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Taxotere resistance in SUIT Taxotere resistance in pancreatic carcinoma cell line SUIT 2 and its sublines

World Journal of Gastroenterology 2001年第6期7卷 855-859页

作者： edgar Staren Takeshi lwamura HubertAppert JohnHoward Department of Surgery Medical College of OhioToledoOhioUSA Department of Surgery Miyazaki Medical CollegeMiyazakiJapan

AIM: To investigate the specific mechanisms of intrinsic and acquired resistance to taxotere (TXT) in pancreatic adenocarcinoma (PAC). METHODS: MTT assay was used to detect the sensitivity of PAC cell line SUIT-2 and its sublines (S-007, S-013, S-020, S-028 and TXT selected SUIT-2 cell line, S2/TXT) to TXT. Mdr1 (P-gp), multidrug resistance associated protein (MRP), lung resistance protein (LRP) and beta-tubulin isotype gene expressions were detected by RT-PCR. The functionality of P-gp and MRP was tested using their specific blocker verapamil (Ver) and indomethacin (IMC), respectively. The transporter activity of P-gp was also confirmed by Rhodamine 123 accumulation assay. RESULTS: S-020 and S2/TXT were found to be significantly resistant to TXT(19 and 9.5-fold to their parental cell line SUIT-2, respectively). RT-PCR demonstrated strong expression of Mdr1 in these two cell lines, but weaker expression or no expression in other cells lines. MRP and LRP expressions were found in most of these cell lines. The TXT-resistance in S2-020 and S2/TXT could be reversed almost completely by Ver, but not by IMC. Flow cytometry showed that Ver increased the accumulation of Rhodamine-123 in these two cell lines. Compared with S-020 and SUIT-2, the levels of beta-tubulin isotype II, III expressions in S-2/TXT were increased remarkably. CONCLUSION: The both intrinsic and acquired TXT-related drug resistance in these PAC cell lines is mainly mediated by P-gp, but had no relationship to MRP and LRP expressions. The increases of beta-tubulin isotype II, III might be collateral changes that occur when the SUIT-2 cells are treated with TXT.

关键词： Drug Resistance, Neoplasm Taxoids Antineoplastic Agents, Phytogenic Carcinoma Humans Paclitaxel derivatives Pancreatic Neoplasms Research Support, Non-U.S. Gov't Tumor Cells, Cultured

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欧洲学校里的科学教育

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全球教育展望 2002年第2期31卷 20-21页

作者： edgar Jenkins 科学教育政策教授

在新千年开始之际,可以了解到的有关欧洲学校科学教育的情况比过去任何时候都要多.除了各国和国际一级的出版物(如,教科文组织的和经合发组织的)提供的资料外,还可以利用其它一些调查研究和比较研究的报告.

关键词：欧洲学校科学教育高等教育教育水平

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The Resolution of the Great 20th Century Debate in the Foundations of Mathematics

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Advances in Pure Mathematics 2016年第3期6卷 144-158页

作者： edgar E. Escultura GVP-Professor V. Lakshmikantham Institute for Advanced Studies GVP College of Engineering JNT University Kakinada Madurawada Visakhapatnam India

The paper resolves the great debate of the 20th century between the three philosophies of mathematics-logicism, intuitionism and formalism—founded by Bertrand Russell and A. N. Whitehead, L. E. J. Brouwer and David Hilbert, respectively. The issue: which one provides firm foundations for mathematics? None of them won the debate. We make a critique of each, consolidate their contributions, rectify their weakness and add our own to resolve the debate. The resolution forms the new foundations of mathematics. Then we apply the new foundations to assess the status of Hilbert’s 23 problems most of which in foundations and find out which ones have been solved, which ones have flawed solutions that we rectify and which ones are open problems. Problem 6 of Hilbert’s problems—Can physics be axiomatized?—is answered yes in E. E. Escultura, Nonlinear Analysis, A-Series: 69(2008), which provides the solution, namely, the grand unified theory (GUT). We also point to the resolution of the 379-year-old Fermat’s conjecture (popularly known as Fermat’s last theorem) in E. E. Escultura, Exact Solutions of Fermat’s Equations (Definitive Resolution of Fermat’s Last Theorem), Nonlinear Studies, 5(2), (1998). Likewise, the proof of the 274-year-old Goldbach’s conjecture is in E. E. Escultura, The New Mathematics and Physics, Applied Mathematics and Computation, 138(1), 2003.

