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miR-924、SLC1A5在肺癌组织中的表达及其在预后评估中的价值

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检验医学 2024年第1期39卷 13-18页

作者：张苗苗段东奎余春王丽娜南阳市中心医院胸外科肺部病区河南南阳473000

目的探讨miR-924和溶质载体家族1成员5(SLC1A5)在肺癌患者预后评估中的价值。方法选取2018年2月—2019年2月南阳市中心医院肺癌患者97例,收集所有患者的临床资料,并收集癌组织和癌旁组织(距肿瘤边缘>2 cm),检测miR-924、SLC1A5 mRNA和... 详细信息

目的探讨miR-924和溶质载体家族1成员5(SLC1A5)在肺癌患者预后评估中的价值。方法选取2018年2月—2019年2月南阳市中心医院肺癌患者97例,收集所有患者的临床资料,并收集癌组织和癌旁组织(距肿瘤边缘>2 cm),检测miR-924、SLC1A5 mRNA和SLC1A5蛋白表达。采用Pearson相关分析评估miR-924与SLC1A5 mRNA的相关性。采用Kaplan-Meier生存曲线评估肺癌患者的生存情况。采用Cox回归分析评估肺癌患者预后不良的危险因素。结果与癌旁组织比较,癌组织miR-924相对表达量降低(P<0.001),SLC1A5mRNA相对表达量升高(P<0.001)。癌组织SLC1A5蛋白阳性率显著高于癌旁组织(P<0.001)。癌组织miR-924表达与SLC1A5 mRNA表达呈负相关(r=-0.843,P<0.05)。根据癌组织miR-924相对表达量均值或SLC1A5蛋白的表达情况分别分为高表达组、低表达组和阳性组、阴性组。miR-924低表达组与高表达组之间、SLC1A5阳性组与阴性组之间分化程度、临床分期差异均有统计学意义(P<0.05)。Kaplan-Meier生存曲线分析结果显示,miR-924高表达组累积生存率高于低表达组(Log-rankχ^(2)=5.453,P<0.05);SLC1A5阳性组累积生存率低于阴性组(Log-rankχ^(2)=9.259,P<0.05)。多因素Cox回归分析结果显示,临床分期Ⅲ期、miR-924低表达、SLC1A5蛋白表达阳性均是肺癌患者预后不良的危险因素(P<0.05)。结论肺癌患者癌组织mi R-924和SLC1A5均呈异常表达,或可作为肺癌预后评估的生物标志物。

关键词：微小RNA-924 溶质载体家族1成员5 肺癌预后

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沙田柚黄龙病近红外光谱检测研究

中国南方果树 2021年第3期50卷 9-12页

作者：刘福平王茜陈东奎廖惠红黄宏明张尧良黄其椿汪妮娜张兰董伯年何东模施平丽广西壮族自治区农业科学院园艺研究所/农业部南宁南亚热带果树科学观测实验站/广西柑桔黄龙病防控工程技术研究中心南宁530007 广西壮族自治区容县农业科学研究所广西容县537500

2019年从容县各乡镇轻度感病、中度感病、重度感病以及未感病但有其他黄化症状的305株沙田柚树上,采集了590份不同症状的叶片样品,在Nested-PCR检测的基础上,使用广州讯动网络有限公司开发的手持式近红外光谱仪采集光谱,建立标准数据库... 详细信息

2019年从容县各乡镇轻度感病、中度感病、重度感病以及未感病但有其他黄化症状的305株沙田柚树上,采集了590份不同症状的叶片样品,在Nested-PCR检测的基础上,使用广州讯动网络有限公司开发的手持式近红外光谱仪采集光谱,建立标准数据库模型并进行验证。结果表明,以11月307份样品建立的近红外光谱标准数据库模型的检测效果较佳,总识别率81.75%,黄龙病阳性样品和阴性样品的识别率分别为83.41%和78.80%,假阳性率7.62%,假阴性率10.62%。其中,对斑驳黄化叶样品(Nested-PCR检测100%阳性)的识别率最高,达96.72%。在对2月283份样品的验证中,该模型识别能力较差。与Nested-PCR检测相比,近红外光谱检测具有无损、快速的优点,但在对带菌的无症状叶、缺素黄化叶(主要为缺镁)和不带菌的均匀黄化叶和其他黄化叶(主要为烂根、损伤导致的黄化叶)等的检测中灵敏度较低,分别易发生漏检和错检。

关键词：沙田柚黄龙病近红外光谱检测

All-nanofiber self-powered PTFE/PA66 device for real-time breathing monitor by scalable solution blow spinning technology

