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地黄饮子通过PI3K/Akt和MAPK信号通路双重调节db/db小鼠视网膜胰岛素抵抗和糖代谢异常的机制研究

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中华中医药学刊 2024年第8期42卷 112-116,I0020,I0021,I0022页

作者：武东燕王小丹桂婉威柴金苗李钦青贺文彬张俊龙郭蕾山西中医药大学山西晋中030600

目的探讨地黄饮子通过磷脂酰肌醇三激酶-蛋白激酶B(phosphoinositide 3-kinase/protein kinase B,PI3K/Akt)和丝裂原激活蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路双重调节db/db小鼠视网膜胰岛素抵抗和糖代谢异常,探究地黄饮子多途径发挥治疗糖尿病视网膜病变(diabetic retinopathy,DR)作用的分子机制。方法10只db/m小鼠作为空白组,30只db/db小鼠随机分为模型组、地黄饮子组(地黄饮子,30.03 g/kg)、阳性对照组(二甲双胍,0.61 g/kg)。药物干预4周,每周检测小鼠体质量、空腹血糖。末次给药后进行口服糖耐量试验(oral glucose tolerance test,OGTT)及胰岛素耐量试验(insulin tolerance test,ITT)并计算曲线下面积(area under the curve,AUC);葡萄糖氧化酶法检测小鼠视网膜葡萄糖含量;酶联免疫吸附法(EliSA)检测小鼠视网膜胰岛素含量;苏木素-伊红(HE)染色观察小鼠视网膜组织病理改变;免疫组化法检测视网膜组织胰岛素受体底物1(insulin receptor substrate 1,IRS1)、葡萄糖转运蛋白4(glucose transporter 4,GLUT4)表达;实时荧光定量聚合酶链式反应法(RT-qPCR)检测视网膜PI3K、Akt、细胞外调节蛋白激酶(extracellular regulated kinase,ERK)、c-Jun氨基末端激酶(c-Jun N-terminal protein kainse,JNK)、丝裂原活化蛋白激酶p38抗体(p38 mitogen-activated protein kinase,p38MAPK)mRNA表达量。结果与空白对照组相比,模型组小鼠空腹血糖显著升高(P<0.01);OGTT、ITT试验各时间点血糖均升高(P<0.05,P<0.01);视网膜组织水肿,新生血管生成;视网膜葡萄糖、胰岛素含量升高(P<0.01);IRS1蛋白表达量显著升高而GLUT4蛋白表达量显著降低(P<0.01);PI3K、Akt mRNA表达量降低(P<0.05,P<0.01)、ERK、JNK、p38MAPK mRNA表达量升高(P<0.05,P<0.01)。地黄饮子和阳性对照药物均可降低小鼠OGTT、ITT试验AUC(P<0.01);改善视网膜水肿,减少新生血管;降低视网膜葡萄糖、胰岛素含量(P<0.05,P<0.05);降低小鼠视网膜IRS1蛋白表达,提高GLUT4蛋白表达(P<0.05,P<0.01)、升高PI3K、Akt mRNA表达量,降低ERK、JNK、p38MAPK mRNA表达量(P<0.05,P<0.01)。结论地黄饮子可通过激活PI3K/Akt信号通路和抑制MAPK信号通路双重调节GLUT4表达从而改善db/db小鼠视网膜胰岛素抵抗和糖代谢异常。

关键词：糖尿病视网膜病变地黄饮子胰岛素抵抗糖代谢异常双重调节

基于护理目标执行理念的护理方式在支气管哮喘患儿治疗中的应用效果研究

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广州医药 2024年第9期55卷 1078-1083页

作者：骆筱岚章秀清吴东燕陈婷婷李舒宁泉州市妇幼保健院儿童医院呼吸内科福建泉州362000

目的探讨护理目标执行理念护理在哮喘患儿治疗中的价值。方法前瞻性选取泉州市妇幼保健院于2021年8月—2023年8月收治的100例支气管哮喘患儿,应用随机数字表法将其分为两组,每组均为50例。对照组采取常规护理,观察组在常规护理基础上给... 详细信息

