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Investigation of the immune effects of Scutellaria baicalensis on blood leukocytes and selected organs of the chicken＇s lymphatic system

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Journal of Animal Science and Biotechnology 2017年第3期8卷 601-612页

作者： Bozena Kroliczewska Stanislaw Graczyk Jaroslaw Kroliczewski Aleksandra Pliszczak-Krol dorota mista Wojciech Zawadzki Department of Animal Physiology and Biostructure Faculty of Veterinary Medicine Wroclaw University of Environmental and Life Sciences C.K Norwida 31 50-375 Wrodaw Poland Department of Immunology Pathophysiology and Veterinary Preventive Medicine Faculty of Veterinary Medicine Wrodaw University of Environmental and Life Sciences Wrodaw Poland Department of Chemical Biology Faculty of Biotechnology University of Wroclaw Fryderyka Joliot-Curie 14a 50-383 Wroclaw Poland

Background： The health of chickens and the welfare of poultry industry are central to the efforts of addressing global food security. Therefore, it is essential to study chicken immunology to maintain and improve its health and to find novel and sustainable solutions. This paper presents a study on investigation of the effect of Scutellaria baicalensis root（SBR） on the immune response of broiler chicken, especially on lymphocytes and heterophils reactivity, regarding their contribution to the development of immunity of the ***： The 121-day-old Hubbard Hi-Y male broiler hybrids were randomly assigned to four treatment groups,three SBR supplemented groups（0.5, 1.0, and 1.5% of SBR） and one control group. Each treatment was replicated five times with six birds per replicate pen in a battery brooder. Blood was collected after 3-（rd） and 6-（th）wk of the experiment, and hemoglobin and hematocrit values were determined, as well as total leukocyte count and differential count were performed. Nitroblue tetrazolium test and phagocytosis assay as nonspecific immune parameters and humoral immune responses to the antigenic challenge by sheep red blood cells were ***, the ability of peripheral blood lymphocytes to form radial segmentation（RS） of their nuclei was *** weight and relative weight of spleen, liver, and bursa of Fabricius were ***： Results showed that mean heterophile/lymphocyte ratio increased in the SBR groups compared to the control group and the blood of the chickens showed lymphocytic depletion. The results also demonstrated that the relative weight of bursa of Fabricius and spleen in groups fed with SBR significantly decreased compared to the control group. This study also showed that the addition of SBR significantly inhibited the formation of RS of nuclei compared to some cytotoxic ***： We found that SBR supplementation should be carefully evaluated when given to poultry. The excess intake of

关键词： Development of immune organs Leukocyte Lymphatic system Radial segmentation Scutellaria baicalensis Toxic effect

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Antiviral factors and their counteraction by HIV-1: many uncovered and more to be discovered

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Journal of Molecular Cell Biology 2024年第2期16卷 1-10页

作者： dorota Kmiec Frank Kirchhoff Institute of Molecular Virology Ulm University Medical Center89081 UlmGermany

Extensive studies on HIV-1 have led to the discovery of a variety of structurally and functionally diverse innate defense factors that target various steps of the retroviral replication *** of them,such as APOBEC3,tetherin,and SERINC5,are well *** importance is evident from the fact that HIV-1 uses its accessory proteins Vif,Vpu,and Nef to counteract ***,the list of antiviral factors is constantly increasing,and accumulating evidence suggests that innate defense mechanisms,which restrict HIV-1 and/or are counteracted by viral proteins,remain to be *** antiviral factors are relevant to diseases other than HIV/AIDS,since they are commonly active against various viral *** this review,we provide an overview of recently reported antiretroviral factors and viral countermeasures,present the evidence suggesting that more innate defense mechanisms remain to be discovered,and discuss why this is a challenging but rewarding task.

