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Overexpression of C-terminal fragment of glutamate receptor 6 prevents neuronal injury in kainate-induced seizure via disassembly of Glu R6-PSD95-MLK3 signaling module

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Neural Regeneration Research 2014年第23期9卷 2059-2065页

作者： Jie Mou Xiaomei Liu dongsheng pei Jiangsu Key Laboratory of Targeted Drug and Clinical Application Xuzhou Medical College School of Basic Medical Science Xuzhou Medical College Jiangsu Key Laboratory of Biological Cancer Therapy Xuzhou Medical College

Our previous study showed that when glutamate receptor （GluR）6 C terminus-containing peptide conjugated with the human immunodeficiency virus Tat protein （GluR6）-9c is delivered into hippocampal neurons in a brain ischemic model, the activation of mixed lineage kinase 3 （MLK3） and c-Jun NH2-terminal kinase （JNK） is inhibited via GluR6-postsynaptic density protein 95 （PSD95）. In the present study, we investigated whether the recombinant adenovirus （Ad） carrying GluR6c could suppress the assembly of the GluR6-PSD95-MLK3 signaling module and decrease neuronal cell death induced by kainate in hippocampal CA1 subregion. A seizure model in Sprague-Dawley rats was induced by intraperitoneal injections of kainate. The effect of Ad- Glur6-9c on the phosphorylation of INK, MLK3 and mitogen-activated ldnase kinase 7 （MKK7） was observed with western immunoblots and immunohistochemistry. Our findings revealed that overexpression of GluR6c inhibited the interaction of GluR6 with PSD95 and prevented the kainate-induced activation of INK, MLK3 and MKK7. Furthermore, kainate-mediated neuronal cell death was significantly suppressed by GluR6c. Taken together, GluR6 may play a pivotal role in neuronal cell death.

关键词： nerve regeneration brain injury hippocampal neuronal injury seizures adenovirus GluR6 PSD95 MLK3 kainate apoptosis JNK NSFC grants neural regeneration

Circular RNA ubiquitin-associated protein 2 enhances autophagy and promotes colorectal cancer progression and metastasis via miR-582-5p/FOXO1 signaling

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Journal of Genetics and Genomics 2021年第12期48卷 1091-1103页

作者： Feifei Chen Lei Guo Jiehui Di Man Li Dong Dong dongsheng pei Department of Cell Biology Xuzhou Medical UniversityXuzhouJiangsu 221002China Cancer Institute Xuzhou Medical UniversityXuzhouJiangsu 221002China

Numerous circular RNAs(circ RNAs) have been identified as vital regulators in various cancers. The newly reported circular RNA ubiquitin-associated protein 2(circ UBAP2) is a critical player in cell growth and metastasis in various types of cancers, although its role in colorectal cancer(CRC) has yet to be fully elucidated. We find that circ UBAP2 is upregulated in CRC tissues and cell lines to induce autophagy both in vitro and in vivo. The effects of circ UBAP2 on migration, invasion, and proliferation may be partially related to autophagy. Mechanistically, we uncover that circ UBAP2 can directly interact with mi R-582-5 p and subsequently act as a micro RNA sponge to regulate the expression of the mi R-582-5 p target gene forkhead box protein O1(FOXO1) and downstream signaling molecules, which collectively advance the progression and metastasis of CRC. These results suggest that circ UBAP2 acts as an oncogene via a novel circ UBAP2/mi R-582-5 p/FOXO1 axis, providing a potential biomarker and therapeutic target for CRC management.

关键词： CircUBAP2 MiR-582-5p FOXO1 Autophagy Colon cancer

CSN6 promotes tumorigenesis of gastric cancer by ubiquitin-independent proteasomal degradation of p16INK4a

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Cancer Biology & Medicine 2019年第3期16卷 514-529页

作者： Wenqi Du Zongxiang Liu Wentao Zhu Tongtong Li Zhiman Zhu Lulu Wei Jun Song dongsheng pei Department of Pathology Xuzhou Medical UniversityXuzhou221004China Department of Anatomy Xuzhou MedicalUniversityXuzhou221004China Department of Periodontal Mucosal Diseases the Affiliated Xuzhou StomatologicalHospital of Xuzhou Medical UniversityXuzhou221002China Department of General Surgery the Affiliated Hospital ofXuzhou Medical UniversityXuzhou221002China Institute of Digestive Diseases Xuzhou Medical UniversityXuzhou221002China

