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Agricultural Sciences in China 2011年第6期10卷 813-819页

作者： LIN Xing-e JI Hong-qing NIU Jun-hai HU Yan-min FU Zhong-jun LIU Zong-hua TANG Ji-hua College of Agronomy Henan Agricultural University Zhengzhou 450002 P.R.China

A calcineurin B-like protein-interacting protein kinases （CIPK） gene was cloned （eU 424058） from a thermo-sensitive genic self-incompatibility （TSGI） line, He97, in maize, which was selected for suppression subtractive hybridization （SSH） library of the self-incompatible and self-compatible silks of the TSGI line under different temperatures. The full-length cDNA of the candidate CIPK-like gene consisted of 1 918 nucleotides and contained a 1 332-bp open reading frame in maize. Real-time PCR indicated that the candidate CIPK-like gene was highly expressed in the self-incompatible pollen of the TGSI line, and promoted elevated levels of calcium （Ca2＋）. High Ca2＋ concentrations in the pollen strongly inhibit pollen tube formation in silk of the same plant, thus inducing self-incompatibility （SI） under high temperature. These results implied that the CIPKs-like gene would play an important role in the regulation of He97 characteristics under different temperatures, perhaps acting as a secondary messenger in the expression of SI in the TGSI line in maize.

关键词： CIPK gene cloning expression temperature maize

Study on the Saccharification Process of Citrus Vinegar by Fruit-Grain Mixed Fermentation

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Agricultural Biotechnology 2015年第3期4卷 14-19页

作者： Pengfei CAO qing'e LIU Lishui Institute of Agricultural Science Lishui University

This study aimed to enhance acid production rate and yield of citrus vinegar, and improve the quality of liquid fermented with fruit juice. [ Method] The saccharification process of citrus vinegar was investigated preliminarily with fruit-grain mixed fermentation method using liquor starters as saccharif- erous agents. [Result] The results indicated that the optimal glutinous rice saccharification conditions were as follows： fruit-grain ratio 3： 1, Aspergillus niger- Monascus purpureus ratio 3： 1, saccharifieafion temperature 60℃, and saccharification time 2.5 h. Under the optimized saccharification conditions, reducing sugar content in saceharified glutinous rice reached 146.43 mg/ml; after fermentation, alcohol content in fermentation broth was 6%, amino nitrogen content was 0.44 mg/ml, and acid yield was 3.1 g/L. [ Conclusion] Citrus vinegar brewed based on the optimized technological conditions exhibited better sensory and physico-chemical properties than that brewed with pure juice.

关键词： Fruit-grain mixed fermentation Saecharification Liquor starter Citrus vinegar

Inhibition of acid-induced apoptosis by targeting ASIC1a mRNA with short hairpin RNA

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Acta Pharmacologica Sinica 2007年第10期28卷 1621-1627页

作者： Xie-chuan WeNG,Jian-quan ZHeNG~2,qing-e JIN,Xiao-yun MABeijing Institute of Pharmacology and Toxicology,the Academy of Military Medical Sciences,Beijing 100850,China BeijingInstituteofPharmacologyandToxicology theAcademyofMilitaryMedicalSciencesBeijing100850China

Aim:To study the role of acid-sensing ion channel (ASIC) 1a in the cell death andapoptosis induced by extracellular acid in C6 glioma ***:The stableASIC1a-silenced C6 cell line,built with RNA interference technology,were con-firmed by RT-PCR and Western blot *** cell viability following acidexposure was analyzed with lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) *** apoptoticcells dyed with Annexin-V and propidium iodide were measured with a flowcytometer,while the changes of cell cycle were also ***:Thedownregulation of ASIC1a proteins by stable transfection of short hairpin RNAdecreased the cell death percentage and increased cell viability following acidexposure with LDH and the MTT *** rate of apoptosis was lower in theASIC1a-silenced cell line than that in the wild-type C6 cell *** percentage ofsub-G0cells was lower in the ASIC1a-silenced C6 cells than that in the ***:extracellular acid induced cell death and apoptosis via ASIC1amechanisms in the C6 glioma cells.

