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methionine sources and genotype affect embryonic intestinal development,antioxidants,tight junctions,and growth-related gene expression in chickens

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Animal Nutrition 2024年第1期16卷 218-230页

作者： James K.Lugata Sawadi F.Ndunguru Gebrehaweria K.Reda Xénia E.Ozsváth Eszter Angyal Levente Czeglédi Gabriella Gulyás Renáta Knop János oláh Zoltán mészár Rita Varga Brigitta Csernus Csaba Szabó Department of Animal Nutrition and Physiology Faculty of Agriculture and Food Sciences and Environmental ManagementUniversity of DebrecenDebrecenHungary Department of Animal Husbandry Faculty of Agriculture and Food Sciences and Environmental ManagementUniversity of DebrecenDebrecenHungary Faculty of Agriculture and Food Sciences and Environmental management Doctoral School of Animal ScienceUniversity of DebrecenDebrecenHungary Institutes for Agricultural Research and Educational Farm University of DebrecenDebrecenHungary Department of Anatomy Histology and EmbryologyFaculty of MedicineUniversity of DebrecenDebrecenHungary Department of Evolutionary Zoology and Human Biology Faculty of Science and TechnologyUniversity of DebrecenDebrecenHungary

methionine(met)is an essential and first limiting amino acid in the poultry diet that plays a significant role in chicken embryonic development and *** present study examined the effect of in ovo injection of DL-met and L-met sources and genotypes on chicken embryonic-intestinal development and *** eggs of the two genotypes,TETRA-SL layer hybrid(TSL)—commercial layer hybrid and Hungarian Partridge colored hen breed(HPC)—a native genotype,were randomly distributed into four treatments for each *** treatment groups include the following:1)control non-injected eggs(NoIn);2)saline-injected(SaIn);3)DL-met injected(DLm);and 4)L-met injected(Lm).The in ovo injection was carried out on 17.5 d of embryonic development;after hatching,eight chicks per group were sacrificed,and the jejunum was extracted for *** results showed that both DLm and Lm groups had enhanced intestinal development as evidenced by increased villus width,villus height,and villus area(Pmpared to the *** DLm group had significantly reduced crypt depth,glutathione(GSH)content,glutathione S-transferase 3 alpha(GST3),occludin(OCLN)gene expression and increased villus height to crypt depth ratio in the TSL genotype than the Lm group(PmD2),occludin(OCLN),superoxide dismutase 1(SOD1),and GST3 genes than the TSL genotype(Pmet enhanced intestinal development,and function,with genotypes responding differently under normal *** also influenced the expression of intestinal antioxidants,tight junction,and growth-related genes.

关键词： DL-methionine L-methionine In ovo injection Intestinal development Poultry

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Rilmenidine prevents blood pressure increase in rats with compromised nitric oxide production

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Acta Pharmacologica Sinica 2004年第12期25卷 74-80页

作者： mria GEROV,Jozef TRK,ol’ga PECHV,Jana mATUKOV Institute ofNormal and Pathological Physiology SlovakAcademy of Sciences 813 71 Bratislava Slovak Republic Institute ofNormal and Pathological Physiology SlovakAcademy of Sciences 813 71 Bratislava Slovak Republic Institute ofNormal and Pathological Physiology SlovakAcademy of Sciences 813 71 Bratislava Slovak Republic Institute of Pathophysiology medical School Comenius University Bratislava Slovak Republic Now in Center for Blood Research and Department of Pathology Harvard medicalSchool Boston USA.

AIm: To search tools of high blood pressure in the model of nitric oxide (NO)-defective hypertension, and the study focused on the effect of rilmenidine, agonist of imidazoline receptors, which was suggested to modulate central sympathetic outflow. mETHODS: Three experimental groups, each consisting of 7 rats, were used: (I) rats with inhibition of NO synthase (NOS) by NG-nitro-L-arginine methyl ester (L-NAmE) 40 mg·kg-1·d-1 for 4 weeks in drinking water, (II) rats with inhibited NOS as in group I , plus agonist of imidazoline receptors rilmenidine 3 mg·kg-1·d-1 for 4weeks by gavage, and(III) control rats. Systolic blood pressure wasmeasured weekly noninvasively. At the end of experiment aortic ring isometric tension was followed, NOS expression (aorta, left ventricle), and NOS activity (left ventricle and brain) were determined. RESULTS: In the group I systolic blood pressure in- creased significantly, aortic ring relaxation to acetylcholine was significantly attenuated. Rilmenidine administered simultaneously with L-NAmE (group II) prevented the increase of blood pressure which did not differ significantly from control values; aortic ring relaxation to acetylcholine did not differ from control. No change in NOS expres- sion (aorta and left ventricle) was found in groups I and II. Significant decline in NOS activity (left ventricle and brain) wasfound in groups I and II. CONCLUSION: Rilmenidinehas aremarkable role inNO-defective hypertension, possibly by inhibiting central sympathetic outflow and by affecting receptors in vascular smooth muscle also. The prime cause of hypertension in this experimental model - the compromised production of NO due to inhibition of NOS - was not affected by rilmenidine.