目的探讨护理目标执行理念护理在哮喘患儿治疗中的价值。方法前瞻性选取泉州市妇幼保健院于2021年8月—2023年8月收治的100例支气管哮喘患儿,应用随机数字表法将其分为两组,每组均为50例。对照组采取常规护理,观察组在常规护理基础上给予护理目标执行理念护理。3个月后,对比两组家属对患儿的疾病管理能力、哮喘控制状况、生活质量。结果护理后两组患儿家属相关家庭管理测量量表(FaMM)评分均升高,且观察组高于对照组(P<0.05);观察组患儿哮喘控制率高于对照组(P<0.05);护理后两组患儿情感、活动、症状相关标准儿童哮喘生活质量评分表(PAQLQ)评分均升高,且观察组高于对照组(P<0.05)。结论护理目标执行理念护理可改善哮喘患儿家属对患儿疾病的管理能力,改善哮喘控制效果,提升患儿生活质量。

关键词：护理目标执行理念支气管哮喘疾病管理

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基于运动训练的呼吸康复治疗尘肺病患者疗效meta分析

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中国职业医学 2024年第3期51卷 285-291页

作者：葛建文薛婷李志敏马星星董彦戴文娟李冬艳山西医科大学医学科学院山西太原030001 国家卫生健康委尘肺病重点实验室呼吸疾病防治与基础研究山西省重点实验室山西医科大学第一医院呼吸与危重症医学科山西太原030001

目的综合分析基于运动训练的呼吸康复治疗对职业性尘肺病(以下简称“尘肺病”)患者的疗效。方法采用文献计量学法,以中国期刊全文数据库、万方数据知识服务平台、维普数据库和中国生物医学文献服务系统为数据源,检索建库以来至2023年7... 详细信息

目的综合分析基于运动训练的呼吸康复治疗对职业性尘肺病(以下简称“尘肺病”)患者的疗效。方法采用文献计量学法,以中国期刊全文数据库、万方数据知识服务平台、维普数据库和中国生物医学文献服务系统为数据源,检索建库以来至2023年7月发表的基于运动训练的呼吸康复治疗尘肺病患者的随机对照试验文献。采用RevMan 5.4软件对入选文献进行meta分析,采用亚组分析探讨研究异质性来源,采用漏斗图法检验研究发表偏倚。结果共纳入55篇文献,涉及试验组患者2436例,对照组患者2405例。随机或固定效应模型显示,与常规治疗比较,呼吸康复治疗后患者的6 min步行距离、《健康调查简表36项》总分、用力肺活量(FVC)、第一秒用力肺活量(FEV_(1))、FEV_(1)/FVC和动脉血氧分压(PaO2)均升高(P值均<0.05),《圣乔治呼吸问卷》总分和动脉血二氧化碳分压均降低(P值均<0.05)。亚组分析结果显示,康复治疗时间≥6个月患者的《圣乔治呼吸问卷》总分、FEV_(1)和PaO2改善程度均优于<6个月者(P值均<0.01)。所纳入文献未见明显发表偏倚。结论基于运动训练的呼吸康复治疗可改善尘肺病患者的生活质量、运动耐力、呼吸功能和动脉血气。

关键词：运动训练康复尘肺病 meta分析生活质量呼吸功能

Molecular analysis of Coxsackievirus A24 variant isolates from three outbreaks of acute hemorrhagic conjunctivitis in 1988,1994 and 2007 in Beijing,China

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Virologica Sinica 2022年第2期37卷 168-176页