关键词： Axiom of Choice Banach-Tarski Paradox Goldbach’s Conjecture Logicism Constructivism Fermat’s Conjecture Field Axioms Formalism Qualitative Modelling Rational Thought Self-Reference

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Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma (Meccus) pallidipennis, under different feeding conditions

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Insect Science 2019年第5期26卷 796-808页

作者： Ana E.Gutierrez-Cabrera Wesley F.Zandberg edgar Zenteno Mario H.Rodriguez Bertha Espinoza Carl Lowenberger CONACyT-Centro de Investigacion Sobre Enfermedades Infecciosas Instituto Nacional de Salud PublicaCuernavacaMorelosMexico Department of Chemistry Simon Fraser UniversityBurnabyBCCanada Department of Chemistry The University of British Columbia OkanaganKelownaBCCanada Facultad de Medicina Departamento de BioquimicaUniversidad Nacional Autdmoma de Mexico Mexico CityDFMexico Centro de Investigacion Sobre Enfermedades Infecciosas Instituto Nacional de Salud PublicaCuernavaca MorelosMexico Departamento de Inmunologia Instituto de Investigaciones BiomedicasUniversidad Nacional Autonoma de Mexico Ciudad UniversitariaMexico CityDFMexico Department of Biological Sciences Simon Fraser UniversityBurnabyBCCanada

Trypanosoma cruzi,the causative agent of Chagas disease, interacts with molecules in the midgut of its insect vector to multiply and reach the infective stage. Many studies suggest that the parasite binds to midgut-specific glycans. We identified several glycoproteins expressed in the intestine and perimicrovillar membrane (PMM) of Triatoma (Meccus) pallidipennis under different feeding conditions. In order to assess changes in protein-linked glycans, we performed lectin and immunoblot analyses on glycoprotein extracts from these intestinal tissues using well-characterized lectins, and an antibody, which collectively recognize a wide range of different glycans epitopes. We observed that the amount and composition of proteins and glycoproteins associated with different glycans structures changed over time in the intestines and PMM under different physiological conditions. PMM extracts contained a wide variety of glycoproteins with different sugar residues, including abundant high-mannose and complex sialylated glycans. We propose that these molecules could be involved in the process of parasite-vector interactions.

关键词： feeding conditions glycoproteins lectins perimicrovillar membrane triatomine vector-parasite interactions

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New injectable two-step forming hydrogel for delivery of bioactive substances in tissue regeneration

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Regenerative Biomaterials 2019年第3期6卷 149-162页

作者： edgar Pe´rez-Herrero Patricia Garcı´a-Garcı´a Jaime Go´mez-Morales Matias Llabre´s Araceli Delgado Carmen E´vora Department of Chemical Engineering and Pharmaceutical Technology University of La LagunaLa Laguna 38200TenerifeSpain Institute of Biomedical Technologies(ITB) Center for Biomedical Research of the Canary Islands(CIBICAN)University of La LagunaLa Laguna 38071TenerifeSpain Laboratory of Crystallographic Studies Andalusian Earth Sciences InstituteSpanish Research Council—University of GranadaArmillaGranada 18100Spain Institute of Tropical Diseases and Healthcare of the Canary Islands University of La LagunaLa Laguna 38203TenerifeSpain