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Nano Research 2022年第9期15卷 8458-8464页

作者： Pan Li Yibo Liu Han Zhang Zhiping Hu Luna Jia dongkui Liu Lu Yu Bo Li Youwei Yao School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China Institute of Materials Research Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China

All-nanofiber self-powered device was fabricated using simple,low-cost,safe,and scalable solution blow spinning(SBS)technology for real-time respiratory monitor and timely identification of respiratory obstruction ***(PTFE)and polyamide-66(PA66)nanofibers were selected as triboelectric pairs,owing to strong ability to gain electrons of PTFE and supply electrons of ***(ethylene oxide)(PEO)was added to regulate spinning solution viscosity and prepare PTFE/PEO nanofibers,and the morphology and diameter distribution of nanofibers were *** nanofiber film was obtained after the decomposition of PEO in PTFE/PEO nanofiber and melt flow of PTFE pellets in a limited region,and possessed a tensile strength of 1.05 MPa and elongation at a break of 288.58%.Later,PTFE/PA66 all-nanofiber self-powered device was constructed containing PA66 nanofibers,and Au deposition film was used as electrodes by magnetron *** as-obtained device showed robust electrical performance with an open circuit voltage of~110 V at a loading force of 10 N,a short-circuit current of~5 uA at a loading force of 10 N and a frequency of 4 Hz,a maximum power density of 562 mW·m^(–2),and a current of 3.1 uA at a loading resistance of 30 MΩ.Based on the triboelectric mechanism,the device possessed stable response and effective sensibility for stimuli,was used to monitor human breathing conditions,prevent suffocation,and distinguish slow,normal,and fast breathing,with an output voltage of~0.08 V perceived in one normal respiratory circle.

关键词： polytetrafluoroethylene(PTFE) nanofibers triboelectric flexible electronics

延长油田东部浅层致密油储层驱油压裂技术及应用

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断块油气田 2024年第3期31卷 439-443,452页

作者：周东魁余维初周丰樊平天张颖卢毓周吕成成杨森锋长江大学化学与环境工程学院湖北荆州434023 油气田清洁生产与污染物控制湖北省工程研究中心湖北荆州434023 中国石油集团长城钻探工程有限公司北京100101 延长油田股份有限公司南泥湾采油厂陕西延安716000 中国石油集团长城钻探工程有限公司压裂公司辽宁盘锦124000

南泥湾采油厂浅层致密油储层采用滑溜水分段压裂取得初步成效,但产量递减快,原因之一就是油藏能量不足。针对浅层致密油藏增能需求,采用大排量、大液量、低砂比的驱油型滑溜水压裂液滞留地层,补充地层能量,通过焖井进行油水置换,形成驱... 详细信息

南泥湾采油厂浅层致密油储层采用滑溜水分段压裂取得初步成效,但产量递减快,原因之一就是油藏能量不足。针对浅层致密油藏增能需求,采用大排量、大液量、低砂比的驱油型滑溜水压裂液滞留地层,补充地层能量,通过焖井进行油水置换,形成驱油压裂技术。根据施工排量控制总射孔数,按照每孔排量最少0.3 m^(3)/min进行压裂,压后焖井,通过优化压裂缝模拟设计形成较大的储层改造体积,达到体积压裂的效果。评132井区平2井现场试验结果表明,该工艺实施简单,整个施工泵压较低且平稳,排量稳定,取得了较好的增产效果。

关键词：浅层致密油驱油压裂技术驱油型滑溜水压裂液压裂参数优化

Edible prodelphinidins that improve postprandial hyperglycemia were first discovered in Chinese bayberry(Myrica rubra Sieb. et Zucc.) fruits

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Food Science and Human Wellness 2024年

作者： Yi Wang Laiming Zhang Jiaxiong Wu chengxiao Yu Xingqian Ye Haibo Pan Shiguo chen Ningbo Research Institute， Zhejiang University College of Biosystems Engineering and Food Science，Zhejiang Key Laboratory for Agro-Food Processing， Integrated Research Base of Southern Fruit and Vegetable Preservation Technology， Zhejiang International Scientific and Technological Cooperation Base of Health Food Manufacturing and Quality Control， Zhejiang University Zhejiang University Zhongyuan Institute Innovation Center of Yangtze River Delta， Zhejiang University

