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Heficobacter pylori Biofilm Formation and Gene expression on Abiotic Surfaces Using a Cyanobacterial extract

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Journal of Life Sciences 2012年第12期6卷 1317-1327页

作者： Alba e. Vega Fabio A. Persia Gabriel A. Salinas-Ibanez Teresa Alarcon Stella M.C. Lucero estrada Teresa I.Cortinas Humberto J. Silva Microbiology Laboratory Department of Biochemistry and Biological Sciences Faculty of Chemistry Biochemistry andPharmacy National University of San Luis San Luis 5700 Argentina Department of Microbiology University Hospital La Princesa Princess Health Research Institute (IP) Madrid 28006 Spain

The effects of a cyanobacterial extract （Ce） on Helicobacter pylori biofilm formation onto hydrophobic and hydrophilic abiotic surfaces and the expression of luxS, flaA, omp18, lpxD and ureA genes associated to biofilm were studied. NCTC11638 reference strain and HP796, a resistant clinical isolate, were grown in mueller-Hinton broth supplemented with 5% fetal calf serum （FCS） or 1% Ce. The ability to form biofilm, viability, morphological changes and gene expression of adhered H. pylori cells were determined. The strains were able to form biofilm on both surfaces with the nutritional supplements analyzed. H. pylori conserved a characteristic bacillary morphology and viability with Ce. Cells attachment was higher with Ce than FCS regardless of strains and surfaces. The most remarkable increase in gene expression was observed with the ompl8 gene using the Ce supplement, indicating the important participation of outer membrane proteins in biofilm establishment. The clinical isolate showed similar and even greater gene expression than the reference strain. The results obtained indicated that the nutrients provided by Ce favored biofilm formation with retained pathogenicity that under certain conditions can occur in natural aquatic environments.

关键词： Helicobacterpylori biofilm cyanobacterial extract gene expression.

目标粗糙表面偏振二向反射分布函数实验研究方法改进

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飞行器测控学报 2017年第5期36卷 365-374页

作者：刘卿战永红杨迪王雅萍曾嫦娥北京跟踪与通信技术研究所北京100094

由于利用传统pBRDF(polarimetric Bidirectional Reflectance Directional Function,偏振二向反射分布函数)的测试方法效率较低,精度不高,难以满足研究需要,因此基于自主研发制造的二维转台系统,设计了散射光Stokes参量及pBRDF矩阵的新... 详细信息

由于利用传统pBRDF(polarimetric Bidirectional Reflectance Directional Function,偏振二向反射分布函数)的测试方法效率较低,精度不高,难以满足研究需要,因此基于自主研发制造的二维转台系统,设计了散射光Stokes参量及pBRDF矩阵的新的测试方法,以提高测试效率和精度。该方法将单点测试次数由传统方法的16次降低到4次,测试效率更高,根据Muller矩阵的对称性进行数据处理,反演精度更高。设计了实验验证方法。验证实验的实验对象包括标准反射板、绿色功能涂层。实验结果验证了所提测试方法的准确性及有效性,为今后目标粗糙表面pBRDF模型的改进提供了高精度的实验支撑。此外,分析实验数据可知,反射光的强度曲线与偏振分量曲线区别较大,因此使用偏振成像探测技术可有效提高目标探测和识别的能力。

关键词：目标特性偏振二向反射分布函数(pBRDF) 实验方法改进比较测量法

Notes,outline and divergence times of Basidiomycota

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Fungal Diversity 2019年第6期99卷 105-367页