A hydrogel based on chitosan,collagen,hydroxypropyl-c-cyclodextrin and polyethylene glycol was developed and *** incorporation of nano-hydroxyapatite and pre-encapsulated hydrophobic/hydrophilic model drugs diminished the porosity of hydrogel from 81.6262.25%to 69.9863.07%.Interactions between components of hydrogel,demonstrated by FTIR spectroscopy and rheology,generated a network that was able to trap bioactive components and delay the burst *** thixotropic behavior of hydrogel provided adaptability to facilitate its implantation in a minimally invasive *** profiles from microspheres included or not in hydrogel revealed a two-phase behavior with a burst-and a controlled-release *** same release rate for microspheres included or not in the hydrogel in the controlled-release period demonstrated that mass transfer process was controlled by internal *** diffusion coefficients,Deff,that describe internal diffusion inside microspheres,and mass transfer coefficients,h,*** contribution of hydrogel to mass transfer,were determined using‘genetic algorithms’,obtaining values between 2.64·10^(-15) and 6.67·10^(-15)m^(2)/s for Deff and 8.50·10^(-10) to 3.04·10^(-9) m/s for *** proposed model fits experimental data,obtaining an R2-value ranged between 95.41 and 98.87%.In vitro culture of mesenchymal stem cells in hydrogel showed no manifestations of intolerance or toxicity,observing an intense proliferation of the cells after 7 days,being most of the scaffold surface occupied by living cells.

关键词： hydrogel collagen–cyclodextrin–chitosan rheology mass transfer estradiol FITC-dextran

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A planar defect spin sensor in a two-dimensional material susceptible to strain and electric fields

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npj Computational Materials 2023年第1期9卷 775-782页

作者： Péter Udvarhelyi Tristan Clua-Provost Alrik Durand Jiahan Li James H.edgar Bernard Gil Guillaume Cassabois Vincent Jacques Adam Gali Wigner Research Centre for Physics P.O.Box 49H-1525 BudapestHungary Budapest University of Technology and Economics Institute of PhysicsDepartment of Atomic PhysicsMűegyetem rakpart 3.1111 BudapestHungary Laboratoire Charles Coulomb Universitéde Montpellier and CNRS34095 MontpellierFrance Tim Taylor Department of Chemical Engineering Kansas State UniversityManhattanKS 66506USA

The boron-vacancy spin defect(V_(B)^(-))in hexagonal boron nitride(hBN)has a great potential as a quantum sensor in a two-dimensional material that can directly probe various external perturbations in atomic-scale proximity to the quantum sensing ***,we apply first-principles calculations to determine the coupling of the VB electronic spin to strain and electric *** work unravels the interplay between local piezoelectric and elastic effects contributing to the final response to the electric *** theoretical predictions are then used to analyse optically detected magnetic resonance(ODMR)spectra recorded on hBN crystals containing different densities of V_(B)^(-) *** prove that the orthorhombic zero-field splitting parameter results from local electric fields produced by surrounding charge *** work paves the way towards applications of V_(B)^(-) centres for quantitative electric field imaging and quantum sensing under pressure.

关键词： defect quantum boron

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Cell-mediated and cell membrane-coated nanoparticles for drug delivery and cancer therapy

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Cancer Drug Resistance 2020年第4期3卷 879-911页

作者： Serkan Yaman Uday Chintapula edgar Rodriguez Harish Ramachandramoorthy Kytai T.Nguyen Department of Bioengineering University of Texas at ArlingtonArlingtonTX 76010USA Joint Bioengineering Program University of Texas Southwestern Medical CenterDallasTX 75235USA

Nanotechnology-based drug delivery platforms have been developed over the last two decades because of their favorable features in terms of improved drug bioavailability and *** recent advancement in nanotechnology platforms,this approach still falls short to meet the complexity of biological systems and diseases,such as avoiding systemic side effects,manipulating biological interactions and overcoming drug resistance,which hinders the therapeutic outcomes of the NP-based drug delivery *** address these issues,various strategies have been developed including the use of engineered cells and/or cell membrane-coated *** membrane receptor profiles and characteristics are vital in performing therapeutic functions,targeting,and homing of either engineered cells or cell membrane-coated nanocarriers to the sites of *** this context,we comprehensively discuss various cell-and cell membrane-based drug delivery approaches towards cancer therapy,the therapeutic potential of these strategies,and the limitations associated with engineered cells as drug carriers and cell membrane-associated drug ***,we review various cell types and cell membrane receptors for their potential in targeting,immunomodulation and overcoming drug resistance in cancer.

