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re-expression of DIrAS3 and P53 induces apoptosis and impaired autophagy in head and neck squamous cell carcinoma

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Military Medical research 2021年第2期8卷 148-161页

作者： Zhe Liu douglas r.Hurst Xing Qu Li-Guang Lu Chen-Zhou Wu Yu-Yu Li Yi Li State Key Laboratory of Oral Diseases&National Clinical research Center for Oral Diseases West China Hospital of StomatologySichuan UniversityChengdu 610041China Department of Head and Neck Oncology West China Hospital of StomatologySichuan UniversityChengdu 610041China Department of Pathology University of Alabama at BirminghamBirminghamAL 35216USA Department of Evidence Based Stomatology West China Hospital of StomatologySichuan UniversityChengdu 610041China.

Background:p53 and DIrAS3 are tumor suppressors that are frequently silenced in *** this study,we sought to determine whether the concurrent re-expression of p53 and DIrAS3 could effectively induce head and neck squamous cell carcinoma(HNSCC)cell ***:CAL-27 and SCC-25 cells were treated with Ad-DIrAS3 and rAd-p53 to induce re-expression of DIrAS3 and p53 *** effects of DIrAS3 and p53 re-expression on the growth and apoptosis of HNSCC cells were examined by TUNEL assay,flow cytometric analysis and *** effects of DIrAS3 and p53 re-expression on Akt phosphorylation,oncogene expression,and the interaction of 4 E-BP1 with eIF4 E were determined by real-time PCr,Western blotting and immunoprecipitation *** ability of DIrAS3 and p53 re-expression to induce autophagy was evaluated by transmission electron microscopy,LC3 fluorescence microscopy and Western *** effects of DIrAS3 and p53 re-expression on HNSCC growth were evaluated by using an orthotopic xenograft mouse ***:TUNEL assay and flow cytometric analysis showed that the concurrent re-expression of DIrAS3 and p53 significantly induced apoptosis(Pric analysis revealed that DIrAS3 and p53 reexpression significantly inhibited proliferation and induced cell cycle arrest(Prrent re-expression of DIrAS3 and p53 down-regulated signal transducer and activation of transcription 3(STAT3)and upregulated p21WAF1/CIP1 and Bax(PrAS3 and p53 re-expression also inhibited Akt phosphorylation,increased the interaction of eIF4 E with 4 E-BP1,and reduced the expression of c-Myc,cyclin D1,vascular endothelial growth factor(VEGF),fibroblast growth factor(FGF),epidermal growth factor receptor(EGFr)and Bcl-2(Preover,the concurrent re-expression of DIrAS3 and p53 increased the percentage of cells with GFP-LC3 puncta compared with that in cells treated with control adenovirus(50.00%±4.55%vs.4.67%±1.25%,P<

关键词： DIrAS3 p53 Apoptosis Autophagy Head and neck squamous cell carcinoma

reinterpretation of histology of proximal colon polyps called hyperplastic in 2001

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World Journal of Gastroenterology 2009年第30期15卷 3767-3770页

作者： Omer Khalid Sofyan radaideh Oscar W Cummings Michael J O'Brien John r Goldblum douglas K rex Department of Medicine Indiana University Medical CenterIndianapolisIN 46202United States Department of Pathology Indiana University Medical CenterIndianapolisIN 46202United States Department of Anatomic Pathology Boston University Medical CenterBostonMA 02118United States Department of Anatomic Pathology Cleveland ClinicClevelandOH 44195United States Division of Gastroenterology/Hepatology Indiana University Medical CenterIndianapolisIN 46202United States

AIM： To evaluate how proximal colon polyps interpreted as hyperplastic polyps in 2001 would be interpreted by expert pathologists in 2007.METHODS： ≥ 5 mm in interpreted pathologists 2007 by 3 GI Forty consecutive proximal colon polyps size, removed in 2001, and originally as hyperplastic polyps by general at Indiana University, were reviewed in pathologists.CONCLUSION： Many polyps interpreted as hyperplastic in 2001 were considered sessile serrated lesions by GI pathologists in 2007, but there is substantial inter-observer variation amongst GI pathologists.

