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Current Zoology 2015年第3期61卷 397-411页

作者： Jacqueline CODRON Kevin J duffy Nico L AVENANT matt SPONHEIMER Jennifer LEICHLITER Oliver PAINE Paul SANDBERG Daryl CODRON Institute of Systems Science Durban University of Technology Durban South Africa Karoo Palaeontology Deparlnaent National Museum PO Box 266 Bloemfontein 9301 RSA School of mathematics Statistics and Computer Science University of KwaZulu-Natal Private Bag X01 Scottsville 3209 RSA Denartment of mammalogv. National Museum. PO Box 266. Bloemfontein 9301. RSA Department of Anthropology University of Colorado at Boulder Boulder CO80309 USA Florisbad Quaternary Research Department National Museum PO Box 266 Bloemfontein 9301 RSA

Species＇ partitioning of resources remains one of the most integral components for understanding community assem- bly. Analysis of stable carbon and nitrogen isotopes in animal tissues has the potential to help resolve patterns of partitioning be- cause these proxies represent the individual＇s diet and trophic niche, respectively. Using free-ranging rodents in a southern Afri- can savanna as a model community, we find that syntopic species within habitats occupy distinct isotope niches. Moreover, spe- cies with strongly overlapping isotope niches did not overlap in their spatial distribution patterns, suggesting an underlying effect of competitive exclusion. Niche conservatism appears to characterize the behaviour of most species in our sample - with little or no observed changes across habitats - with the exception of one species, mastomys coucha. This species displayed a generalist distribution, being found in similar abundances across a variety of habitats. This spatial pattern was coupled with a generalist isotope niche that shifted across habitats, likely in response to changes in species composition over the same spatial gradient. The case for M. coucha supports contentions that past competition effects played a significant evolutionary role in shaping community structures of today, including the absence of strong interspecific niche overlaps within particular habitats. Our study highlights the value of stable isotope approaches to help resolve key questions in community ecology, and moreover introduces novel ana- lytical approaches to quantifying isotope niche breadths and niche overlaps that are easily comparable with traditional metrices

关键词： Competition Diet Niche breadth Niche overlap Sterkfontein Valley

Spatiotemporal event detection: a review

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International Journal of Digital Earth 2020年第12期13卷 1339-1365页

作者： manzhu Yu Myra Bambacus Guido Cervone Keith Clarke Daniel duffy Qunying Huang Jing Li Wenwen Li Zhenlong Li Qian Liu Bernd Resch Jingchao Yang Chaowei Yang Department of Geography Pennsylvania State UniversityUniversity ParkPAUSA NASA Goddard Space Flight Center NASA Goddard Space Flight CenterGreenbeltMDUSA Department of Geography University of California-Santa BarbaraSanta BarbaraCAUSA Department of Geography University of Wisconsin-MadisonMadisonWIUSA Department of Geography and the Environment University of DenverDenverCOUSA School of Geographical Sciences and Urban Planning Arizona State UniversityTempeAZUSA Department of Geography University of South CarolinaColumbiaSCUSA NSF Spatiotemporal Innovation Center&Dept.of Geography and GeoInformation Science George Mason UniversityFairfaxVAUSA Department of Geoinformatics University of SalzburgKapitelgasseSalzburgAustria Center for Geographic Analysis Harvard UniversityBostonMAUSA

The advancements of sensing technologies,including remote sensing,in situ sensing,social sensing,and health sensing,have tremendously improved our capability to observe and record natural and social phenomena,such as natural disasters,presidential elections,and infectious *** observations have provided an unprecedented opportunity to better understand and respond to the spatiotemporal dynamics of the environment,urban settings,health and disease propagation,business decisions,and crisis and *** event detection serves as a gateway to enable a better understanding by detecting events that represent the abnormal status of relevant *** paper reviews the literature for different sensing capabilities,spatiotemporal event extraction methods,and categories of applications for the detected *** novelty of this review is to revisit the definition and requirements of event detection and to layout the overall workflow(from sensing and event extraction methods to the operations and decision-supporting processes based on the extracted events)as an agenda for future event detection *** is presented on the current challenges to this research agenda,and future directions are discussed for conducting spatiotemporal event detection in the era of big data,advanced sensing,and artificial intelligence.

关键词： GeoAI geography and geoscience human dynamics digital earth computational challenges cloud computing internet of things

Methods for extracellular vesicle isolation from cancer cells

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Cancer Drug Resistance 2020年第3期3卷 371-384页

作者： Israa Salem Nicole M.Naranjo Amrita Singh Rachel DeRita Shiv Ram Krishn Luca S.Sirman Fabio Quaglia Alexander duffy Nicholas Bowler Aejaz Sayeed Lucia R.Languino Department of Cancer Biology Sidney Kimmel Cancer CenterThomas Jefferson UniversityPhiladelphiaPA 19107USA. Astellas Institute for Regenerative Medicine MarlboroughMA 01752USA. School of Veterinary Medicine University of PennsylvaniaPhiladelphiaPA 19104USA. Baruch S.Blumberg Institute DoylestownPA 18902USA.