关键词： HIV-1 restriction factors viral antagonists interferon-stimulated genes antiviral factors

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Changes in the adult sex ratio of six duck species breeding populations over two decades

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Avian Research 2024年第2期15卷 202-210页

作者： Monika Homolkova Petr Musil Diego Pavon-Jordan dorota Gajdosova Zuzana Musilova Sarka Neuzilova Jan Zouhar Department of Ecology Faculty of Environmental SciencesCzech University of Life Sciences PragueKamýcka 129CZ-16521Prague–SuchdolCzech Republic Department of Terrestrial Ecology Norwegian Institute for Nature Research(NINA)PO Box 5685 TorgardenTrondheimN-7485Norway Department of Econometrics Faculty of Informatics and StatisticsUniversity of Economicsnam.W.Churchilla 4CZ-13067Prague 3Czech Republic

Despite all efforts,long-term changes in the adult sex ratios of breeding duck populations are still unclear;this uncertainty is especially true for male-bias populations,which are often under the scrutiny of researchers lacking convenient results for the active protection of endangered *** with male-bias populations are usually strongly affected by a decline in population size that leads to a higher extinction *** this study,we examined our long-term data of the abundance of breeding populations in six duck species(Mallard Anas platyrhynchos,Gadwall Mareca strepera,Red-crested Pochard Netta rufina,Common Pochard Aythya ferina,Tufted Duck Aythya fuligula,and Common Goldeneye Bucephala clangula)from fishponds in South Bohemia,Czechia,between 2004 and *** evidence was used to assess long-term changes in the adult sex ratio in these breeding populations and investigate the possible effects of the NAO index(North Atlantic Oscillation index)on them,indicating climate conditions in *** determined a long-term decrease of the proportion of females in the breeding season in two of the six examined species:Common Pochard and Red-crested Pochard,which is driven by the long-term increase in the number of males in contrast to the decreasing or stable number of females likely caused by different migration behaviours between females and *** the case of Common Pochard,in breeding populations,we estimated 60-65%of males in the early 2000s rising to 75-80%in the early ***,we establish no significant effects linked to climate conditions of the previous winter in these species as a crucial cause of the changes of the proportion of females in the breeding population.

关键词： Adult sex ratio Breeding population Population dynamics Red-listed species Waterfowl Winter weather conditions

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A single degenerated primer significantly improves COX1 barcoding performance in soil nematode community profiling

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Soil Ecology Letters 2024年第2期6卷 31-44页

作者： Yincai Ren dorota L. Porazinska Quanhui Ma Shuhan Liu Hongmei Li Xue Qing Department of Plant Pathology Nanjing Agricultural UniversityNanjing210095China Department of Entomology and Nematology University of FloridaGainesvilleFL32611USA Institute of Grassland Science Key Laboratory of Vegetation Ecology of the Ministry of EducationJilin Songnen Grassland Ecosystem National Observation and Research StationNortheast Normal UniversityChangchun130024China

A new COX1 primer for soil nematode metabarcoding was designed,and this primer outperforms other commonly used COX1 primer pairs in species recovery and quantity of PCR products.•The lack of reference database is the main reason that led to the low species recovery in COX1 metabarcoding.•We expanded current NCBI database by adding 51 newly generated COX1 reference *** nematodes play important roles in soil ecosystems and often serve as bioindicators of soil *** identification of soil nematodes is often difficult due to their limited diagnostic characters and high phenotypic *** barcoding and metabarcoding techniques are promising but lack universal primers,especially for mitochondrial COX1 *** this study a degenerated COX1 forward primer COIFGED was *** primer pair(COIFGED/JB5GED)outperforms other four commonly used COX1 primer pairs in species recovery and quantity of polymerase chain reaction(PCR)*** metabarcoding analysis,the reads obtained from the new primer pair had the highest sequencing saturation threshold and amplicon sequence variant(ASV)diversity in comparison to other COX1 as well as 18S rRNA *** annotation of ASVs suggested the new primer pair initially recovered 9 and 6 out of 25 genera from mock communities,respectively,outperformed other COX1 primers,but underperformed the widely used 18S NF1/18Sr2b primers(16 out of 25 genera).By supplementing the COX1 database with our reference sequences,we recovered an additional 6 mock community species bringing the tally closer to that obtained with 18S *** summary,our newly designed COX1 primers significantly improved species recovery and thus can be supplementary or alternative to the conventional 18S metabarcoding.

