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Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus

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Journal of Oceanology and Limnology 2024年第05期 1673-1682页

作者： dongxiu xue Sudong XIA Tengfei XING Yuqian GAO Jinxian LIU CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences Laboratory for Marine Ecology and Environmental Science Qingdao Marine Science and Technology Center Tianjin Key Lab of Aqua-Ecology and Aquaculture Department of Fishery Science

The mechanisms underlying sex determination and differentiation have long intrigued researchers in the fields of development and evolutionary biology. The roughskin sculpin(Trachidermus fasciatus Heckel), displaying sexual dimorphism, provides an ideal model for studying the ***, both genetic and genomic information concerning sex determination and differentiation, such as gonadal transcriptome data in roughskin sculpin, are lacking. Here, we present the first gonadal transcriptomes of roughskin sculpin and identify sex-related genes. We identified 8 531 differentially expressed genes(DEGs), among them 4 065 were upregulated in the ovary and 4 466 upregulated in the testis. Several sex-related gene ontology(GO) terms were enriched in ovary-biased genes, including “binding of sperm to zona pellucida”, “egg coat formation”, “positive regulation of acrosome reaction”,“cell division”, and “cell cycle”, while the GO terms such as “spermatogenesis”, “sperm axoneme assembly”, “cilium assembly”, “cilium movement”, and “cilium movement involved in cell motility”were enriched in testis-biased genes. Moreover, six KEGG pathways were significantly enriched in the ovary, whereas only one was enriched in the testis. Of these DEGs, 40 sex-related genes were identified,which including 26 testis-biased genes(such as Dmrtb1, Gsdf, Sox9b, Wnt4b, Tcp11l2, and Efhb), and 14ovary-biased genes(such as Cyp19a1a, Foxh1, Foxr1, Gdf3, Hsd17b12, and Igf2bp3). This gonadal transcript dataset would broaden our understanding of sex determination and differentiation mechanisms in roughskin sculpin, expand the genomic database, support future studies on sex-related gene functions,and facilitate molecular biology research into roughskin sculpin.

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中国近海栉江珧属分类与地理分布研究进展

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海洋科学 2024年第4期48卷 122-129页

作者：薛东秀王海艳马兴浩张涛中国科学院海洋研究所山东青岛266071 寿光市海洋渔业发展中心山东潍坊262700

本文收集了涉及中国栉江珧属(Atrina Gray,1842)分类和分布的文献,结合近年来栉江珧属分子生物学的研究结果以及野外调查情况,对栉江珧属种类和地理分布情况进行了系统的梳理,确定了中国共有7种栉江珧属贝类,并对其分类分歧进行了讨论;... 详细信息

本文收集了涉及中国栉江珧属(Atrina Gray,1842)分类和分布的文献,结合近年来栉江珧属分子生物学的研究结果以及野外调查情况,对栉江珧属种类和地理分布情况进行了系统的梳理,确定了中国共有7种栉江珧属贝类,并对其分类分歧进行了讨论;概括各栉江珧属种类的地理分布,广东是中国近海栉江珧属物种多样性最丰富的省份,其次为广西壮族自治区。根据目前中国栉江属种类的资源现状,对栉江珧属种类的开发利用与保护提出了建议,以期为深入开展全国范围的栉江珧属生物多样性保护和可持续利用提供科学依据。

关键词：栉江珧属(Atrina Gray,1842) 物种分类地理分布资源保护

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Genetic variation within and among range-wide populations of three ecotypes of the Japanese grenadier anchovy Coilia nasus with implications to its conservation and management

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Journal of Oceanology and Limnology 2020年第3期38卷 851-861页

作者： xue dongxiu YANG Qiaoli ZONG Shaobing GAO Tianxiang LIU Jinxian Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China School of Fisheries Zhejiang Ocean UniversityZhoushan 316022China

Studies of range-wide populations can contribute to the comprehension of the relative roles of historical events and contemporary factors that influence genetic variation within and among *** grenadier anchovy,Coilia nasus,is a commercially important fish,which widely distributes in the Changjiang River,the coasts of China and Korea,and the Ariake Sea of *** species exhibits three life-history strategies(anadromous,landlocked and freshwater resident forms).Using two fragments of nuclear DNA,genetic variation within and among 18 populations across the rivers and coast of China and Ariake Sea of Japan was *** of genetic diversity and divergence among populations varied widely across ***'range,and indicated the different effects of historical events and anthropogenic *** genetic divergence between freshwater resident populations and other populations suggested that historical geographical factors greatly influenced the genetic structure of *** genetic differentiation observed among lakes in lower Changjiang River and Huaihe River might be probably influenced by hydraulic *** population genetic structure among the three ecotypes revealed in the present study indicated an important role for environment variation,and the factors responsible for shaping *** different life history strategies might also impact population structure.