Objective: CSN6 is a vital subunit of the constitutive photomorphogenesis 9(COP9) signalosome(CSN), which is responsible for development disorders and promotes ubiquitin-26 S proteasome-dependent degradation in vitro and vivo.Its role in the tumor development of gastric cancer remains unclear.In this study, we investigated the role of CSN6 in gastric cancer progression.Methods: Human gastric cancer samples were collected and immunohistochemistry was performed to identify the role of CSN6 in gastric cancer.The cell proliferation was measured by CCK-8 and the EdU incorporation method.Immunofluorescence localization and a co-immunoprecipitation study were used to show the interaction between the protein CSN6 and p16.Ubiquitination assay was performed to validate whether ubiquitination is involved in CSN6-mediated p16 degradation.BALB/c nude mice were used to produce a tumor model in order to test the effect of CSN6 on cancer growth in vivo.Results: CSN6 expression was dramatically increased in gastric cancer tissues compared with paired adjacent non-tumor tissues and CSN6 was correlated with worse overall and disease-specific survival.Additionally, we also found that CSN6 downregulated p16 protein expression, thereby promoting gastric cancer cell growth and proliferation.Moreover, CSN6 interacted with p16 and a proteasome activator REGγ(PA28γ), thereby facilitating ubiquitin-independent degradation of p16.Conclusions: CSN6 promoted the loss of p16-mediated tumor progression and played an important role in regulating ubiquitin-independent proteasomal degradation of p16.

关键词： CSN6 gastric cancer proliferation p16 REGγ

Ultrathin ternary PtNiGa nanowires for enhanced oxygen reduction reaction

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Chinese Chemical Letters 2024年第2期35卷 599-603页

作者： Giday Fisseha Yiping Hu Yanan Yu Shaojie Lu dongsheng Ma pei Nian Zheng Wang Qin Yue Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 610054China State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering School of Chemistry and Chemical EngineeringNingxia UniversityYinchuan 750021China

Achieving high activity and durability for the oxygen reduction reaction(ORR)with an ultra-low amount of platinum is significant to promote the widespread application of proton exchange membrane fuel cells(PEMFCs).Here we report a new ultrathin(∼1 nm)ternary PtNiGa alloy nanowires(PtNiGa NWs)electrocatalyst,in which the presence of gallium(Ga)enhances the oxidation resistance of platinum(Pt)and nickel(Ni)and suppresses the dissolution of Ni.The mass and specific activities of PtNiGa NWs are about 11.2 and 7.6 times higher than those of commercial Pt/C catalysts for ORR.Moreover,the mass activity of PtNiGa/C NWs nanocatalyst decreased only by 12.8%and largely retained its electrochemical surface area(ECSA)after 10,000 potential cycles,compared with 38%loss of ECSA for commercial Pt/C catalyst.Therefore,this work provides a general guideline for preparing ternary alloy electrocatalysts and enhancing the activity and stability of the cathode ORR reaction of PEMFCs.

关键词： Ultrathin nanowires Gallium Oxygen reduction reaction Ternary alloy Durability

“六艺之教”:马一浮《论语》诠释的教化维度

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中国哲学史 2023年第5期 85-93页

作者：裴东升山东大学儒学高等研究院

马一浮以“六艺论”自觉挺立了儒学的教化维度。马一浮非常重视《论语》,并将其置于“六艺之教”的观照下以显现“大义”。他认为《论语》处处都体现了“六艺”大旨:《论语》有“问仁”“问政”“问孝”三大问目,其中凡答问仁都是“‘... 详细信息

马一浮以“六艺论”自觉挺立了儒学的教化维度。马一浮非常重视《论语》,并将其置于“六艺之教”的观照下以显现“大义”。他认为《论语》处处都体现了“六艺”大旨:《论语》有“问仁”“问政”“问孝”三大问目,其中凡答问仁都是“‘诗’教”义,答问政都是“‘书’教”义,答问孝为“‘礼乐’教”义。又“‘易’教”是“礼乐教”之原,因而“‘礼乐’教”即蕴涵“‘易’教”;“‘春秋’教”是“‘诗’教”“‘书’教”之用,因而“‘诗’教”“‘书’教”中自然有“‘春秋’教”。这一诠释进路的意义在于:一是将《论语》融摄于“六艺之教”,继承并贯彻了儒学“教化”观念,使“六经”在儒学现代化这一学术视野中依然可以“在场”。二是重新绾合“六经”和《论语》在本源意义上的联系,为“六经”是孔子之教提供了极为有力的佐证。

