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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第6期10卷 479-482页

作者： Bao-zhong ZHANG Xin ZHANG Xiao-ping AN duo-liang ran Yu-sen ZHOU Jun LU Yi-gang TONG State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 100071 China College of Veterinary Science Xinltang Agricultural University Urumqi 830052 China Tumor Biotherapy Wurd Beijing YouAn Hospital Capital Medical University Beijing 100069 China

Site-directed mutagenesis (SDM) has been a very important method to probe the function-structure relationship of proteins. In this study, we introduced an easy-to-use, polymerase chain reaction (PCR)-based SDM method for double-stranded plasmid DNA, with a designed restriction site to ensure simple and efficient mutant screening. The DNA sequence to be mutated was first translated into amino acid sequence and then the amino acid sequence was reversely translated into DNA sequence with degenerate codons, resulting in a large number of sequences with silent mutations, which contained various restriction endonu-clease (RE) sites. Certain mutated sequence with an appropriate RE site was selected as the target DNA sequence for designing a pair of mutation primers to amplify the full-length plasmid via inverse PCR. The amplified product was 5′-phosphorylated, cir-cularized, and transformed into an Escherichia coli host. The transformants were screened by digesting with the designed RE. This protocol uses only one pair of primers and only one PCR is conducted, without the need for hybridization with hazardous isotope for mutant screening or subcloning step.

关键词： Site-directed mutagenesis (SDM) Restriction endonuclease Mutant screening

Design strategies and insights of flexible infrared optoelectronic sensors

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Journal of Semiconductors 2025年第1期46卷 21-56页

作者： Yegang liang Wenhao ran Dan Kuang Zhuoran Wang School of Integrated Circuits and Electronics Beijing Institute of TechnologyBeijing 100081China

Infrared optoelectronic sensing is the core of many critical applications such as night vision,health and medication,military,space exploration,*** including mechanical flexibility as a new dimension enables novel features of adaptability and conformability,promising for developing next-generation optoelectronic sensory applications toward reduced size,weight,price,power consumption,and enhanced performance(SWaP^(3)).However,in this emerging research frontier,challenges persist in simultaneously achieving high infrared response and good mechanical deformability in devices and integrated ***,we perform a comprehensive review of the design strategies and insights of flexible infrared optoelectronic sensors,including the fundamentals of infrared photodetectors,selection of materials and device architectures,fabrication techniques and design strategies,and the discussion of architectural and functional integration towards applications in wearable optoelectronics and advanced image ***,this article offers insights into future directions to practically realize the ultra-high performance and smart sensors enabled by infrared-sensitive materials,covering challenges in materials development and device micro-/*** for scaling these techniques across fabrication,performance,and integration are presented,alongside perspectives on potential applications in medication and health,biomimetic vision,and neuromorphic sensory systems,etc.

关键词： flexible infrared sensor infrared-sensitive materials device architectures fabrication techniques design strategies

Unraveling the exceptional kinetics of Zn‖organic batteries in hydrated deep eutectic solution

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Journal of Energy Chemistry 2025年第2期101卷 570-577页

作者： duo Chen Yuanhang Wang Tengyu Yao Hang Yang Laifa Shen Jiangsu Key Laboratory of Electrochemical Energy Storage Technologies College of Materials Science and Technology Nanjing University of Aeronautics and Astronautics Christopher Ingold Laboratory Department of Chemistry University College London

Intuitively,the solvation structure featuring stronger interacted sheath in deep eutectic solution（DES）electrolyte would result in sluggish interfacial charge transfer and intense polarization,which obstructs its practical application in emerging Zn based ***,here we discover a Zn‖organic battery with exceptional kinetics properties enabled by a hydrated DES electrolyte,which can render higher discharge capacity,smaller voltage polarization,and faster kinetics of charge transfer in comparison with conventional aqueous 3 M ZnCl2electrolyte,though its viscosity is two orders of magnitude higher than the *** improved kinetics of charge transfer and ion diffusion is demonstrated to originate from the local electron structure regulation of cathode in hydrated DES ***,the DES electrolyte has also been shown to restrict parasitic reaction associated with active water by preferential urea-molecular adsorption on Zn surface and stronger water trapping in solvation structure,giving rise to long-term stable dendrite-free Zn plating/*** work provides a new rationale for understanding electrochemical behaviors of organic cathodes in DES electrolyte,which is conducive to the development of high-performance Zn‖organic batteries.

