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Transmission of ceftazidime-avibactam-resistant Escherichia coli among pets,veterinarians and animal hospital environment

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Biosafety and Health 2024年第3期6卷 191-198页

作者： Hegen Dai dongyan shao Yu Song Qi An Zhenbiao Zhang Haixia Zhang Siyu Chen Congming Wu Jianzhong Shen Yanli Lyu Yang Wang Shizhen Ma Zhaofei Xia National Key Laboratory of Veterinary Public Health Safety College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China Key Laboratory of Animal Antimicrobial Resistance Surveillance Ministry of Agriculture and Rural AffairsCollege of Veterinary MedicineChina Agricultural UniversityBeijing 100193China Department of Clinical Veterinary Medicine College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China Shanxi Key Laboratory for Modernization of TCVM College of Veterinary MedicineShanxi Agricultural UniversityTaiyuan 030801China Ruichen Pet Hospital Group Co. LtdXiamen 361004China

Ceftazidime-avibactam (CZA) is a recently approved combination synthetic β-lactamase inhibitor used in human clinical medicine. Cases of CZA resistance in humans have already been reported, but limited research has investigated CZA resistance in pets. This study explored the prevalence and transmission of CZA-resistant Escherichia coli (CZAREC) among pets, their owners, veterinarians, and the environment in animal hospitals. A total of 5,419 clinical samples were collected from dogs and cats, along with samples from the environment (n = 5,843), veterinarians (n = 557), and pet owners (n = 368) in animal hospitals. From these samples, 760 Escherichia coli (E. coli) isolates were obtained, out of which 60 were identified as CZAREC. These included 34 isolates from the environment (9.14 %, n = 372), three from veterinarians (8.11 %, n = 37), and 23 from animals (6.82 %, n = 337). No CZAREC isolates were found in pet owners. The predominant sequence types of CZARECs were ST156 (n = 20), ST410 (n = 19) and ST101 (n = 7). Bayesian analysis revealed six clusters comprising 47 isolates from the hospital environment, pets, and veterinaries, displaying genetic relatedness of less than 100 core genome single nucleotide polymorphisms (cgSNPs) between any two isolates in each cluster. Some CZAREC isolates with high genetic similarity persisted in the same animal hospital for four to six months. Moreover, discriminant analysis of principal components indicated that most isolates from different hosts shared a genetic source in the human/dog/cat merged cluster. Overall, evidence of CZARECs transmission was found among pets, the environment, and veterinarians in animal hospitals. The findings emphasize the importance of monitoring CZARECs in the veterinary clinical setting to ensure the health of both pets and humans.

关键词： Ceftazidime-avibactam(CZA) Escherichia coli(E.coli) Pets Animal hospital environment Veterinarians

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Genomic Shift in Population Dynamics of mcr-1-positive Escherichia coli in Human Carriage

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Genomics, Proteomics & Bioinformatics 2022年第6期20卷 1168-1179页

作者： Yingbo Shen Rong Zhang dongyan shao Lu Yang Jiayue Lu Congcong Liu Xueyang Wang Junyao Jiang Boxuan Wang Congming Wu Julian Parkhill Yang Wang Timothy R.Walsh George F.Gao Zhangqi Shen College of Veterinary Medicine China Agricultural UniversityBeijing 100193China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510641China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China The Second Affiliated Hospital of Zhejiang University School of Medicine Zhejiang UniversityHangzhou 310009China Department of Veterinary Medicine University of CambridgeCambridge CB30ESUK Department of Biology University of OxfordOxford OX13SZUK Chinese Center for Disease Control and Prevention Beijing 102206China

Emergence of the colistin resistance gene,mcr-1,has attracted worldwide *** the prevalence of mcr-1-positive Escherichia coli(MCRPEC)strains in human carriage showing a significant decrease between 2016 and 2019,genetic differences in MCRPEC strains remain largely *** therefore conducted a comparative genomic study on MCRPEC strains from fecal samples of healthy human subjects in 2016 and *** identified three major differences in MCRPEC strains between these two time ***,the insertion sequence ISApl1 was often deleted and the percentage of mcr-1-carrying IncI2 plasmids was increased in MCRPEC strains in ***,the antibiotic resistance genes(ARGs),aac(3)-IVa and blaCTX-M-1,emerged and coexisted with mcr-1 in ***,MCRPEC strains in 2019 contained more virulence genes,resulting in an increased proportion of extraintestinal pathogenic ***(ExPEC)strains(36.1%)in MCRPEC strains in 2019 compared to that in 2016(10.5%),implying that these strains could occupy intestinal ecological niches by competing with other commensal *** results suggest that despite the significant reduction in the prevalence of MCRPEC strains in humans from 2016 to 2019,MCRPEC exhibits increased resistance to other clinically important ARGs and contains more virulence genes,which may pose a potential public health threat.

