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A direct,sensitive and high-throughput genus and species-specific molecular assay for large-scale malaria screening

Infectious Diseases of Poverty 2022年第2期11卷 11-22页

作者： Yaling Zhao Ye Zhao Yu Sun Lihua Fan duoquan wang Heng wang Xiaodong Sun Zhi Zheng Institute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijingChina Yunnan Institute of Parasitic Diseases Yunnan Center for Malaria ResearchInstitute of Vector and Pathogen BiologyDali UniversityPuerYunnanChina National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionWHO Collaborating Center for MalariaSchistosomiasisand FilariasisKey Laboratory of Parasite and Vector BiologyMinistry of HealthShanghaiChina Blood Transfusion Department Peking Union Medical College HospitalChinese Academy of Medical SciencesBeijingChina

Background:Infectious disease diagnostics often requires sensitive molecular assays that identify at both genus and species levels.For large scale screening,such as malaria screening for elimination,diagnostic assay can be a challenge,as both the throughput and cost of the assay must be considered.The requirement of nucleic acid extraction hampers the throughput of most molecular assays.Co-amplification of multiple species or multiplex identification either can result in missed diagnosis or are too costly for large-scale screening.A genus-and species-specific diagnostic assay with simplified procedure,high sensitivity and throughput is still needed.This study aimed to develop a sensitive and high-throughput approach for large-scale infectious disease screening.Methods:We developed multi-section Capture and Ligation Probe PCR(mCLIP-PCR)for the direct detection of RNA without extraction and reverse transcription.Multiple tailed sandwich hybridization probes were used to bind at genus-and species-specific sections of the target RNA to cooperatively capture the target onto a 96-well plate.After enzymatic ligation of the bound probes,a single-stranded DNA formed at each section with distinct tail sequence at the ends.They were separately PCR-amplified with primers corresponding to tail sequences for genus or species identification.We applied the method to the active screening ofPlasmodium infections of 4,580 asymptomatic dried blood spot samples collected in malaria endemic areas and compared the results with standard qPCR using linear regression.Results:With multi-section cooperative capture but separate amplification strategy,we accurately identified genusPlasmodium and speciesP.falciparum andP.vivax without RNA extraction,with favorable sensitivities among the published reports.In the active screening,our method identified all 53 positive infections including two mixed infections,and twoP.vivax infections that were missed by standard qPCR.Conclusions:mCLIP-PCR provides a sensitive and high-throughput approach to la

关键词： Infectious disease Malaria Molecular screening CLIP-PCR Genus Species High-throughput

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Development of innovative tripartite partnership for China’s engagement in global health: recommendations from China-Tanzania Cooperation Project on Malaria Control

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贫困所致传染病(英文) 2024年第2期13卷 1-3页

作者： Ma Xuejiao Lu Shenning Ding Wei Deng Shanying wang duoquan Xiao Ning Mlacha Yeromin Husain Lewis Zhou Xiaonong National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research) NHC Key Laboratory of Parasite and Vector Biology WHO Collaborating Center for Tropical Diseases National Center for International Research On Tropical Diseases Shanghai China University of Hong Kong Hong Kong China School of Global Health Chinese Center for Tropical Diseases Research Shanghai Jiao Tong University School of Medicine Shanghai China Ifakara Health Institute Dar-es-Salaam Tanzania Institute of Development Studies Brighton UK

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Role of international network on surveillance and response system leading to malaria elimination: China’s engagement in global health

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Infectious Diseases of Poverty 2022年第3期11卷 63-68页

作者： Shenning Lu Lulu Huang Lei Duan Qiuli Xu Xuejiao Ma Wei Ding duoquan wang Shan Lv Ning Xiao Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research) NHC Key Laboratory of Parasite and Vector BiologyWHO Collaborating Centre for Tropical DiseasesNational Center for International Research On Tropical DiseasesNational Institute of Parasitic DiseasesShanghai200025China Pudong New Area Center for Disease Control and Prevention and Pudong Institute of Preventive Medicine Fudan UniversityPudong New AreaShanghai200136China School of Global Health Chinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghai200025China State Key Laboratory of Genetic Engineering Ministry of Education Key Laboratory for Biodiversity Science and Ecological EngineeringMinistry of Education Key Laboratory of Contemporary AnthropologyDepartment of Infectious DiseasesSchool of Life ScienceHuashan HospitalFudan UniversityShanghai200433China