关键词： Colonoscopy Colorectal polyps Hyperplasticpolyps Inter-observer variability Serrated adenomas Sessile serrated polyps

Spatiotemporal Evolution of West Africa's Urban Landscape Characteristics Applying Harmonized DMSP-OLS and NPP-VIIrS Nighttime Light(NTL)Data

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Chinese Geographical Science 2022年第6期32卷 933-945页

作者： SONO douglas WEI Ye CHEN Zuoqi JIN Ying School of Geographical Sciences Northeast Normal UniversityChangchun 130024China Natural resources Institute of Jilin Province Changchun 130024China Key Laboratory of Spatial Data Mining and Information Sharing of Ministry of Education National&Local Joint Engineering Research Center of Satellite Geospatial Information TechnologyFuzhou UniversityFuzhou 35002China The Academy of Digital China Fuzhou UniversityFuzhou 350002China School of Humanities and Law Northeastern UniversityShenyang 110169China

Investigating urban expansion patterns aids in the management of urbanization and in ameliorating the socioeconomic and environmental issues associated with economic transformation and sustainable *** Harmonized Defense Meteorological Satellite Program-Operational Line-scan System(DMSP-OLS)and the Suomi National Polar-Orbiting Partnership-Visible Infrared Imagery radiometer Suite(NPP-VIIrS)Nighttime Light(NTL)data,this paper investigated the characteristics of urban landscape in West *** the harmonized NTL data,spatial comparison and empirical threshold methods were employed to detect urban changes from 1993 to *** examined the rate of urban change and calculated the direction of the urban expansion of West Africa using the center-of-gravity method for urban *** addition,we used the landscape expansion index method to assess the processes and stages of urban growth in West *** accuracy of urban area extraction based on NTL data were r^(2)=0.8314 in 2000,r^(2)=0.8809 in 2006,r^(2)=0.9051 in 2012 for the DMSP-OLS and the simulated NPP-VIIrS was r^(2)=0.8426 in 2018,by using Google Earth images as *** results indicated that there was a high rate and acceleration of urban landscapes in West Africa,with rates of 0.0160,0.0173,0.0189,and 0.0686,and accelerations of 0.31,0.42,0.54,and 0.90 for the periods of 1998–2003,2003–2008,2008–2013,and 2013–2018,*** expansion direction of urban agglomeration in West Africa during 1993–2018 was mainly from the coast to ***,cities located in the Sahel region of Africa and in the middle zone expanded from north to ***,the results showed that the urban landscape of West Africa was mainly in a scattered and disordered’diffusion’process,whereas only a few cities located in coastal areas experiencing the process of’coalescence’according to urban growth phase *** study provides urban planners with relevant insights for the urban expansion character

关键词： urban expansion nighttime light remote sensing DMSP-OLS(Defense Meteorological Satellite Program-Operational Line-scan System) NPP-VIIrS(Suomi National Polar-Orbiting Partnership-Visible Infrared Imagery radiometer Suite) spatiotemporal evolution West Africa

Design of drug-like hepsin inhibitors against prostate cancer and kidney stones

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Acta Pharmaceutica Sinica B 2020年第7期10卷 1309-1320页

作者： Vincent Blay Mu-Chun Li Sunita P.Ho Mashall L.Stoller Hsing-Pang Hsieh douglas r.Houston Division of Biomaterials and Bioengineering School of DentistryUniversity of California San FranciscoSan FranciscoCA 94143USA Department of Urology School of MedicineUniversity of California San FranciscoSan FranciscoCA 94143USA Institute of Biotechnology and Pharmaceutical research National Health Research InstitutesZhunanTaiwan 350China University of Edinburgh Institute of Quantitative BiologyBiochemistry and BiotechnologyEdinburghScotlandEH93BFUK

Hepsin,a transmembrane serine protease abundant in renal endothelial cells,is a promising therapeutic target against several cancers,particularly prostate *** is involved in the release and polymerization of uromodulin in the urine,which plays a role in kidney stone *** this work,we design new potential hepsin inhibitors for high activity,improved specificity towards hepsin,and promising ADMET *** ligands were developed in silico through a novel hierarchical *** pipeline explicitly accounts for off-target binding to the related serine proteases matriptase and HGFA(human hepatocyte growth factor activator).We completed the pipeline incorporating ADMET properties of the candidate inhibitors into custom multi-objective optimization *** ligands designed show excellent prospects for targeting hepsin via the blood stream and the urine and thus enable key experimental *** computational pipeline proposed is remarkably costefficient and can be easily adapted for designing inhibitors against new drug targets.