作者： Junhan li Fang Huang Yong Zhang Tianjiao Ji Shuangli Zhu dongyan.Wang Zhenzhi Han Jinbo Xiao Fenfen Si Wenbo Xu Dongmei Yan National Polio Laboratory WHO WPRO Regional Polio Reference LaboratoryNational Health Commission Key Laboratory for BiosecurityNational Health Commission Key Laboratory for Medical VirologyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206China Nanguan District Center for Disease Control and Prevention Changchun130042China Beijing Center for Disease Control and Prevention Beijing100000China Center for Biosafety Mega-Science Chinese Academy of SciencesWuhan430000China

Coxsackievirus A24 variant(CVA24v)is a major pathogen that causes continued outbreaks and pandemics of acute hemorrhagic conjunctivitis(AHC).In China,the first confirmed outbreak of CVA24v-related AHC occurred in Beijing in 1988,followed by another two significant outbreaks respectively in 1994 and 2007,which coincides with the three-stage dynamic distribution of AHC in the world after *** illustrate the genetic characteristics of CVA24v in different periods,a total of 23 strains were isolated from those three outbreaks and the whole genome of those isolations were sequenced and *** with the prototype strain,the 23 strains shared four nucleotide deletions in the 5'UTR except the 0744 strain isolated in *** at the 98th site,one nucleotide insertion was found in all the strains collected from *** 1994 to 2007,amino acid polarity in the VP1 region at the 25th and the 32nd site were *** the 3C and VP1 phylogenetic tree indicated that isolates from 1988 and 1994 belonged to Genotype III(GIII),and 2007 strains to Genotype IV(GIV).According to the Bayesian analysis based on complete genome sequence,the most recent common ancestors for the isolates in1988,1994 and 2007 were respectively estimated around October 1987,February 1993 and December *** evolutionary rate of the CVA24v was estimated to be 7.45×10^(-3) substitutions/site/*** study indicated that the early epidemic of CVA24v in Chinese mainland was the *** mutations and amino acid changes in different genotypes of CVA24v may generate intensity differences of the AHC ***24v has been evolving constantly with a relatively rapid rate.

关键词： Coxsackievirus A24 variant(CVA24v) Acute hemorrhagic conjunctivitis(AHC) Phylogenetic analysis Complete genome sequence

Exposure to environmental bisphenol A inhibits HTR-8/SVneo cell migration and invasion

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The Journal of Biomedical Research 2020年第5期34卷 369-378,I0007页

作者： Pu Wei Dongqing Ru Xiaoqian li dongyan.Shi Mingshun Zhang Qing Xu Hong Zhou Shuang Wen Department of Immunology Nanjing Medical UniversityNanjingJiangsu 211166China Department of Obstetrics the Affiliated Hangzhou Hospital of Nanjing Medical UniversityHangzhouZhejiang 310006China Department of Gynecology the Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical UniversityNanjingJiangsu 210004China Department of Biotherapy the Second Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210011China

Environmental pollutants,such as bisphenol A(BPA) have recently been implicated in the development of adverse birth ***,the underlying teratogenic mechanisms remain *** investigated the effects of BPA on the migration and invasion of human primary extravillous trophoblast HTR-8/SVneo *** results indicated that BPA reduced cell migration and ***,it altered the ratio of matrix metalloproteinases(MMPs) and tissue inhibitors of MMPs(TIMPs) by downregulating MMP-2 and MMP-9,and upregulating TIMP-1 and ***,BPA suppressed integrin β1,integrin α5,and ***,BPA-induced invasion was partially restored by G15,a membrane G-protein-coupled estrogen receptor 30 *** further revealed that 42 proteins were differentially expressed by mass spectrometry analysis,which could be divided into three categories based on gene ontology including biological process,cellular component,and molecular *** results suggest that BPA reduces HTR-8/SVneo cell migration and invasion by downregulating MMP-2 and MMP-9,up-regulating TIMP-1 and TIMP-2,and suppressing adhesion molecules.

