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Phase separation of GRP7 facilitated by FERONIAmediated phosphorylation inhibits mRNA translation to modulate plant temperature resilience

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Molecular Plant 2024年第3期17卷 460-477页

作者： Fan Xu Long Wang Yingbin Li Junfeng Shi dorothee Staiger Feng Yu state Key Laboratory of Chemo/Biosensing and Chemometrics College of Biologyand Hunan Key Laboratory of Plant Functional Genomics and Developmental RegulationHunanUniversityChangsha 410082P.R.China State Key Laboratory of Hybrid Rice Hunan Agricultural Biotechnology Research InstituteHunan Academy of Agricultural SciencesChangsha 410125P.R.China RNA Biology and Molecular Physiology Faculty of BiologyBielefeld University33615 BielefeldGermany

Changes in ambient temperature profoundly affect plant growth and ***,the molecu-larbasis of plant acclimation to temperature fluctuation is of great *** this study,we discovered that GLYCINE-RICH RNA-BINDING PROTEIN 7(GRP7)contributes to cold and heat tolerance in Arabidopsis *** found that exposure to a warm temperature rapidly induces GRP7 condensates in planta,which can be reversed by transfer to a lower *** biology and biochemical assays revealed that GRP7 undergoes liquid-liquid phase separation(LLPS)in vivo and in *** of GRP7 in the cyto-plasm contributes to the formation of stress granules that recruit RNA,along with the translation machinery component eukaryotic initiation factor 4E1(elF4E1)and the mRNA chaperones COLD SHOCK PROTEIN 1(CSP1)and CSP3,to inhibit ***,natural variations in GRP7 affecting the residue phos-phorylated by the receptorkinase FERONIA alter its capacity to undergo LLPS and correlate with the adap-tation of some Arabidopsis accessions to a widertemperature *** together,ourfindings illustrate the role of translational control mediated by GRP7 LLPS to confer plants with temperature resilience.

关键词： fluctuating temperature glycine-rich RNA-binding protein phase separation cold shock proteins translation

Protective and Regenerative Efficacy of a Plant Oil-Based Day and Night Cream: Investigated by a Novel Approach to Reveal the Impact of Blue Light Irradiation on Epidermal Barrier Integrity and Lipid Matrix

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Journal of Cosmetics, Dermatological Sciences and Applications 2024年第3期14卷 227-241页

作者： dorothee Dähnhardt Stephan Dähnhardt-Pfeiffer Isabel Simon Dana Ditgen Inge Holland Dörte Segger Peter Staib Microscopy Services Dhnhardt GmbH Flintbek Germany Research & Development and Regulatory Affairs Kneipp GmbH Wuerzburg Germany SGS INSTITUT FRESENIUS GmbH Hamburg Germany

In recent years, the harmful effects of blue light (400 - 500 nm) as a component of visible light (400 - 700 nm) have increasingly gained attention of science, industry, and consumers. To date, only a few in vivo test methods for measuring the effects of blue light on the skin have been described. A direct measurement method that can detect the immediate effects of blue light on the epidermal permeability barrier (EPB) is still lacking. In this study, we present a new methodological approach that can be used to investigate both the protective and regenerative effects of cosmetic products on the EPB after blue light irradiation. In a study with 14 female volunteers, it was investigated whether the regular application of an O/W emulsion (day cream) can strengthen and protect the epidermal barrier against damaging blue light radiation of 60 J/cm2 (protective study design) and also whether a disruption of the epidermal barrier caused by blue light radiation is restored faster and better by the regular application of another O/W emulsion (night cream) than in product-untreated skin (regenerative study design). The two O/W emulsions are different in plant oil, active ingredient composition and texture. The seven-day treatment with the day cream initially led to a significant increase in the normalized lipid lamellae length in the intercellular space, whereas the irradiation with blue light after 24 hours led to a significant decrease in the lipid lamellae length in the untreated test area, but not in the area previously treated with the product. Regarding the regenerative study design, a two-day treatment with the night cream was able to restore a blue-light-induced decrease in lipid lamellae length in the intercellular space. In summary, with the study designs presented here, the protective and regenerative effect of two cosmetic products could be demonstrated for the first time on the integrity of the EPB after blue light irradiation and the data showed that the Lipbarv

关键词： Skin Care Blue Light Irradiation Skin Barrier Intercellular Lipid Lamellae Electron Microscopy

Marking RNA:m^6A writers,readers,and functions in Arabidopsis

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Journal of Molecular Cell Biology 2019年第10期11卷 899-910页

作者： Marlene Reichel Tino Koster dorothee Staiger RNA Biology and Molecular Physiology Faculty of BiologyBielefeld University33615 BielefeldGermany

