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ACIDIC FIBROBLAST GROWTH FACTOR ReDUCeS RAT SKeLeTAL MUSCLe DAMAGe CAUSeD BY ISCHeMIA AND RePeRFUSION

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Chinese Medical Journal 1995年第3期 51-56页

作者：傅小兵 Cuevas P. Gimenez-gallego G 盛志勇田惠民 Trauma Center Postgraduate Medical College 304th Hospital. Beijing 100037 Servicio de Histologia Hospital Universitario Ramony Cajal 28034-Madrid Spain CIB-CSCI Velazquez 14428006-Madrid.Spain

Acute interruption of arterial blood flow to the extremities is often associated with significant morbidity and mortality. Broad spectrum mitogenic and non mitogenic activities of FGFs inspired us to study its protecting effects on tissue injuries in ischemia reperfusion condition. We found that systemic administration of aFGF after reperfusion onset prevented severe skeletal muscle injuries. In rats treated with aKGF, the tissue edema was reduced significantly, the tissue viability was increased, and the muscle fibers contained more succinate dehydrogenase (SDH) and adenosine triphosphatasc (ATPase). The pathological results supported the concept of improved prevention with aFGF treatment. The possible tissue protection by aFGF may come from its ability to regulate the concentration of evtra- and intracellular calcium ion. Besides, it may moderate other Ca2+ dependent enzyme conversion processes. Also, it may take part in the vascular tone regulation under ischemia and reperfusion conditions. These results suggest further study of tissue ischemia prevention with FGF and its possible mechanisms in the future.

关键词： aFGF ACIDIC FIBROBLAST GROWTH FACTOR ReDUCeS RAT SKeLeTAL MUSCLe DAMAGe CAUSeD BY ISCHeMIA AND RePeRFUSION

Automatic counting of microglial cell activation and its applications

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Neural Regeneration Research 2016年第8期11卷 1212-1215页

作者： Beatriz I.gallego Collado Pablo de Gracia Instituto de Investigaciones Oftalmologicas Ramon Castroviejo Universidad Complutense de Madrid Madrid Spain Facultad de Optica y Optometria Departamento de Oftalmologia y Otorrinolaringologia Universidad Complutense de Madrid Madrid Spain Midwestern University Chicago College of Optometry Downers Grove IL USA Department of Neurobiology Barrow Neurological Institute St. Joseph's Hospital and Medical Center Phoenix AZ USA

Glaucoma is a multifactorial optic neuropathy characterized by the damage and death of the retinal ganglion *** disease results in vision loss and *** vision loss resulting from the disease cannot be restored and nowadays there is no available cure for glaucoma; however an early detection and treatment,could offer neuronal protection and avoid later serious damages to the visual function.A full understanding of the etiology of the disease will still require the contribution of many scientific *** activation has been observed in glaucoma,being microglial proliferation a hallmark in this neurodegenerative disease.A typical project studying these cellular changes involved in glaucoma often needs thousands of images- from several animals- covering different layers and regions of the *** gold standard to evaluate them is the manual *** method requires a large amount of time from specialized *** is a tedious process and prone to human *** present here a new method to count microglial cells by using a computer *** counts in one hour the same number of images that a researcher counts in four weeks,with no loss of reliability.

关键词： glaucoma glial cells microglial cells automatic counting image processing inner plexiform layer outer plexiform layer bilateral activation

ISCHeMIA AND RePeRFUSION ReDUCe THe eNDOGeNOUS BASIC FIBROBLAST GROWTH FACTOR (bF GF) IN RATSKeLeTAL MUSCLeS

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中华医学杂志(英文版) 1995年第9期 61-65页

作者：傅小兵,Cuevas P,Gimenez-gallego G,田惠民,盛志勇 Trauma Center of Postgraduate Medical College 304th Hospital. Beijing 100037 Servicio de Histologia Hospital Universitario Ramon y Cajal 28034-Madrid Spain CIB-CSIC Velazquez 144 28006-Madrid Spain Trauma Center of Postgraduate Medical College 304th Hospital. Beijing 100037 Trauma Center of Postgraduate Medical College 304th Hospital. Beijing 100037

Polyclonal antibodies directed against human recombinant basic fibroblast growth factor (bFGF) were used in immunohistochemical studies to localize this growth factor in normal and wounded rat skeletal muscles. bFGF immunoreactivity was found mainly in the extracellular matrix, primarily in the endomysium, including the heparin-containing basal lamina and also in the capillary basal membrane of both normal and wounded muscles, however the signal intensity was much stronger in normal muscles. After 4-hour ischemia, about 40% of skeletal muscle fibers lost their bFGF immunoreactivity. Muscles which experienced 4-hour ischemia and 24 reperfusion had only a weaker bFGF immunoreactivity. The pathological results supported the concept of destroyed cell connection and fiber necrosis in ischemia and reperfused muscles. The mechanisms involved in this reduced concentration of bFGF in wounded muscles included oxygen radical activation, inflammatory response and reduced secretion of endogenous bFGF. These results w

