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LKB1 depletion-mediated epithelial-mesenchymal transition induces fibroblast activation in lung fibrosis

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Genes & Diseases 2024年第3期11卷 417-426页

作者： Zijian Xu elizabeth R.davies Liudi Yao Yilu Zhou Juanjuan Li Aiman Alzetani Ben G.Marshall David Hancock Tim Wallis Julian Downward Rob M.ewing donna e.davies Mark G.Jones Yihua Wang Biological Sciences Faculty of Environmental and Life SciencesUniversity of SouthamptonSouthampton SO171BJUK clinical and experimental Sciences Faculty of MedicineUniversity of SouthamptonSouthampton SO166YDUK Institute for Life Sciences University of SouthamptonSouthampton SO171BJUK NIHR Southampton Biomedical Research Centre University Hospital SouthamptonSouthampton SO166YDUK University Hospital Southampton Southampton SO166YDUK Oncogene Biology The Francis Crick InstituteLondon NW11ATUK

The factors that determine fibrosis progression or normal tissue repair are largely *** previously demonstrated that autophagy inhibition-mediated epithelial-mesenchymal transition(eMT)in human alveolar epithelial type Il(ATIl)cells augments local myofibroblast differentiation in pulmonary fibrosis by paracrine ***,we report that liver kinase B1(LKB1)inactivation in ATIl cells inhibits autophagy and induces eMT as a *** IPF lungs,this is caused by the down-regulation of CAB39L,a key subunit within the LKB1 complex.3D co-cultures of ATIl cells and MRC5 lung fibroblasts coupled with RNA sequencing(RNA-seq)confirmed that paracrine signaling between LKB1-depleted ATIl cells and fibroblasts augmented myofibroblast ***,these data suggest that reduced autophagy caused by LKB1 inhibition can induce eMT in ATIl cells and contribute to fibrosis via aberrant epithelial-fibroblast crosstalk.

关键词： CAB39L Crosstalk eMT LKB1 Pulmonary fibrosis

Identification of host miRNAs that may limit human rhinovirus replication

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World Journal of Biological Chemistry 2014年第4期5卷 437-456页

作者： Victor Paky Bondanese Ana Francisco-Garcia Nicole Bedke donna e davies Tilman Sanchez-elsner LGL Lab Ex LIO ENS Lyon Site Monod 69364 Lyon France Academic Unit of Clinical and experimental Sciences University of Southampton Faculty of Medicine University Hospital Southampton Southampton SO16 6YD United Kingdom Immunocore Limited Milton Park Abingdon Oxon OX14 4RY United Kingdom

AIM: To test whether the replication of human rhinovirus(HRV) is regulated by micro RNAs in human bronchial epithelial ***: For the present study, the human cell line BeAS-2B(derived from normal human bronchial epithelial cells) was adopted. DICeR knock-down, by si RNA transfection in BeAS-2B cells, was performed in order to inhibit micro RNA maturation globally. Alternatively, antisense oligonucleotides(anti-mi Rs) were transfectedto inhibit the activity of specific micro RNAs. Cells were infected with HRV-1B. Viral replication was assessed by measuring the genomic viral RNA by reverse transcription quantitative polymerase chain reaction(RT-q PCR). Association between micro RNA-induced-silencing-complex and viral RNA was detected by Ago2 co-immunoprecipitation followed by RT-q PCR. Targetscan v.6 was used to predict micro RNA target sites on several HRV ***: Here, we show that micro RNAs affect replication of HRV-1B. DICeR knock-down significantly reduced the expression of mature micro RNAs in a bronchial epithelial cell line(BeAS-2B) and in turn, increased the synthesis of HRV-1B RNA. Additionally, HRV-1B RNA co-immunoprecipitated with argonaute 2 protein, an important effector for micro RNA activity suggesting that micro RNAs bind to viral RNA during infection. In order to identify specific micro RNAs involved in this interaction, we employed bioinformatics analysis, and selected a group of micro RNAs that have been reported to be under-expressed in asthmatic bronchial epithelial cells and were predicted to target different strains of rhinoviruses(HRV-1B,-16,-14,-27). Our results suggest that, out of this group of micro RNAs, mi R-128 and mi R-155 contribute to the innate defense against HRV-1B: transfection of specific anti-mi Rs increased viral replication, as anticipated ***: Taken together, our results suggest that pathological changes in micro RNA expression, as already reported for asthma or chronic obstructive pulmona