Cells are known to release different types of vesicles such as small extracellular vesicles(sEVs)and large extracellular vesicles(LEVs).sEVs and LEVs play important roles in intercellular communication,pre-metastatic niche formation,and disease progression;both can be detected cell culture media and biological *** and LEVs contain a variety of protein and RNA cargo,and they are believed to impact many biological functions of the recipient cells upon their internalization or binding to cell surface *** has recently been established that standard isolation techniques,such as differential ultracentrifugation,yield a mixed population of ***,density gradient ultracentrifugation has been reported to allow the isolation of sEVs without cellular ***,we describe the most common methods used to isolate sEVs from cell culture medium,mouse and human plasma,and a new technique for isolating sEVs from tissues as *** article also provides detailed procedures to isolate LEVs.

关键词： Extracellular vesicles differential ultracentrifugation iodixanol density gradient cell culture medium plasma tissue

南京汉族人群duffy血型等位基因FYES及FYX调查研究

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国际检验医学杂志 2017年第16期38卷 2205-2206,2209页

作者：马玲刘衍春江苏省血液中心输血研究室江苏南京210042

目的研究南京地区汉族人群中duffy血型等位基因FYES及FYX的表达情况。方法随机提取200例无偿献血者EDTA-K2抗凝外周血血样DNA,采用PCR-SSP方法进行duffy血型FYA、FYB等位基因分型;采用Sanger双脱氧链末端终止测序法分析duffy血型FYES及... 详细信息

目的研究南京地区汉族人群中duffy血型等位基因FYES及FYX的表达情况。方法随机提取200例无偿献血者EDTA-K2抗凝外周血血样DNA,采用PCR-SSP方法进行duffy血型FYA、FYB等位基因分型;采用Sanger双脱氧链末端终止测序法分析duffy血型FYES及FYX等位基因序列特点。结果PCR基因分型结果与测序结果一致,200例标本中,检出FYA/FYA 180例,FYA/FYB 20例;测序结果表明,200例标本中未发现FYES及FYX等位基因表达。结论 FYES及FYX等位基因在中国南京地区汉族人群中表达频率极低,与其他人种相比具有显著性差异。

关键词： duffy血型基因基因频率 FYES FYX

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红细胞意外抗体鉴定在输血安全中的重要作用

中国实用医药 2012年第32期7卷 57-58页

作者：马宏伟谢辉河南省红十字血液中心血型研究室郑州450012 郑州人民医院检验科

目的检测分析患者血清中红细胞意外抗体的特异性和分布。方法使用盐水介质法、聚乙二醇间接抗球蛋白法和微柱凝胶法对患者血清进行意外抗体特异性鉴定。结果 149例患者标本中,57例仅存在自身抗体,88例仅存在同种抗体,4例同时存在自身抗... 详细信息

目的检测分析患者血清中红细胞意外抗体的特异性和分布。方法使用盐水介质法、聚乙二醇间接抗球蛋白法和微柱凝胶法对患者血清进行意外抗体特异性鉴定。结果 149例患者标本中,57例仅存在自身抗体,88例仅存在同种抗体,4例同时存在自身抗体和同种抗体。同种抗体包括5个血型系统,分别为Rh系统(77.1%)、MNS系统(16.3%)、Lewis系统(3.3%)、P系统(2.2%)和Duf-fy系统(1.1%)。Rh系统抗体分布为:抗-E(39.4%)、抗-cE(29.7%)、抗-D(12.7%)、抗-Ce(5.6%)、抗-DC(4.2%)、抗-C(4.2%)、抗-e(2.8%)和抗-c(1.4%)。结论意外抗体的鉴定有助于患者获得相容性血液,保障临床输血安全有效。

关键词：血型意外抗体抗体鉴定输血安全

抗-E及抗-Ec合并抗-Fyb致新生儿溶血症二例及文献复习

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天津医药 2018年第6期46卷 654-656页

作者：石武娟穆郁宋立张芳麻庆荣王丹天津市儿童医院新生儿科 300134

新生儿溶血病(HDN)在妊娠早期可引起流产、早产、胎儿水肿、胎儿宫内贫血甚至胎儿死亡;新生儿可出现黄疸、贫血、肝脾肿大、水肿、核黄疸后遗症等。本文回顾了抗-E及抗-Ec合并抗-Fyb致新生儿溶血症2例患者的诊疗经过,并复习了该病流行... 详细信息