China has accumulated multiple practices and experiences in building and enhancing malaria surveillance and response system. As China’s engagement into global health has gathered stronger momentum than ever, China together with the Swiss Tropical and Public Health Institute and WHO has organised five sessions of the International Forum on Surveillance-Response System Leading to Tropical Diseases Elimination during 2012–2020, in which malaria elimination has always been one of the hottest topics. In this study, the roles of international network on the surveillance and response system were explored to achieve a global malaria-free goal. China’s approach to malaria elimination has demonstrated significance of global collaboration on taking joint prevention and control, and building a worldwide institutional-based network.

关键词： Surveillance and response system Malaria Elimination Network

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Evaluation of Malaria Standard Microscopy and Rapid Diagnostic Tests for Screening—Southern Tanzania,2018–2019

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China CDC weekly 2022年第28期4卷 605-608页

作者： Kangming Lin Mei Li duoquan wang Fei Luo Shenning Lu Mihayo GMichael Yeromin Mlacha Prosper Chaki Ning Xiao Xiao-nong Zhou Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention Nanning CityGuangxi Zhuang Autonomous RegionChina National Institute of Parasitic Diseases Chinese Center for Tropical Diseases ResearchChinese Center for Disease Control and PreventionNHC Key Laboratory of Parasite and Vector BiologyWHO Collaborating Centre for Tropical DiseasesNational Center for International Research on Tropical DiseasesSchool of Global HealthChinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghai MunicipalityChina Chongqing City Center for Disease Control and Prevention Chongqing MunicipalityChina Ifakara Health Institute Dares Salaamthe United Republic of Tanzania.

Summary What is already known about this topic?Microscopy is the gold standard for parasitological confirmation,but the accuracy of microscopic diagnosis is influenced by the skill of the technicians.An alternative is the immunologic-based malaria rapid diagnostic tests(mRDTs).What is added by this report?Our study evaluated standard microscopy in health system(SMHS)and mRDTs for focused screening and treatment of malaria(FSAT)in Southern Tanzania.We showed that mRDTs were more sensitive than local SMHS for diagnosing malaria infection.What are the implications for public health practices?Malaria rapid diagnostic tests can be useful as an alternative to SMHS for FSAT in the local context of Tanzania.

关键词： Malaria diagnosis confirmation

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The “Paired Learning by Doing” Approach for Capacity Building Derived from the China-UK-Tanzania Pilot Project on Malaria Control During 2015–2018

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China CDC weekly 2023年第31期5卷 694-697页

作者： Shenning Lu Xuejiao Ma Wei Ding duoquan wang Zhengbin Zhou Kangming Lin Fei Luo Shan Lyu Ning Xiao Xiao-nong Zhou National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)NHC Key Laboratory of Parasite and Vector BiologyWHO Collaborating Centre for Tropical DiseasesNational Center for International Research on Tropical DiseasesShanghaiChina National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)NHC Key Laboratory of Parasite and Vector BiologyWHO Collaborating Centre for Tropical DiseasesNational Center for International Research on Tropical DiseasesSchool of Global HealthChinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghaiChina Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention Nanning CityGuangxi Zhuang Autonomous RegionChina Chongqing Center for Disease Control and Prevention.Chongqing China.

China’s growing prosperity has led to an increased role in international affairs,particularly in global health cooperation.Alongside sending medical teams abroad,China’s health authority is gradually expanding its South-South cooperation in public health.However,due to a late start in global health initiatives and limited experience in organizing,coordinating,and implementing intervention projects overseas,China still faces significant challenges in global health,particularly in the local context.Recognizing the longstanding partnership between the Chinese and British governments,they have identified global health as a new area of strategic cooperation.In 2012,the former UK Department for International Development(DFID)initiated a new type of health development cooperation project named the China-UK Global Health Support Programme(GHSP).