关键词： Human Rhinovirus Micro RNAs Mi R-155 Si RNA Lentiviral transduction Antiviral innate immunity

In situ chemical oxidation of aniline by persulfate with iron(Ⅱ) activation at ambient temperature

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Chinese Chemical Letters 2010年第8期21卷 911-913页

作者： Yong Qing Zhang Wei Lin Huang donna e.Fennell College of environmental Science and engineering South China University of TechnologyGuangzhou 510006China The Key Lab of Pollution Control and ecosystem Restoration in Industry Clusters Ministry of EducationGuangzhou 510006China Department of environmental Sciences RutgersThe State University of New JerseyNew BrunswickNJ 08901USA

The kinetics of aniline degradation by persulfate processes with iron（Ⅱ） activation at ambient temperature was investigated in this *** iron（Ⅱ） as initiator,the oxidation reactions were found to follow a biphasic rate phenomenon：a rapid transformation followed by a slow but sustained oxidation *** the first 30 s,the reaction mainly relies on the persulfate-Fe^（2＋） reaction in which aniline is oxidized *** 30 s,the aniline was still oxidized but the rate of reaction tended to be slower and the rates were clearly *** the initial fast oxidation,the reactions appeared to follow a pseudo-first-order model.

关键词： Persulfate Aniline In situ chemical oxidation

Neuroimmune actions in the brain and interactions with the effects of alcohol

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Neural Regeneration Research 2021年第9期16卷 1791-1792页

作者： donna L.Gruol Neuroscience Department The Scripps Research InstituteLa JollaCAUSA

The neuroimmune system of the brain:early studies(1990’s)on the neurological consequences of human immunodeficiency virus-1(HIV-1)infection in the brain were instrumental in establishing that specific brain cell types can function as an innate immune system within the brain and in that role influence cognitive function(Kaul et al.,2005).

关键词： Neuroimmune actions in the brain and interactions with the effects of alcohol

Wong's nursing care of infants and children / senior consultant, donna L. Wong ; primary author, Marilyn J. Hockenberry ; section editors, David Wilson, Marilyn L. Winkelstein, Nancy e. Kline. 7th ed.

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Wong's nursing care of infants and children / senior consult...

Histone Acetylation, VeRNALIZATION INSeNSITIVe 3, FLOWeRING LOCUS C, and the Vernalization Response

2003年

作者： senior consultant donna L. Wong primary author Marilyn J. Hockenberry section editors David Wilson Marilyn L. Winkelstein Nancy e. Kline.

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Molecular Plant 2009年第4期2卷 724-737页

作者： donna M. Bond elizabeth S. Dennis Barry J. Pogson e. Jean Finnegan CSIRO Plant Industry Canberra Australia ARC Centre of excellence in Plant energy Biology School of Biochemistry and Molecular Biology Australian National University Canberra Australia