关键词： bFGF IN RATSKeLeTAL MUSCLeS ISCHeMIA AND RePeRFUSION ReDUCe THe eNDOGeNOUS BASIC FIBROBLAST GROWTH FACTOR bF GF In PBS 回医

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声发射层析成像技术在混凝土结构中的应用

东南大学学报（自然科学版） 2014年第4期44卷 822-825页

作者：江煜许飞云许丙胜 elisabet Suárez Antolin gallego 东南大学机械工程学院南京211189 南京工业大学理学院南京210009 Granada大学应用物理系西班牙granada18072

针对传统声发射源定位算法(如时差定位)应用于各向异性介质的局限性,将声发射层析成像方法(Ae tomography)引入混凝土结构损伤定位,该算法以声发射事件作为点源,结合源定位算法和层析成像算法,通过波速变化重建物体结构缺陷(损伤)的慢度... 详细信息

针对传统声发射源定位算法(如时差定位)应用于各向异性介质的局限性,将声发射层析成像方法(Ae tomography)引入混凝土结构损伤定位,该算法以声发射事件作为点源,结合源定位算法和层析成像算法,通过波速变化重建物体结构缺陷(损伤)的慢度图,既适合于各向同性材料,也适合于各向异性材料.为了验证提出算法的可行性,制作了有和无钢筋2个不同结构的混凝土样本并通过了实验.实验结果表明所提出的方法不仅能通过声发射波速度变化实现损伤定位的可视化,而且相对于传统定位算法,混凝土样本结构缺陷(损伤)的定位精度也获得了进一步提高.

关键词：声发射源定位声发射层析成像混凝土结构重建算法

Testing for Deterministic Components in Vector Seasonal Time Series

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Open Journal of Statistics 2011年第3期1卷 145-150页

作者： José Luis gallego Carlos Díaz 不详

Certain locally optimal tests for deterministic components in vector time series have associated sampling distributions determined by a linear combination of Beta variates. Such distributions are nonstandard and must be tabulated by Monte Carlo simulation. In this paper, we provide closed form expressions for the mean and variance of several multivariate test statistics, moments that can be used to approximate unknown distributions. In particular, we find that the two-moment Inverse Gaussian approximation provides a simple and fast method to compute accurate quantiles and p-values in small and asymptotic samples. To illustrate the scope of this approximation we review some standard tests for deterministic trends and/or seasonal patterns in VARIMA and structural time series models.

关键词： Vector Time Series Deterministic Components Parametric Stability Non-Invertibility Unit Roots

实验感染肝片吸虫对水牛肝脏药物代谢酶的损伤作用

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畜牧与兽医 2001年第3期33卷 13-15页

作者：江善祥毛鑫智 J e Bayon J Gonzalez-gallego 南京农业大学动物医学院西班牙莱昂大学莱昂24071

4头 2～ 3岁健康、体重 30 0～ 40 0kg雄性去势水牛 ,经粪便检查和Dot eLISA检测确认无肝片吸虫感染后 ,一次性经口感染肝片吸虫囊蚴1 60 0个 ,研究肝片吸虫 (1次大剂量 )感染对水牛肝药酶的影响。测定血清门冬氨酸转氨酶 (AST)、谷氨... 详细信息

4头 2～ 3岁健康、体重 30 0～ 40 0kg雄性去势水牛 ,经粪便检查和Dot eLISA检测确认无肝片吸虫感染后 ,一次性经口感染肝片吸虫囊蚴1 60 0个 ,研究肝片吸虫 (1次大剂量 )感染对水牛肝药酶的影响。测定血清门冬氨酸转氨酶 (AST)、谷氨酰转肽酶 (GGT)活性及肝微粒体蛋白质、细胞色素 (Cyt)P 450、Cytb5 含量及氨基比林N 去甲基酶 (AND)、苯胺羟化酶 (AH)、UDP 葡萄糖醛酸转移酶 (UGT)和谷胱甘肽 S 转移酶(GST)等肝药酶的活性。结果表明 :肝片吸虫感染可引起AH、UGT活性下降 ,造成肝损伤。