关键词： initiated expanding cooperation

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Profile and determinants of delayed care-seeking and diagnosis among patients with imported malaria: a retrospective study in China, 2014–2021

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Infectious Diseases of Poverty 2022年第6期11卷 50-59页

作者： Tao Zhang duoquan wang Yingjun Qian Wei Ruan Ying Liu Jing Xia Hui Yan Yuan Sui Shenning Lu Xian Xu Jingjing Jiang Xiaofeng Lyu Shuqi wang Shizhu Li Weidong Li Anhui Provincial Center for Disease Control and Prevention Hefei 230601China National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)NHC Key Laboratory of Parasite and Vector BiologyWHO Collaborating Center for Tropical DiseasesNational Center for International Research on Tropical DiseasesShanghai 200025China Zhejiang Provincial Center for Disease Control and Prevention Hangzhou 310051China Henan Provincial Center for Disease Control and Prevention Zhengzhou 450016China Hubei Provincial Center for Disease Control and Prevention Wuhan 430079China Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention Nanning 530028China Brown School Washington UniversitySt.LouisMOUSA School of Global Health Chinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghai 200025China

Background: In areas where malaria has been eliminated, delayed care-seeking and diagnosis of imported malaria are constant threats. This study aimed to describe the profile and determinants of delayed care-seeking and diagnosis among patients with imported malaria in China.Methods: This retrospective study assessed surveillance data obtained from 2014 to 2021 in the Chinese provincial-level administrative divisions (PLADs) of Anhui, Henan, Hubei, and Zhejiang, and Guangxi. Epidemiological characteristics were analyzed using descriptive statistics. Furthermore, factors associated with delayed care-seeking and diagnosis among imported malaria cases were identified using multivariate logistic regression.Results: Overall, 11.81% and 30.08% of imported malaria cases had delays in seeking care and diagnosis, respectively. During the study period, there was a decreasing trend in the proportion of imported malaria cases with delayed care-seeking (χ^(2) = 36.099,P < 0.001) and diagnosis (χ^(2) = 11.395,P = 0.001). In multivariate analysis, independent risk factors associated with delayed care-seeking include PLADs (Guangxi as reference), consultations in high-level facilities for the first medical visit, infections with non-Plasmodium falciparum species, and older age. However, PLADs (Guangxi as reference), the purpose of traveling (labour as reference), and infections with non-P. falciparum species increased the risk of delayed diagnosis. Delayed care-seeking (adjusted odds ratio: 1.79,P = 0.001) and diagnosis (adjusted odds ratio: 1.62,P = 0.004) were risk factors for severe disease development.Conclusions: Based on this study’s findings, we strongly advocate for improved access to quality healthcare to reduce the rate of misdiagnosis at the first visit. Infections caused by non-P. falciparum species should be highlighted, and more sensitive and specific point-of-care detection methods for non-P. falciparum species should be developed and implemented. In addition, education programs should be enhanced to reach target p

关键词： Malaria Delayed care-seeking Delayed diagnosis Imported malaria Prevention Re-establishment China Plasmodium falciparum

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Assessment of tree-ring mercury radial translocation and age effect in Masson pine:Implications for historical atmospheric mercury reconstruction

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Journal of Environmental Sciences 2024年第4期138卷 266-276页

作者： Xu Liu Xun wang Dingyong wang College of Resources and Environment Southwest UniversityChongqing 400715China State Key Laboratory of Environmental Geochemistry Institute of GeochemistryChinese Academy of SciencesGuiyang 550081China

The tree ring has been regarded as an emerging archive to reconstruct historical atmospheric mercury(Hg)trends,but with the large knowledge gaps in the reliability.In this study,we comprehensively evaluated the Hg source,radial translocation and age effect of Masson pine(Pinus massoniana)tree ring at Mt.Jinyun in Chongqing,to assess the suitability of such tree ring as the archive of atmospheric Hg.Results showed that distinct variabilities among Masson pine tree-ring Hg concentration profiles.The Hg concentration significantly increased along with stem height(P<0.05),indicating the Hg in tree rings mainly derived from foliage uptake atmospheric Hg.We found a distinct age effect that the tree ring of young trees had the higher Hg concentration.Besides,we used the advection-diffusion model to demonstrate how Hg concentration shifted by the advection or/and diffusion in tree rings.The modeling results showed that the advection induced radial translocation during the young growth period of tree was a plausible mechanism to result in the tree-ring Hg record largely different from the trend of anthropogenic Hg emissions in Chongqing.We finally suggest that in further Hg dendrochemistry,better discarding the tree-ring Hg profile of the young growth period to reduce impacts of the radial translocation and age effect.