N^6-methyladenosine(m^6A)emerges as an important modification in eukaryotic mRNAs.m^6A has first been reported in 1974,and its functional significance in mammalian gene regulation and importance for proper development have been well *** arsenal of writer,eraser,and reader proteins accomplish deposition,removal,and interpretation of the m^6A mark,resulting in dynamic *** led to the concept of an epitranscriptome,the compendium of RNA species with chemical modification ofthe nucleobases in the cell,in analogy to the *** m^6A has long been known to also exist in plant mRNAs,proteins involved in m^6A metabolism have only recently been detected by mutant analysis,homology search,and mRNA interactome capture in the reference plant Arabidopsis *** ofthe m^6A modification causes severe developmental abnormalities of leaves and roots and altered timing of reproductive ***,m^6A modification affects viral ***,we discuss recent progress in identifying m^6A sites transcriptome-wide,in identifying the molecular players involved in writing,removing,and reading the mark,and in assigning functions to this RNA modification in *** highlight similarities and differences to m^6A modification in mammals and provide an outlook on important questions that remain to be addressed.

关键词： Arabidopsis m^6A mRNA interactome posttranscriptional RNA-binding protein

Simulating small-scale climate change effects-lessons from a short-term field manipulation experiment on grassland arthropods

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Insect Science 2013年第5期20卷 662-670页

作者： Sascha Buchholz dorothee Rolfsmeyer Jens Schirmel Department of Ecology TU Berlin Institute of Landscape Ecology University of Munster Robert-Koch-Straβe 26 48149 Munster Ecosystem Analysis University ofKoblenz-Landau Fortstraβe 7 76829 Landau Germany

Climate change is expected to cause major consequences on biodiversity. Understanding species-specific reactions, such as species shifts, species declines, and changes in population dynamics is a key issue to quantify large-scale impacts of climate change on biotic communities. As it is often impossible or at least impracticable to conduct large-scale experiments on biotic responses to climate change, studies at a smaller scale may be a useful alternative. In our study, we therefore tested responses of grassland arthropods （carabid beetles, spiders, grasshoppers） to simulated climate change in terms of species activity densities and diversity. We conducted a controlled field experiment by changing water and microclimatic conditions at a small scale （16 m2）. Roof constructions were used to increase drought-like conditions, whereas water supply was enhanced by irrigation. In all, 2 038 carabid beetles （36 species）, 4 893 spiders （65 species）, and 303 Orthoptera （4 species） were caught using pitfall traps from May to August, 2010. During our experiment, we created an artificial small-scale climate change; and statistics revealed that these changes had short-term effects on the total number of individuals and Simpson diversity of the studied arthropod groups. Moreover, our results showed that certain species might react very quickly to climate change in terms of activity densities, which in turn might influence diversity due to shifts in abundance patterns. Finally, we devised methodological improvements that may further enhance the validity of future studies.

关键词： carabid beetles drought global wanning grasshoppers irrigation spiders

Continuous Embeddings of Besov-Morrey Function Spaces

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Acta Mathematica Sinica,English Series 2012年第7期28卷 1307-1328页

作者： dorothee D. HAROSKE Leszek SKRZYPCZAK Mathematical Institute Friedrich-Schiller-University Jena Faculty of Mathematics and Computer Science Adam Mickiewicz University

We study embeddings of spaces of Besov-Morrey type, MB p1^s1 q1^r1（R^d）→MB p2^s2 q2^r2（R^d） and obtain necessary and sufficient conditions for this. Moreover, we can also charaeterise the special weighted situation Bp1^s1 （R^d ,w）→MB p2^s2 q2^r2（R^d） for a Muekenhoupt A∞ weight w, with wα（x） = ｜x｜^a, 〉 -d, as a typical example.

关键词： Weighted Besov spaces Besov Morrey spaces continuous embeddings

Morrey smoothness spaces:A new approach

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Science China Mathematics 2023年第6期66卷 1301-1358页

作者： dorothee D.Haroske Hans Triebel Institute of Mathematics Faculty of Mathematics and Computer ScienceFriedrich Schiller University JenaJena 07743Germany

In the recent years,the so-called Morrey smoothness spaces attracted a lot of *** can(also)be understood as generalisations of the classical spaces A_(p,q)^(s)(R^(n))with A∈{B,F}in R^(n),where the parameters satisfy ... 详细信息

关键词： Morrey space smoothness space of Morrey type Besov-Morrey space Triebel-Lizorkin-Morrey space Besov-type space Triebel-Lizorkin-type space

Some Specific Unboundedness Property in Smoothness Morrey Spaces. The Non-existence of Growth Envelopes in the Subcritical Case

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Acta Mathematica Sinica,English Series 2016年第2期32卷 137-152页