关键词： Deep eutectic Dendrite-free Kinetics Zn-based battery

Incidence of hematological toxicity with use of immunotherapy and chemotherapy in advanced non-small cell lung cancer

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中华医学杂志(英文版) 2025年第2期138卷 2024 Sep 19页

作者： Fu Wenhai Jiang Yu Ye Wenjun Zhong ran Xiong Shan Li Jianfu Li Caichen He Jianxing liang Wenhua Department of Thoracic Surgery and Oncology The First Affiliated Hospital of Guangzhou Medical University Guangzhou Guangdong China State Key Laboratory of Respiratory Disease Guangzhou Guangdong China National Clinical Research Center for Respiratory Disease Guangzhou Guangdong China Guangzhou Institute of Respiratory Health Guangzhou Guangdong China

Synthesis of ordered mesoporous crystalline boron phosphate scaffold with double diamond surface structure

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Science China Chemistry 2025年第2期68卷 590-600页

作者： Chao Wang Wen-duo Lu Jiao Wang Shunai Che An-Hui Lu Lu Han School of Chemical Science and Engineering Tongji UniversityShanghai 200092China State Key Laboratory of Fine Chemicals Liaoning Key Laboratory for Catalytic Conversion of Carbon ResourcesSchool of Chemical EngineeringDalian University of TechnologyDalian 116024China School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative MoleculesState Key Laboratory of Metal Matrix CompositesShanghai Key Laboratory for Molecular Engineering of Chiral DrugsShanghai Jiao Tong UniversityShanghai 200240China

Ordered mesoporous materials have received great attention due to their well-defined pore structures and potential applications in catalysis,adsorption,separation,drug delivery,*** various compositions of mesoporous solids have been successfully prepared,the preparation of crystalline non-metallic oxyacid salts with ordered mesoporosity remains a major ***,we report the synthesis of a mesoporous solid acid crystalline boron phosphate(BPO_(4))catalyst with a bicontinuous shifted double diamond(SDD)hyperbolic surface *** BPO_(4) scaffold was obtained by synergistic selfassembly in a mixed solvent of water and tetrahydrofuran using the diblock copolymer poly(ethylene oxide)-block-polystyrene as template and phosphoric acid and orthoboric acid as inorganic *** structure consists of two sets of diamond networks adjacent to each other with a mesostructural scale tetragonal symmetry(space group I4_(1)/amd)with unit cell parameters of a=80 nm and c=113 nm,which affords the scaffold a specific surface area of 44 m^(2)/*** a solid acid catalytic material,the SDD BPO_(4) scaffold exhibited excellent catalytic activity at room temperature with a conversion of furfural to 2-(dimethoxymethyl)furan over 70%and can be reused after recovery without serious loss of *** the propane oxidative dehydrogenation reaction,SDD BPO_(4) demonstrated high olefin productivity and selectivity while maintaining high reaction *** study provides ideas for the preparation of ordered mesoporous crystalline catalytic materials and demonstrates their potential for practical applications.

关键词： boron phosphate mesoporous material self-assembly block copolymer catalyst

Hemispheric asymmetries and network dysfunctions in adolescent depression:A neuroimaging study using resting-state functional magnetic resonance imaging

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World Journal of Psychiatry 2025年第2期15卷 100-108页

作者： Ying Xiong Ren-Qiang Yu Xing-Yu Wang Shun-Si liang Jie ran Xiao Li Yi-Zhi Xu Department of Hematology Chongqing General HospitalChongqing UniversityChongqing 401147China Department of Radiology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Psychiatry The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China

BACKGROUND Currently,adolescent depression is one of the most significant public health concerns,markedly influencing emotional,cognitive,and social *** advancements in distinguish the neurobiological substrates underlying depression,the intricate patterns of disrupted brain network connectivity in adolescents warrant further *** To elucidate the neural correlates of adolescent depression by examining brain network connectivity using resting-state functional magnetic resonance imaging(rs-fMRI).METHODS The study cohort comprised 74 depressed adolescents and 59 healthy controls aged 12 to 17 *** underwent rs-fMRI to evaluate functional connectivity within and across critical brain networks,including the visual,default mode network(DMN),dorsal attention,salience,somatomotor,and frontoparietal control *** Analyses revealed pronounced functional disparities within key neural circuits among adolescents with *** results demonstrated existence of hemispheric asymmetries characterized by enhanced activity in the left visual network,which contrasted the diminished activity in the right *** DMN facilitated increased activity within the left prefrontal cortex and reduced engagement in the right hemisphere,implicating disrupted self-referential and emotional processing ***,an overactive right dorsal attention network and a hypoactive salience network were identified,underscoring significant abnormalities in attentional and emotional regulation in adolescent *** The findings from this study underscore distinct neural connectivity disruptions in adolescent depression,underscoring the critical role of specific neurobiological markers for precise early diagnosis of adolescent *** observed functional asymmetries and network-specific deviations elucidate the complex neurobiological architecture of adolescent depression,supporting the development of targeted the