关键词：马一浮论语教化六艺六经

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直接空冷乏汽提质供热系统变工况特性研究

浙江电力 2023年第11期42卷 122-128页

作者：邢岳谭浩艺裴东升王东波范诚豪安徽淮南平圩发电有限责任公司安徽淮南232000 华能(上海)电力检修有限责任公司上海200942 中国电力工程顾问集团华北电力设计院有限公司北京100120 上海发电设备成套设计研究院有限责任公司上海200240

直接空冷机组高背压供热方式受热网循环水回水温度和空冷岛换热单元协调控制的限制,运行经济性和发电出力两者之间难以平衡。为解决上述矛盾,采用商业软件Ebsilon Professional对直接空冷乏汽提质供热系统在不同负荷下的空冷岛背压、汽... 详细信息

直接空冷机组高背压供热方式受热网循环水回水温度和空冷岛换热单元协调控制的限制,运行经济性和发电出力两者之间难以平衡。为解决上述矛盾,采用商业软件Ebsilon Professional对直接空冷乏汽提质供热系统在不同负荷下的空冷岛背压、汽轮机出力、汽轮机热耗和喷射器引射比等关键性能进行了变工况计算和分析。计算结果表明,乏汽提质供热系统在冬季低负荷时最佳运行背压为13~20 kPa,可以实现供热能量的梯级利用和机组出力的平衡,并满足防冻安全的运行要求。

关键词：乏汽提质供热直接空冷变工况特性

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苯并咪唑衍生物的抗肿瘤活性及构效关系研究

中国医药工业杂志 2021年第3期52卷 283-298页

作者：牟杰陈宁海裴冬生江苏省新药研究与临床药学重点实验室徐州医科大学药学院

恶性肿瘤是严重威胁人类生命健康的疾病,随着其发病率和死亡率的不断上升,对抗肿瘤药物的开发提出了巨大的需求。作为核酸类似物的苯并咪唑衍生物,具有广谱的抗肿瘤活性,是抗肿瘤药物的优势骨架。近年来研究表明苯并咪唑衍生物对肝癌、... 详细信息

恶性肿瘤是严重威胁人类生命健康的疾病,随着其发病率和死亡率的不断上升,对抗肿瘤药物的开发提出了巨大的需求。作为核酸类似物的苯并咪唑衍生物,具有广谱的抗肿瘤活性,是抗肿瘤药物的优势骨架。近年来研究表明苯并咪唑衍生物对肝癌、肺癌、乳腺癌、肾癌、前列腺癌、宫颈癌等多种肿瘤细胞有良好的抗肿瘤效果。本文综述了苯并咪唑类衍生物与肿瘤靶点的构效关系,为设计新型分子靶向抗肿瘤药物提供了参考和依据。

关键词：苯并咪唑抗肿瘤活性构效关系靶点

过氧化氢通过调控自噬增强宫颈癌的放疗敏感性

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山东大学学报（医学版） 2023年第6期61卷 22-28页

作者：任慧欣郑茂金韩文灿王超群周云裴冬生徐州医科大学临床与实验病理学实验室江苏徐州221004 徐州医科大学徐州临床学院放疗科江苏徐州221004

目的探讨过氧化氢(H_(2)O_(2))联合透明质酸钠是否可以提高宫颈癌细胞的放疗敏感性,并探讨其潜在机制。方法将细胞分为对照组、透明质酸钠组、4 Gy组、透明质酸钠+4 Gy组,Western blotting实验检测自噬相关蛋白水平。将细胞分为对照组,... 详细信息

