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L-缬氨酸合成的代谢流量分析

生物工程学报 2004年第3期20卷 403-407页

作者：李秀敏李乃强杨毅姜绪林邱勇隽张星元江南大学工业生物技术教育部重点实验室无锡214036 上海凯赛研发中心有限公司上海201203

分别测定谷氨酸棒杆菌 (Corynebacteriumglutamicum)AS1 4 95及其 3个逐个叠加不同遗传标记的突变株AA36 1、AAT2 31和AATV341在特定培养时段 (2 6～ 2 8h)L 缬氨酸等代谢物的胞外浓度 ,由此计算这一时段这些代谢物在发酵液中积累 (或消... 详细信息

分别测定谷氨酸棒杆菌 (Corynebacteriumglutamicum)AS1 4 95及其 3个逐个叠加不同遗传标记的突变株AA36 1、AAT2 31和AATV341在特定培养时段 (2 6～ 2 8h)L 缬氨酸等代谢物的胞外浓度 ,由此计算这一时段这些代谢物在发酵液中积累 (或消耗 )的速率 ,分别做出这 4株菌在拟稳态下的代谢流量分布图 ,进而研究育种过程中不同遗传标记的叠加对代谢网络中L 缬氨酸合成流量分布的影响。结果表明遗传标记的引入使流量分配发生了重大变化 ,节点处的流量分配朝着有利于L 缬氨酸合成的方向改变。 6 磷酸葡萄糖节点处流入EMP途径和HMP途径的流量分配由 17 0∶83 0变为 2 4 3∶75 7;丙酮酸节点处流入L 缬氨酸合成途径和其他途径的流量分配由 15 8∶84 2变为 76 7∶2 3 3。L 缬氨酸合成的分支途径上的流量由最初的 5 37增大为 37 3,乳酸合成途径的流量从 11 1最后降为 1 16 ,L 缬氨酸产量由 4g L提高到 2 4 5g L。代谢流量分布的变化趋势与L 缬氨酸产量的变化趋势是互相吻合的。以 2 噻唑丙氨酸抗性突变 (2 TAr)和L 天冬氨酸氧肟酸盐超敏性突变 (L AAHss)有效地进行代谢流遗传导向的事实 ,在代谢流量分析的层面上 ,证明结构类似物抗性突变和结构类似物超敏性突变是代谢流导向和设计育种的十分有效的手段。

关键词： L-缬氨酸谷氨酸棒杆菌代谢流量分析

Feeding of Riptortus pedestris on soybean plants, the primary cause of soybean staygreen syndrome in the Huang-Huai-Hai river basin

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The Crop Journal 2019年第3期7卷 360-367页

作者： Kai Li Xinxin Zhang Jianqiu Guo hannah Penn Tingting Wu lin Li Hong Jiang Lidan Chang Cunxiang Wu Tianfu Han Soybean Research Institute Nanjing Agricultural UniversityNanjing 210095JiangsuChina MOA Key Laboratory of Soybean Biology (Beijing) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Luoyang Academy of Agriculture and Forestry Sciences Luoyang 471022HenanChina Department of Entomology Louisiana State UniversityLA 70803USA

Staygreen syndrome or Zhengqing in soybean has recently become a major issue for Chinese growers in the Huang-Huai-Hai river *** previous studies revealed that staygreen can be induced when pods/seeds are damaged, it is unknown whether virus infection or insect infestation causes *** determine whether viral infection causes staygreen, a survey of soybean staygreen incidence in the Huang-Huai-Hai river basin was conducted in 2.16 and *** samples were collected and analyzed using DAS-ELISA for Soybean mosaic virus, Watermelon mosaic virus, Bean pod mottle virus, Cucumber mosaic virus, and Bean common mosaic *** survey showed that the severity of soybean staygreen syndrome was most prevalent in Beijing, Henan, Shaanxi, and some parts of Shandong provinces, with yield losses from 0 to nearly 100%, but only a small fraction of samples were positive for the tested viruses.A field cage experiment and an insecticide treatment field trial were conducted to determine the contribution of the bean bug, Riptortus pedestris, to staygreen *** field cage experiment showed that *** treatment resulted in shorter plants, more empty pods, increased numbers of abnormal seeds, and decreased *** field experiment showed that there were fewer *** and less soybean staygreen incidence in fields treated with insecticide than in untreated control ***, these results suggest that *** infestation rather than virus infection induces staygreen syndrome and that growers in this region can mitigate staygreen syndrome via bean bug control.