关键词： Adolescent depression Brain network connectivity Neuroimaging biomarkers Functional magnetic resonance imaging Default mode network Salience network Hemispheric asymmetry

Endogenous/exogenous dual stimulation“ROS engineering”amplification combined with autophagy-augmented for efficient ferroptosis therapy

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Nano Research 2025年第1期18卷 597-606页

作者： Pengye Du Pengpeng Lei Yuan liang ran An Yi Wei Shuyu Liu Jianhao Zheng Hongjie Zhang State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei 230026China Department of Chemistry Tsinghua UniversityBeijing 100084China

“Reactive oxygen species(ROS)engineering”is one of the most promising anti-tumor treatments developed in recent *** explosion of ROS will cause oxidative stress,thereby inducing tumor cell ***,ROS accumulation in tumor cells is eliminated by endogenous cellular self-regulation *** this work,a kind of endogenous/exogenous dual stimulation nanoagent noted as UMZC is *** reaction occurs between NH_(2)-MIL-88B(Fe)contained in UMZC and the overexpression of endogenous H_(2)O_(2)to generate ROS,amplified by endogenous H_(2)S of colon tumor *** addition,NH_(2)-MIL-88B(Fe)also functions to deplete glutathione(GSH)for stopping it from consuming *** nanoparticles at the core of UMZC convert near-infrared into visible light,which excites zinc phthalocyanine to initiate the photochemical reaction that generates more ROS,thus alleviating the lack of tissue penetration depth for visible *** agonist chitosan oligosaccharides induce enhancement of cellular autophagy for disrupting cellular metabolic stress and the resistance to oxidative stress of tumor cells,allowing“ROS engineering”to fully exert anti-tumor *** of ROS generation,GSH depletion,and induced cellular autophagy caused by nanoagents have promoting effects on the occurrence of ***,the nanoagents show the ability to effectively treat colon tumors.

关键词： reactive oxygen species(ROS)engineering upconversion nanoparticles cellular autophagy ferroptosis tumor therapy

Prompt fission neutron uranium logging(Ⅱ):dead-time effect of the neutron time spectrum

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Nuclear Science and Techniques 2025年第2期36卷 82-94页

作者： Yan Zhang Chi Liu Shi-liang Liu Hao-ran Zhang Hai-Tao Wang Jin-Hui Qu Wen-Xing Hu Ren-Bo Wang Bin Tang National Key Laboratory of Uranium Resources Exploration-Mining and Nuclear Remote Sensing East China University of TechnologyNanchang 330013China Fundamental Science on Radioactive Geology and Exploration Technology Laboratory East China University of TechnologyNanchang 330013China

The acquisition of neutron time spectrum data plays a pivotal role in the precise quantification of uranium via prompt fission neutron uranium logging(PFNUL).However,the impact of the detector dead-time effect remains paramount in the accurate acquisition of the neutron time ***,it is imperative for neutron logging instruments to establish a dead-time correction method that is not only uncomplicated but also practical and caters to various logging *** study has formulated an innovative equation for determining dead time and introduced a dead-time correction method for the neutron time spectrum,called the“dual flux method.”Using this approach,a logging instrument captures two neutron time spectra under disparate neutron *** carefully selecting specific“windows”on the neutron time spectrum,the dead time can be accurately *** substantiate its efficacy and discern the influencing factors,experiments were conducted utilizing a deuterium-tritium(D-T)neutron source,a Helium-3(3He)detector,and polyethylene shielding to collate and analyze the neutron time spectrum under varying neutron fluxes(at high voltages).The findings underscore that the“height”and“spacing”of the two windows are the most pivotal influencing ***,the“height”(fd)should surpass 2,and the“spacing”twd should exceed 200μ*** dead time of the 3 He detector determined in the experiment was 7.35μ*** the dead-time correction,the deviation of the decay coefficients from the theoretical values for the neutron time spectrum under varying neutron fluxes decreased from 12.4%to within 5%.Similarly,for the PFNUL instrument,the deviation in the decay coefficients decreased from 22.94 to 0.49%after correcting for the dead-time *** results demonstrate the exceptional efficacy of the proposed method in ensuring precise uranium *** dual flux method was experimentally validated as a universal approach applicable to pulsed neutron logging instrumen