关键词： Masson pine Tree-ring mercury Radial translocation Tree age effect

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Pushing the boundaries: an interview with Dae-Hyun Kim on terahertz devices

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National Science Review 2024年第3期11卷 42-45页

作者： Defu wang the Center for Carbon-based Electronics School of Electronics Peking University

Ⅲ–Ⅴ compound semiconductors, such as InGaAs/InAlAs, exhibit exceptional carrier transport properties, establishing them as fundamental elements in terahertz(THz) applications crucial for the development of 6G netwo...

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Enrichment of anammox biomass during mainstream wastewater treatment driven by achievement of partial denitrification through the addition of bio-carriers

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Journal of Environmental Sciences 2024年第3期137卷 181-194页

作者： Yuqing Ma Bo wang Xiaodi Li Shuo wang Wen wang Yongzhen Peng National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology Engineering Research Center of BeijingBeijing University of TechnologyBeijing 100124China

Anammox is widely considered as the most cost-effective and sustainable process for nitrogen removal.However,how to achieve the enrichment of anammox biomass remains a challenge for its large-scale application,especially in mainstream wastewater treatment.In this study,the feasibility of enrichment of anammox biomass was explored through the realization of partial denitrification and the addition of bio-carriers.By using ordinary activated sludge,a sequencing batch reactor(SBR)followed by an up-fow anaerobic sludge bed(UASB)was operated at 25±2℃ for 214 days.The long-term operation was divided into five phases,in which SBR and UASB were started-up in Phases I and II,respectively.By eliminating oxygen and adjusting the infow ratios in Phases III-V,advanced nitrogen removal was achieved with the effuent total nitrogen being 4.7 mg/L and the nitrogen removal efficiency being 90.5%in Phase V.Both in-situ and ex-situ activity tests demonstrated the occurrence of partial denitrification and anammox.Moreover,16S rRNA high-throughput sequencing analysis revealed that Candidatus Brocadia was enriched from below the detection limit to in biofilms(0.4%in SBR,2.2%in UASB)and the foc sludge(0.2%in SBR,1.3%in UASB),while Thauera was mainly detected in the foc sludge(8.1%in SBR,8.8%in UASB),which might play a key role in partial denitrification.Overall,this study provides a novel strategy to enrich anammox biomass driven by rapid achievement of partial denitrification through the addition of bio-carriers,which will improve large-scale application of anammox processes in mainstream wastewater treatment.

关键词： Enrichment Anammox biomass Bio-carriers Partial denitrification Mainstream wastewater

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Performance and mechanism of La-Fe metal-organic framework as a highly efficient adsorbent for fluoride removal from mine water

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Journal of Environmental Sciences 2024年第5期139卷 245-257页

作者： Chaomin Jia Jianbing wang Huijiao wang Sichao Zhu Xiaohui Zhang Yuxiang wang School of Chemical&Environmental Engineering China University of Mining&Technology-BeijingBeijing 100083China Beijing Polytechnic Beijing 100176China Chinese Society for Urban Studies Beijing 100835China

Water fluoride pollution has caused non-negligible harm to the environment and humans,and thus it is crucial to find a suitable treatment technology.In this study,La-Fe@PTA adsorbent was synthesized for the defluoridation of mine water.The results showed that the optimum conditions for defluoridation by La-Fe@PTA were p H close to 7.0,the initial F-concentration of 10 mg/L,the dosage of 0.5 g/L and the adsorption time of 240 min.Compared with SO_4^(2-),Cl^(-),NO_(3)^(-),Ca^(2+)and Mg^(2+),CO_(3)^(2-)and HCO_(3)^(-)presented severer inhibition on fluoride uptake by La-Fe@PTA.The adsorption process fits well with the pseudo-second-order kinetic model and Freundlich model,and the maximum adsorption capacity of Langmuir model was 95 mg/g.Fixed-bed adsorption results indicated that fluoride in practical fluorinated mine water could be effectively removed from 3.6 mg/L to less than 1.5 mg/L within130 bed volume(BV)by using 1.5 g La-Fe@PTA.Furthermore,the adsorbent still had good adsorption capacity after regeneration,which confirms the great application potential of La-Fe@PTA as a fluoride ion adsorbent.The mechanism analysis showed that La-Fe@PTA adsorption of fluorine ions is a physicochemical reaction driven by electrostatic attraction and ion exchange.

关键词： Adsorption La-Fe@PTA Fluoride ion Mine water Mechanism

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