关键词： Colistin mcr-1 Escherichia coli Genomics Human

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生物制剂工艺特异性残留宿主蛋白ELISA检测方法的建立及验证

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中国生物制品学杂志 2024年第2期37卷 221-226页

作者：田易晓王新月窦向雅韩翠翠高珂邵东燕段苏杨白岳丘赵雯黄庆生西北工业大学生命学院空间生物科学与生物技术重点实验室陕西西安710072

目的建立基因重组生物制剂中工艺特异性大肠埃希菌残留蛋白双抗体夹心ELISA检测方法,并进行验证。方法以大肠埃希菌表达胰高血糖素样肽(glucagon-like peptide,GLP)的生产与纯化工艺为特定工艺模型,采用同一工艺用于空载大肠埃希菌(... 详细信息

目的建立基因重组生物制剂中工艺特异性大肠埃希菌残留蛋白双抗体夹心ELISA检测方法,并进行验证。方法以大肠埃希菌表达胰高血糖素样肽(glucagon-like peptide,GLP)的生产与纯化工艺为特定工艺模型,采用同一工艺用于空载大肠埃希菌(不表达重组蛋白的正常大肠埃希菌)残留蛋白的截取。将获得的残留蛋白作为免疫原分别免疫1只雌性新西兰白兔和6只雌性昆明小鼠,以兔免疫血清纯化IgG抗体为包被抗体,鼠免疫血清为第二夹心抗体,抗鼠IgG-HRP为酶标二抗,建立工艺特异性大肠埃希菌残留蛋白双抗体夹心ELISA方法。对建立的方法进行特异性、准确性、精密性验证,并确定方法的检测限。同时采用建立的方法和市售大肠埃希菌宿主蛋白残留检测试剂盒对纯化的GLP制剂进行残留蛋白定量测定。结果对已知浓度的工艺特异性残留蛋白进行系列梯度稀释后,建立的ELISA方法检测的灵敏度可达338 pg/mL;该方法检测CHO和酵母细胞裂解蛋白无交叉反应,检测低、中、高3个浓度样品的回收率均在80%~120%范围内,检测低、中、高浓度空载大肠埃希菌截留液标准品的试验内和试验间CV均<15%。针对GLP制剂中的残留蛋白,与建立的方法检出的GLP制剂中残留蛋白总量相比,约有62%的残留蛋白未被市售非工艺特异性ELISA试剂盒检出,这些残留蛋白应为本工艺特异的残留蛋白。结论建立的双抗体夹心ELISA法检测工艺特异性大肠埃希菌残留蛋白灵敏度高,特异性强,准确性和精密性良好,可满足残留蛋白在生物制剂中低于0.01%~0.1%标准的检测要求。

关键词：工艺特异性大肠埃希菌宿主蛋白酶联免疫吸附测定

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Transmission of Carbapenem Resistance Between Human and Animal NDM-Positive Escherichia coli Strains

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Engineering 2022年第8期8卷 24-33页

作者： Yingbo Shen Fupin Hu Yongqiang Wang Dandan Yin Lu Yang Yiqiang Chen Chunyan Xu Jiyun Li Junyao Jiang Xueyang Wang Yulin Fu dongyan shao Dejun Liu Tengfei Ma Chang Cai Zhangqi Shen shaolin Wang Juan Li Rong Zhang Yuebin Ke Congming Wu Jianzhong Shen Timothy R.Walsh Yang Wang Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Institute of Antibiotics&Key Laboratory of Clinical Pharmacology of Antibiotics(MoH) Huashan HospitalFudan UniversityShanghai 200040China Department of Preventive Veterinary Medicine College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China College of Animal Science and Technology College of Veterinary MedicineZhejiang A and F UniversityHangzhou 311300China State Key Laboratory for Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseaseNational Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention(CDC)Beijing 102206China The Second Affiliated Hospital of Zhejiang University Zhejiang UniversityHangzhou 310009China Key Laboratory of Genetics&Molecular Medicine of Shenzhen Shenzhen Center for Disease Control and PreventionShenzhen 518055China Department of Zoology University of OxfordOxford OX13SZUK

