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In-Vitro Micropropagation and Acclimatization of an Endangered Native Orchid Using Organic Supplements

American Journal of Plant Sciences 2022年第3期13卷 380-393页

作者： douglas de stefano Bárbara Nogueira Souza Costa Jason Downing Esmaeil Fallahi Amir Ali Khoddamzadeh department of Earth and Environment Institute of Environment Florida International University Miami FL USA Fairchild Tropical Botanic Garden Coral Gables FL USA Parma Research and Extension Center Department of Plant Sciences University of Idaho Parma ID USA

In-vitro propagation is a technique that provides a vital solution for the conservation of endangered orchid species. The media used in tissue culture can be modified through the addition of inexpensive organic materials as an alternative to expensive synthetic additives. Some organic sources, such as coconut water and fruit juice, contain significant amounts of vitamins, amino acids, and organic compounds which can act as growth regulators, making these organic sources excellent additives for in-vitro cultivation. The aim of this study was to develop a protocol for in-vitro micropropagation and acclimatization of Epidendrum nocturnum using organic supplements in the growth media and various substrates at the acclimatization stage. Banana powder, coconut water, and potato dextrose were added to a basal seed sowing media and evaluated for seed germination percentage and plantlet growth. In addition, various substrates such as coconut coir, horticultural charcoal, sphagnum moss, and wood bark were evaluated for height, number of leaves, and number of shoots in the acclimatization portion of this study. The culture medium with coconut water showed a greater germination percentage (71.00% and 76.75%) compared with the control (37.50% and 45.50%) at 60 and 90 days after seed sowing, respectively. Media with organic supplements showed greater values of plant length and number of roots compared with the control. The combination of coconut coir and horticultural charcoal was shown to be more efficient than the combination of sphagnum moss, horticultural charcoal, and wood bark, as results showed greater values of plant height and number of leaves at 30, 90, and 120 days after transplantation in acclimatization of E. nocturnum.

关键词： Tissue Culture Native Orchid Plant Growth Hormones Climate Change Epidendrum nocturnum

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Theoretical prediction of high melting temperature for a Mo–Ru–Ta–W HCP multiprincipal element alloy

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npj Computational Materials 2021年第1期7卷 49-52页

作者： Qi-Jun Hong Jan Schroers douglas Hofmann stefano Curtarolo Mark Asta Axel van de Walle School of Engineering Brown UniversityProvidenceRI 02912USA department of Mechanical Engineering and Materials Science Yale UniversityNew HavenCT 06511USA NASA Jet Propulsion Laboratory California Institute of Technology4800 Oak Grove Dr.PasadenaCA 91109USA department of Mechanical Engineering and Materials Science and Center for Autonomous Materials design Duke UniversityDurhamNC 27708USA department of Materials Science and Engineering University of CaliforniaBerkeleyCA 94720USA Materials Sciences Division Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA

While rhenium is an ideal material for rapid thermal cycling applications under high temperatures,such as rocket engine nozzles,its high cost limits its widespread use and prompts an exploration of viable cost-effective *** prior work,we identified a promising pool of candidate substitute alloys consisting of Mo,Ru,Ta,and *** this work we demonstrate,based on density functional theory melting temperature calculations,that one of the candidates,Mo_(0.292)Ru_(0.555)Ta_(0.031)W_(0.122),exhibits a high melting temperature(around 2626 K),thus supporting its use in high-temperature applications.

关键词： alloy cycling high

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