关键词： NO deficiency hypertension imidazoline receptor nitric-oxide synthase sympathetic activity

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Highly Heat-Resistant materials for microelectronics:Hydrodynamic,Optical,and Conformational Properties of Fluorine Containing Poly(Amido-ortho-Hydroxy Amide)

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Journal of Wuhan University of Technology(materials Science) 2020年第1期35卷 223-230页

作者： Irina A Strelina Liudmila I Rudaya ol’ga V Okatova maxim N Bol’shakov Aleksey Yumarfichev Galina K Lebedeva Yuliya V Shagayko Stanislav m Ramsh Institute of macromolecular Compounds Russian Academy of SciencesBolshoi pr.VO 31St Petersburg199004 Russia Saint-Petersburg State Institute of Technology(Technical University) Moskovskii pr.26St Petersburg190013 Russia

The polycondensation of the mixture of diamines 5,5-methylene bis(2-aminophenol) and 4,4-(hexafluoroisopropylidene)dianiline(molar ratio 0.8:0.2) with isophthaloyl dichloride was used to synthesize poly(amido-o-hydroxy amide)(POA-F)-new heat resistant binder of the composites for microelectronics. The copolymer was fractionated, its hydrodynamic, optical, and conformational properties were researched, and molecular masses(ММ) of the fractions were defined. The polydispersity index was estimated. Based on experimental data, calculation of the size of a segment of Kuhn characterizing degree of an intramolecular orientation order and value of coefficients of the equation of mark-Kuhn-Hauvink for viscometric and diffusion data were performed. It was demonstrated that introducing 20 mol % of the monomer with-CF3-groups does not lead to any changes in conformational properties of the macromolecules and does not change the degree of intramolecular orientational order(the Kuhn segment length). Optical characteristics of POA-F solutions are virtually similar to the corresponding values for POA prepared with the use of single amine-containing component-5,5-methylene bis(2-aminophenol). The received mm distribution for POA-F(prepolymer) provided the solubility of its films in alkaline solutions. The heat resistance(τ5 and τ10-temperatures corresponding to 5% and of 10% PBO-F mass loss of a polymer) of the powders and the films of PBO, PBO-F were determined. The electrophysical parameters-dielectric permittivity(ε) and dielectric loss tangent(tan δ) of the PBO-F films decreased down to 3.30 and 0.017, in comparison 3.40 and 0.025 for PBO respectively.

关键词： poly(o-hydroxy amide) 4,4-(hexafluoroisopropylidene) dianiline Kuhn segment mark-Kuhn-Houwink equations translational diffusion intrinsic viscosity flow birefringence

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Discrepancies between clinical and autopsy findings in patients who had an acute stroke

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Stroke & Vascular Neurology 2022年第3期7卷 215-221页

作者： Lilla Hudák Attila Csaba Nagy Sarolta molnár Gábor méhes Katalin Erzsébet Nagy Lászlóoláh LászlóCsiba Department of Neurology University of Debrecen Faculty of MedicineDebrecenHungary Department of Preventive medicine University of Debrecen Faculty of Public HealthDebrecenHungary Department of Pathology University of Debrecen Clinical CenterDebrecenHungary Doctoral School of Neuroscience University of Debrecen Faculty of MedicineDebrecenHungary

Objectives According to international observations,the incidence of clinical autopsies is declining worldwide,plummeting below 5%in the USA and many European *** is an unfavourable trend as,in 7%-12%of cases,recent clinicopathological studies found discrepancies that might have changed the therapy or the outcome if known *** previous large-scale observations have examined varied patient populations,we aimed to focus on the differences between the clinical and pathological diagnostic findings in only patients who had a *** and methods We assessed the postmortem non-neuropathological and neuropathological findings of 534 consecutive patients who had a stroke who passed *** neoplasms,pneumonias,thromboembolisms and haemorrhagic transformations revealed only by autopsy were considered severe abnormalities;in addition,benign abnormalities important from an educational or scientific point of view were also *** In 26 of the 534 cases(4.9%),the presence of systemic neoplasms had already been confirmed in the clinical stage;however,8(1.5%)malignant tumours were only detected during ***,80(15%)thromboembolic events,73(13.6%)pneumonias and 66(18%)haemorrhagic transformations were only diagnosed at *** hospital stay(from admission to death)resulted in fewer discrepancies between clinical and autopsy diagnosis of thromboembolic events and pneumonias(pmaging techniques,postmortem autopsies are also important in patients who had a stroke as autopsies may reveal clinically silent diseases(eg,tumour),and contribute to knowing the actual incidence of stroke-related thromboembolic and pneumonia complications.