Although carbapenem use is prohibited in animals in China,carbapenem-resistant Escherichia coli(CREC),especially New Delhi metallo-β-lactamase(NDM)-producing strains,are widely prevalent in foodproducing *** present,the impact of livestock-associated CREC strains on human populations at the national level is ***,we conduct a retrospective cross-sectional study to investigate the prevalence of CREC from clinical settings across 22 Chinese provinces or municipalities and analyze anthropogenic factors associated with their *** also ascertain the blaNDMand blaKPCabundance among pig and chicken farms and present a detailed genomic framework for CREC of animal and human ***,631/29799(2.1%)clinical Escherichia coli(***)isolates were identified as *** analysis revealed that being male,an age below 1,an age between 13 and 18,provinces with greater chicken production,and provinces with higher pig production were associated with higher odds of CREC *** general,73.8%(n=45/61)of pig farms and 62.2%(n=28/45)of chicken farms had a blaNDMabundance of 1×10^(-5)to 1×10^(-3)and 1×10^(-3)to 1×10^(-2),*** all the Chinese NDM-positive ***(n=463)available at the National Center for Biotechnology Information(NCBI),the genomic analysis revealed that blaNDM-5and Inc X3 were the predominant carbapenemase gene-plasmid combination,while a highly homogeneous relationship between NDM-positive isolates from humans and animals was demonstrated at the plasmid and core genome *** the findings suggest frequent CREC transmission between humans and animals,indicating that further discussions on the use of antibiotics in animals and humans are needed,both in China and across the globe.

关键词： Carbapenem resistance Escherichia coli blaNDM-5 One Health Antimicrobial resistance transmission

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Interaction between diet composition and gut microbiota and its impact on gastrointestinal tract health

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Food Science and Human Wellness 2017年第3期6卷 121-130页

作者： Muhammad Shahid Riaz Rajoka Junling Shi Hafiza Mahreen Mehwish Jing Zhu Qi Li dongyan shao Qingsheng Huang Hui Yang Key Laboratory for Space Bioscience and Space Biotechnology School of Life SciencesNorthwestern Polytechnical UniversityXi’an 710072ShaanxiPeople’s Republic of China Department of Biotechnology University of AgricultureFaisalabadPakistan

A substantial amount of emerging research is indicating that the gut microbiota has a significant impact on human *** of gut microbiota have clear consequences on intestinal homeostasis,physiology,gut microbiome,immune system and host metabolic *** composition plays an important role in the control of gut microbial populations and,thus,in the prevention,management and treatment of certain diseases such as cancer,diabetes.A comprehensive analysis of previously reported results revealed that the gut microbiota can be modulated by diet and the composition of gut microbiota can be influenced by various diet *** symbiotic relationship between different gut microbial communities regulates the immune system and,therefore,any dysbiosis can dysregulate the immune *** research is needed to fully understand the mechanisms involved in the interactions between diet composition,gut microbiota and associated diseases.

关键词： Gut microbiota Dysbiosis Probiotics Human health

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Comparison of the antitumor mechanisms of iturin A using 2D and 3D cultures of HepG2 cell

Comparison of the antitumor mechanisms of iturin A using 2D ...

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2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青年科学家论坛

作者： Haobin Zhao dongyan shao Chunmei Jiang Junling Shi 西北工业大学

To compare the results obtained in 2 D and 3 D cultures and make accurate evaluation on the antitumor potential of iturin A on HepG2 cells, both cultures were developed in this study. For 3 D culture, cells were collected to prepare cell suspension and mixed with Matrigel Matrix. The antitumor activity was tested and the mechanisms were analyzed. As results, iturin A showed significant and concentration-dependent inhibitory effect on the growth of HepG2 cells in both 2 D and 3 D cultures with IC50 of 11.80 μM and 55.26 μM, respectively. Moreover, ROS burst and DNA damage occurred in cells treated with iturin A in 2 D and 3 D cultures. The endogenous apoptosis pathway was proved to occur by Western blot analysis in 2 D and 3 D cultures. In 2 D culture, the treated cells showed a large number of vacuoles in the cytoplasm in photography. And chromatin condensation and marginalization,nuclear decomposition, ER and mitochondria swelling were observed in TEM detection. Untreated cells did not show the above change in morphology. These morphologies indicated the simultaneous occurrence of apoptosis and paraptosis in cells treated with iturin A. However, in 3 D culture, induction of paraptosis was not observed, although the induction of apoptosis and autophagy was ***, in 2 D culture, the up-regulated expression of LC 3 II and the occurrence of autophagosomes suggested the occurrence of autophagy. However, the expression of p62 was not down-regulated as expected, but up-regulated, instead. In 3 D culture, iturin A significantly increased expression of LC3 II and decreased expression of p62 and caused autophagy in iturin A-treated cells. These indicate that autophagy activity in treated cells in 2 D culture was very strong. Comparatively, 2 D culture resulted in lower IC50 and multiple pathways for the inhibitory effect of iturin A on HepG2 cells. Iturin A exhibited induction of apoptosis, autophagy, and paraptosis in 2 D culture of HepG2 cell, but only apoptosi