目的探讨过氧化氢(H_(2)O_(2))联合透明质酸钠是否可以提高宫颈癌细胞的放疗敏感性,并探讨其潜在机制。方法将细胞分为对照组、透明质酸钠组、4 Gy组、透明质酸钠+4 Gy组,Western blotting实验检测自噬相关蛋白水平。将细胞分为对照组,透明质酸钠组,200μmol/L H_(2)O_(2)组、500μmol/L H_(2)O_(2)组、700μmol/L H_(2)O_(2)组和1000μmol/L H_(2)O_(2)组,CCK8实验检测H_(2)O_(2)对细胞活力的影响;将细胞分为对照组和200μmol/L H_(2)O_(2)组,分别进行0、2、4、6、8 Gy放疗,克隆形成实验评估H_(2)O_(2)的放疗增敏作用。将细胞分为对照组、200μmol/L H_(2)O_(2)组、4 Gy组、200μmol/L H_(2)O_(2)+4 Gy组,Western blotting实验和免疫荧光实验检测自噬相关蛋白水平。结果对照组与透明质酸钠组的自噬相关蛋白LC3 II、p62表达差异无统计学意义(P>0.05);4 Gy组与透明质酸钠+4 Gy组的自噬相关蛋白LC3 II、p62表达差异无统计学意义(P>0.05)。CCK8实验结果表明,放疗前后,对照组和透明质酸钠组的细胞增殖率差异无统计学意义(P>0.05);与对照组相比,200μmol/L H_(2)O_(2)组促进细胞增殖(P0.05),700μmol/L H_(2)O_(2)组和1000μmol/L H_(2)O_(2)组抑制细胞增殖(P<0.05)。克隆形成实验结果表明,H_(2)O_(2)增加了细胞对放疗的敏感性(P<0.05)。Western blotting实验和免疫荧光实验表明,与对照组相比,200μmol/L H_(2)O_(2)组抑制了自噬(P<0.05);与4 Gy组相比,200μmol/L H_(2)O_(2)+4 Gy组促进了自噬(P<0.05)。与对照组相比,200μmol/L H_(2)O_(2)组p-AKT和p-mTOR蛋白表达增加(P<0.05);与4 Gy组相比,200μmol/L H_(2)O_(2)+4 Gy组p-AKT和p-mTOR的表达减少(P<0.05)。结论H_(2)O_(2)通过调控AKT/mTOR通路进而调控自噬来提高宫颈癌的放疗敏感性。

关键词：自噬过氧化氢透明质酸钠放疗敏感性 AKT/mTOR通路宫颈癌

广元市某医院2016-2020年受血者病原体感染调查及防控建议

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医学动物防制 2024年第3期40卷 296-299页

作者：王大方石平郑小黎钟冬胜梁培广元市中心医院四川广元628017 海南医学院

目的了解广元市某医院2016-2020年8 238例受血者病原体感染情况,并进一步探讨后期防控计划。方法按年份及性别调查受血者输血前的乙型肝炎表面抗原(hepatitis B surface antigen, HBsAg)、苍白密螺旋体抗体(Treponema pallidum antibo... 详细信息

目的了解广元市某医院2016-2020年8 238例受血者病原体感染情况,并进一步探讨后期防控计划。方法按年份及性别调查受血者输血前的乙型肝炎表面抗原(hepatitis B surface antigen, HBsAg)、苍白密螺旋体抗体(Treponema pallidum antibody, TP-Ab)、人类免疫缺陷病毒抗体(Human immunodeficiency virus antibody, HIV-Ab)、丙型肝炎病毒抗体(Hepatitis C virus antibody, HCV-Ab)检测结果。结果 2016-2020年该院8 238例受血者,检测指标总阳性率13.66%,HBsAg、TP-Ab、HIV-Ab、HCV-Ab的阳性率分别为10.50%、1.24%、0.04%、1.88%。不同性别人群4项传染指标的阳性率差异无统计学意义(χ^(2)=0.314、5.054、3.000、0.830,P>0.05)。2016-2020年HBsAg的年阳性率分别为11.93%、11.74%、10.69%、9.36%、8.92%,TP-Ab的年阳性率分别为1.40%、1.12%、1.02%、1.35%、1.30%,HIV-Ab的年阳性率分别为0%、0.06%、0.06%、0%、0.06%,HCV-Ab的年阳性率分别为1.91%、1.82%、2.04%、1.79%、1.86%,不同年份的HBsAg阳性率差异有统计学意义(χ^(2)=13.236,P=0.010),不同年份的TP-Ab、HIV-Ab、HCV-Ab阳性率差异均无统计学意义(χ^(2)=1.410、1.910、0.333,均P=0.842、0.752、0.988)。结论广元市某医院2016-2020年受血者的病原体感染率较低,在防控HBV感染时,需进一步完善硬件保障,可建立专项传染病院,规范传染科病房,确保传染病的及时治疗。

关键词：受血者病原体感染防控建议调查