作者： Mao-Qiang He Rui-Lin Zhao Kevin D.Hyde Dominik Begerow Martin Kemler Andrey Yurkov eric H.C.McKenzie Olivier Raspe Makoto Kakishima Santiago Sanchez-Ramırez else C.Vellinga Roy Halling Viktor Papp Ivan V.Zmitrovich Bart Buyck Damien ertz Nalin N.Wijayawardene Bao-Kai Cui Nathan Schoutteten Xin-Zhan Liu Tai-Hui Li Yi-Jian Yao Xin-Yu Zhu An-Qi Liu Guo-Jie Li Ming-Zhe Zhang Zhi-Lin Ling Bin Cao Vladimir Antonin Teun Boekhout Bianca Denise Barbosa da Silva eske De Crop Cony Decock Balint Dima Arun Kumar Dutta Jack W.Fell Jozsef Geml Masoomeh Ghobad-Nejhad Admir J.Giachini Tatiana B.Gibertoni Sergio P.Gorjon Danny Haelewaters Shuang-Hui He Brendan P.Hodkinson egon Horak Tamotsu Hoshino Alfredo Justo Young Woon Lim Nelson Menolli Jr Armin Mesic Jean-Marc Moncalvo Gregory M.mueller La szlo G.Nagy RHenrik Nilsson Machiel Noordeloos Jorinde Nuytinck Takamichi Orihara Cheewangkoon Ratchadawan Mario Rajchenberg Alexandre G.S.Silva-Filho Marcelo Aloisio Sulzbacher Zdenko Tkalcec Ricardo Valenzuela Annemieke Verbeken Alfredo Vizzini Felipe Wartchow Tie-Zheng Wei Michael WeiB Chang-Lin Zhao Paul M.Kirk State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101People’s Republic of China Department of entomology and Plant Pathology Faculty of AgricultureChiang Mai UniversityChiang Mai 50200Thailand Center of excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand College of Life Sciences University of Chinese Academy of SciencesHuairou DistrictBeijing 100408People’s Republic of China Ruhr-Universitat Bochum AG GeobotanikND03UniversitatsstraBe 15044801 BochumGermany Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures InhoffenstraBe 7B38124 BrunswickGermany Manaaki Whenua-Landcare Research Private Bag 92170AucklandNew Zealand Research Department Meise Botanic GardenNieuwelaan 381860 MeiseBelgium Service General de l'enseignement superieur et de la Recherche scientifique Federation Wallonie-BruxellesLavallee 11080 BrusselsBelgium University of Tsukuba TsukubaIbaraki 305-8572Japan Department of ecology and evolutionary Biology University of Toronto25 Willcocks St.TorontoON M5S 3B2Canada Berkeley USA Institute of Systematic Botany The New York Botanical GardenBronxNY 10458-5126USA Department of Botany Szent Istvan University1518 BudapestHungary Komarov Botanical Institute Russian Academy of SciencesProfessor Popov st.2Saint PetersburgRussia 197376 Institut pour Systematique ecologie Biodiversite(ISYeB UMR 7205) Sorbonne universiteMuseum National d’Histoire Naturelle CP 39CNRSDepartement Origines et Evolution12 Rue Buffon75005 ParisFrance Institute of Microbiology Beijing Forestry UniversityBeijing 100083People’s Republic of China Department of Biology Research Group MycologyGhent UniversityK.L.Ledeganckstraat 359000 GhentBelgium State Key Laboratory of Applied Microbiology Southern China Guangdong Provincial Key Laboratory of Microbial Culture Collection and ApplicationGuangdong Institute of MicrobiologyGuangzhou 510070People’s Republic of China Department of Botany Moravian Mu

The Basidiomycota constitutes a major phylum of the kingdom Fungi and is second in species numbers to the *** present work provides an overview of all validly published,currently used basidiomycete genera to date in a single *** outline of all genera of Basidiomycota is provided,which includes 1928 currently used genera names,with 1263 synonyms,which are distributed in 241 families,68 orders,18 classes and four *** provide brief notes for each accepted genus including information on classification,number of accepted species,type species,life mode,habitat,distribution,and sequence ***,three phylogenetic analyses with combined LSU,SSU,5.8s,rpb1,rpb2,and ef1 datasets for the subphyla Agaricomycotina,Pucciniomycotina and Ustilaginomycotina are conducted,*** time estimates are provided to the family level with 632 species from 62 orders,168 families and 605 *** study indicates that the divergence times of the subphyla in Basidiomycota are 406-430 Mya,classes are 211-383 Mya,and orders are 99-323 Mya,which are largely consistent with previous *** this study,all phylogenetically supported families were dated,with the families of Agaricomycotina diverging from 27-178 Mya,Pucciniomycotina from 85-222 Mya,and Ustilaginomycotina from 79-177 *** times as additional criterion in ranking provide additional evidence to resolve taxonomic problems in the Basidiomycota taxonomic system,and also provide a better understanding of their phylogeny and evolution.