The quantitative induction of VIN3 by low temperatures is required for PRC2 repression of FLC and promotion of flowering （vernalization） in Arabidopsis. Histone acetylation, a chromatin modification commonly associated with gene transcription, increased on VIN3 chromatin in two spatially and temporally distinct phases in response to low temperatures. During short-term cold exposure, histone H3 acetylation at the transcription start site rapidly increased, implying that it is required for VlN3 induction. Subsequent changes in histone H3 and H4 acetylation occurred following continued VIN3 transcription during prolonged cold exposure. Members of the SAGA-like transcriptional adaptor complex, including the histone acetyltransferase GCNS, which induces expression of the cold acclimation pathway genes, do not regulate VlN3 induction during cold exposure, indicating that the cold acclimation pathway and the cold-induction of VlN3 are regulated by different transcriptional mechanisms. Mutations in the other 11 histone acetyltransferase genes did not affect VlN3 induction. However, nicotinamide, a histone deacetyiase inhibitor, induced VIN3 and altered histone acetylation at the VIN3 locus. VIN3 induction was proportional to the length of nicotinamide treatment, which was associated with an early-flowering phenotype and repression of FLC. However, unlike vernalization, the repression of FLC was independent of VIN3 activity. Nicotinamide treatment did not cause a change in the expression of any genes in the autonomous pathway or members of the PRC2 complex, the well characterized repressors of FLC. Our data suggest that FLC is repressed via a novel pathway involving the SIR2 class of histone deacetylases.

关键词： Cold acclimation histone acetyltransferase histone deacetylase SIR2 nicotinamide.

Severe and hazardous weather ::an introduction to high impact meteorology / 2nd ed.

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趋磁细菌磁小体及其相关MRI报告基因分子影像学研究

2005年

作者： Robert M. Rauber John e. Walsh and donna J. Charl

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中国医学影像技术 2019年第4期35卷 625-629页

作者：张薇薇 donna e.Goldhawk 李海龙甘肃中医药大学医学影像教研室甘肃兰州730000 罗森健康研究所医学影像项目组西安大略大学医学生物物理学系甘肃中医药大学临床诊断检验教研室甘肃兰州730000 甘肃中医药大学甘肃省中医方药挖掘与创新转化重点实验室甘肃兰州730000

趋磁细菌受基因调控形成纳米Fe_3O_4颗粒——磁小体,自带天然脂性包膜,其独特结构与特性受到分子影像学研究的重视,特别是纯化磁小体在磁性粒子成像中的应用和磁小体相关调控基因在MRI报告基因的分子影像学中的应用。相关研究逐年增多,... 详细信息

趋磁细菌受基因调控形成纳米Fe_3O_4颗粒——磁小体,自带天然脂性包膜,其独特结构与特性受到分子影像学研究的重视,特别是纯化磁小体在磁性粒子成像中的应用和磁小体相关调控基因在MRI报告基因的分子影像学中的应用。相关研究逐年增多,但有关MRI报告基因的研究尚处于初级阶段。本文对趋磁细菌磁小体及其相关基因在MRI报告基因的分子影像学研究进行综述。

关键词：磁小体磁共振报告基因分子影像学

Advance Directives in the Context of Uncertain Prognosis for Residents of Nursing Homes

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Open Journal of Nursing 2014年第4期4卷 321-329页

作者： Beryl e. Cable-Williams donna M. donna M. Wilson Norah Keating Department of Human ecology University of Alberta Edmonton Canada Faculty of Nursing University of Alberta Edmonton Canada Trent/Fleming School of Nursing Trent University Peterborough Canada

This report documents the findings of a mixed-methods study focused on the advanced directives of 182 residents of three LTC facilities in southern Ontario, Canada. Although almost all had a completed advance directive within 3 months of death, most did not have a palliative designation or directive until a few days before they died. each facility’s written Progress Notes revealed staff members usually sought additional confirmation of care preferences from residents’ substitute decision-makers within a few days of the death. It was thus common for advance directives to change from a more interventionist approach to the least interventionist approach near death. This change indicates that the meaning and significance of advance care planning and resulting advance directives must be considered in light of the processes and temporal factors involved in their completion and use within this distinct population. The relational nature of advance care planning and concern about ageism as a factor for withholding or withdrawing life support for LTC residents are considered as possible explanatory factors. These findings and their implications are described in relation to end-of-life care policies and practices in LTC facilities.

关键词： Advance Care Planning Advance Directives Palliative Care Nursing Homes