关键词： degenerated primers DNA metabarcoding mitochondrial cytochrome oxidase c subunit I gene phylogeny ribosomal RNA gene soil nematodes

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Fuel poverty and low carbon emissions: a comparative study of the feasibility of the hybrid renewable energy systems incorporating combined heat and power technology

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Frontiers in Energy 2022年第2期16卷 336-356页

作者： dorota RZETELSKA Madeleine COMBRINCK Department of Mechanical and Construction Engineering Faculty of Engineering and EnvironmentNorthumbria UniversityNewcastle-upon-TyneNE18STUK

Fuel poverty is most prevalent in North East England with 14.4%of fuel poor households in Newcastle upon *** aim of this paper was to identify a grid connected renewable energy system coupled with natural gas reciprocating combined heat and power unit,that is cost-effective and technically feasible with a potential to generate a profit from selling energy excess to the grid to help alleviate fuel *** system was also aimed at low carbon *** models were designed and optimized with the aid of the HOMER Pro *** were compared with respect to their economic,technical,and environmental performance.A solution was proposed where restrictions were placed on the size of renewable energy *** configuration consists of 150 kW CHP,300 kW PV cells,and 30 kW wind *** renewable fraction is 5.10%and the system yields a carbon saving of 7.9%in comparison with conventional *** initial capital investment is$1.24 million which enables the system to have grid sales of 582689 kWh/a.A conservative calculation determined that 40%of the sales can be used to reduce the energy cost of fuel poor households by$706 per *** solution has the potential to eliminate fuel poverty at the site analyzed.

关键词： greenhouse gas control low carbon target grid connected renewable fraction fuel poverty combined heat and power HOMER Pro

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Effects of hyperbaric oxygen therapy on human psychomotor performance:A review

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结合医学学报:英文版 2023年第5期21卷 430-440页

作者： dorota Olex-Zarychta Institute of Sport Sciences Academy of Physical Education in Katowice40-065 KatowicePoland

Psychomotor performance is the coordination of a sensory or ideational(cognitive)process and a motor *** sensorimotor processes involved in planning and execution of voluntary movements need oxygen supply and seem to be significantly disrupted in states of *** oxygen therapy has become a widely used treatment in routine medicine and sport medicine due to its beneficial effects on different aspects of human physiology and *** paper presents state-of-the-art data on the effects of hyperbaric oxygen therapy on different aspects of human psychomotor *** therapy’s influence on musculoskeletal properties and motor abilities as well as the effects of hyperbaric oxygenation on cognitive,myocardial and pulmonary functions are *** this review the molecular and physiological processes related to human psychomotor performance in response to hyperbaric oxygen are discussed to contribute to this fast-growing field of research in integrative medicine.

关键词： Hyperbaric oxygen therapy Hypoxia Psychomotor performance Oxidative metabolism

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Application of monodispersive anion exchangers in sorption and separation of Y^3＋ from Nd^3＋ and Sm^3＋ complexes with dcta

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Journal of Rare Earths 2008年第5期26卷 619-625页

作者： Halina Hubicka dorota Kolodynska Department of Inorganic Chemistry Faculty of Chemistry Maria Curie-Sklodowska University Maria Curie-Sklodowska Sq.3 20-031 Lublin Poland

Rare earth complexes with trans-1,2-diaminocyclohexane-N,N,N ,N -tetraacetic acid （DCTA） of the Ln（dcta） type exhibited an unusual sequence of affinity on the polystyrene anion exchangers： pm^3＋〉Nd^3＋〉Sm^3＋〉Pr^3＋〉Ce^3＋〉Eu^3＋〉Gd^3＋〉La^3＋〉Sc^3＋〉Tb^3＋〉Dy^3＋〉 Ho^3＋〉Y^3＋〉Er^3＋〉Tm^3＋〉Yb3＋〉Lu^3＋[1]. Taking into account the position of Y^3＋, Sm^3＋, and Nd^3＋ in this affinity series, for the monodispersive polystyrene anion exchangers, Lewatit MonoPlus M 500, Lewatit MonoPlus M 600, Lewatit MonoPlus MP 500, Lewatit MonoPlus MP 64, and for the heterodispersive anion exchanger, Lewatit MP 62, the weight （Dg） and bed （Dv） distribution coefficients of these complexes and working ion exchange capacities （Cw） were determined. Based on these values, purifications of Y^3＋ from Nd^3＋ and Y^3＋ from Sm^3＋ in the macro-micro component system on these anion exchangers were studied. The application potential of this method was highlighted for the separation of Y^3＋ in the presence of Nd^3＋ and Sm^3＋. With 1 L of monodispersive and strongly basic polystyrene gel anion exchanger Lewatit MonoPlus M 500 in the acetate form, it is possible to obtain approximately 79 g Y2O3 purified from Nd2O3 and 70 g Y2O3 purified from Sm2O3 in the same process condition.