关键词： neoplasms clinical finding

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mechanical Properties of Living Adherent Cells :Relationship with Structure and Function

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生物医学工程学杂志 2005年第S1期22卷 9-10页

作者： R. FODIL S. Féréol E. PLANUS V.m. LAURENT B. LOUIS D. ISABEY Inserm UmR 651 Fonctions Cellulaire et Moléculaire de l'Appareil Respiratoire et des Vaisseaux Equipe Biomécanique Cellulaire et Respiratoire Faculté de Médecine Créteil 94 010 FranceInserm UMR 651 Fonctions Cellulaire et Moléculaire de l'Appareil Respiratoire et des Vaisseaux Equipe Biomécanique Cellulaire et Respiratoire Faculté de Médecine Créteil 94 010 FranceInserm UMR 651 Fonctions Cellulaire et Moléculaire de l'Appareil Respiratoire et des Vaisseaux Equipe Biomécanique Cellulaire et Respiratoire Faculté de Médecine Créteil 94 010 FranceInserm UMR 651 Fonctions Cellulaire et Moléculaire de l'Appareil Respiratoire et des Vaisseaux Equipe Biomécanique Cellulaire et Respiratoire Faculté de Médecine Créteil 94 010 FranceInserm UMR 651 Fonctions Cellulaire et Moléculaire de l'Appareil Respiratoire et des Vaisseaux Equipe Biomécanique Cellulaire et Respiratoire Faculté de Médecine Créteil 94 010 FranceInserm UMR 651 Fonctions Cellulaire et Moléculaire de l'Appareil Respiratoire et des Vaisseaux Equipe Biomécanique Cellulaire et Respiratoire Faculté de Médecine Créteil 94 010 France

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Diel differences in blue whale(Balaenoptera musculus)dive behavior increase nighttime risk of ship strikes in northern Chilean Patagonia

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Integrative Zoology 2021年第4期16卷 594-611页

作者： Francesco CARUSO Leigh HICKmOTT Joseph DWARREN Paolo SEGRE Gustavo CHIANG Paulina BAHAmONDE Sonia ESPAÑol-JImÉNEZ Songhai LI Alessandro BOCCONCELLI marine mammals and marine Bioacoustics Laboratory Institute of Deep-sea Science and EngineeringChinese Academy of Sciences(CAS)SanyaChina Woods Hole Oceanographic Institution Woods HoleMassachusettsUSA Open Ocean Consulting HampshireUK Scottish Oceans Institute University of St AndrewsSt AndrewsUK Stony Brook University SouthamptonNew YorkUSA Hopkins marine Station Stanford UniversityPacific GroveCaliforniaUSA melimoyu Ecosystem Research Institute Santiago de ChileChile HUB Ambiental–Centro de Estudios Avanzado Universidad de Playa Ancha(UPLA)ValparaísoChile

The northern Chilean Patagonia region is a key feeding ground and a nursing habitat in the southern hemisphere for blue whales(Balaenoptera musculus).From 2014 to 2019,during 6 separate research cruises,the dive behavior of 28 individual blue whales was investigated using bio-logging tags(DTAGs),generating≈190 h of *** dove to significantly greater depths during the day compared to nighttime(day:32.6±18.7 m;night:6.2±2.7 m;Pmost time was spent close to the surface(86%±9.4%;Pmultaneously with whale diving *** whales appeared to perform dives relative to the vertical migration of prey during the *** association between diurnal prey migration and shallow nighttime dive behavior suggests that blue whales are at increased risk of ship collisions during periods of darkness since the estimated maximum ship draft of vessels operating in the region is also≈12 *** recent decades,northern Chilean Patagonia has seen a large increase in marine traffic due to a boom in salmon aquaculture and the passenger ship *** strike risks for large whales are likely underestimated in this *** reported in this study may be valuable for policy and mitigation decisions regarding conservation of the endangered blue whale.