Plant polyphenols are potential substitutes for clinical α-glucosidase inhibitors. Our previous studies indicated that prodelphinidins from Chinese bayberry leaves (BLPs) have stronger α-glucosidase inhibitory activity than other common polyphenols， but they have no safe history of consumption. There is a reasonable prospect that prodelphinidins from Chinese bayberry fruits (BFPs) can improve postprandial hyperglycemia， though known active components are only myricetin and cyanidin-3-O-glucoside. Hence， the aim of this study was to analyze structure and hypoglycemic effect of BFPs of “Biqi” (BQPs) and “dongkui” (DKPs) cultivars， and compare their difference with BLPs. The contents of BQPs and DKPs were (221.73±0.60) and (136.82±4.33) mg EC equivalents/g dry weight， respectively. The most subunits were EGCG along with a small amount of EGC， ECG and EC connected by B-type linkages and a small portion of A-type linkages with mean polymerization degree of 4.25 and 4.08， respectively. Importantly， BQPs and DKPs were α-glucosidase inhibitors with IC50of 11.91 and 9.47 μg/mL respectively， which were significantly stronger than BLPs. DKPs could also improve postprandial hyperglycemia of normal mice and high fat diet-induced type Ⅱ diabetic mice. Therefore， edible prodelphinidins， which have stronger hypoglycemic effect than BLPs， were first found in Chinese bayberry fruits.

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叙事医学平行病历在外科实习教学中的应用

继续医学教育 2024年第2期38卷 81-84页

作者：吴铁军张宁宋东奎徐玉生郑州大学第一附属医院外科学教研室河南郑州450052

目的探讨叙事医学平行病历书写在医学生外科学实践教学中的应用。方法将2021年7月至2022年6月郑州大学第一临床学院临床医学本科(5+3一体化)180名学生根据随机原则分成叙事医学平行病历教学组和传统教学组,叙事医学平行病历教学组在外... 详细信息

目的探讨叙事医学平行病历书写在医学生外科学实践教学中的应用。方法将2021年7月至2022年6月郑州大学第一临床学院临床医学本科(5+3一体化)180名学生根据随机原则分成叙事医学平行病历教学组和传统教学组,叙事医学平行病历教学组在外科临床实习期间每4周书写1份平行病历,传统教学组以传统教学方法完成实习教学计划,实习结束后测量医患沟通能力、共情能力,问卷调查统计带教老师评价指标、患者满意度。结果实习结束时叙事医学平行病历教学组医患沟通能力评分高于传统教学组[(87.21±5.13)分vs.(73.32±4.37)分],杰弗逊共情量表评分亦高于传统教学组[(103.42±4.48)分vs.(78.32±7.39)分],带教老师对实习医学生工作热情、疾病掌握程度的评价得分分别为(93.85±7.38)分vs.(85.27±5.44)分、(78.51±6.53)分vs.(63.33±7.72)分,均差异有统计学意义(P<0.001)。2组问卷调查患者满意度分别为82.3%、67.8%,差异有统计学意义(P<0.05)。结论将叙事医学平行病历应用在外科实习教学中,能够促进医学生共情能力、医患沟通能力培养,提高岗位胜任力和医学人文素质。

关键词：叙事医学平行病历实习教学医患沟通医学生培养医学人文

Neurofilament 200 expression in a rat model of complete spinal cord injury following growth-associated protein-43 treatment

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Neural Regeneration Research 2009年第11期4卷 827-831页

作者： Yanping Duan dongkui Zhang Yingchun Ba Yun Yuan Jun Sun Dengli Fu Ran Zhang Jinde Wang Department of Histology and Embryology Kunming Medical University Kunming 650031 Yunnan Province China Yunnan Medical College Kunming Medical University Kunming 650031 Yunnan Province China Department of Anatomy Kunming Medical University Kunming 650031 Yunnan Province China Second Affiliated Hospital Kunming Medical University Kunming 650031 Yunnan Province China

BACKGROUND： Growth-associated protein-43 （GAP-43） expression in the nervous system has been demonstrated to promote neural regeneration, neuronal growth and development, as well as synaptic reconstruction. Neurofilament 200 （NF200） expression could reflect degree of injury and repair in injured spinal axons. OBJECTIVE： To observe NF200 expression changes in a rat model of complete spinal cord injury following GAP-43 treatment and to explore the effects of GAP-43 following spinal cord injury. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Laboratory of Histology and Embryology of Kunming Medical University between March 2007 and October 2008. MATERIALS： GAP-43 and GAP-43 antibody were provided by Beijing Boao Biology, China; mouse anti-rat NF200 antibody was purchased from Chemicon, USA. METHODS： Female, 8-week-old, Sprague Dawley rats were randomly assigned into three groups following complete spinal cord injury, with 20 animals in each group： GAP-43 antibody, GAP-43, and model groups. In addition, each group was subdivided into four subgroups according to sampling time after modeling, Le., 3-, 5-, 9-, and 15-day groups, with 5 rats in each group. GAP-43 antibody or GAP-43 was injected into injury sites of the spinal cord, 5 μg/0.2 mL, respectively, twice daily for three consecutive days, followed by three additional days of injection, once daily. The model group did not receive any treatment following injury. MAIN OUTCOME MEASURES： NF200 expression in the damaged spinal area at different stages was detected by immunohistochemistry; lower limb motion function following injury was evaluated using the Basso, Beattie and Bresnahan （BBB） locomotor rating scale. RESULTS： NF200 expression was significantly reduced in the GAP-43 antibody group, compared with GAP-43 and model groups, at 3 and 5 days after spinal cord injury （P 〈 0.05）. In addition, the model group expressed significantly less NF200 than the GAP-43 group （P 〈 0.05）