关键词： Cell membrane-based drug delivery cell-mediated drug delivery membrane receptors drug carriers cancer drug resistance nanoparticles

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The Logic and Fundamental Concepts of the Grand Unified Theory

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Journal of Modern Physics 2013年第8期4卷 213-222页

作者： edgar E. Escultura Prof. V. Laksshmikantham Institute for Advanced Studies GVP College of Engineering Jawaharlal Nehru Technical University Kakinada Vishakapatnam India

The grand unified theory (GUT) originated in mathematics with this question: why are there long standing unsolved problems in mathematics, e.g., Fermat’s conjecture (also known as Fermat’s last theorem (FLT))? The answer came quickly: its underlying fields—foundations and the real number system—are defective. In particular, formal logic is inapplicable to mathematics (language of science) and the real number system is inconsistent. Critique-rectification of these fields was undertaken leading to a new mathematical methodology and the consistent new real number system that provides the main mathematics of GUT. Similar question was posed in physics: why are there long standing problems, e.g., the gravitational n-body and turbulence problems? The answer: the present methodology, quantitative modeling is inadequate and the remedy is a new methodology—qualitative mathematics and modeling that solved these problems and provided the initial formulation of GUT. This paper presents the basic logic of GUT and its fundamental concepts, particularly, the superstring or fundamental building block of matter.

关键词： Anti-Matter Big Bang Black Hole Chaos Cosmic Burst Cosmological Body Electromagnetic Wave Generalized Physical Fractal Qualitative Modeling Quantum and Macro Gravity Quark Primum Superstring Turbulence

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索尼VRD-MC1 DVD直连式多功能刻录机

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微型计算机 2006年第26期26卷 118-119页

作者： edgar

一贯倡导科技连接生活的索尼，这次推出的VRD-MC1几乎完全打破了刻录机在我心目中的传统印象。无需PCVRD-MC1直接将传统IT产品与家电紧密地结合在了一起。

关键词：刻录机索尼VRD-MC1 多功能 DVD

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Electromagnetic Treatment of Genetic Diseases

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Journal of Biomaterials and Nanobiotechnology 2012年第2期3卷 292-300页

作者： edgar E. Escultura Professor V. Lakshmikantham – GVP Institute for Advanced Studies GVP College of Engineering JNT University AP India

The paper offers an overview of quantum and macro gravity, two of the three pillars of the Grand Unified Theory (GUT), the other thermodynamics, developed in a series of papers since the solution of the gravitational n-body problem in 1997 (J. Nonlinear Analysis, A-Series: Theory, Methods and Applications, Vol. 30, No. 8, 1997, pp. 5021 - 5032) and consolidated in the paper, The Grand Unified Theory (J. Nonlinear Analysis, A-Series: Theory: Method and Applications, Vol. 69, No. 3, 2008, pp. 823 - 831). GUT is further advanced by the paper, The Mathematics of GUT (J. Nonlinear Analysis, A-Series: Theory: Method and Applications, Vol. 71, 2009, pp. e420 - e431) and the discovery of more natural laws in the course of analyzing and explaining the disastrous final flight of the Columbia Space Shuttle in 2004 (J. Nonlinear Studies, Vol. 14, No. 3, 2007, pp. 241 - 260). Qualitative modeling was the key to the development of GUT and its theoretical and practical applications. The relevant natural laws of GUT that provide the foundations of the Unified Theory of Evolution are stated. GUT provides the basis for the development of the electromagnetic engine and the Unified Theory of Evolution, its theoretical application, for the development of appropriate technology for electromagnetic treatment of genetic diseases such as cancer, systemic lupos erythematosus, diabetes, muscular dystrophy and mental disorder, the central focus of this paper.

关键词： Fractal Gene Chaos Primum Superstring Turbulence Brain Wave Composite Gene Dark Matter Electromagnetic Wave Qualitative Modeling Brain Wave Superposition Genetic Alteration Encoding Modification Sterilization

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