关键词： bio-logging tags blue whale diving profile ocean conservation prey distribution

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Ethyl and butyl acetate oxidation over manganese oxides

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Chinese Journal of Catalysis 2018年第1期39卷 27-36页

作者： olívia Salomé G.P.Soares Raquel P.Rocha Jose J.m.Orfao manuel F.R.Pereira José L.Figueiredo Laboratory of Separation and Reaction Engineering‐Laboratory of Catalysis and materials (LSRE‐LCm) Faculty of Engineering University of Porto Rua Dr. Roberto Frias s/n 4200‐465 Porto Portugal

mangenese oxides were synthesized using two new methods,a novel solvent‐free reaction and a reflux technique,that produced cryptomelane‐type products(K‐OmS‐2).Oxides were also synthesized using conventional methods and all specimens were applied to the oxidation of ethyl acetate and butyl acetate,acting as models for the volatile organic compounds found in industrial *** catalysts were also characterized using N2adsorption,X‐ray diffraction,scanning electron microscopy,temperature programmed reduction and X‐ray photoelectron *** of the manganese oxides was found to be very active during the oxidation of both esters to CO2,and the synthesis methodology evidently had a significant impact on catalytic *** K‐OmS‐2nanorods synthesized by the solvent‐free method showed higher activity than K‐OmS‐2materials prepared by the reflux technique,and samples with cryptomelane were more active than those prepared by the conventional *** catalyst with the highest performance also exhibited good stability and allowed90%conversion of ethyl and butyl acetate to CO2at213and202°C,*** differences in the catalyst performance were observed,clearly indicating that K‐OmS‐2nanorods prepared by the solvent‐free reaction were better catalysts for the selected VOC oxidations than the mixtures of manganese oxides traditionally obtained with conventional synthesis *** superior performance of the K‐OmS‐2catalysts might be related to the increased average oxidation state of the manganese in these *** correlations between the catalytic performance and the surface chemical properties were also identified,hig-hlighting the K‐OmS‐2properties associated with the enhanced catalytic performance of the materials.?2018,Dalian Institute of Chemical Physics,Chinese Academy of *** by Elsevier *** rights reserved.

关键词： Volatile organic compound Ethyl acetate Butyl acetate manganese oxide Catalytic oxidation

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A Dual Effect of Au-Nanoparticles on Nucleic Acid Cholesteric Liquid-Crystalline Particles

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Journal of Biomaterials and Nanobiotechnology 2011年第4期2卷 461-471页

作者： Yuri m. Yevdokimov Sergey G. Skuridin Victor I. Salyanov Vladimir I. Popenko Victor m. Rudoy ol,ga V. Dement′eva Eleonora V. Shtykova 不详

Au-nanoparticles (size about 2 nm, but not 5 or 15 nm) are capable of effectively incorporating into quasinematic layers of particles of cholesteric liquid-crystalline dispersion formed by double-stranded nucleic acid molecules of various families (DNA and poly(I)xpoly(C)). This Au-size-dependent process is accompanied by a decrease in amplitudes of abnormal bands in the CD spectra specific to initial cholesteric liquid-crystalline dispersions and simultaneously by an appearance of plasmon resonance band in visible absorption spectrum. The study of properties of particles of cholesteric liquid-crystalline dispersion treated with Au-nanoparticles by means of various physico-chemical methods demonstrates that incorporation of Au-nanoparticles into quasinematic layers of these particles results in two effects: i) it facilitates reorganization of the spatial cholesteric structure of particles, and ii) it induces the formation of Au-clusters in the content of particles. It is not excluded that these effects represent a possible reason for genotoxicity of Au-nanopar- ticles.

关键词： Double-Stranded Nucleic Acids Gold Nanoparticles Nucleic Acid Liquid-Crystalline Dispersions Circular Dichroism Structure of Lyotropic Liquid Crystals of (Bio)Polymers Genotoxity of Nanoparticles

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日本高中地理科目课程改革前后的比较

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外国教育研究 2001年第4期28卷 50-53页

作者：兰婷四平师范学院初等教育学院吉林四平136000

本文依据日本 1 999年颁布的新的高中学习指导要领 ,对高中地理科目课程改革前后的教育目标、教育内容等方面进行比较。

关键词：高中地理科目课程改革教育目标教育内容日本

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集约化蛋鸡场鸡粪混合液资源化无害化处理

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城市环境与城市生态 1992年第1期5卷 1-6页

作者：朱锦福同济大学环境工程学院

本研究采用离心分离法、高温好氧发酵法和中温厌氧发酵对鸡粪混合液进行资源化、无害化综合处理。实验结果:鸡粪回收率53.0%～91.8%;发酵后,鸡粪含水率31%～44%;废水厌氧处理的体积负荷12.14kg·COD_(cr)/m^3·d;产沼比0.52m^3/kg·COD_... 详细信息

本研究采用离心分离法、高温好氧发酵法和中温厌氧发酵对鸡粪混合液进行资源化、无害化综合处理。实验结果:鸡粪回收率53.0%～91.8%;发酵后,鸡粪含水率31%～44%;废水厌氧处理的体积负荷12.14kg·COD_(cr)/m^3·d;产沼比0.52m^3/kg·COD_(cr);甲烷含量>70%。通过综合处理,COD_cr)、BOD_5的总去除率均大于90%。实验表明,本工艺具有投资少、能耗低、费用省和效益比较明显等优点。

关键词：鸡粪资源化厌氧发酵无害化处理

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