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黏菌素促进mcr-1阳性IncI2质粒在大肠杆菌间的接合转移

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中国农业科学 2022年第14期55卷 2862-2874页

作者：王雪杨蒋君瑶杨璐邵东延吴聪明沈建忠沈应博汪洋中国农业大学动物医学院中国科学院微生物研究所

【背景】黏菌素是人医临床治疗多重耐药革兰阴性菌感染的“最后一道防线”药物，其同时作为饲料添加剂及治疗用药长期用于畜禽养殖业。2015年，我国研究人员首次报道了质粒介导的黏菌素耐药基因mcr-1，预示该道防线面临被攻破的风险。... 详细信息

【背景】黏菌素是人医临床治疗多重耐药革兰阴性菌感染的“最后一道防线”药物，其同时作为饲料添加剂及治疗用药长期用于畜禽养殖业。2015年，我国研究人员首次报道了质粒介导的黏菌素耐药基因mcr-1，预示该道防线面临被攻破的风险。然而，杀菌浓度及亚抑菌浓度的黏菌素对mcr-1阳性质粒传播的影响仍未知。【目的】以流行最广的mcr-1阳性IncI2型质粒为研究对象，探究不同浓度黏菌素对该质粒接合转移频率的影响，为黏菌素的科学使用提供理论依据。【方法】使用肉汤法进行了不同浓度黏菌素(0.02 4μg·mL-1)作用下携带mcr-1的IncI2型质粒的接合转移试验。通过实时荧光定量PCR(RT-qPCR)及构建的公式计算不同时间点(1 24 h)的接合转移频率，并分析不同浓度黏菌素对IncI2质粒接合转移频率的影响。使用PI染料、活性氧(ROS)检测试剂盒测定不同浓度黏菌素下供体菌和受体菌的细胞膜透过性和ROS产生量。选取了对照组与低中高浓度3个处理组(0.02、1、4μg·mL-1)进行了转录组测序，使用Deseq2软件进行基因差异表达分析。采用Prism v8.2.0软件对不同组间接合转移频率、细胞膜透过性及ROS产生量的差异进行统计学分析。【结果】结合本研究建立的接合转移频率计算公式表明黏菌素浓度为4μg·mL-1时可在不同时间点提高mcr-1阳性IncI2质粒3–10倍的接合转移频率，而其他浓度则无显著差异。转录组结果显示，与对照组相比，低中高浓度黏菌素均可显著提高IncI2质粒上IV型分泌系统相关基因的表达，其中包括virB1、virB2、virB5以及traC。此外，黏菌素增加了I型菌毛合成基因fim的转录水平。PI染色结果显示当黏菌素浓度为2和4μg·mL-1时，可增强供体菌和受体菌的细胞膜透过性，且转录组结果也显示膜相关基因(ompAX、bamDE、lolB、yiaD、csgEF)的转录水平均出现显著上调。但是黏菌素作用后ROS产生量及相关基因的转录水平无显著提高。【结论】揭示了黏菌素可通过增加细菌IV型分泌系统活性、细胞膜透过性和菌毛的生成从而提高mcr-1阳性IncI2质粒在大肠杆菌间的接合转移频率。研究结果提示，杀菌浓度无法完全杀死mcr-1阳性大肠杆菌的同时，还能增加质粒传播速度。因此，畜禽养殖业中黏菌素的治疗使用可能维持mcr-1阳性质粒的存在及传播，此外鉴于黏菌素已批准用于人医临床，该现象有可能引起临床黏菌素治疗mcr-1阳性病原菌的失败。

关键词：黏菌素 mcr-1 大肠杆菌 IncI2质粒接合转移

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西藏“灵菇”乳的发酵与抑菌机制研究

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农业工程学报 2008年第7期24卷 269-272页

作者：邵东燕杨保伟师俊玲毋锐琴张学良西北农林科技大学食品学院杨凌712100

根据发酵过程中菌粒中主要微生物数量与发酵乳中主要营养物和产物含量间的关系,分析探讨了西藏"灵菇"乳的发酵机制;考察了不同发酵时期所得发酵乳对大肠杆菌、金黄色葡萄球菌、沙门氏菌的抑菌活性,结合化学物质模拟试验揭示了发酵乳的... 详细信息

