 years （range： 50-84 years）. Age and histological type showed statistically significant differences, when EBER-1-positive and -negative gastric carcinomas were compared. EBER-1 was detected in hyperplastic- and dysplastic-gastric mucosa surrounding two EBER-1-negative carcinomas, respectively. CONCLUSION： Among Latin-American countries, Mexico has the lowest frequency of EBVaGC. Indeed, the Mexican population 〉50 years of age was selectively affected. Ethnic variations are responsible for the epidemiologic behavior of EBVaGC among the worldwide population.

关键词： Epstein-Barr virus Stomach Lymphoepibheliornalike carcinoma Gastric carcinoma EBV-A EBER-1 LMP-1

Repression of Cell Proliferation by miR319-Regulated TCP4

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Molecular Plant 2014年第10期7卷 1533-1544页

作者： Carla Schommer Juan M. Debernardi edgardo G. Bresso Ramiro E. Rodriguez Javier F. Palatnik IBR (Instituto de Biologia Molecular y Celular de Rosario) CONICET and Facultad de Ciencias Bioquimicas y Farmaceuticas UniversidadNacional de Rosario 2000 Rosario Argentina

Leaf development has been extensively studied on a genetic level. However, little is known about the inter- play between the developmental regulators and the cell cycle machinery--a link that ultimately affects leaf form and size. miR319 is a conserved microRNA that regulates TCP transcription factors involved in multiple developmental pathways, including leaf development and senescence, organ curvature, and hormone biosynthesis and signaling. Here, we analyze the participation of TCP4 in the control of cell proliferation. A small increase in TCP4 activity has an immediate impact on leaf cell number, by significantly reducing cell proliferation. Plants with high TCP4 levels have a strong reduction in the expression of genes known to be active in G2-M phase of the cell cycle. Part of these effects is mediated by induction of miR396, which represses Growth-Regulating Factor （GRF） transcription factors. Detailed analysis revealed TCP4 to be a direct regulator of MIR396b. However, we found that TCP4 can control cell proliferation through additional pathways, and we identified a direct connection between TCP4 and ICK1/KRP1, a gene involved in the progression of the cell cycle. Our results show that TCP4 can activate different pathways that repress cell proliferation.

关键词： microRNA miR396 miR319 TCP GRF ICK1 KRP1 proliferation.

Financial Constraints and Multilevel Perspective： The Case of the Italian Manufacturing Enterprises

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Journal of Modern Accounting and Auditing 2016年第2期12卷 102-110页

作者： edgardo Sica University of Foggia Foggia Italy

Following a multi-level perspective （MLP）, a sustainability transition is the consequence of destabilization pressures from the landscape level that are exerted towards the current unsustainable regime contributing thus to the emergence of niche-level eco-innovations （El）. However, the existence of financial barriers to eco-innovative companies may hinder the development and diffusion of EIs at regime level, jeopardising the creation of windows of opportunity that are necessary for niche-level innovations to succeed. In this framework, the present paper investigates to what extent financial constraints are hampering the eco-innovative investments at regime level, by employing an ad hoc designed survey addressed to a sample of Italian manufacturing enterprises. Results reveal the existence of significant financial barriers to eco-innovative companies which are hindering the development and adoption of incremental technological EIs and organizational EIs at regime level, delaying the transition towards a more sustainable regime. Moreover, findings suggest that environmental reputation of companies can positively contribute to reducing asymmetric information in eco-innovative investments, relieving thus the financial constraints faced by eco-innovative enterprises.

关键词： sustainability eco-innovations (EIs) socio-technical transitions financial constraints

The abductor hallucis muscle flap-a viable surgical solution for treating first ray soft tissue defects

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Plastic and Aesthetic Research 2023年第1期10卷 744-751页

作者： Sitong Chen Timothy P.Cheung edgardo R.Rodriguez-Collazo Department of Surgery Ascension Saint JosephChicagoIL 60657USA Department of Podiatric Surgery Yale New Haven HospitalNew HavenCT 06511USA

Long-standing wounds are at high risk for ***,it is critical to achieve wound healing in a timely manner;however,some complex wounds remain recalcitrant and difficult to *** muscle flaps are an underutilized technique with great utility in the reconstruction of complex foot *** a healthy bleeding base that promotes wound healing,these muscle flaps can greatly benefit the patient and prevent *** this present report,we demonstrate the use of the abductor hallucis muscle flap in the reconstruction of a complex wound following bunion surgery.