关键词： Soybean Staygreen syndrome Zhengqing DAS-ELISA Viral infection Bean bug (R. pedestris)

A ＂Triple Trouble＂ Case of Facioscapulohumeral Muscular Dystrophy Accompanied by Peripheral Neuropathy and Myoclonic Epilepsy

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Chinese Medical Journal 2018年第18期131卷 2164-2171页

作者： Xiao-Dan lin Jun-Jie He Feng lin Hai-Zhu Chen Liu-Qing Xu Wei Hu nai-Qing Cai Min-Ting lin Ning wang Zhi-Qiang wang Guo-Rong Xu Department of Neurology and Institute of Neurology First Affiliated Hospital Fujian Medical University Fuzhou Fujian 350005 China Fujian Key Laboratory of Molecular Neurology Fuzhou Fujian 350005 China

Background： Facioscapulohumeral muscular dystrophy （FSHD） is characterized by asymmetric muscular deficit of facial, shoulder-girdle muscles, and descending to lower limb muscles, but it exists in several extramuscular manifestations or overlapping syndromes. Herein, we report a ＂complex disease plus＂ patient with FSHD1, accompanied by peripheral neuropathy and myoclonic epilepsy. Methods： Standard clinical assessments, particular auxiliary examination, histological analysis, and molecular analysis were performed through the new Comprehensive Clinical Evaluation Form, pulsed-field gel electrophoresis-based Southern blot, Multiplex Ligation-dependent Probe Amplification （MLPA）, whole exome sequencing （WES）, and targeted methylation sequencing. Results： The patient presented with mild facial weakness, humeral poly-hill sign, scapular winging, peroneal weakness, drop foot, pes cavus, and myoclonic epilepsy. Furthermore, electrophysiology revealed severely demyelinated and axonal injury. The muscle and nerve biopsy revealed broadly fiber Type II grouping atrophy and myelinated nerve fibers that significantly decreased with thin myelinated fibers and onion bulbs changes. Generalized sharp and sharp-slow wave complexes on electroencephalography support the diagnosis toward myoclonic epilepsy. In addition, molecular testing demonstrated a co-segregated 20-kb 4q35-EcoRI fragment and permissive allele A, which corresponded with D4Z4 hypomethylation status in the family. Both the patient＇s mother and brother only presented the typical FSHD but lacked overlapping syndromes. However, no mutations for hereditary peripheral neuropathy and myoclonic epilepsy were discovered by MLPA and WES. Conclusions： The present study described a ＂tripe trouble＂ with FSHD, peripheral neuropathy, and myoclonic epilepsy, adding the spectrum of overlapping syndromes and contributing to the credible diagnosis of atypical phenotype. It would provide a direct clue on medical care and genetic counseli

关键词： Facioscapulohumeral Muscular Dystrophy Myoclonic Epilepsy Overlapping Syndromes Peripheral Neuropathy TripleTrouble

Comparative analysis of dideoxy sequencing,the KRAS StripAssay and pyrosequencing for detection of KRAS mutation

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World Journal of Gastroenterology 2010年第38期16卷 4858-4864页

作者： Jing Gao Yan-Yan Li Ping-nai Sun lin Shen Key Laboratory of Carc inogenesis and Transla tional Resear ch (Ministry of Education) Department of GI OncologyPeking University School of OncologyBeijing Cancer Hospital and InstituteBeijing 100142China

AIM:To compare the differences between dideoxy sequencing/KRAS StripAssay/pyrosequencing for detection of KRAS mutation in Chinese colorectal cancer (CRC) ***:Formalin-f ixed, paraff in-embedded (FFPE) samples with tumor cells ≥ 50% were collected from 100 Chinese CRC patients at Beijing Cancer Hospital. After the extraction of genome DNA from FFPE samples, fragments contained codons 12 and 13 of KRAS exon 2 were amplified by polymerase chain reaction and analyzed by dideoxy sequencing, the KRAS Strip Assay and pyrosequencing. In addition, the sensitivities of the 3 methods were compared on serial dilutions (contents of mutant DNA: 100%,50%,20%, 5%,10%, 5%,1%,0%) of A549 cell line DNA (carrying the codon 12 Gly>Ser mutation) into wild-type DNA (human normal intestinal mucosa). The results of dideoxy sequencing,the KRAS StripAssay and pyrosequencing were analyzed by Chromas Software, Collector forKRAS Strip Assay and the pyrosequencing PyroMarkTM Q24 system, ***: Among 100 patients, KRAS mutations were identif ied in 34%, 37% and 37% of patients by dideoxy sequencing, the KRAS StripAssay and pyrosequencing, respectively. The sensitivity was highest with the KRAS Strip Assay (1%), followed by pyrosequencing (5%), and dideoxy sequencing was lowest (15%). Six different mutation types were found in this study with 3 main mutations Gly12 Asp (GGT>GAT), Gly12 Val (GGT>GTT) and Gly13 Asp (GGC>GAC). Thirty-three patients were identifi ed to have KRAS mutations by the 3 methods, and a total of 8 patients had conflicting results between 3 methods: 4 mutations not detected by dideoxy sequencing and the KRAS StripAssay were identified by pyrosequencing; 3 mutations not detected by dideoxy sequencing and pyrosequencing were identif ied by the KRAS StripAssay; and 1 mutation not detected by pyrosequencing was conf irmed by dideoxy sequencing and the KRAS StripAssay. Among these discordant results, the results identif ied by dideoxy sequencing were con