关键词： acid-sensing ion channel RNA interference short hairpin RNA apoptosis

微动敏感床垫式睡眠监测系统诊断阻塞性睡眠呼吸暂停及睡眠参数一致性评价

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广东医学 2024年第3期45卷 287-293页

作者：梁锡丽许婷陈莉雅江茵谢晴娥廖生武冯媛南方医科大学第一临床学院广东广州510515 南方医科大学南方医院精神心理科(睡眠医学中心) 广东广州510515 南方医科大学南方医院医疗质量管理科广东广州510515 南方医科大学南方医院健康管理科广东广州510515

目的评价微动敏感床垫式睡眠监测系统(MSMSMS)在阻塞性睡眠呼吸暂停(OSA)诊断及睡眠评估中多参数与多导睡眠监测(PSG)相比的一致性。方法67例拟诊OSA的受试者同步使用PSG及MSMSMS记录多导睡眠参数,比较两者在OSA诊断及分度上的一致性,... 详细信息

目的评价微动敏感床垫式睡眠监测系统(MSMSMS)在阻塞性睡眠呼吸暂停(OSA)诊断及睡眠评估中多参数与多导睡眠监测(PSG)相比的一致性。方法67例拟诊OSA的受试者同步使用PSG及MSMSMS记录多导睡眠参数,比较两者在OSA诊断及分度上的一致性,以及分别在呼吸事件、血氧事件、睡眠基本信息及睡眠结构参数上的一致性。计量资料采取了配对t检验,Bland-Altman分析以及组内相关系数(ICC),等级资料采取了weighted kappa分析。结果MSMSMS与PSG对照,诊断轻度以上、中度以上、重度OSA的曲线下面积分别为1、0.999和0.967(Peighted kappa系数为0.841(95%CI:0.766~0.928,P<0.001)。其中呼吸暂停低通气指数(AHI)、呼吸暂停指数(AI)以及血氧指标组内相关系数分别为0.967(0.943,0.980)、0.937(0.900,0.961)、0.900(0.837,0.939)和0.936(0.891,0.962);两者在除低通气指数(HI)[0.181(-0.032,0.387),P=0.036]外的其他呼吸事件和血氧指标判读上具有很高的一致性(P<0.001);在睡眠结构的判读上,两者在浅睡眠比例和时间、深睡眠时间上具有中等程度的一致性。结论MSMSMS与PSG在OSA诊断和分度中具有高的敏感度和特异度。除了呼吸事件和血氧指标具有较强的一致性外,MSMSMS在评判OSA患者的睡眠结构上也有一定的意义。

关键词：微动敏感床垫式睡眠监测系统多导睡眠监测阻塞性睡眠呼吸暂停诊断试验一致性评价睡眠结构

A meteorologically adjusted ensemble Kalman filter approach for inversing daily emissions:A case study in the Pearl River Delta,China

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Journal of environmental Sciences 2022年第4期34卷 233-248页

作者： Guanglin Jia Zhijiong Huang Xiao Tang Jiamin Ou Menghua Lu Yuanqian Xu Zhuangmin Zhong qing’e Sha Huangjian Wu Chuanzeng Zheng Tao Deng Duohong Chen Min He Junyu Zheng School of environment and energy South China University of TechnologyUniversity Town CampusGuangzhou 510006China Institute for environmental and Climate Research Jinan UniversityGuangzhou 511486China Institute of Atmospheric Physics Chinese Academy of SciencesBeijing 100029China Faculty of Social and Behavioural Sciences Utrecht UniversityUtrechtCH 3584the Netherlands Guangzhou Institute of Tropical and Marine Meteorology China Meteorological AdministrationGuangzhou 510640China State environmental Protection Key Laboratory of Regional Air Quality Monitoring Guangdong Environmental Monitoring CenterGuangzhou 510308China Department of environmental Science and engineering College of Architecture and EnvironmentSichuan UniversityChengdu 610065China