关键词： bisphenol A HTR8/SVneo cells cell invasion integrinβ1

揿针结合冰硝散在踝关节骨折患者围手术期中的应用效果

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国际医药卫生导报 2024年第3期30卷 443-448页

作者：徐海霞罗海霞卓冬砚李亚伟周迎光南京中医药大学连云港附属医院连云港市中医院骨伤科连云港222004

目的观察揿针联合冰硝散在踝关节骨折患者围手术期中的应用效果。方法选取2021年12月至2023年3月连云港市中医院骨伤科踝关节骨折住院手术患者60例进行随机对照试验。采用随机数字表法将患者分为治疗组和对照组,各30例。治疗组男15例,... 详细信息

目的观察揿针联合冰硝散在踝关节骨折患者围手术期中的应用效果。方法选取2021年12月至2023年3月连云港市中医院骨伤科踝关节骨折住院手术患者60例进行随机对照试验。采用随机数字表法将患者分为治疗组和对照组,各30例。治疗组男15例,女15例,年龄(50.70±17.57)岁;患侧左16例,右14例;内踝骨折2例,外踝骨折6例,双踝骨折14例,三踝骨折8例。对照组男14例,女16例,年龄(51.87±19.43)岁;患侧左14例,右16例;内踝骨折2例,外踝骨折5例,双踝骨折16例,三踝骨折7例。所有患者均予踝关节骨折切开复位内固定术,术后常规治疗。治疗组除上述治疗外,另予揿针针刺内关、合谷穴结合冰硝散外敷,疗程1周。记录待手术天数,术后第1、7天测定患者视觉模拟量表(Visual Analogue Scale,VAS)评分、踝围、中医证候积分、局部皮肤温度、踝关节活动度及恶心呕吐发生次数。采用χ^(2)检验、t检验和Wilcoxon秩和检验。结果治疗组待手术天数少于对照组[(3.57±2.14)d比(5.00±2.08)d],差异有统计学意义(P0.05)。术后第7天,治疗组VAS评分、踝围、肤温、中医证候评分均低于对照组[(2.30±0.75)分比(3.03±0.89)分、(56.23±1.74)cm比(57.33±1.97)cm、(36.07±0.08)℃比(36.13±0.09)℃、(7.30±2.37)分比(9.07±2.15)分],跖屈角度、背屈角度均高于对照组[(28.77±1.07)°比(25.40±1.40)°、(18.30±1.95)°比(15.67±1.40)°],差异均有统计学意义(均P0.05)。结论揿针联合冰硝散能够有效减轻踝关节骨折患者术后踝部肿痛、发热症状,改善活动功能及临床症状,促进患者术后恢复,提高患者生活质量。

关键词：踝关节骨折揿针冰硝散围手术期中医护理

Interaction between diet composition and gut microbiota and its impact on gastrointestinal tract health

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Food Science and Human Wellness 2017年第3期6卷 121-130页

作者： Muhammad Shahid Riaz Rajoka Junling Shi Hafiza Mahreen Mehwish Jing Zhu Qi li dongyan.Shao Qingsheng Huang Hui Yang Key Laboratory for Space Bioscience and Space Biotechnology School of Life SciencesNorthwestern Polytechnical UniversityXi’an 710072ShaanxiPeople’s Republic of China Department of Biotechnology University of AgricultureFaisalabadPakistan

A substantial amount of emerging research is indicating that the gut microbiota has a significant impact on human *** of gut microbiota have clear consequences on intestinal homeostasis,physiology,gut microbiome,immune system and host metabolic *** composition plays an important role in the control of gut microbial populations and,thus,in the prevention,management and treatment of certain diseases such as cancer,diabetes.A comprehensive analysis of previously reported results revealed that the gut microbiota can be modulated by diet and the composition of gut microbiota can be influenced by various diet *** symbiotic relationship between different gut microbial communities regulates the immune system and,therefore,any dysbiosis can dysregulate the immune *** research is needed to fully understand the mechanisms involved in the interactions between diet composition,gut microbiota and associated diseases.