根据发酵过程中菌粒中主要微生物数量与发酵乳中主要营养物和产物含量间的关系,分析探讨了西藏"灵菇"乳的发酵机制;考察了不同发酵时期所得发酵乳对大肠杆菌、金黄色葡萄球菌、沙门氏菌的抑菌活性,结合化学物质模拟试验揭示了发酵乳的抑菌机制。结果显示,发酵前期,菌粒中的各微生物由于向乳中扩散及生长延滞,数量稍有减少;随着发酵的进行,菌粒中乳酸菌和酵母菌的数量一直处于相对稳定的水平,但醋酸菌含量呈明显的先升后降趋势;在整个发酵过程中,菌粒中的乳酸菌数量一直处于最高水平,其次为酵母菌,醋醋菌数量最少;乳酸是西藏"灵菇"发酵乳中的主要抑菌活性物质;乙酸和乙醇的存在对低浓度乳酸的抑菌活性有增效作用,但当乳酸浓度较高时,这种增效作用减弱。西藏"灵菇"发酵乳的总抑菌效果是微生物与其代谢产物的共同作用结果。

关键词：西藏“灵菇” 菌粒发酵牛乳抑菌活性

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聚酰亚胺阳离子染料载体染色性能

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高分子材料科学与工程 2019年第4期35卷 52-56页

作者：邵冬燕林怡漫杜金梅许长海王鸿博江南大学纺织服装学院生态纺织教育部重点实验室江苏无锡214122

以阳离子蓝SD-GSL染料和载体PEA在高温高压下对聚酰亚胺织物进行染色,研究高性能聚酰亚胺纤维的染色性能。通过正交实验设计对载体、电解质和pH值3个染色影响因素进行了研究。结果表明,载体和电解质的加入可以提高聚酰亚胺的染色性能,染... 详细信息

以阳离子蓝SD-GSL染料和载体PEA在高温高压下对聚酰亚胺织物进行染色,研究高性能聚酰亚胺纤维的染色性能。通过正交实验设计对载体、电解质和pH值3个染色影响因素进行了研究。结果表明,载体和电解质的加入可以提高聚酰亚胺的染色性能,染液pH值对染色效果影响较小。聚酰亚胺纤维在含有载体和电解质的染色体系染色后具有良好的耐洗和耐摩擦色牢度,染料与纤维原有金黄色遵循减法混色原则,染色后纤维的颜色得以改变。扫描电镜测试结果表明,高温高压载体染色对纤维的表观形貌未产生明显的影响;热重分析表明经载体染色后聚酰亚胺纤维保持了良好的热稳定性;聚酰亚胺纱线的强力拉伸断裂测试结果显示纤维的强力有所下降。

关键词：聚酰亚胺阳离子染料载体电解质染色

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应用阳离子漂白活化剂的棉织物快速轧蒸漂白工艺

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纺织学报 2019年第2期40卷 125-129页

作者：唐文君彭明华向中林邵冬燕倪佳东许长海生态纺织教育部重点实验室(江南大学) 江苏无锡214122 江苏联发纺织股份有限公司江苏省生态染整技术重点实验室江苏南通226601

为提高棉织物漂白工艺效率,降低能耗,使用阳离子漂白活化剂N-(4-(三乙基铵甲撑)苯酰基)己内酰胺氯化物(TBCC)构建棉机织物的快速轧蒸漂白体系,对精练棉机织物进行轧蒸前处理。通过测试快速轧蒸漂白织物的白度和聚合度分析了碱剂、TBCC... 详细信息

为提高棉织物漂白工艺效率,降低能耗,使用阳离子漂白活化剂N-(4-(三乙基铵甲撑)苯酰基)己内酰胺氯化物(TBCC)构建棉机织物的快速轧蒸漂白体系,对精练棉机织物进行轧蒸前处理。通过测试快速轧蒸漂白织物的白度和聚合度分析了碱剂、TBCC的质量浓度、汽蒸时间以及双氧水稳定剂用量对漂白效果的影响。结果表明,使用柠檬酸钠为pH调节剂,并控制TBCC、双氧水、柠檬酸钠的量比为1∶1.2∶1.4,可使快速轧蒸漂白工艺的漂白性能达到最佳。所构建的快速轧蒸漂白工艺可将汽蒸时间缩短至4 min以内,使棉机织物的CIE白度值由36.30%提高至80.65%,而织物纤维并没有受到显著损伤。

关键词：阳离子漂白活化剂轧蒸活化漂白棉机织物

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