关键词：水牛肝片吸虫肝药酶酶活性实验感染肝损伤

The Role of Demographic Dimensions on FOSS Acceptance

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通讯和计算机（中英文版） 2011年第1期8卷 8-16页

作者： Maria Dolores gallego Pereira Salvador Bueno Department of Business Administration University of Pablo de Olavide Seville 41013 Spain

Role of inflammatory response in liver diseases: Therapeutic strategies

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World Journal of Hepatology 2018年第1期10卷 1-7页

作者： José A Del Campo Paloma gallego Lourdes Grande Department of Digestive Diseases Valme University Hospital and CIBERehd

Inflammation and tumorigenesis are tightly linked pathways impacting cancer development. Inflammasomes are key signalling platforms that detect pathogenic microorganisms, including hepatitis C virus(HCV) infection, and sterile stressors(oxidative stress, insulin resistance, lipotoxicity) able to activate pro-inflammatory cytokines interleukin-1β and IL-18. Most of the inflammasome complexes that have been described to date contain a NOD-like receptor sensor molecule. Redox state and autophagy can regulate inflammasome complex and, depending on the conditions, can be either pro-or antiapoptotic. Acute and chronic liver diseases are cytokinedriven diseases as several proinflammatory cytokines(IL-1α, IL-1β, tumor necrosis factor-alpha, and IL-6) are critically involved in inflammation, steatosis, fibrosis, and cancer development. NLRP3 inflammasome gain of function aggravates liver disease, resulting in severe liver fibrosis and highlighting this pathway in the pathogenesis of non-alcoholic fatty liver disease. On the other hand, HCV infection is the primary catalyst for progressive liver disease and development of liver cancer. It is well established that HCV-induced IL-1β production by hepatic macrophages plays a critical and central process that promotes liver inflammation and disease. In this review, we aim to clarify the role of the inflammasome in the aggravation of liver disease, and how selective blockade of this main pathway may be a useful strategy to delay fibrosis progression in liver diseases.

关键词： Caspase-1 Fibrosis Hepatitis C virus Inflammasome Interleukin-1α Interleukin-1β Liver disease Non-alcoholic fatty liver disease NLRP3 Tumor necrosis factor-alpha

Local renin angiotensin system and sperm DNA fragmentation

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Asian Journal of Andrology 2022年第2期24卷 139-146页

作者： María Victoria Aparicio Prieto María Victoria Rodríguez gallego Asier Valdivia Palacín Yosu Franco Iriarte Gotzone Hervás Barbara enrique echevarría Orella Luis Casis Saenz Human Reproduction Unit Cruces University HospitalBarakaldo 48903Spain Human Reproduction Unit San Pedro HospitalLogroño 26006Spain Department of Physiology Faculty of Medicine and NursingUniversity of the Basque Country(UPV/EHU)Leioa 48940Spain Human Reproduction Unit Ruber International HospitalMadrid 28034Spain

The renin angiotensin system(RAS)appears to influence male fertility at multiple *** this work,we analyzed the relationship between the RAS and DNA *** male volunteers were divided into two groups(25 each):control(DNA fragmentation≤20%)and pathological(DNA fragmentation>20%)*** of five peptidases controlling RAS were measured fluorometrically:prolyl endopeptidase(which converts angiotensin[A]I and A II to A 1–7),neutral endopeptidase(NeP/CD10:A I to A 1–7),aminopeptidase N(APN/CD13:A III to A IV),aminopeptidase A(A II to A III)and aminopeptidase B(A III to A IV).Angiotensin-converting enzyme(A I to A II),APN/CD13 and NeP/CD10 were also assessed by semiquantitative cytometry and quantitative flow cytometry assays,as were the receptors of all RAS components:A II receptor type 1(AT1R),A II receptor type 2(AT2R),A IV receptor(AT4R or insulin-regulated aminopeptidase[IRAP]),(pro)renin receptor(PRR)and A 1–7 receptor or Mas receptor(MasR)None of the enzymes that regulate levels of RAS components,except for APN/CD13(decrease in fragmented cells),showed significant differences between both *** of RAS receptors revealed no significant differences in immunolabeling patterns between normozoospermic and fragmented *** of AT1R(94.3%normozoospermic vs 84.1%fragmented),AT4R(96.2%vs 95.3%)and MasR(97.4%vs 87.2%)was similar between the ***2R(87.4%normozoospermic vs 63.1%fragmented)and PRR(96.4%vs 48.2%)were higher in non-fragmented *** findings suggest that fragmented DNA spermatozoa have a lower capacity to respond to bioactive RAS peptides.

关键词： DNA fragmentation local renin angiotensin system sperm fertility