关键词： Muscle flap abductor hallucis forefoot wound limb salvage flap reconstruction soft tissue coverage

Estimating the geographic range of a threatened shark in a data-poor region： Cetorhinus maximus in the South Atlantic Ocean

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Current Zoology 2015年第5期61卷 811-826页

作者： Luis O. LUCIFORA Santiago A. BARBINI edgardo E. DI GIACOMO Juan A. WAESSLE Daniel E. FIGUEROA Instituto de Biologia Subtropical - Iguazu Consejo Nacional de Investigaciones Cientificas y Tecnicas Universidad Nacional de Misiones Puerto Iguazu Misiones Argentina Laboratorio de Ictiologia Instituto de Investigaciones Marinas y Costeras Consejo Nacional de Investigaciones Cientificas y Tecnicas Universidad Nacional de Mar del Plata Mar del Plata Provincia de Buenos Aires Argentina Grupo Condros Instituto de Biologia Marina y Pesquera "Almirante Stomi" Universidad Nacional del Comahue San Antonio Oeste Rio Negro Argentina Instituto Nacional de Investigacion y Desarrollo Pesquero Mar del Plata Provincia de Buenos Aires Argentina

The distribution of the planktivorous basking shark Cetorhinus maximus is influenced by zooplankton abundance at small scales and temperature at medium scales in the North Atlantic. Here, we estimate the distribution of basking sharks on South Atlantic continental shelves, and the relative importance of chlorophyll concentration, as a proxy for zooplankton abun- dance, and temperature in determining habitat suitability for basking sharks at large scales. We used maximum entropy （MaxEnt） and maximum likelihood （MaxLike） species distribution modelling to test three hypotheses： the distribution of basking sharks is determined by （1） temperature, （2） chlorophyll concentration, or （3） both chlorophyll and temperature, while considering other factors, such as oxygen and salinity. Off South America, basking shark habitat included subtropical, temperate and cool-temperate waters between approximately 20°S and 55°S. Off Africa, basking shark habitat was limited to cool-temperate waters off Namibia and southern South Africa. MaxLike models bad a better fit than MaxEnt models. The best model included minimum chlorophyll concentration, dissolved oxygen concentration, and sea surface temperature range, supporting hypothesis 3. However, of all variables included in the best model, minimum chlorophyll concentration had the highest influence on basking shark distribution. Unlike the North Atlantic distribution, the South Atlantic distribution of basking sharks includes subtropical and cool-temperate waters. This difference is explained by high minimum chlorophyll concentration off southern Brazil as compared to North Atlantic subtropical areas. Observations in other regions of the world support this conclusion. The highest habitat suitability for basking sharks is located close to nearshore areas that experience high anthropogenic impact [Current Zoology 61 （5）： 811-826, 2015].

关键词： Basking shark Chondrichthyes Geographic range MaxEnt MaxLike Southern Hemisphere

Randomized intervention to assess the effectiveness of an educational video on organ donation intent among Hispanics in the New York metropolitan area

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World Journal of Transplantation 2023年第4期13卷 190-200页

作者： Renee Pekmezaris edgardo Cigaran Vidhi Patel Damian Clement Christine L Sardo Molmenti Ernesto Molmenti Feinstein Institutes for Medical Research Northwell HealthManhassetNY 11030United States Department of Nephrology Northwell Health/Zucker School of Medicine at HosftraManhassetNY 11030United States

BACKGROUND The Hispanic community has a high demand for organ donation but a shortage of *** investigating factors that could promote or hinder organ donation have examined emotional video *** acting as barriers to organ donation registration have been classified as:(1)Bodily integrity;(2)medical mistrust;(3)“ick”-feelings of disgust towards organ donation;and(4)“jinx”-fear that registration may result in one dying due to premeditated *** predict that by providing necessary information and education about the donation process via a short video,individuals will be more willing to register as organ *** To determine perceptions and attitudes regarding barriers and facilitators to organ donation intention among Hispanic residents in the New York metropolitan *** This study was approved by the Institutional Review Board at Northwell *** approval reference number is No.19-0009(as presented in Supplementary material).Eligible participants included Hispanic New York City(NYC)residents,18 years of age and above,who were recruited voluntarily through Cloud Research and participated in a larger randomized survey study of NYC *** survey an 85-item Redcap survey measured participant demographics,attitudes,and knowledge of organ donation as well as the intention to register as an organ *** checks were implemented throughout the survey,and responses were excluded for those who did *** were randomly assigned two-between subject conditions:To view a short video on organ donation and then proceed to complete the survey(i.e.,video first)and view the same video at the end of the survey(video last).No intra-group activities were *** study utilized an evidenced-based emotive educational intervention(video)which was previously utilized and was shown to increase organ donation registration rates at the Ohio Department of Motor *** were analyzed using Jamovi statistical softw

关键词： Community engagement and health Health equity Diversity and inclusion Health policy Kidney donation Minority health and disparities Organ transplant