关键词： Japanese grenadier anchovy population structure anthropogenic factors life-history nuclear DNA sequences

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Ultrastructure Developments During Spermiogenesis in Polydora ciliata(Annelida: Spionidae),a Parasite of Mollusca

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Journal of Ocean University of China 2014年第6期13卷 1071-1077页

作者： GAO Yan ZHANG Tao ZHANG Libin QIU Tianlong xue dongxiu YANG Hongsheng Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of Sciences University of Chinese Academy of Sciences

Spionid worms of Polydora ciliata inhabit the shells of many commercially important bivalves and cause disease in molluscan aquaculture. Their sperm structure is closely related to their fertilization method. To give an insight into the sperm structure and spermatogenesis, ultrastructure details of the subcellular components of germ cells during spermiogenesis of Polydora ciliata are detected by transmission electron microscopy(TEM). In P. ciliata, during spermiogenesis, chromatin is regularly arranged as dense fibrils and becomes more condensed when the nucleus elongates. Microtubules do not surround the nucleus during its elongation. The Golgi phase is characterized by the formation of proacrosomal granules within the Golgi apparatus. The proacrosomal granules fuse to form a single, spherical acrosomal vesicle that migrates to the anterior pole of the cell. At the time of nuclear condensation, mitochondria become reduced in number but increased in size, causing deep indentation at the base of the nucleus. The mid-piece has a few mitochondria. The cap phase includes the spreading of the acrosomal granule over the surface of the nucleus of the differentiating spermatid. The acrosomal phase of spermiogenesis is typically associated with changes in the shape of the nucleus, acrosome and tail. The relationship of sperm ultrastructure to spermiogenesis in spionidae species was discussed.

关键词： Polydora ciliata spermiogenesis ultrastructure

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某老图书馆改造产生墙体裂缝的分析及处理

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贵州大学学报（自然科学版） 2000年第3期17卷 230-233页

作者：郭峰宋冬秀薛朝霞贵州大学建筑工程系贵阳550025

通过对某老图书馆改造过程中产生墙体裂缝的观察 ,原因的分析及处理。

关键词：房屋改造墙体裂缝处理图书馆砖混结构

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水中痕量铜的中性红-曙红Y同步荧光猝灭法检测

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分析仪器 2014年第6期 24-29页

作者：肖锡林薛金花廖力夫贺冬秀王芳袁金斌何博南华大学化学化工学院南华大学公共卫生学院衡阳421001 江西中医学院现代中药制剂教育部重点实验室南昌330004

在pH8.5的Tris-HCl缓冲液中,曙红Y-中性红和Cu(Ⅱ)相互作用,在538nm处随着Cu(Ⅱ)浓度的增加,体系同步荧光强度逐渐降低,同步荧光强度降低值和Cu(Ⅱ)含量在一定范围内有相关关系。据此应用中性红-曙红Y同步荧光猝灭法建立一种测定铜的新... 详细信息

在pH8.5的Tris-HCl缓冲液中,曙红Y-中性红和Cu(Ⅱ)相互作用,在538nm处随着Cu(Ⅱ)浓度的增加,体系同步荧光强度逐渐降低,同步荧光强度降低值和Cu(Ⅱ)含量在一定范围内有相关关系。据此应用中性红-曙红Y同步荧光猝灭法建立一种测定铜的新方法。在优化的实验条件下,体系在538nm处的荧光强度降低值与铜的摩尔浓度在7.61×10-8-3.60×10-6 mol·L-1范围内线性关系良好,其线性回归方程为:△F=-0.414+314.89c(×10-6 mol·L-1),相关系数r=0.998,检出限为2.48×10-8 mol·L-1,相对标准偏差是0.26%,加标回收率是101.12%。本方法操作简便、灵敏、选择性好,可用于实际样品中铜的测定。

关键词：中性红铜同步荧光猝灭

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影响河北地区男性精液质量因素的初步分析

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中国性科学 2022年第7期31卷 24-27页

作者：许鹏宇程立立陈超麻庆玲郭瑞娟王雪李怡李冬秀河北医科大学第三医院生殖医学科石家庄050051 河北省生殖医院男科石家庄050000 广州市妇女儿童诊疗中心检验科广州510623

目的探讨河北地区男性精液质量的影响因素,为当地男性提供科学合理的健康指导。方法以调查问卷形式调查门诊男性患者的年龄、体重指数(BMI)、吸烟、饮酒等情况,并进行精液检测。结果年龄与精液量、精子总数、前向运动精子率(PR)、正常... 详细信息

目的探讨河北地区男性精液质量的影响因素,为当地男性提供科学合理的健康指导。方法以调查问卷形式调查门诊男性患者的年龄、体重指数(BMI)、吸烟、饮酒等情况,并进行精液检测。结果年龄与精液量、精子总数、前向运动精子率(PR)、正常形态精子率呈负相关,与精子DNA碎片率(DFI)呈正相关(P0.05)。结论年龄、吸烟、饮酒、肥胖是引起河北地区男性精液质量下降的主要因素,备孕男性应改善不良生活习惯,提高精液质量。

关键词：男性生育力不良生活习惯吸烟饮酒肥胖

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