作者： dorothee D.HAROSKE Susana D.MOURA Institute of Mathematics Priedrich-Schiller-University Jena CMUC Department of MathematicsUniversity of Coimbra

Abstract We study smoothness spaces of Morrey type on Rn and characterise in detail those situa s,r n s n tions when such spaces of type Ap,q^s,r（Rn ） or A u^sp,q（R ） are not embedded into L∞（R^n）. We can show that in the so-called sub-critical, proper Morrey case their growth envelope function is always infinite which is a much stronger assertion. The same applies for the Morrey spaces Mu,p（Rn） with p 〈 u. This is the first result in this direction and essentially contributes to a better understanding of the structure of the above spaces.

关键词： Besov-type space Morrey space Besov-Morrey space Triebel-Lizorkin-Morrey space,growth envelope atomic decomposition

Unboundedness properties of smoothness Morrey spaces of regular distributions on domains

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Science China Mathematics 2017年第12期60卷 2349-2376页

作者： HAROSKE dorothee D. MOURA Susana D. SCHNEIDER Cornelia SKRZYPCZAK Leszek Institute of Mathematics Friedrich Schiller University Jena CMUC Department of Mathematics University of Coimbra Mathematics Department Friedrich-Alexander University Erlangen-Nremberg Faculty of Mathematics and Computer Science Adam Mickiewicz University

We study unboundedness of smoothness Morrey spaces on bounded domains ? ? R^n in terms of growth envelopes. It turns out that in this situation the growth envelope function is finite—in contrast to the results obtained by Haroske et al.(2016) for corresponding spaces defined on R^n. A similar effect was already observed by Haroske et al.(2017), where classical Morrey spaces M_(u,p)(?) were investigated. We deal with all cases where the concept is reasonable and also include the tricky limiting cases. Our results can be reformulated in terms of optimal embeddings into the scale of Lorentz spaces L_(p,q)(?).

关键词： Morrey spaces Besov spaces Triebel-Lizorkin spaces growth envelopes atomic decompositions inequalities

A New Set of Reversibly Photoswitchable Fluorescent Proteins for Use in Transgenic Plants

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Molecular Plant 2013年第5期6卷 1518-1530页

作者： Martina Lummer Fabian Humpert Matthias Wiedenubbert Markus Sauer Mark Schuttpelz dorothee Staiger Molecular Cell Physiology Bielefeld University Universitatsstr. 25 33615 Bielefeld Germany Institute for Genome Research and Systems Biology CeBiTec 33615 Bielefeld Germany Biomolecular Photonics Bielefeld University Universitatsstr. 25 33615 Bielefeld Germany Biotechnology and Biophysics Julius-Maximilians-University Wurzburg Am Hubland 97074 Wurzburg Germany

Fluorescent reporter proteins that allow repeated switching between a fluorescent and a non-fluorescent state in response to specific wavelengths of light are novel tools for monitoring of protein trafficking and super-resolu- tion fluorescence microscopy in living organisms. Here, we describe variants of the reversibly photoswitchable fluores- cent proteins rsFastLime, bsDronpa, and Padron that have been codon-optimized for the use in transgenic Arabidopsis plants. The synthetic proteins, designated rsFastLIME-s, bsDRONPA-s, and PADRON C-s, showed photophysical properties and switching behavior comparable to those reported for the original proteins. By combining the ＇positively switchable＇ PADRON C-s with the ＇negatively switchable＇ rsFastLIME-s or bsDRONPA-s, two different fluorescent reporter proteins could be imaged at the same wavelength upon transient expression in Nicotiana benthamiana cells. Thus, co-localiza- tion analysis can be performed using only a single detection channel. Furthermore, the proteins were used to tag the RNA-binding protein AtGRP7 （Arabidopsis thaliana glycine-rich RNA-binding protein 7） in transgenic Arabidopsis plants. Because the new reversibly photoswitchable fluorescent proteins show an increase in signal strength during each pho- toactivation cycle, we were able to generate a large number of scans of the same region and reconstruct 3-D images of AtGRP7 expression in the root tip. Upon photoactivation of the AtGRP7：rsFastLIME-s fusion protein in a defined region of a transgenic Arabidopsis root, spreading of the fluorescence signal into adjacent regions was observed, indicating that movement from cell to cell can be monitored. Our results demonstrate that rsFastLIME-s, bsDRONPA-s, and PADRON C-s are versatile fluorescent markers in plants, Furthermore, the proteins also show strong fluorescence in mammalian cells including COS-7 and HeLa cells.

关键词： reversibly photoswitchable fluorescent protein codon usage Arabidopsis rsFastLIME-s bsDRONPA-s PADRON-s.