关键词： DNA mutational analysis KRAS Mutation Dideoxy sequencing KRAS StripAssay Pyrosequencing

Alloying behavior and characterization of(CoCrFeNiMn)_(90)M_(10)(M=Al,Hf)high-entropy materials fabricated by mechanical alloying

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Transactions of Nonferrous Metals Society of china 2022年第7期32卷 2253-2265页

作者： nai-ran wang Shou-ren wang Xiao-xiang GOU Ze-cheng SHI Jian-xiang lin Guo-qiang LIU Yan wang school of Materials Science and Engineering University of JinanJinan 250022China school of Mechanical Engineering University of JinanJinan 250022China

The alloying behavior and microstructures of the(CoCrFeNiMn)_(90)M_(10)(M=Al,Hf)high-entropy alloy(HEA)powders fabricated by mechanical alloying were *** CoCrFeNiMn)_(90)Al_(10) powders have duplex solid-solution *** contrast,nanocrystalline HfNi_(3) anchoring in amorphous structures is found in the(CoCrFeNiMn)_(90)Hf_(10) ***(CoCrFeNiMn)_(90)Al_(10) powders show better ferromagnetic behaviors,mainly explained by the facilitated motion of the magnetic domain induced by the coherent interface between duplex *** with our previous work,the rules of forming solid-solution and amorphous phase in as-milled HEA powders are preliminarily *** is found that,compared with the as-cast HEA reported previously,the variation range of mixing enthalpy with atomic size difference of the solid-solution formed in as-milled HEA powders is ***,the variation ranges between mixing enthalpy and entropy with atomic size difference of the amorphous phase in HEA powder become wider than those of high-entropy bulk metallic glass.

关键词： high-entropy powder mechanical alloying ferromagnetic properties alloy design phase stability

Translational Regulation of Plant Response to High Temperature by a dual-Function tRNAHls Guanylyltransferase in Rice

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Molecular Plant 2019年第8期12卷 1123-1142页

作者： Ke Chen Tao Guo Xin-Min Li Yi-Min Zhang Yi-Bing Yang wang-Wei Ye nai-Qian Dong Chuan-lin Shi Yi Kan You-Huang Xiang Hai Zhang Ya-Chao Li Ji-Ping Gao Xuehui Huang Qiang Zhao Bin Han Jun-Xiang Shan Hong-Xuan lin National Key Laboratory of Plant Molecular Genetics CAS Centre for Excellence in Molecular Plant Sciences and Collaborative Innovation Center of Genetics & DevelopmentShanghai Institute of Plant Physiology & EcologyShanghai Institute for Biological SciencesChinese Academic of SciencesShanghai 200032China university of the Chinese Academy of Sciences Beijing 100049China school of Life Science and Technology ShanghaiTech UniversityShanghai 201210China National Center for Gene Research CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200233China College of Life and Environmental Sciences Shanghai Normal UniversityShanghai 200234China

As sessile organisms,plants have evolved numerous strategies to acclimate to changes in environmental ***,the molecular basis of this acclimation remains largely *** this study we identified a tRNAHis guanylyltransferase,AET1,which contributes to the modification of pre-tRNAH,s and is required for normal growth under high-temperature conditions in ***,AET1 possibly interacts with both RACK1A and elF3h in the endoplasmic ***,AET1 can directly bind to OsARF mRNAs including the uORFs of OsARF19 and OsARF23,indicating that AET1 is associated with translation ***,polysome profiling assays suggest that the translational status remains unaffected in the aet1 mutant,but that the translational efficiency of OsARF19 and OsARF23 is reduced;moreover,OsARF23 protein levels are obviously decreased in the aet1 mutant under high temperature,implying that AET1 regulates auxin signaling in response to high *** provide new insights into the molecular mechanisms whereby AET1 regulates the environmental temperature response in rice by playing a dual role in tRNA modification and translational control.