关键词： Classification Molecular clock Fungi Systematics Taxonomy

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照护艾滋病患者的感染科护士工作满意度调查

实用医院临床杂志 2014年第5期11卷 158-160页

作者：刘艳花袁素娥中南大学湘雅医院感染病科湖南长沙410008

目的调查照护艾滋病患者的感染科护士的工作满意度,并分析相关影响因素。方法本文以某地区4所三甲医院照护艾滋病患者的感染科护士为研究对象,采用护士工作满意度量表测量感染科护士的工作满意程度,并分析相关影响因素。结果照护艾滋病... 详细信息

目的调查照护艾滋病患者的感染科护士的工作满意度,并分析相关影响因素。方法本文以某地区4所三甲医院照护艾滋病患者的感染科护士为研究对象,采用护士工作满意度量表测量感染科护士的工作满意程度,并分析相关影响因素。结果照护艾滋病患者的感染科护士工作总体满意度(59.2±8.5)分。Logistic回归分析结果显示学历水平、收入状况、工作时间是影响感染科护士工作满意度的因素。结论护理管理者需要提升感染科护士的内外因素,有助于改善工作满意度,从而进一步提升护理服务质量及患者满意度。

关键词：临床护理艾滋病工作满意度现况调查

吸气对抗阻力或标准化的穆勒操纵:一项提高肺动脉对比度的新型CT呼吸技术初步研究

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国际医学放射学杂志 2016年第1期 83-84页

作者： A.Gutzeit J.M.Froehlich S.Walti J.e.Roos M.Meissnitzer K.Hergan 余鸿鸣 Department of Radiology Paracelsus Medical University Salzburg

摘要目的前瞻性比较最近推出的吸气对抗阻力呼吸方法与标准呼吸方法,分析前者能否得到更高的肺动脉CT对比度。材料与方法研究经医学伦理委员会批准,所有项目均需书面知情同意。15例没有可疑肺栓子的病人被随机分配到4个不同的组,在常规C... 详细信息

摘要目的前瞻性比较最近推出的吸气对抗阻力呼吸方法与标准呼吸方法,分析前者能否得到更高的肺动脉CT对比度。材料与方法研究经医学伦理委员会批准,所有项目均需书面知情同意。15例没有可疑肺栓子的病人被随机分配到4个不同的组,在常规CT检查中有不同的呼吸动作(对抗阻力吸气,Valsalva吸气、呼气)。对比增强在中央和周边部分的肺动脉进行测定和比较。结果外周肺动脉增强在吸气相增加平均密度是138.14 HU(P=0.001),而在Valsalva平均

关键词：肺动脉平均密度 CT

Improved measurement of the reactor antineutrino flux and spectrum at Daya Bay

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Chinese Physics C 2017年第1期41卷 11-46页