关键词： Virtual screening Docking Library Hepsin Tamm-Horsfall protein Biomineralization

Dietary choline activates the Ampk/Srebp signaling pathway and decreases lipid levels in Pacific white shrimp(Litopenaeus vannamei)

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Animal Nutrition 2023年第4期15卷 58-70页

作者： Jingjing Lu Xinyue Tao Jiaxiang Luo Tingting Zhu Lefei Jiao Peng Sun Qicun Zhou douglas r.Tocher Min Jin Laboratory of Fish and Shellfish Nutrition School of Marine SciencesNingbo UniversityNingbo 315211China Guangdong Provincial Key Laboratory of Marine Biotechnology Institute of Marine SciencesShantou UniversityShantou 515063China

An 8-week feeding trial was conducted in Pacific white shrimp(Litopenaeus vannamei)to evaluate the effects of dietary choline supplementation on choline transport and metabolism,hepatopancreas histological structure and fatty acid profile,and regulation of lipid *** isonitrogenous and isolipidic diets were formulated to contain different choline levels of 2.91(basal diet),3.85,4.67,6.55,10.70 and 18.90 g/kg,respectively.A total of 960 shrimp(initial weight,1.38±0.01 g)were distributed randomly into twenty-four 250-L cylindrical fiber-glass tanks,with each diet assigned randomly to 4replicate *** results indicated that dietary choline significantly promoted the deposition of choline,betaine and carnitine(Prs and areas of r cells,total lipid and triglyceride contents in hepatopancreas,and triglyceride and non-esterified fatty acid contents in hemolymph were negatively correlated with dietary choline *** contents of functional fatty acids in hepatopancreas,the activity of acetyl-CoA carboxylase(Acc),and the mrNA expression of fas,srebp and acc were highest in shrimp fed the diet containing 4.67 g/kg choline,and significantly higher than those fed the diet containing 2.91 g/kg,the lowest level of choline(Pr of r cells,content of very lowdensity lipoprotein(VLDL),activities of carnitine palmitoyl-transferase(Cpt1),lipoprotein lipase and hepatic lipase,and the mrNA expression levels of cpt1,fabp,fatp,ldlr,and ampk in hepatopancreas increased significantly as dietary choline increased(Preas m rNA expression levels of ctl1,ctl2,oct1,badh,bhmt,ck,cept,and cct were generally up-regulated as dietary choline level increased(Pry choline promoted the deposition of choline and its metabolites by up-regulating genes related to choline transport and ***,appropriate dietary choline level promoted the development of hepatopancreas r cells and maintained the n

关键词： Pacific white shrimp Choline Lipid Fatty acid Histology Ampk signaling pathway

Transition to forefoot strike reduces load rates more effectively than altered cadence

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Journal of Sport and Health Science 2020年第3期9卷 248-257页

作者： Erin E.Futrell K.douglas Gross Darcy reisman David r.Mullineaux Irene S.Davis Department of Physical Therapy Springfield CollegeSpringfieldMA 01109USA Spaulding National running Center Harvard Medical SchoolCambridgeMA 02138USA Department of Physical Therapy MGH Institute of Health ProfessionsBostonMA 02129USA Department of Physical Therapy University of DelawareNewarkDE 19713USA School of Sport and Exercise Science University of LincolnBrayford PoolLincolnBrayford PoolLN67TSUK Department of Physical Medicine and rehabilitation Harvard Medical SchoolBostonMA 02115USA

Background: Excessive vertical impacts at landing are associated with common running injuries. Two primary gait-retraining interventions aimed at reducing impact forces are transition to forefoot strike and increasing cadence. The objective of this study was to compare the shortand long-term effects of 2 gait-retraining interventions aimed at reducing landing ***: A total of 39 healthy recreational runners using a rearfoot strike and a cadence of 170 steps/min were randomized into cadence(CAD) or forefoot strike(FFS) groups. All participants performed 4 weeks of strengthening followed by 8 sessions of gait-retraining using auditory feedback. Vertical average load rates(VALr) and vertical instantaneous load rates(VILr) were calculated from the vertical ground reaction force curve. Both cadence and foot strike angle were measured using 3D motion analysis and an instrumented treadmill at baseline and at 1 week,1 month, and 6 months post ***: ANOVA revealed that the FFS group had significant reductions in VALr(49.7%) and VILr(41.7%), and changes were maintained long term. Foot strike angle in the FFS group changed from 14.2° dorsiflexion at baseline to 3.4° plantarflexion, with changes maintained long term. The CAD group exhibited significant reduction only in VALr(16%) and only at 6 months. Both groups had significant and similar increases in cadence at all follow-ups(CAD, +7.2% to 173 steps/min;and FFS, +6.1% to 172 steps/min).Conclusion: Forefoot strike gait-retraining resulted in significantly greater reductions in VALr and similar increases in cadence compared to cadence gait-retraining in the short and long term. Cadence gait-retraining resulted in small reductions in VALr at only the 6-month follow-up.