新生儿溶血病(HDN)在妊娠早期可引起流产、早产、胎儿水肿、胎儿宫内贫血甚至胎儿死亡;新生儿可出现黄疸、贫血、肝脾肿大、水肿、核黄疸后遗症等。本文回顾了抗-E及抗-Ec合并抗-Fyb致新生儿溶血症2例患者的诊疗经过,并复习了该病流行病学情况、诊断及治疗的相关文献资料,以提高对本病的认识。

关键词：贫血溶血性免疫性 Rh-Hr血型系统 duffy血型系统重症监护新生儿

中国南京地区十个红细胞血型系统稀有抗原的大规模调查

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中国实验血液学杂志 2011年第1期19卷 231-234页

作者：马玲刘衍春薛敏魏鹏唐荣才江苏省血液中心输血研究室江苏南京210042

本研究调查南京地区人群中稀有血型抗原的类型及分布情况,为稀有血型病人的临床用血和献血招募提供基础性资料。采用4M尿素对红细胞进行Kidd血型系统的Jk(a-b-)表型筛选;利用抗血清对H系统的H-、MNS系统的GPA-、Gerbich系统的GPC-、Ii... 详细信息

本研究调查南京地区人群中稀有血型抗原的类型及分布情况,为稀有血型病人的临床用血和献血招募提供基础性资料。采用4M尿素对红细胞进行Kidd血型系统的Jk(a-b-)表型筛选;利用抗血清对H系统的H-、MNS系统的GPA-、Gerbich系统的GPC-、Ii系统的i+、Lutheran系统的Lub-表型进行筛选;对Kell系统的k-和Jsb-、duffy系统的Fya-、Ok系统的Ok-、MNS系统的s-和Digeo系统的Dib-表型采用多重PCR的方法进行筛选。结果表明:在40337例标本中,筛选出2例Jk(a-b-)表型;在1 782例标本中,筛出Fy(a-b+)表型3例,尚未发现Fy(a-b-)的表型,也未发现Ok-、s-和Dib-表型。在2 002、2 003、2 084、10 189和10 004例标本中,未发现相应的GPA-、GPC-、Lub-、H-和i+稀有表型者。在2017例标本中,也未发现k-和Jsb-的表型存在。结论:南京地区人群中Jk(a-b-)表型频率为0.0049%,Fy(a-b+)表型频率为0.168%,在现有筛查人数中尚未发现所查其它血型系统稀有抗原的存在。

关键词：稀有血型血型筛查红细胞南京地区

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新生儿溶血病意外抗体检测分析

中国当代医药 2012年第33期19卷 78-79页

作者：马宏伟刘旭华河南省红十字血液中心血型研究室河南郑州450012 郑州人民医院检验科河南郑州450003

目的调查研究导致新生儿溶血病的意外抗体分布情况,为诊断和治疗提供支持。方法按照《全国临床检验操作规程》对573例病理性黄疸患儿标本进行溶血三项试验和血清胆红素测定,同时进行意外抗体筛选,阳性标本采用经典抗球蛋白法鉴定抗体特... 详细信息

目的调查研究导致新生儿溶血病的意外抗体分布情况,为诊断和治疗提供支持。方法按照《全国临床检验操作规程》对573例病理性黄疸患儿标本进行溶血三项试验和血清胆红素测定,同时进行意外抗体筛选,阳性标本采用经典抗球蛋白法鉴定抗体特异性。结果共检出意外抗体阳性标本38例(6.63%),其中Rh系统35例(92.1%)、MNS系统2例(5.3%)、duffy系统1例(2.6%)。结论意外抗体的筛查和鉴定有助于新生儿溶血病的及时诊断和治疗,对提高生育质量具有重要意义。

关键词：新生儿溶血病血型意外抗体检测分析

Landau-Kleffner综合征中双侧颞上区的容量减小

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世界核心医学期刊文摘（神经病学分册） 2005年第3期1卷 57-57页

作者： Takeoka M. Riviello Jr. J.J. duffy F.H. 高方 Div. Epilepsy Clin. Neurophysiol. Department of Neurology Ch ildrens Hospital 300 Longwood Ave. Boston MA 02115 United States Dr.

No specific anatomic abnormalities have been detected in typical Landau Kleff ner syndrome (LKS), an acquired epileptic aphasia with language regression in ch ildren. In four children with typical LKS without obvious anatomic abnormalities , the authors performed MRI volumetric analysis of various neocortical regions a nd subcortical substructures. Volume reduction was detected in bilateral superio r temporal areas (26 to 51%), specifically in planum temporale (25 to 63%) and superior temporal gyrus (25 to 57%), where receptive language is localized.

关键词： Landau 皮质区颞平面颞上回儿童语言