关键词： spinal cord injury growth-associated protein-43 neurofilament 200 Basso, Beattie and Bresnahan locomotor rating scale

基于贝叶斯优化BP神经网络的压力容器最小壁厚预测研究

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科学技术创新 2024年第22期 69-72页

作者：冯晓刚闫风影沈冬奎韩贯凯山东省安泰化工压力容器检验中心有限公司山东济南

压力容器最小壁厚对压力容器的结构强度和稳定性具有重要作用。鉴于此,利用贝叶斯优化方法结合BP神经网络,实现对压力容器最小壁厚的精确预测。首先,采用贝叶斯方法优化隐含层神经元数量,得到最优隐含层神经元数量。然后,使用该最优神... 详细信息

压力容器最小壁厚对压力容器的结构强度和稳定性具有重要作用。鉴于此,利用贝叶斯优化方法结合BP神经网络,实现对压力容器最小壁厚的精确预测。首先,采用贝叶斯方法优化隐含层神经元数量,得到最优隐含层神经元数量。然后,使用该最优神经元数进行模型训练。最后,将验证集输入到模型中进行验证,以得到压力容器最小壁厚的预测结果。结果表明,基于贝叶斯方法优化BP神经网络的模型能够精确地预测压力容器筒体和封头的最小壁厚,且均方误差仅为0.2678和0.7448,该模型能够提供更可靠的设计和决策依据,为提高压力容器结构设计的效率和安全性做出重要贡献。

关键词：压力容器壁厚贝叶斯优化 BP 预测

基于定性网络模型评价生态调控情景对海洋牧场生态系统的影响——以獐子岛海洋牧场为例

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生态学报 2024年第13期44卷 5761-5772页

作者：孙国庆张合烨董世淇李磊王兆国孙旭李明高东奎田涛吴忠鑫大连海洋大学水产与生命学院大连116023 辽宁省海洋牧场工程技术研究中心大连116023 大连海洋大学设施渔业教育部重点实验室大连116023 獐子岛集团股份有限公司大连116001

在海洋牧场建设过程中,生态调控方式实施后对海洋牧场生态系统的影响通常难以预测,这对海洋牧场的生态安全和高质量发展提出了严峻挑战。为此,建立了一种基于定性网络模型(Qualitative network model,QNM)的海洋牧场生态系统模拟评价方... 详细信息

在海洋牧场建设过程中,生态调控方式实施后对海洋牧场生态系统的影响通常难以预测,这对海洋牧场的生态安全和高质量发展提出了严峻挑战。为此,建立了一种基于定性网络模型(Qualitative network model,QNM)的海洋牧场生态系统模拟评价方法,并以獐子岛海洋牧场近岸增殖海域作为研究区域,构建以增殖目标种为核心的定性网络模型,模拟评估海洋牧场3种不同类型的生态调控情景(增殖目标种、移除捕食者、海藻场修复)及其复合条件下,牧场群落范围内的响应,分析海洋牧场生态调控策略与生物功能群变化之间潜在关系。结果显示:目标种增殖(仿刺参和虾夷扇贝)产生的上行效应导致其捕食者呈现积极响应,产生的下行效应导致其它底栖动物、浮游植物和有机碎屑等功能群呈现消极响应,移除捕食者海星产生的下行效应导致虾夷扇贝呈现积极响应,表明在增殖区清除敌害生物的重要性,海藻场修复对整个群落有明显的积极影响,体现了海藻场在养护近岸生态系统的重要生态意义。研究表明:QNM可有效识别生态系统潜在的营养级联效应,评估生物功能群的响应,基于QNM的海洋牧场生态调控模拟评价方法,突破了定量食物网模型在数据有限系统中使用的局限性,可为海洋牧场建设的生态调控策略制定提供科学参考。

关键词：海洋牧场定性网络模型相互作用增殖移除捕食者海藻场修复