关键词： rice translation regulation tRNA modification auxin signaling environmental temperature

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活血化瘀中药注射剂临床应用的调查及干预

中国新药与临床杂志 2010年第4期29卷 315-318页

作者：邵华刘乃丰徐厚明厉伟兰蔡琳玲谢菡东南大学附属中大医院临床药理室江苏南京210009 东南大学附属中大医院心脏科江苏南京210009 东南大学心血管病研究所江苏南京210009 江苏省药品不良反应监测中心江苏南京210002

目的调查活血化瘀中药注射剂临床使用现状,促进其合理使用。方法查阅特定时间内某医院神经科所有住院患者病历和医嘱,详细记录患者诊断、活血化瘀中药注射剂使用情况、脑梗死患者住院时间、住院费用及出院时疗效评价,针对存在问题制定... 详细信息

目的调查活血化瘀中药注射剂临床使用现状,促进其合理使用。方法查阅特定时间内某医院神经科所有住院患者病历和医嘱,详细记录患者诊断、活血化瘀中药注射剂使用情况、脑梗死患者住院时间、住院费用及出院时疗效评价,针对存在问题制定干预措施,比较干预前后药品临床应用情况及对病情的影响。结果干预前81.1%的患者接受了活血化瘀中药注射剂治疗,干预后降为73.8%(P1);干预前使用9种活血化瘀中药注射剂,干预后品种数减少到5种,干预前后分别有72.5%和14.2%患者先后使用2种以上(包括2种)活血化瘀中药注射剂(P1);分别有64.9%和5.5%联用2种以上(包括2种)活血化瘀中药注射剂(P1)。干预前后2组患者血液流变学指标(全血黏度、红细胞比容和红细胞变形指数)均无显著差异(P>0.05),单纯诊断脑梗死患者干预后平均住院日数减少2 d,费用节省近2 000元;脑梗死合并高血压的患者干预后平均住院日数减少1.2 d,费用节省400元,但是干预前后的差异均不显著(P>0.05)。无论脑梗死患者还是脑梗死合并高血压患者干预前后均100%治愈或好转。结论综合干预措施在一定程度上促进了活血化瘀中药注射剂在临床的合理使用。

关键词：活血祛瘀剂中草药脑血管障碍药物不良反应

A transient fiuid–structure interaction analysis strategy and validation of a pressurized reactor with regard to loss-ofcoolant accidents

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Nuclear Science and Techniques 2020年第6期31卷 11-21页

作者： Ying-Chao Ma Xie-lin Zhao Xian-Hui Ye nai-Bin Jiang Jin-Xiong Zhou State Key Laboratory for Strength and Vibration of Mechanical Structures School of AerospaceXi’an Jiaotong UniversityXi’an 710049China Key Laboratory of Nuclear Reactor System Design Technology Nuclear Power Institute of ChinaChengdu 610200China Sino-French Institute of Nuclear Engineering and Technology Sun Yat-Sen UniversityZhuhai 519082China

A loss-of-coolant accident(LOCA)is one of the basic design considerations for nuclear reactor safety analysis.A LOCA induces propagation of a depressurization wave in the coolant,exerting hydrodynamic forces on structures viafiuid–structure interaction(FSI).The analysis of hydrodynamic forces on the core structures during a LOCA process is *** describe the implementation of a numerical strategy for prestressed *** consists of an initialization and a restarted transient analysis process,all implemented via the ANSYS Workbench by system coupling of ANSYS and *** strategy is validated by making extensive comparisons of the pressures,displacements,and strains on various locations between the simulation and reported *** approach is appealing for dynamic analysis of other prestressed structures,owing to the good popularity and acknowledgement of ANSYS and Fluent in both academia and industry.

关键词： Loss-of-coolant accident(LOCA) Fluid–structure interaction(FSI) Finite element method Prestressed structure Structural dynamics

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文化环境建设与社区教育发展

江苏广播电视大学学报 2004年第2期15卷 5-10页

作者：陈乃林江苏广播电视大学江苏南京210036

社区教育的发展,受到文化传统、区域文化特色、生活方式演变、大众传播媒介等因素的影响;要切实加强社区教育文化环境建设,把民族精神作为文化建设的核心,加强思想道德教育和文化科学知识的普及,倡导公共精神,发挥制度文化的社会功能。

关键词：社区教育文化文化环境