The conventional ensemble Kalman filter(enKF),which is now widely used to calibrate emission inventories and to improve air quality simulations,is susceptible to simulation errors of meteorological inputs,making accurate updates of high temporal-resolution emission inventories *** this study,we developed a novel meteorologically adjusted inversion method(MAeInv)based on the enKF to improve daily emission *** new method combines sensitivity analysis and bias correction to alleviate the inversion biases caused by errors of meteorological *** demonstration,we used the MAeInv to inverse daily carbon monoxide(CO)emissions in the Pearl River Delta(PRD)region,*** the case study,60%of the total CO simulation biases were associated with sensitive meteorological inputs,which would lead to the overestimation of daily variations of posterior *** the new inversion method,daily variations of emissions shrank dramatically,with the percentage change decreased by 30%.Also,the total amount of posterior CO emissions estimated by the MAeInv decreased by 14%,indicating that posterior CO emissions might be overestimated using the conventional *** evaluations using independent observations revealed that daily CO emissions estimated by MAeInv better reproduce the magnitude and temporal patterns of ambient CO concentration,with a higher correlation coefficient(R,+37.0%)and lower normalized mean bias(NMB,-17.9%).Since errors of meteorological inputs are major sources of simulation biases for both low-reactive and reactive pollutants,the MAeInv is also applicable to improve the daily emission inversions of reactive pollutants.

关键词： emission inversion Daily emissions Meteorological adjustment ensemble Kalman filter

5-羟色胺选择性重摄取抑制剂联合计算机化的认知训练治疗伴有认知障碍的老年抑郁症患者的研究

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中国医刊 2024年第7期59卷 746-750页

作者：朱柯蒙汪晓周娇娇郭城伟马向林任莉张庆娥首都医科大学附属北京安定医院国家精神心理疾病临床医学研究中心精神疾病诊断与治疗北京市重点实验室北京100088

目的探讨5-羟色胺选择性重摄取抑制剂(SSRI)联合计算机化的认知训练(CCT)对伴有认知障碍的老年抑郁症(LLD)患者的临床治疗效果。方法本研究为前瞻性随机对照临床研究。选取2021年5月至2022年12月于首都医科大学附属北京安定医院门诊及... 详细信息

目的探讨5-羟色胺选择性重摄取抑制剂(SSRI)联合计算机化的认知训练(CCT)对伴有认知障碍的老年抑郁症(LLD)患者的临床治疗效果。方法本研究为前瞻性随机对照临床研究。选取2021年5月至2022年12月于首都医科大学附属北京安定医院门诊及住院治疗的118例伴有认知障碍的LLD患者,按照随机数表法分为干预组(57例)和对照组(61例)。干预组给予临床常规SSRI类抗抑郁药治疗联合CCT干预,对照组给予临床常规SSRI类抗抑郁药治疗联合空白对照方法,两组均治疗12周。分别在治疗前及治疗后8、12周对两组患者进行阿尔茨海默病认知评估量表(ADAS-cog)、老年抑郁量表(GDS)及日常生活能力量表(ADL)评分,以评估其认知功能、精神心理状况及社会功能。结果在治疗后8周及12周时,两组的ADAS-cog、GDS量表评分均明显低于治疗前,且干预组明显低于对照组,差异均有统计学意义(P0.05)。结论SSRI类抗抑郁药联合CCT治疗能够显著改善伴有认知障碍的LLD患者的认知功能和抑郁症状,可作为该类人群的有效选择。

关键词：老年抑郁症认知障碍计算机化的认知训练 5-羟色胺选择性重摄取抑制剂

Application of FRS on Target Recognition

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Open Journal of Applied Sciences 2017年第10期7卷 503-510页

作者： Donghua Gu Zhenyu Han qing’e Wu engineering Training Center Zhengzhou University of Light Industry Zhengzhou China School of electrical and Information engineering Zhengzhou University of Light Industry Zhengzhou China

In order to let machine better imitate thinking method of people to perform recognition and classification for fuzzy and uncertain thing, this paper puts forward a fuzzy and rough association method to deal with the problem. However, the application of fuzzy rough sets (FRS) will be introduced mainly on pattern recognition. Some related theories on FRS would be discussed, and some fuzzy rough mathematical methods on pattern-recognition will be given. Then, concrete applications of FRS on image processing and recognition will be introduced. Simulation result signifies that this fuzzy and rough association method is not only fast but also closer to nature attribute of thing for processing and recognizing image by comparing with the single neural network and other recognition device. The recognition rate is about 95.78%.