关键词： Gut microbiota Dysbiosis Probiotics Human health

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氨化椰糠基保水剂对娄土持水性能影响研究

山东化工 2024年第18期53卷 29-32页

作者：戎泽白波李小燕熊丽萍戴冬燕杨永泼浙江国际海运职业技术学院石油化工学院浙江舟山316021 长安大学旱区地下水文与生态效应教育部重点实验室陕西西安710054

本研究制备了一种氨化椰糠粉(AM/CBP)保水剂,结合室内基础试验与室内土壤环境模拟,分别以1%,5%和15%的3种用量将保水剂施入土壤中(1%,5%和15%AM/CBP-MIXED),探讨保水剂对陕西娄土物理性质及水分分布的影响。研究获得以下结论:1)经过接... 详细信息

本研究制备了一种氨化椰糠粉(AM/CBP)保水剂,结合室内基础试验与室内土壤环境模拟,分别以1%,5%和15%的3种用量将保水剂施入土壤中(1%,5%和15%AM/CBP-MIXED),探讨保水剂对陕西娄土物理性质及水分分布的影响。研究获得以下结论:1)经过接触角试验发现,制备后的保水剂亲水性提升,AM/CBP接触角为14.81°,证明其亲水性较好;2)AM/CBP能减少土壤容重,从提高土壤熟化度与总孔隙度分析。AM/CBP能增强土壤同一吸力下的含水量与饱和含水量,且这种趋势随着施加比例的增加而增加,残余含水量也符合此规律。3种比例的AM/CBP-MIXED土壤含水量增长由低到高依次为0%~193.1%,21.7%~381.7%和31.6%~604.3%;3)Gardern-Russo模型拟合能较精确地拟合AM/CBP-MIXED的土壤水分特征曲线。AM/CBP在5%的施加比例下拥有较大的田间持水量和总有效利用水,是最佳施加比例。

关键词：保水剂土壤水分土壤物理特性

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Ulva prolifera green-tide outbreaks and their environmental impact in the Yellow Sea, China

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National Science Review 2019年第4期6卷 825-838页

作者： Yongyu Zhang Peimin He Hongmei li Gang li Jihua liu Fanglue Jiao Jianheng Zhang Yuanzi Huo Xiaoyong Shi Rongguo Su Naihao Ye dongyan.liu Rencheng Yu Zongling Wang Mingjiang Zhou Nianzhi Jiao Key Laboratory of Biofuels Shandong Provincial Key Laboratory of Energy GeneticsQingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Institute of Marine Microbes and Ecospheres State Key Laboratory of Marine Environmental Science Xiamen University College of Marine Ecology and Environment Shanghai Ocean University Institute of Marine Science and Technology Shandong University Department of Oceanography Dalhousie University National Marine Hazard Mitigation Service State Oceanic Administration College of Chemistry and Chemical Engineering Ocean University of China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences State Key Laboratory of Estuarine and Coastal Research East China Normal University Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences Key Laboratory of Science and Engineering for Marine Ecology and Environment the First Institute of OceanographyState Oceanic Administration

The Ulva prolifera green tides in the Yellow Sea, China, which have been occurring since 2007, are a serious environmental problem attracting worldwide attention. Despite extensive research, the outbreak mechanisms have not been fully understood. Comprehensive analysis of anthropogenic and natural biotic and abiotic factors reveals that human activities, regional physicochemical conditions and algal physiological characteristics as well as ocean warming and biological interactions(with microorganism or other macroalgae) are closely related to the occurrence of green tides. Dynamics of these factors and their interactions could explain why green tides suddenly occurred in 2007 and decreased abruptly in ***, the consequence of green tides is serious. The decay of macroalgal biomass could result in hypoxia and acidification, possibly induce red tide and even have a long-lasting impact on coastal carbon cycles and the ecosystem. Accordingly, corresponding countermeasures have been proposed in our study for future reference in ecosystem management strategies and sustainable development policy.

关键词： green tides Ulva prolifera outbreak mechanisms eco-environmental effects Yellow Sea