关键词： DCTA rare earth complexes anion exchange

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Effects of Polar Organic Solvent on Separation of Y(edta)^-/Nd(edta)^- Complexes on Polyacrylic Anion Exchangers

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Journal of Rare Earths 2005年第1期23卷 124-128页

作者： Halina Hubicka dorota Kolodynska Department of Inorganic Chemistry Faculty of Chemistry Maria Curie-Sklodowska University Maria Curie-Sklodowska Sq.3 20-031 Lublin PolandDepartment of Inorganic Chemistry Faculty of Chemistry Maria Curie-Sklodowska University Maria Curie-Sklodowska Sq.3 20-031 Lublin Poland

The use of polar organic solvents for the separations of rare earth elements (Ⅲ) is effective especially for their extensive separations despite the solubility limitations. The study shows that polyacrylate anion exchangers, particularly the weakly basic, gel anion exchanger Amberlite IRA 68, can be applied to the separation of rare earth complexes with EDTA in H_2O-methanol and H_2O-ethanol systems. In most cases the determined distribution coefficients of Ln^(3+) complexes with EDTA in mixed media like water-methanol on polyacrylate anion exchangers are larger than those in pure water (media.)

关键词： polar organic solvent Y(edta)^-/Nd(edta)^- separation ion exchange rare earths

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Diversity of Saccharomyces boulardii CNCM I-745 mechanisms of action against intestinal infections

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World Journal of Gastroenterology 2019年第18期25卷 2188-2203页

作者： dorota Czerucka Patrick Rampall Department of Human Health Division of Ecosystems and Immunity Center Scientific of Monaco Center Scientific of Monaco

The yeast Saccharomyces boulardii CNCM I-745 is one of the probiotics recommended for the prevention of antibiotic-associated diarrhea. Studies conducted in vivo and in vitro demonstrated that in the case of infectious diseases there are two potential sites of action of Saccharomyces boulardii CNCM I-745:(1)An action on enteropathogenic microorganisms(adhesion of bacteria and their elimination or an effect on their virulence factors: Toxins, lipopolysaccharide,etc.); and(2) a direct action on the intestinal mucosa(trophic effects, effects on epithelial reconstitution, anti-secretory effects, anti-inflammatory,immunomodulators). Oral administration of Saccharomyces boulardii CNCM I-745 to healthy subjects does not alter their microbiota. However, in the case of diseases associated with the use of antibiotics or chronic diarrhea, Saccharomyces boulardii CNCM I-745 can restore the intestinal microbiota faster. The interaction of Saccharomyces boulardii CNCM I-745 with the innate immune system have been recently demonstrated thus opening up a new therapeutic potential of this yeast in the case of diseases associated with intestinal infections but also other pathologies associated with dysbiosis such as inflammatory diseases.

关键词： Saccharomyces boulardii CNCM I-745 Probiotics Yeast Intestinal infection Mechanism

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Genotype 3-hepatitis C virus’last line of defense

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World Journal of Gastroenterology 2021年第11期27卷 1006-1021页

作者： dorota Zarębska-Michaluk Department of Infectious Diseases Jan Kochanowski UniversityKielce 25-369ŚwiętokrzyskiePoland

Chronic infection with hepatitis C virus(HCV)is one of the leading causes of liver disease globally,affecting approximately 71 million *** majority of them are infected with genotype(GT)1 but infections with GT3 are second in *** many years,GT3 was considered to be less pathogenic compared to other GTs in the HCV family due to its favorable response to interferon(IFN)-based ***,the growing evidence of a higher rate of steatosis,more rapid progression of liver fibrosis,and lower efficacy of antiviral treatment compared to infection with other HCV GTs has changed this *** review presents the specifics of the course of GT3 infection and the development of therapeutic options for GT3-infected patients in the era of direct-acting antivirals(DAA).The way from a standard of care therapy with pegylated IFNalpha(pegIFNα)and ribavirin(RBV)through a triple combination of pegIFNα+RBV and DAA to the highly potent IFN-free pangenotypic DAA regimens is discussed along with some treatment options which appeared to be dead *** the implementation of highly effective pangenotypic regimens is the most recent stage of revolution in the treatment of GT3 infection,there is still room for improvement,especially in patients with liver cirrhosis and those who fail to respond to DAA therapies,particularly those containing inhibitors of HCV nonstructural protein 5A.

关键词： Hepatitis C virus Genotype 3 Antiviral treatment Interferon Direct-acting antivirals Pangenotypic

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