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Nitrogen application and intercropping change microbial community diversity and physicochemical characteristics in mulberry and alfalfa rhizosphere soil

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Journal of Forestry Research 2021年第5期32卷 2121-2133页

作者： Xiuli Zhang Zhiyuan Teng Huihui Zhang dunjiang cai Jingyun Zhang Fanjuan Meng Guangyu Sun Key Laboratory of Saline-Alkali Vegetation Ecology Restoration(Northeast Forestry University) Ministry of EducationHarbin 150040People’s Republic of China College of Life Science Northeast Forestry UniversityHarbin 150040People’s Republic of China College of Resource&Environment Northeast Agricultural UniversityHarbin 150030People’s Republic of China Institute of Crop Development Heilongjiang Academy of Land Reclamation and Agricultural SciencesJiamusiHeilongjiangPeople’s Republic of China

Intercropping of mulberry(Morus alba L.)and alfalfa(Medicago sativa L.)is a new forestry-grass compound model in China,which can provide high forage yields with high *** application is one of the important factors determining the production and quality of this *** elucidate the advantages of intercropping and nitrogen application,we analyzed the changes of physicochemical properties,enzyme activities,and microbial communities in the rhizosphere *** used principal components analysis(PCA)and redundancy discriminators analysis to clarify the relationships among treatments and between treatments and environmental factors,*** results showed that nitrogen application significantly increased pH value,available nitrogen content,soil water content(SWC),and urea(URE)activity in rhizosphere soil of monoculture *** contrast,intercropping and intercropping+N significantly decreased pH and SWC in mulberry ***,intercropping and intercropping+N sharply reduced soil organic matter content and SWC in alfalfa ***,intercropping,and intercropping+N increased the values of McIntosh diversity(U),Simpson diversity(D),and Shannon-Weaver diversity(H’)in mulberry ***,PC A scatter plots showed clustering of monoculture mulberry with nitrogen(MNE)and intercropping mulberry without nitrogen(M0).Intercropping reduced both H’and D but nitrogen application showed no effect on diversity of microbial communities in *** were obvious differences in using the six types of carbon sources between mulberry and alfalfa *** and intercropping increased the numbers of sole carbon substrate in mulberry treatments where the relative use rate exceeded 4%.While the numbers declined in alfalfa with nitrogen and *** indicated that URE was positive when intercropping mulberry was treated with nitrogen,but was negative in monoculture alfalfa treated with *** pH and SWC were posi

关键词： Mulberry intercropped with alfalfa Nitrogen application Principal components analysis Redundancy discriminators analysis Rhizosphere soil

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高寒区苜蓿越冬的生理适应性

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东北林业大学学报 2005年第6期33卷 49-51页

作者：孙广玉李威蔡敦江王学臣东北林业大学哈尔滨150040 黑龙江省农垦科学院作物所中国农业大学

为了研究不同苜蓿品种的抗寒能力,测定了不同品种的苜蓿根部不同时间中可溶性糖、脯氨酸、游离氨基酸和丙二醛含量。结果表明:不同时期肇东苜蓿、准葛尔和CW200品种根部的可溶性糖、脯氨酸、游离氨基酸和丙二醛含量表现不同,品种和不同... 详细信息

为了研究不同苜蓿品种的抗寒能力,测定了不同品种的苜蓿根部不同时间中可溶性糖、脯氨酸、游离氨基酸和丙二醛含量。结果表明:不同时期肇东苜蓿、准葛尔和CW200品种根部的可溶性糖、脯氨酸、游离氨基酸和丙二醛含量表现不同,品种和不同时期之间存在显著性差异。品种之间比较,准格尔苜蓿的可溶性糖、脯氨酸、游离氨基酸积累量明显高于其它两个品种,CW200根部中可溶性糖、脯氨酸和游离氨基酸的积累比其它两个品种低。3个品种的丙二醛含量在温度降低初期含量均较高,但随气温的下降其含量降低,并保持相对稳定;准格尔苜蓿丙二醛含量下降较为明显,并且随温度降低丙二醛含量比其他两个品种低,CW200丙二醛含量高于其它两个品种。综合测定的参数说明,准格尔苜蓿抗寒能力较强,CW200较差。

关键词：苜蓿抗寒性游离脯氨酸丙二醛

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黑龙江垦区苜蓿机械化生产技术的研究

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中国草地 1998年第2期20卷 25-28,63页

作者：周兴民蔡敦江朱廉冯元琳杨丹霞王继红黑龙江省农垦科学院

研究形成了一套黑龙江垦区苜蓿机械生产技术，包括７个优良品种、４种建植方式、１７种适用除草剂、２份高效根瘤菌种、持续高产优质的适宜割期、防腐保质的收贮工艺与配套的半干青贮及添加剂青贮技术、种子田生产和隙地种草技术，获效... 详细信息

研究形成了一套黑龙江垦区苜蓿机械生产技术，包括７个优良品种、４种建植方式、１７种适用除草剂、２份高效根瘤菌种、持续高产优质的适宜割期、防腐保质的收贮工艺与配套的半干青贮及添加剂青贮技术、种子田生产和隙地种草技术，获效益５８３．４万元。

关键词：苜蓿机械化生产优良品种栽培方式根瘤菌

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Spread of *** Aerosols Carrying Antibiotic-Resistant Genes （Including MRSA） from Chicken Farms to Their Neighborhood Residents Environment

Spread of *** Aerosols Carrying Antibiotic-Resistant Genes ...

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2012年环境污染与大众健康学术会议

作者： dunjiang Liu 1 Tongjie Chai 1, * Yumei cai 1 Lingyu Sun 1 Haiyu Hao 1 Jian Wang 2 1 College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonoses of Animal Origin of Shandong Province, Tai’an, 271018, China State Environmental Protection Key Laboratory of Microorganism Application and Risk Control, Tsinghua University, 100084 Beijing, China. 2 Bureau of Animal Husbandry and Veterinary Zouping County, Shandong Province, China.

There is a rather limited understanding concerning the antibiotic-resistance of the airborne S. aureus and the transmission of the antibiotic-resistant genes it carries Therefore, we isolated 149 S. aureus strains from the samples collected from the feces, the indoor air and the outdoor air of 6 chicken farms, and performed the research on them with 15 types of antibiotics and the REP-PCR trace identification. The 100% homologous strains were selected to conduct the research on the carrying and transmission status of the antibiotic-resistant genes. The results revealed that 5.37% strains（8/149） were resistant to methicillins （MRSA）, and 94% strains （140/149） were resistant to compound sulfamethoxazole, etc. In addition, these strains displayed a resistance to multiple antibiotics （4, 5 or 6 types） and there were also 3 strains resistant to 9 antibiotics. the strains with the same REP-PCR trace identification result carried the same type of antibiotic-resistant genes. The results showed that airborne transmission not only causes the spread of epidemic diseases but also exerts threats to the public health of a community.

关键词： chicken farming environment aerosols of Staphylococcus aureus including MRSA REP-PCR tracing antibiotic-resistance antibiotic-resistant genes significance to epidemiology and public health.

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