关键词： FR Proximity Maximum Principle of Membership Principle of Proximity Target Recognition

Applications of mercury stable isotopes for tracing volcanism in the geologic record

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Science China earth Sciences 2024年第5期67卷 1436-1458页

作者： qing GONG Ming-Xing LING Wang ZHeNG State Key Laboratory of Nuclear Resources and environment East China University of TechnologyNanchang 330013China School of earth System Science Institute of Surface-Earth System ScienceTianjin UniversityTianjin 300072China

Mercury(Hg)enrichments in ancient sediments have been used as a proxy of volcanism,especially large igneous province(LIP)***,considering the existence of other potential Hg sources besides volcanoes and the diverse factors(e.g.,organic matters,clay minerals,sulfide minerals and Fe oxides)that can affect Hg sequestration,there are considerable uncertainties to simply regard sedimentary Hg anomalies as signatures of volcanic *** stable isotopes,a promising tool for tracing the origins and transformations of Hg,have been increasingly used for determining the causes of Hg spikes and understanding the geochemical behavior of Hg in the geologic *** date,lots of researches have applied Hg concentrations and Hg isotopes to identify LIP volcanisms linking with significant geological events such as mass extinctions,ocean anoxic events and other environmental perturbations that mainly occurred in the ***,the results in previous studies clearly show that not all Hg enrichments are derived from volcanic inputs,which emphasize the need for more caution in using Hg as a fingerprint of *** a better understanding of Hg isotopes in the future,there will be important implications for Hg isotopes to reconstruct volcanic activities in the rock records and their impacts on biological evolution.

关键词： Mercury stable isotopes Large igneous province Mass extinction event Ocean anoxic event

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基于能坡划分原理的大比降山区河流阻力特性

农业工程学报 2022年第20期38卷 113-118页

作者：杨奉广彭清娥四川大学水力学与山区河流开发保护国家重点实验室成都610065 四川大学水利水电学院成都610065

在山区河流水沙灾害河道修复中,河道阻力系数是一个非常重要的参数。普通天然河道的达西阻力系数是水深h与泥沙粒径d比值的函数,而在山区大比降粗糙河道中,其值随着坡降的改变而变化。为探究大比降河道达西阻力变化规律,该研究通过变坡... 详细信息

在山区河流水沙灾害河道修复中,河道阻力系数是一个非常重要的参数。普通天然河道的达西阻力系数是水深h与泥沙粒径d比值的函数,而在山区大比降粗糙河道中,其值随着坡降的改变而变化。为探究大比降河道达西阻力变化规律,该研究通过变坡水槽试验设置3种坡度(10°、25°、35°)更大的河道,试验时床面铺设中值粒径为0.5、1和1.85 mm的3种天然沙,流量设定为0.5~2.5 L/s,淹没度范围h/d为0.84~7.27,采取试验数据和经典文献数据共计48组,涵盖河道坡度范围0.97°~35°(对应比降S范围为17‰~573.6‰),进而建立能够反映滚波影响的适用于山区大比降河流的达西阻力表达式。结果表明:1)大比降河道水流表面会产生滚波,使得达西阻力系数增大;2)大比降河流的能坡可划分为两部分:正常河道无滚波时的能坡以及河道水面滚波产生的额外能坡,前者及其对应的达西阻力系数可以利用传统的对数公式进行求取,后者可以利用总能坡与无滚波时的能坡相减得到;3)将建立的比降达西阻力公式计算结果与实测数据比较,可以发现绝大部分数据落在±20%误差线中,说明该研究提出的公式的计算精度较高。研究建立的大比降河流阻力计算模型可以揭示大比降河道水流能量消耗机理,为后续研究大比降河流问题提供理论基础。

关键词：大比降河道摩阻流速达西阻力系数淹没度滚波

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模糊自动机在电磁波识别中的应用

电子科技大学学报 2005年第6期34卷 798-801页

作者：吴青娥舒兰电子科技大学应用数学学院成都610054

研制了一个模糊处理和神经网络相结合的自动机,使用模糊技术从未处理的电磁信号波纹图像中处理模糊几何特征属性,进行波纹分类与识别。当使用模糊自动机(FA)进行识别紊乱波纹时,这些模糊几何特征是最好的。而对这些模糊几何特征的辨识,... 详细信息

研制了一个模糊处理和神经网络相结合的自动机,使用模糊技术从未处理的电磁信号波纹图像中处理模糊几何特征属性,进行波纹分类与识别。当使用模糊自动机(FA)进行识别紊乱波纹时,这些模糊几何特征是最好的。而对这些模糊几何特征的辨识,使用FA是很好的。

关键词：模糊神经网络自动机重叠信号波纹模式识别模糊几何特征