关键词： Foot strike Gait-retraining Physical therapy running Step rate

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填充设计的最大部分平行类

中国科学：数学 2023年第2期53卷 217-232页

作者： douglas r.Stinson 卫瑞中 David r.Cheriton School of Computer Science University of WaterlooWaterlooON N2L 3G1Canada Department of Computer Science Lakehead UniversityThunder BayON L3V 0B9Canada

设整数β(ρ,v,k)是最大部分平行类大小为ρ的任意(v,k)-填充设计中所包含的最大区组数目.对于k=3,该问题由Stinson (2021)引入并研究.本文主要研究k=4的情形,获得β(ρ,v,4)的上下界.本文给出最大部分平行类大小为ρ的(v,4)-填充设计... 详细信息

设整数β(ρ,v,k)是最大部分平行类大小为ρ的任意(v,k)-填充设计中所包含的最大区组数目.对于k=3,该问题由Stinson (2021)引入并研究.本文主要研究k=4的情形,获得β(ρ,v,4)的上下界.本文给出最大部分平行类大小为ρ的(v,4)-填充设计的具体构造.对于小的ρ值,所构造的填充设计的区组数非常靠近上界β(ρ,v,4).本文的一些方法可以推广到k>4的情形.

关键词：最大部分平行类填充设计最优设计组合设计

Identification of a decedent in a 103-year-old homicide case using forensic anthropology and genetic genealogy

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Forensic Sciences research 2022年第3期7卷 412-426页

作者： Amy r.Michael Samantha H.Blatt Mariyam Isa Anthony redgrave douglas H.Ubelaker Department of Anthropology University of New HampshireDurhamNCUSA Department of Anthropology Idaho State UniversityPocatelloIDUSA Department of Anthropology Texas Tech UniversityLubbockTXUSA redgrave research Forensic Services OrangeCAUSA Department of Anthropology National Museum of Natural HistorySmithsonian InstitutionWashingtonDCUS

Anthropologists are often the custodians of long-term unidentified human remains though their positions as curators of university or museum skeletal *** factors decrease the solvability of these legacy cases including the passage of time,the loss of provenience for specific cases,and lack of documentation or case *** anthropologists can contribute important information toward identification,it is often necessary to explore novel and cross-disciplinary strategies to resolve difficult cold *** long cold cases,the postmortem interval,in particular,may be difficult to estimate leading to further challenges in achieving *** advances in radiocarbon bomb pulse dating,isotope analysis,and actualistic studies have contributed to positive identification of unidentified human remains in some legacy cases,but may not be available to all forensic practitioners and law enforcement from resource-poor *** resources,as well as collaborating with professionals outside of forensic anthropology,is a useful strategy to pursue when anthropological methods are *** case study presented here demonstrates a collaborative approach between forensic anthropologists,forensic genetic genealogists,and law enforcement in a century-old *** dismembered and mummified parts of a male body were recovered in a remote cave in 1979 and again in *** forensic anthropologists creating and updating the biological profile over the decades from recovery to present,no identification was made until the application of forensic genetic genealogy(FGG)to the case in *** interpretations of bone microstructure and trauma analysis are presented for the case,alongside the historical documentation and“proof of life”evidence used by the genealogy team.A review of the FGG methods underscores the challenges in this case(*** endogamy,multiple aliases used by the victim)and the steps taken toward ***,a co

关键词： Forensic sciences forensic anthropology forensic genetic genealogy human identification cold case investigative genetic genealogy postmortem interval skeletal trauma

Measuring the Effectiveness of the Chinese Innovation System:A Global Value Chain Approach

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International Journal of Innovation Studies 2017年第1期1卷 57-71页

作者： douglas Zhihua Zeng Tianjin Normal University 393 West Binshui Ave.Tianjin 300387China World Bank 1818 H St.WashingtonDC 20433USA University of International Business&Economics 10 Huixin DongjieChaoyang DistrictBeijing 100029China Xi’an Jiaotong‐Liverpool University No.111 RenʹailuSuzhouJiangsu 215123China

This paper study argues that some disruptive new trends are surfacing with the emergence of new technologies,which makes an efficient and effective r&D and innovation system even more relevant for any country to compete at a global *** this backdrop,the study tries to measure the effectiveness of the Chinese innovation system,using the“r&D spending as percentage of GDP”as the input variable and the“contribution of r&D and related business activities to the value‐added manufacturing exports”as the output *** on a 59‐country sample in the OECD‐WTO TiVA database,the regression results show a significant and positive correlation between the two *** findings also complement with other existing empirical and qualitative studies in this *** overall findings show that China has achieved significant efficiency and effectiveness in its innovation system compared with many other countries;in fact,since 2009,the efficiency has ***,it still lags behind the major global *** means China still has some room to improve in order to transform its increasing inputs in r&D and technology innovation into commensurate *** situation calls for further improvements in its innovation ecosystem.

关键词： Innovation China Global Value Chain r&D Trade