关键词： PFNUL Neutron time spectrum Dead time Pulsed source Correction method

Micro-strain regulation strategy to stabilize perovskite lattice based on the categories and impact of strain on perovskite solar cells

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Journal of Energy Chemistry 2025年第1期100卷 578-604页

作者： Caixia Li Wenwu Liu Shiji Da Lingbin Kong Fen ran State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals Lanzhou University of TechnologyLanzhou 730050GansuChina School of Materials Science and Engineering Lanzhou University of TechnologyLanzhou 730050GansuChina

Photovoltaic metal halide perovskite solar cells(PSCs) convert light to electricity more efficiently than crystalline silicon cells, and the cost of materials used to make them is lower than that of silicon *** efficiency is not a core issue affecting the application of perovskite solar cells in special *** present, stability is the major technical encounters that hinders its further commercial development. Microstrain in PSCs is currently a significant factor responsible for the device's instability. Strain-induced ion migration is widely believed to accelerate perovskite degradation even when external stimuli are ***, it is imperative to study strain to enhance the stability of PSCs. This paper reviews recent developments to understand strain's origin and effect mechanisms on performance of PSCs, including ion migration,failure behavior, defect formation, and its effect on photoelectric properties, stability, and ***, several well-known strain management strategies are systematically introduced based on the strain effect mechanism and strain engineering on the film, providing more clues for further preparation with increased stability. The manipulation of external physical strain applied from films to entire devices has been extensively studied. Furthermore, recommendations for future research directions and chemical approaches have been provided. It is emphasized that strain engineering plays a crucial role in improving the efficiency and longevity of PSCs. Tensile strain causes rapid degradation, while moderate compressive strain and external strain control could improve properties and stability. Efforts should focus on controlling compressive strain to mitigate residual tensile strain and introducing it in a controlled manner. Future research endeavors may focus on exploring these pathways to improve the efficiency and lifespan of PSCs.

关键词： Halide perovskite solar cells Strain origins Strain effects Strain management

Systematic experimental investigation on pressure build-up characteristics of water-jet injection into a molten LBE pool

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Nuclear Science and Techniques 2025年第1期36卷 161-174页

作者： Hao-ran Huang Zi-Jian Deng Song-Bai Cheng Jia-Yue Chen Sino‑French Institute of Nuclear Engineering and Technology Sun Yat-Sen UniversityZhuhai 519082China College of Nuclear Science and Technology Harbin Engineering UniversityHarbin 150001China

In the scenario of a steam generator tube rupture accident in a lead-cooled fast reactor,secondary circuit subcooled water under high pressure is injected into an ordinary-pressure primary vessel,where a molten lead-based alloy(typically pure lead or lead-bismuth eutectic(LBE))is used as the *** clarify the pressure build-up characteristics under water-jet injection,this study conducted several experiments by injecting pressurized water into a molten LBE pool at Sun Yat-sen *** obtain a further understanding,several new experimental parameters were adopted,including the melt temperature,water subcooling,injection pressure,injection duration,and nozzle *** detailed analyses,it was found that the pressure and temperature during the water-melt interaction exhibited a consistent variation trend with our previous water-droplet injection mode LBE ***,the existence of a steam explosion was confirmed,which typically results in a much stronger pressure *** the non-explosion cases,increasing the injection pressure,melt-pool temperature,nozzle diameter,and water subcooling promoted pressure build-up in the melt ***,a limited enhancement effect was observed when increasing the injection duration,which may be owing to the continually rising pressure in the interaction vessel or the isolation effect of the generated steam *** of whether a steam explosion occurred,the calculated mechanical and kinetic energy conversion efficiencies of the melt were relatively small(not exceeding 4.1%and 0.7%,respectively).Moreover,the range of the conversion efficiency was similar to that of previous water-droplet experiments,although the upper limit of the jet mode was slightly lower.

关键词： Lead-cooled fast reactor Steam generator tube rupture accident Pressure build-up characteristics Experimental study Pressure water-jet injection