作者：安丰鹏 A.B.Balantekin H.R.Band M.Bishai S.Blyth 曹德文曹国富曹俊岑吴镕陳日朗常劲帆張立楚张昀陈和生陈泉佑陈少敏陈义学陈羽程佳樺程捷程雅苹成兆侃 J.J.Cherwinka 朱明中 A.Chukanov J.P.Cummings J.de Arcos 邓子艳丁雪峰丁雅韵 M.V.Diwan M.Dolgareva J.Dove D.A.Dwyer W.R.edwards R.Gill M.Gonchar 龚光华宫辉 M.Grassi 顾文强关梦云郭磊郭如盼郭新恒郭子溢 R.W.Hackenburg 韩然 S.Hans 何苗 K.M.Heeger 衡月昆 A.Higuera Y.K.Hor 熊怡胡貝禎胡涛胡维黄恩泉黄翰雄黄性涛 P.Huber 霍文驹 G.Hussain D.e.Jaffe P.Jaffke 任國綸 S.Jetter 季向盼季筱璐焦健斌 R.A.Johnson D.Jones J.Joshi 康丽 S.H.Kettell S.Kohn M.Kramer 關健强郭文伟郭天能 T.J.Langford K.Lau L.Lebanowski J.Lee 李曉菁雷瑞庭 R.Leitner 李超李登杰李飞李高嵩李秋菊黎山峰 S.C.Li 李卫东李小男李玉峰李志兵梁昊林政儒林贵林林盛鑫林士凱林禹丞凌家杰 J.M.Link L.Littenberg B.R.Littlejohn 刘大为刘江来刘金昌 C.W.Loh 陆昌国路浩奇陆稼书陆锦标吕志鹏马秋梅马晓妍马续波马宇倩 Y.Malyshkin D.A.Martinez Caicedo K.T.McDonald R.D.McKeown I.Mitchell M.Mooney Y.Nakajima J.Napolitano D.Naumov e.Naumova 倪浩然宁哲 J.P.Ochoa-Ricoux A.Olshevskiy 潘孝儒 J.Park S.Patton V.Pec 彭仁杰 L.Pinsky 潘振声齐法制祁鸣钱鑫 N.Raper 任杰 R.Rosero B.Roskovec 阮锡超 H.Steiner 孙功星孙吉良唐炜 D.Taychenachev K.TVeskov K.V.Tsang C.e.Tull N.Viaux B.Viren V.Vorobei 王正祥王萌王乃彦王瑞光王为王玺王贻芳王喆王铮王志民魏瀚宇温良剑 K.Whisnant C.G.White L.Whitehead T.Wise 黄显诺黄振辉 e.Worcester 吳珈豪吴群吴文杰夏冬梅夏经凯邢志忠徐建一徐吉磊徐晔薛涛杨长根杨海波杨雷阳马生杨梦婷叶梅 Z.Ye 叶铭芳杨炳麟于泽源曾珊占亮张超张宏浩张家文张清民张玄同张一鸣张一心张玉美张志坚张智勇张子平赵洁赵庆旺赵豫斌钟玮丽周莉 N.Zhou 庄红林邹佳恒 Institute of Modern Physics East China University of Science and Technology University of Wisconsin Department of Physics Yale University Brookhaven National Laboratory Department of Physics National Taiwan University National United University Nanjing University Institute of High energy Physics Chinese University of Hong Kong Institute of Physics National Chiao-Tung University Shandong University Department of engineering Physics Tsinghua University North China electric Power University Shenzhen University Sun Yat-Sen (Zhongshan) University Joint Institute for Nuclear Research Siena College Department of Physics Illinois Institute of Technology Department of Physics University of Illinois at Urbana-Champaign Lawrence Berkeley National Laboratory Department of Physics and Astronomy Shanghai Jiao Tong University Shanghai Laboratory for Particle Physics and Cosmology Beijing Normal University Department of Chemistry and Chemical Technology Bronx Community College Department of Physics University of Houston Center for Neutrino Physics Virginia Tech China Institute of Atomic energy University of Science and Technology of China School of Physics Nankai University Department of Physics University of Cincinnati Department of Physics College of Science and Technology Temple University Dongguan University of Technology Department of Physics University of California Department of Physics The University of Hong Kong Charles University Faculty of Mathematics and Physics Joseph Henry Laboratories Princeton University Department of Nuclear Science and Technology School of Energy and Power Engineering Xi’an Jiaotong University Instituto de F'?sica Pontificia Universidad Católica de Chile California Institute of Technology College of William and Mary Department of Physics Applied Physics and Astronomy Rensselaer Polytechnic Institute China General Nuclear Power Group College of electronic Science and engineering National University of Defense Technology Iowa State University Chongqing University

A new measurement of the reactor antineutrino flux and energy spectrum by the Daya Bay reactor neutrino experiment is reported. The antineutrinos were generated by six 2.9 GWth nuclear reactors and detected by eight antineutrino detectors deployed in two near（560 m and 600 m flux-weighted baselines） and one far（1640 m flux-weighted baseline） underground experimental halls. With 621 days of data, more than 1.2 million inverse beta decay（IBD） candidates were detected. The IBD yield in the eight detectors was measured, and the ratio of measured to predicted flux was found to be 0.946±0.020（0.992±0.021） for the Huber＋mueller（ILL＋Vogel） model. A 2.9σ deviation was found in the measured IBD positron energy spectrum compared to the predictions. In particular, an excess of events in the region of 4–6 MeV was found in the measured spectrum, with a local significance of 4.4σ. A reactor antineutrino spectrum weighted by the IBD cross section is extracted for model-independent predictions.

关键词： antineutrino flux energy spectrum reactor Daya Bay